• 대한수의학회지
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• 제30권1호
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• pp.59-64
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• 1990

The present study was conducted in order to elucidate the asymptomatic carrier state of dermatophytes on the laboratory rodents in Korea during the period from August 1986 to April 1987. A total of 988 laboratory rodents, comprising 393 albino rats, 285 albino mice, 238 guinea pigs and 72 hamsters, were randomly selected for the isolation of dermatophytes from 6 research institutes and 2 breeding farms. And the mating experiments were performed to identified the perfect states of Trichophyton mextagrophytes isolated from albino rats. Dermatophytes were recovered from 94 of 393(23.9%) albino rats and from 3 of 5 colonies. Isolation rate of each colony was 38.4%, 32.0% and 9.8%, respectively, and the albino rats over 2 months old(28.2%) were higher than below 2 months old(2.9%) in the isolation rate of dermatophytes. Among 94 strains of dermatophytes isolated from albino rats, all the strains were identified T mentagrophytes, except 1 strain of Microsporum gypseum, and its perfect states were Arthroderma $vanbreuseghemii^+$.

• 대한수의학회지
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• 제28권1호
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• pp.115-118
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• 1988

Mating experiments were performed to, elucidate the distribution of perfect state of Microsporum gypseum complex originated from animals and soils in Korea. A total of 30 strains of M. gypseum complex, composed of 16 from animals and 14 from soils, ere mated with the tester strains of Nannizzia incurvata, N. gypsea and N. fulva. Among 30 strains of M. gypseum complex examined, 16 strains(53.3%) were N. incurvata, 13 strains(43.3%) N. gypsea and 1 strain (3.3%) N. fulva. Among 13 strains of dog isolates, 8 strains(61.5%) were N. incurvata and 5 strains(38.5%) N. gypsea. And each strain isolated from Korean native goat, monkey and albino rat was N. gypsea. Among 14 strains of soil isolates, 8 strains(57.2%) were N. incurvata, 5 strains (35.7%) N. gypsea and 1 strain(7.2%) N. fulva. And -mating type was more frequently observed than +mating type in the strains of N. incurvata and N. gypsea. By this study, N. fulva was isolated the first time in Korea.

• 한국동물학회지
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• 제13권3호
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• pp.68-74
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• 1970

안전방안에서 여포난자가 성숙한다는 것이 몇가지 동물을 이용한 실험에서 밝혀진 바가 있었거니와 여포 난자가 異種의 안전방내에서도 성숙이 가능한 가를 보기 위하여 본 실험을 행하였다. 토끼의 경우 자체의 안전방내에 이식한 여포난자가 다른 동물 즉 흰쥐나 흰생쥐의 안전방에 이식하였을 때보다 낮은 성숙률을 보여 주고 있을 뿐, 흰쥐, 흰생쥐들은 각각 상호간의 안전방에 이식하였을때 同種의 다른 개체의 안정방에서 얻은 결과보다 훨씬 좋은 성숙률을 나타내고 있다. 특히 흰쥐의 여포난자는 숫컷 흰쥐의 안전방에서 24시간 사이에 76%가 제 1및 제 2차 분렬의 증기에 있으며 같은 종류의 수컷 생쥐의 안전방에서 55%가 성숙한 것 보다 훨씬 높은 율을 보여주고 있는 것이다. 안전방의 전방수가 함유단백질의 양이거나 삼투압에 있어서 일반체액이거나 표준배양액의 것들과는 크게 다르며 세포나 조직의 배양액으로는 그다지 적당한 것으로 보이지 않으나 여포난자의 활성획득에는 큰 영향이 없다할 것이다. 특히 異種간의 이식실험에서 얻은 결과가 자체거나 同種간의 이식실험과 거의 같거나 오히려 더 나은 성과를 보여주고 있는데, 는 그 이유에 대해 더 밝혀야 할 문제로 본다. 단지 난자는 성숙분렬을 위한 활성을 갖는데 난자자체의 구성성분 즉 난황물질의 양에 크게 좌우되는 것같으며 또한 난자가 배양될 안전방의 환경요인이 성숙률을 결정하는 데 큰 영향을 주는 것으로 보인다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제12권1호
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• pp.41-61
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• 1990

This study was undertaken to investigate the effect of indomethacin on the distribution of Langerhans cells and T-lymphocytes related with immune response of 4-Nitroquinoline-1-oxide induced carcinogenesis at the palate and tongue of albino rat. 54 Sprague-Dawley strain 10 weeks old albino rats, about 150gm weighted, divided into a normal group of 6 rats without treatment, a control group of 12 rats given indomethacin, a carcinogenesis group of 18 whose palatal mucosa were appiled with 4-Nitroquinoline-1-oxide three times a week, and experimental group of 18 rats were treated with indomethacin and whose palatal mucosa were applied 4-Nitroquinoline-1-oxide. All these 54 rats were subjected to be observed as being ATPase stained specimens, specimens for the observation of light and electron microscope, and T-lymphocyte stained specimens. The obtained results were summarized as follows; 1. In carcinogenesis group, proliferation of epithelial layer and rete peg were observed early period of the experiment and showed parakeratosis, individual cell keratinization, acanthosis, and lymphocyte infiltration from 13th week of the experiment on lightmicroscopically, while experimental group showed less reaction than that of carcinagenesis group. 2. The number of Langerhans cells in normal group rarely changed until 21st week of the experiment, while the Langerhans cells increased markedly from 3rd week of the experiment in control group. 3. The number of Langerhans cells were decreased markedly and persistantly until 21st week of the experiment both in carcinogenesis and experimental groups. 4. Appearance of the T-helper cells and T-suppressor cells were minimal and irregullar in number both in normal and control groups. Thus it is assumed that administration of indomethacin and distribution of Langerhans cells showed close relation. 5. In carcinogenesis and experimental groups, the number of the T-helper cells was apparently inereased than that of the T-suppressor cells, but increasing pattern in experimental group was less than in carcinogenesis group. These cells increased most in the 21st week, decreased from the 23rd week and the appearance of these cells were irregular in general throughout the experiment.

• Parasites, Hosts and Diseases
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• 제27권1호
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• pp.47-56
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• 1989

Centrocestus armatus의 성장 발육 과정을 관찰하기 위하여 피라미(Zacco platypus)를 인공소화시켜 획득한 피낭유충을 100 혹은 200개씩 흰쥐 (Wistar strain)에 경구 감염시키고 1, 2, 3, 4, 6, 8, 14 및 28일 후에 경추탈구법으로 희생시키고 소장을 적출해 내었다. 적출한 소장을 십이지장, 공장, 회장으로 분리하여 0.85% 생리식염수 내에서 열고 장내용물로부터 충체를 회수하였다. 회수된 충체를 10% formalin으로 cover glass 압력하에서 고정하고 Semichon's acetocarmine으로 염색한 다음 광학현미경으로 관찰하고 계측하였다. 그 결과는 다음과 같다. 1. 충체회수율은 평균 10.7%이었다. 감염 1일부터 8일까지는 회수율이 서서히 감소하였고 그 이후에는 28일 까지 관찰한 따 급속히 감소하였다. 2. 회수된 충체의 35.5%는 십이지장에, 62.5%는 공장에 분포하였다. 3. 탈낭된 피낭유충은 길이 $293{\mu\textrm{m}}$, 폭 $144{\mu\textrm{m}}$이었으나, 감염 후 14일에는 길이와 폭이 각각 $382{\mu\textrm{m}}$및 $214{\mu\textrm{m}}$ 로 자라 있었다. 4. 피낭유충은 생식기관 중 고환과 Mehlis선을 가지고 있었으며, 감염 제 1일에는 난소가, 제 2일에는 저정낭과 난환소가 출현하였다. 제 3일 된 충체에는 수정낭이 나타났으며 충란이 형성되었다. 5. 생식기관은 감염 제14일까지 빠른 속도로 발달을 계속하였으며 자궁 내 충란수도 증가하였다. 이상의 결과로 보아 C. armatus는 흰쥐에서 소장 상부에 기생하며 감염 후 3일 만에 성충으로 발육한다는 것을 알았다.

• The Korean Journal of Physiology
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• 제14권2호
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• pp.17-20
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• 1980

This study was undertaken to investigate the effect of the acidosis on the gastric acid secretion in the isolated whole stomach of the rat and the effect of prostaglandin $E_1$ on the gastric acid secretion influenced by the acidosis. Twenty-two male albino rats(Sprague-Dawley strain) were used. The isolated whole stomach from each rat was introduced into the Kreb's solution which was continuously gassed with $95%O_2-5%CO_2$ for 1 hour, after irrigation of the lumen with cold physiological saline$(4^{\circ}C)$. Thereafter, each stomach was irrigated again with 5% dextrose solution (pH 7.4, $37^{\circ}C$), and filled with the dextrose solution. All the stomachs with the dextrose solution were divided into 4 groups according to the Kreb's solutions in which each stomach was incubated for 30 min: 1) control group, in the pH 7.4 solution, 2) $PGE_1$ group, in the pH 7.4 solution containing $5\;{\mu}g/ml$ of $PGE_1$, 3) acid group, in the pH 7.0 solution, and 4) $acid+PGE_1$ group, in the pH 7.0 solution containing $5\;{\mu}g/ml$ of $PGE_1$. After incubatory period, the contents of each stomach were collected and centrifuged(1,500 rpm, room temperature) for 15 min. The acid output in the supernatant was determined with 0.012 N NaOH by means of autotitrator(Dosimat, Metrohm Herisau Co.) at pH 7.4. Results obtained were as follows: 1) The acid output of the acid group increased significantly in comparison with the control value. 2) The acid output of the $acid+PGE_1$ group decreased significantly in comparison with the acid group. It is inferred from the above results that the acidosis facilitates the gastric acid secretion and $PGE_1$ inhibits the gastric acid secretion induced by the acidosis.

• 한국응용과학기술학회지
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• 제2권2호
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• pp.55-61
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• 1985

Our country has been produced much amounts of panax ginseng roots which has a stimulating effects on the metabolism of protein, lipid and nucleic acids in the body. And the leaf trunk of panax ginseng were also produced a considerable amounts as the by - products. Therefore, this study was devised to observe the nutritional effect to rats feeding of rice diet supplemented with by - products of panax ginseng, male Albino rats of pure strain weighing 73.8 ${\pm}$ 0.7 g were used as experimental animal to investigate the changes of cholesterol in heart and testis. The animals were divided into sixteen diet group, they were the protein contents of 9%, 12%, 15% and 18% supplemented with 2% panax ginseng roots and its by - products respectively. The group without the supplements were used as the control. The diet group were again divided into 2 groups according to the feeding terms, 4 weeks and 8 weeks. It is concluded that the free from cholesterol and total cholesterol contents in the heart and testis with the supplements of panax ginseng roots and its by - products showed significant difference compared to the control group.

• Journal of Ginseng Research
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• 제7권1호
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• pp.23-36
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• 1983

This study was devised to observe the nutritional effects of the diets supplemented with the leaf or trunk in rats. The male albino rats (110 heads), Sprague-Dowley strain weighing 75g to 79g, were used as the experimental aninl mils. The animals were divided into twelve diet groups and maintained with corresponding diet for 40 days, and then sacrificed. The growth rate, the consumption and efficiency ratios of the food and protein, the absorption rates of carbohydrate, lipid and protein, and the utilization rates of energy were determined during the feeding term. The results obtained are summarized as follows: 1) The growth rate, the consumption and efficiency ratios of the food and protein in each experimental diet group added ginseng steamed leaf or trunk were higher than those in experimental group A which has the diet supplemented with ginseng raw leaf of trunk. 2) The absorption rates of the carbohydrate were above 96%, higher than those or the control group, But those of fat, protein and the utilization rate of energy in each experimental diet group were generally lower than those in the control group.

• Applied Microscopy
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• 제23권1호
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• pp.56-76
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• 1993

To investigate the histogenesis of tracheal epithelium in Sprague-Dawley strain rat, the author has used the fetal rats at the 16th, 18th, 20th and 22nd prenatal day and neonatal rats at the 1st and 7th day as well as rats at age of 5, 10 and 15 weeks after birth as experimental animals. Specimens were double stained with uranyl acetate and lead citrate for electron microscopic study. The results obtained were as follows; 1. At the 16th day of gestational age, ciliated cells were found in tracheal epithelium and light and dark ciliated cells possessing numerous mitochondria and smooth endoplasmic reticulum in the cytoplasm at the 22nd day of gestational age of the rat are observed. 2. At the 16th day of gestataional age, basal cells lying upon the basement membrane and having large numbers of glycogen particles in the cytoplasm, were found and at the 22nd day of gestational age, basal cells possessing numerous polysomes in the cytoplasm were observed. 3. At the 20th gestational age of the rat, microvillous cells possessing many rough endoplasmic reticulum and mitochondria as well as microvilli protruding into the lumen were found in tracheal epithelium. 4. At the 5th week after birth brush cell having profound filamentous strands and many pinocytic vesicles in the cytoplasm, was visible in the tracheal epithelium. 5. At the 15th week after birth large proportions of tracheal epithelium were lined with ciliated cells. Cosequently it is suggested that pseudostratified ciliated columnar epithelium was differentiated at the 16th day of gestational age, in addition cytoplasmic organelles of the microvillous and basal cells were matured at the 20th and 22nd gestational age, respectively and most of the part of the tracheal epithelium was lined with ciliated cells at the 15th week after birth.

• Parasites, Hosts and Diseases
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• 제30권4호
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• pp.283-288
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• 1992

현재 국내에서 사육 공급되는 실험용 흰쥐의 계통에 따라 감염되는 Pneumocystis carinii(Pc)의 핵형이 다르 다는 사실이 밝혀졌다. 특정 핵형의 병원체를 추적하여 전파를 파악하는 연구의 일환으로, 여러 다른 공급처에 서 얻은 Sprague-Dawley(SD), Fisher(F), Wistar(W) 흰쥐를 여러 장소에서 사육하면서 면역억제하여 Pc를 발현시키고, 이를 순수하게 모아서 field inversion gel electrophoresis를 사용하여 염색체 분자를 분리 관찰하였다. 한 실험실(A)에서 감염을 유발시킨 F 횐쥐는 두 공급원(P, K)에 따라서 같은 실험실에서 사육하였는데도 다른 핵형을 보였다. SD 횐쥐를 M, P, S 세 군데에서 공급받아 서로 격리된 다른 사육실 세 군데에 (A, B, C) 나누어 사육한 결과 P공급원의 횐쥐를 다른 밤 A와 C에서 사육하였는데 같은 핵형(I형)을 보였고, 또한 다른 두 공급원 M과 S에서 구한 SD 계통 흰쥐를 같은 방(B)에서 사육하여 같은 핵형 (II형)을 얻었다. P에서 공급한 F 흰쥐와 M에서 공급한 W 흰쥐를 A 사육실에서 SP 흰쥐와 함께 실험한 결과, F와 SD는 같은 Pc의 핵형 (I형)을 보이고, W는 감염 5주에 B 사육실의 늰퀴와 같은 유형 (II형)을 가졌으나 7주 및 8주에는 II 유형과 I 유형의 복합형을 나타내었다. 이러한 핵형 변화의 유형으로 이들 숙주가 면역억제될 때에는 잠재적으로 가지고 있는 Pc가 발현되나 같은 환경 내에 다른 중감염될 숙주가 있을 경우에는 이 동물로부터 또한 감염을 받는다는 사실을 확인하였다. 특히 상재성 병원체에 감염되지 않게 생산한 동물은 주위의 동물로부터 감염되는 것이 확실하며, 또한 단순하게 같은 방에서 다른 사육조(animal cage)에 사육을 하는 것으로 Pc의 전파가 일어난다는 사실로 미루어 보면, 이 병원체가 공기를 통하여 전파되는 것으로 추정할 수 있었다. (이 연구는 1990년도 서 울대학교 의과대학 발전기금에 의하여 지원되었음)