• 한국식품영양과학회지
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• 제22권4호
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• pp.448-457
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• 1993

혈합육어 중 연근해 온대산 멸치의 내장과 고등어의 유문수, 그리고 원양 열대산 황다랭이 및 날개다랭이의 유문수를 각각 시료로 하여 trypsin을 분리 정제하였으며, 그 분자량과 아미노산 조성 및 효소의 반응 최적조건에 관하여 비교 분석하였다. 실험결과를 요약하면 다음과 같다. 1. 멸치의 내장과 고등어, 황다랭이 및 날개다랭이의 유문수에서 각각 황산암모늄염석, benzamidine-Sepharose 6B 친화성 크로마토그래피, DEAE-Sephadex A-50 크로마토그래피, Sephadex G-75 겔 여과 크로마토그래피 등의 방법을 혼용하여 고등어로부터는 2종의 trypsin (고등어 trypsin A와 고등어 trypsin B로 명명함)을, 그리고 다른 어류로부터는 각각 1종의 trypsin을 분리 정제하였다. 2. 멸치 trypsin과 고등어 trypsin B는 고등어 trypsin A와 황다랭이 trypsin 및 날개다랭이 trypsin에 비하여 그 고유활성이 월등히 높았다. 3. 이들 혈합육어 trypsin의 분자량은 22kDa-26kDa 범위였다. 4. 이들 trypsin은 공통적으로 glycine, serine, aspartic acid를 많이 함유하고 있었으며, tryptophan, methionine, lysine, tyrosine의 함유량은 적었다. 5. BA-$\rho$-NA기질에 대한 반응 최적조건은 멸치 trypsin은 pH 8.0에서 45$^{\circ}C$, 고등어 trypsin A와 B는 pH 8.0에서 5$0^{\circ}C$, 그리고 황다랭이 trypsin은 PH 9.0에서 55$^{\circ}C$, 날개다랭이 trypsin은 pH 9.0에서 5$0^{\circ}C$였다. 6. 위에 든 실험 결과들은 혈합육 어류의 서식온도가 이들 어류의 trypsin의 반응 최적 온도와는 어느 정도 관계가 있을 것으로 추측되었다.

• 한국수산과학회지
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• 제29권1호
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• pp.64-70
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• 1996

멸치, 고등어, 황다랭이 및 날개다랭이의 혈합육어에서 정제된 trypsin을 시료로 하여 각각의 BAPNA기질에 대한 반응속도와 그 관련 성질들을 분석 검토하였다. 4종의 혈합육어에서 정제된 trypsin의 Km'와 $k_{cat}$는 멸치 trypsin이 각각 $49.3{\mu}M$과 $90.9min^{-1}$, 고등어 trypsin A는 $53.7{\mu}M$과 $61.2min^{-1}$, 고등어 trypsin B는 $96.5{\mu}M$ $76.6min^{-1}$, 황다랭이 trypsin은 $62.8{\mu}M$과 $46.4min^{-1}$, 그리고 날개다랭이 trypsin은 $98.3{\mu}M$과 $47.68min^{-1}$ 이었다. TLCK에 대한 $K_i$, 값은 멸치 trypsin이$20.90{\mu}M$, 고등어 trypsin A가 $2.86{\mu}M$, 고등어 trypsin B가 $3.90{\mu}M$, 황다랭이 trypsin이 $0.96{\mu}M$, 그리고 날개다랭이 trypsin이 $1.82{\mu}M$이었으며, 황다랭이의 trypsin이 TLCK에 대하여 가장 예민하게 반응하였다. 이들 trypsin의 효소 활성과 촉매효율은 연근해 온 대산 혈합육어인 멸치와 고등어 trypsin이 열대해역에서 온대해역에 걸쳐 널리 회유하는 혈합육어인 황다랭이와 날개다랭이의 trypsin에 비하여 높은 특징을 보였다.

• 수산해양기술연구
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• 제22권4호
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• pp.21-31
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• 1986

The author investigated the relation between the catches of tuna species and the distribution of horizontal mean temperature at the Jepth of 10m and of vertical temperture sections in the different fishing grounds, using the date of catches in 1980, showing a relative good ones during six years from 1975 to 1980, and of oceanographic observations. Yellowfin and bigeye are mainly caught in South Equatorial Current regions including equatorial upwelling region in 5$^{\circ}$N to 5$^{\circ}$S, and albacore is mainly caught in Subtropical region in 20$^{\circ}$5 to 40$^{\circ}$5. The good fishing grounds of yellowfin and bigeye are made in the depth layer of 100 m to 250 m and temperature of 15$^{\circ}$C to 26$^{\circ}$C having a smooth gradient of thermocline in the Central Pacific between 180$^{\circ}$ and 1500W. But albacore is caught well in which the temperature of thermocline ranges from 100e to 25$^{\circ}$C and its gradient very smoothly. Approaching to the American Continent, the catches of yellowfin and big eye decrease because the thermocline becomes shallower and steeper at Eastern Pacific Region between 1500 and 800W.

• 수산해양기술연구
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• 제50권4호
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• pp.556-566
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• 2014

Korean distant water tuna longline fishery commenced in 1966 in the Atlantic Ocean. Since then, it has become one of the most important fisheries of Korea. By early of 1970s, total amount of tuna caught in the Atlantic Ocean was the highest among other Korean distant water tuna fisheries, but has become minor since 1990s. The annual catch of tuna and tuna-like species by Korean tuna longline fishery in the Atlantic Ocean was about 1,900 mt in 2013. Bigeye tuna was the predominant species in species composition followed by yellowfin tuna, Albacore tuna, Blue shark and Swordfish. Korean distant water tuna longline fishery have mainly operated in the tropical area of the Atlantic Ocean ($20^{\circ}N{\sim}20^{\circ}S$, $20^{\circ}E{\sim}60^{\circ}W$), fishing ground was almost similar as in the previous years. The length frequency of major species (Bigeye tuna, Yellowfin tuna, Albacore tuna, Blue shark and Swordfish) were estimated. As the result of length (size) frequency data on main species caught by lonline fishery in the Atlantic Ocean, main length intervals of bigeye tuna caught in 2011, 2012 and 2013 were 120~125 cm, 160~165 cm and 130~135 cm, respectively. For yellowfin tuna, those were 125~130 cm, 150~155 cm, 145~150 cm and for albacore, 109 cm, 102 cm, 109 cm and 106 cm respectively. For swordfish caught in 2011, 2012 and 2013, main length intervals were 130~135 cm, 125~135 cm and 125~130 cm, respectively, and for blue shark, 195~200 cm in 2011 and 185~190 cm in 2012, 2013.

• 한국식품영양과학회지
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• 제22권4호
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• pp.458-464
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• 1993

전보에 이어, 정제한 혈합육어 trypsin에 대하여 효소적 성질 및 열 역학적 성질에 관하여 비교 검토한 내용을 요약하면 다음과 같다. 이들 혈합육어 trypsin은 BA-p-NA와 SP-p-NA 같은 amide기질 중 BA-p-NA기질만에 대하여, 그리고 ATEE, BAEE, BTEE 및 TAME 등의 ester 기질 중에서는 BAEE와 TAME에 대하여만 현저한 활성을 보였다. 이들 효소는 antipain, leupeptin, DFP, TLCK, SBTI 등 화학약제와 금속이온 Cu$^{2＋}$ 및 Hg$^{2＋}$에 의하여서는 그 활성이 현저히 저해를 받았으나, $Mg^{2＋}$에 의하여서는 부활하였다. 이 효소들은 모두 5$0^{\circ}C$ 이상의 온도에서는 불안정하였으며, 날개다랭이의 trypsin이 온도 변화에 대하여 가장 안정하였다. 그리고, 활성화에너지는 멸치 trypsin이 13.91㎉/mo1e, 고등어 trypsin A는 11.61㎉/ mole, 고등어 trypsin B는 8.43㎉/mo1e, 황다랭이 trypsin은 4.35㎉/mole, 그리고 날개다랭이 trypsin은 3.76㎉/mole 이었다.

• 수산해양기술연구
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• 제32권1호
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• pp.24-32
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• 1996

This paper described on relation between the catches of tuna and the distribution of water temperature of eastern fishing ground of Tropical region in the Pacific Ocean. The data of catches and water temperature used in this paper were based log book which # 27 CHENG RONG(Gross tonnage : 399 ton) had been worked eastern fishing ground(Lat : 09$^{\circ}$N- 14$^{\circ}$S, Long : 115$^{\circ}$- 149$^{\circ}$W)from January to October, 1991. The obtained result are as follows : 1. On the relation between the catches and the geographical distribution, bigeye tuna was higher catches at Lat 4$^{\circ}$- 9$^{\circ}$N, Long 135$^{\circ}$- 139$^{\circ}$W area in the equatorial counter current region where surface water temperature was range of 27.5$^{\circ}C$ to 27.9$^{\circ}C$, yellowfin tuna was higher catches at Lat 4$^{\circ}$- 9$^{\circ}$S, Long 145$^{\circ}$- 149$^{\circ}$W in the south equatorial current region where surface water temperature was range of 28.$0^{\circ}C$ to 28.4$^{\circ}C$ and albacore tuna was higher catches at Lat 10$^{\circ}$- 14$^{\circ}$S, Long 120$^{\circ}$- 124$^{\circ}$W area in the south equatorial current region where surface temperature was range of 26.5$^{\circ}C$ to 26.9$^{\circ}C$ 2. On the relation between catches and distribution of vertical water temperature, bigeye tuna was higher catches at the water temperature of 1$0^{\circ}C$ to 12$^{\circ}C$ on depth layer between 300m and 360m, yellowfin tuna was higher catches at the water temperature of 15$^{\circ}C$ to 19$^{\circ}C$ on depth layer between 180m and 280m and albacore tuna was higher catches at the water temperature of 12$^{\circ}C$ to 14$^{\circ}C$ on depth layer between 280m and 310m. Above the result, it seemed that bigeye tuna distributed deeper layer than yellowfin and albacore tuna.

• 환경위생공학
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• 제6권1호
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• pp.95-102
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• 1991

This study was carried out to determine the contamination level of heavy mentals in canned fishes on the market. Contents of tin, lead, iron and zinc were analyzed by Atomic Absorption Spectrophtometer. Contents of total mercury were determined by mercury analyzer. The results were as follows. 1. The highest value of tin was $1.7706{\pm}0.3216$pprn in canned yellow pin & skip jack(mix) tuna, the lowest was 1.2109 ${\pm}$ 0.0769ppm in canned mackerel pike. 2. The highest value of lead was 0.1657 ${\pm}$ 0.0530 pprn in canned yellow pin tuna, the lowest was 0.0335k 0.0100 pprn in canned mackerel pike. 3. The highest value of iron and zinc was 22.5943 ${\pm}$ 0.7945 ppm and 13.4556 ${\pm}$ 0.6774 pprn in canned sardine fish, the lowest was 4.3514 ${\pm}$ 0.3607 pprn and 3.8773 ${\pm}$ 0.1914 pprn in canned albacore tuna. 4. The total mercury contents was detected 0.0531~0.3386 pprn in three kinds of canned tuna and could not be detected with the others.

• 한국수산과학회지
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• 제51권3호
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• pp.286-297
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• 2018

We analyzed scientific observer data to identify species composition and catch trends of Korean tuna longline fisheries in the Indian Ocean. The data were collected by Korean scientific observers onboard Korean tuna longline fishing vessels in the Indian Ocean from 2010 to 2015. The observers identified 94 species including 5 tuna, 6 billfish, 11 shark, 7 other fish, 5 seabird and 1 sea turtle. Albacore tuna Thunnus alalunga and southern bluefin tuna Thunnus maccoyii were the dominant tuna species, and swordfish Xiphias gladius were the most commonly captured billfish. During the survey periods, 87 seabirds were incidentally bycaught. Black-browed albatross Diomedea melanophris was the dominant seabird species in bycatch, especially in 2010. Cluster analysis showed difference in the species composition of catches between the eastern and western Indian Ocean.

• 한국수산과학회지
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• 제56권4호
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• pp.449-461
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• 2023

The aim of this study was to conduct an ecosystem-based fishery risk assessment of tuna fisheries in the Western Indian Ocean. We selected gillnet, purse seine, hand line, baitboat, and longline fisheries as the target fisheries method, and selected longtail tuna (Thunnus tonggol), narrow-barred Spanish mackerel (Scomberomorus commerson), kawakawa (Euthynnus affinis), skipjack tuna (Katsuwonus pelamis), yellowfin tuna (T. albacares), bigeye tuna (T. obesus), albacore tuna (T. alalunga) and swordfish (Xiphias gladius) as the target species. The risk score for the size at the first capture in sustainability objective was high, especially, for the purse seine and baitboat fisheries using the fish aggregating devices (FADs). The risk score for the bycatch in the biodiversity objective was high for the gillnet fishery, and the gillnet fisheries using FADs showed high risks for the habitat quality objective due to the loss of the fishing gears. With regards to the socio-economic benefits objective, the risk score of the sales profits was low due to high sales of the tuna fisheries. The ecosystem risk score in the Western Indian Ocean was estimated to be moderate, although management is required for some of the indicators that have high-risk scores.

• 수산해양기술연구
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• 제45권1호
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• pp.22-33
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• 2009

Ecological risk assessment(ERA), developed in Australia, can be used to estimate the risk of target, bycatch and protected species from the effects of fishing using limited data for stock assessment. In this study, we employed the ERA approach to estimate risks to tunas, billfishes, sharks, sea turtles and other species by the Korean tuna longline fishery in the Western and Central Pacific Ocean using productivity and susceptibility analyses of the ERA based on low(<1.30), medium(1.30-1.84) and high risk(>1.84). Albacore, bigeye, yellowfin, skipjack and bluefin tunas were generally evaluated in the medium risk. The susceptibility of tuna species, however, had higher risks than the productivity. Billfishes were also at medium risk, while sharks were at high risk by the tuna longline fishery. The risk of productivity was generally high, because most sharks caught by the tuna longline fishery have high longevities, i.e., over 10 years, including ovoviviparous species. Susceptibility, which is related with the selection of fishing gear, was also high, because the longline fishery has no gear modifications to prevent bycatch of protected species. Not only target tuna species were influenced by the tuna longline fishery in the Western and Central Pacific Ocean, but also nontarget species, such as pomfret, mackerels rays, sea turtle were done. Ecosystem-based fishery assessment tools, such as productivity and susceptibility analysis(PSA), have the ability to provide broad scientific advice to the policy makers and stakeholders.