• YAKHAK HOEJI
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• v.51 no.4
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• pp.239-245
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• 2007

In order to evaluate the genotoxicity of airborne particulate matter extracted with dichloromethane (APE), the rat microsome mediated (S-9) or DNA repair enzyme treated Comet assays were performed using the single cell gel electrophoresis in A549 human lung carcinoma cells. It was found that the cells interacting with APE showed more DNA single-strand breaks relative to untreated cells. The genotoxicity of APE was increased with the treatment of S-9 mixture. Microsome mediated DNA damage was inhibited by CYP1Al inhibitor, quercetin. The APE also showed oxidative DNA damage evaluated by endonuclease III treatment. Oxidative DNA damage of APE was inhibited by antioxidants such as vita- min C and Trolox. We also found that the vegetables or fruits extract may reduce APE-induced genotoxicity by their anti- oxidant activity and CYP1A1 inhibition.

• Asian Journal of Atmospheric Environment
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• v.4 no.1
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• pp.1-8
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• 2010

The effectiveness of the government regulation on tail-pipe emission for diesel vehicles issued in 2003 in Tokyo was evaluated in this study. Variations in annual average concentrations of polycyclic aromatic hydrocarbons (PAHs) and nitro-PAHs associated with airborne particulate matter were investigated in connection with the variation in airborne elemental carbon (EC) concentration in downtown Tokyo, Japan in 2006-2007 and in 1997-1998. The annual average concentrations of EC, seven different PAHs, and 1-nitropyrene were found to have decreased significantly from 1997-1998 to 2006-2007. The most prominent decrease in atmospheric concentration was observed for 1-nitropyrene, which is a representative nitro-PAH originating from diesel vehicles. This indicated that the government control has worked to considerably reduce both atmospheric mutagens and airborne particulate matter. In contrast, the concentrations of two nitro-PAHs, 2-nitrofluoranthene and 2-nitropyrene, remained the same. These nitro-PAHs are known to be formed by atmospheric nitration of their parent PAHs, and this result suggested factors other than the concentration of parent PAHs and $NO_2$ affects the degree of atmospheric formation of nitro-PAHs.

• Journal of Preventive Medicine and Public Health
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• v.39 no.4
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• pp.325-330
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• 2006

Objectives: The aims of this study were to examine the level of airborne fungi and environmental factors in Seoul metropolitan subway stations and to provide fundamental data to protect the health of subway workers and passengers. Methods: The field survey was performed from November in 2004 to February in 2005. A total 22 subway stations located at Seoul subway lines 1-4 were randomly selected. The measurement points were subway workers' activity areas (station office, bedroom, ticket office and driver's seat) and the passengers' activity areas (station precincts, inside train and platform). Air sampling for collecting airborne fungi was carried out using a one-stage cascade impactor. The PM and CO2 were measured using an electronic direct recorder and detecting tube, respectively. Results: In the activity areas of the subway workers and passengers, the mean concentrations of airborne fungi were relatively higher in the workers' bedroom and station precinct whereas the concentration of particulate matter, $PM_{10}\;and\;PM_{2.5}$, were relatively higher in the platform, inside the train and driver's seat than in the other activity areas. There was no significant difference in the concentration of airborne fungi between the underground and ground activity areas of the subway. The mean $PM_{10}\;and\;PM_{2.5}$ concentration in the platform located at underground was significantly higher than that of the ground (p<0.05). Conclusions: The levels of airborne fungi in the Seoul subway line 1-4 were not serious enough to cause respiratory disease in subway workers and passengers. This indicates that there is little correlation between airborne fungi and particulate matter.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.25 no.2
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• pp.194-201
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• 2015

Objectives: The purpose of this study is to assess the exposure level to dust and airborne microorganisms among employed workers in a clothing shopping center. Materials and Methods: On-site investigation of a clothing shopping center was performed between October and November 2012. The hazardous substances measured in this study are particulate matter(Total dust, respirable dust) and airborne microorganisms (Total airborne bacteria, total airborne fungi). Results: The highest geometric mean levels of particulate matter(total dust, respirable dust) for personal sampling were $1.735(SD:0.883)mg/m^3$ for total dust and $0.0711(SD:0.008)mg/m^3$ for respirable dust, respectively. Those for area sampling were $0.625(SD:0.091)mg/m^3$ for total dust and $0.0718(SD:0.012)mg/m^3$ for respirable dust, respectively. The highest geometric averaged concentrations of airborne microorganisms(Total airborne bacteria, total airborne fungi) were detected at $1,181(SD:105)cfu/m^3$ for total airborne bacteria and $683(SD:114)cfu/m^3$ for total airborne fungi, respectively. Concentrations of particulate matters and airborne microorganism in clothes shopping center did not correlate significantly with environmental factors such as temperature or relative humidity. Conclusions: Exposure levelshave not been established for service workers. Thus, health risk assessment for this group is very difficult. Health guidelines for service workers should be established as soon as possible.

• YAKHAK HOEJI
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• v.33 no.1
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• pp.46-53
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• 1989

The extractable organic matter was extraced from airborne particulates collected in Seoul during 1986. It was fractionated to several fracrtions and mutagenicities of them were tested in Salmonella thyphimurium TA98 by Ames method. The neutral fraction showed the highest indirect acting mutagenicity while the highest direct acting mutagenicity was observed in the acidic fraction. Indirect acting mutagenicity of airborne particulate was attributed to its neutral fraction about 88% in average, to acidic about 10% in average and to basic fraction about 2% in average. Direct acting mutagenicity of airborne particulate was attributed to its neutral fraction about 70%, to acidic fraction about 29% and to basic fraction about 1%. Among five subfractions of neutral fraction, the proportion of mutagenicity of polynuclear aromatic hydrocarbon subfraction was 13.2% in indirect acting mutagenicity and 5.0% in direct acting mutagenicity.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.165.1-165.1
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• 2003

This research was designed to examine the presence of mutagenic/carcinogenic compounds in airborne pollutants in diesel particulate matter using an integrated biological approach. Respirable air borne particulate matter (PM2.5: <2.5mm) was collected from diesel engine exhaust using a high-volume sampler equipped with a cascade impactor. (omitted)

• Analytical Science and Technology
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• v.18 no.1
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• pp.51-58
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• 2005

A comparison of the analytical data obtained by three $k_0$-NAA software programs was carried out using both the airborne particulate matter collected from an urban site and the certified reference materials of the air filter and urban dust to evaluate the performance of the analysis. The individual $k_0$-NAA standardization methods of three countries, Korea, China and Vietnam which had been modified from the well established $k_0$-program were used for the comparative analysis. The measured concentrations of 30 elements from the two kinds of air samples based on this software were in agreement with each other within about 20% analytical error except for a few elements. By contrast, the results of China and Vietnam were moderately higher than that of Korea due to a systematic error associated with the detection efficiency, gamma peak analysis and geometric effect.

• Nuclear Engineering and Technology
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• v.36 no.5
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• pp.450-459
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• 2004

For the evaluation of emission sources by air sampling, airborne particulate matter for fine (<2.5 ${\mu}m2$ EAD : $PM_{2.5}$) and coarse partical (2.5-10 ${\mu}m2$ EAD : $PM_{2.5-10}$ fractions were collected using a Gent stacked filter unit low volume sampler and two types of polycarbonate filters. Air samples were collected twice monthly at two regions in and around Daejeon city in the Republic of Korea from January to December 2002. Monthly mass concentration of $PM_{2.5}$ and $PM_{2.5-10}$ were measured and the concentrations of 10 marker elements (Al, Sc, Ti ; Na, Cl ; As, V. Sb, Br, Se) were determined by an instrumental neutron activation analysis. Analytical quality control was corried out using certified reference materials. Enrichment factors were also calculated from the monitoring data to classify the anthropogenic and crustal origins.

• Fisheries and Aquatic Sciences
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• v.27 no.2
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• pp.111-121
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• 2024

Particulate matter (PM) is a mixture of microscopic solid inhalable particles including airborne liquid droplets, and it is implicated with several diseases. The eye does not have a protective barrier among the human organs, consequently it get directly exposed to environmental substances such as PM. The scarcity of treatments for damage to the eyesight and the vision and eye structure being closely related to the structure and function of the central nervous system highlights the cruciality of novel therapeutics. In this study was conducted using in vivo zebrafish vertebrate model which is a useful model due to the conserved genes between human. We found that protective effect of Octopus minor extract against particulate matter-induced adverse effects on eye development in zebrafish (Danio rerio) embryos by regulating antioxidant and anti-inflammatory mRNA expression.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.27 no.1
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• pp.70-76
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• 2017

Objectives: This on-site study was performed to evaluate the reduction efficiency of an air cleaner on particulate matters and biological agents in a swine facility. Materials and Methods: Particulate matter was measured using a real-time monitoring recorder and biological agents were sampled with a one-stage impactor and then analyzed based on the microbial culture method. An experimental process for the reduction effect on airborne pollutants through air cleaner operation consisted of three conditions: no treatment, wet scrapper by water spray and wet scrapper by disinfectant spray. Results: Geometric mean levels of particulate matter(TSP, $PM_{10}$, $PM_{2.5}$ and $PM_1$) were presented at $1,608{\mu}g/m^3$, $1,373.8{\mu}g/m^3$, $401.8{\mu}g/m^3$ and $144.5{\mu}g/m^3$ for no treatment; $1,503{\mu}g/m^3$, $1,017{\mu}g/m^3$, $159.4{\mu}g/m^3$ and $69.8{\mu}g/m^3$ for wet scrapper by water spray; and $1,222.17{\mu}g/m^3$, $477.17{\mu}g/m^3$, $33.2{\mu}g/m^3$ and $11.1{\mu}g/m^3$ for wet scrapper by disinfectant spray, respectively. In the case of biological agents, the geometric averaged concentrations of total airborne bacteria and fungi were as follows: $45,371cfu/m^3$ and $13,474cfu/m^3$ for no treatment, $43,286cfu/m^3$ and $8,610cfu/m^3$ for wet scrapper by water spray, and $2,440cfu/m^3$ and 1,867 cfu/ for wet scrapper by disinfectant spray, respectively. Regardless of particulate matter and biological agent, the highest concentrations were found for no treatment, while the lowest concentrations were found with wet scrapper by disinfectant spray. Conclusions: Based on the results obtained from this on-site evaluation, there was a significant reduction effect on particulate matter and biological agents through the application of an air cleaner in this study.