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Mass Proliferation of Hibiscus hamabo Adventitious Root in an Air-lift Bioreactor, and the Antioxidant and Whitening Activity of the Extract (생물반응기를 이용한 황근 부정근의 대량증식과 추출물의 항산화 및 미백 활성 평가)

  • Lee, Jong-Du;Hyun, Ho Bong;Hyeon, Hyejin;Jang, Eunbi;Ko, Min-Hee;Yoon, Weon-Jong;Ham, Young Min;Jung, Yong-Hwan;Choi, Hwon;O, Eu Gene;Oh, Daeju
    • Korean Journal of Plant Resources
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    • v.35 no.4
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    • pp.435-444
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    • 2022
  • Hibiscus hamabo Sieb. et Zucc. (yellow hibiscus) is a deciduous semi-shrub plant and mainly growing in Jeju Island. This is known the unique wild hibiscus genus and classified as an 2nd grade of endangered plant for Korean Red List. In previous studies, properties of germination, ecological, genetical and salt resistance have been reported. In this study, we investigated mass-proliferated adventitious root using bioreactor, antioxidant and whitening effects to conduct functional ingredients. Yellow hibiscus were collected from Gujwa, Jeju by prior permission and they were introduced by explant type and various medium composition after surface sterilization. As a result, seed response rates were evaluated at range of 51.17~51.83%, in terms of comprehensive efficiency of shoot and root formation. In the case of adventitious root propagation condition was confirmed in half strength Murashige and Skoog medium salts, 30 mg/L sucrose, and 2 mg/L indole-3-butyric acid for 8 weeks in 5,000 mL bioreactor. We also compared between relationship with biomass and secondary metabolites accumulation by total phenolics content, the flavonoid content, DPPH free radical scavenging activity and melanin content. The results indicated that adventitious root mass proliferation, antioxidant and whitening effect could develop value of the high-quality cosmeceutical ingredient and further metabolite studies.

Biological Yielding Potential of Rice in Association with Climatic Factors in Yeongnam Region (영남지역 기상과 수도의 한계생산력 해석)

  • Kim, Soon-Chul;Lee, Soo-Kwan;Chung, Geun-Sik
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.3
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    • pp.259-270
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    • 1985
  • Meteorological year variations for rice crop from 1973 to 1984 were compared by using air temperature and sunshine hour for nursery period, cooling index for reproductive stage and meteorological yield productivity index for ripening period. The most optimum transplanting date and heading date for crop yield based on real transplanting date-grain yield relationship or heading date-grain yield relationship, meteorological yield productivity index and actual results showed good agreement each other. Around May 26 for transplanting and August 10 for heading were the most optimum date in Indica/Japonica hybrid cultivars while these were about June 8 and August 23 for Japonica cultivars, respectively. On the other hand, theoretical late limiting heading date for safe ripening were August 20 for Indica/Japonica hybrid cultivars and August 30 for Japonica cultivars, respectively, for both methods, cumulative temperature method during ripening with 80% believable frequency and meteorological yield productive index method having 1000(kg/10a) yielding potential. Based on the yield forecast trial, the highest values of photosynthetic efficiency, 2.5%, and crop growth rate, 23g/㎡/day, were recorded during 30 days before rice heading. Considering the photosynthetic efficiency and solar radiation, the potential crop growth rate was more or less 30g/㎡/day and the biological grain yielding potential in a existing cultural practices was approximately 900-1000(kg/10a) in Milyang weather condition. To increase further yielding potential, either photosynthetic efficiency or harvest index or both should be improved by manipulating appropriate canopy architecture, plant spacing, fertilizer, chemical, etc.

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Effect of Soil Strength on Seedling Emergence of Rice and Barnyardgrasses in Direct Dry-Seeding (건답직파에서 토양경도가 벼와 피의 출아에 미치는 영향)

  • Kwon, Yong-Woong;Lee, Byun-Woo;Kim, Do-Soon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.4
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    • pp.489-495
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    • 1996
  • Seedling emergences of four rice varieties (Dongjinbyeo, Dadajo, Galsaekggarak-sharebyeo, and Italiconaverneco) and three barnyardgrass species(Echjnochloa oryzjcola, E. crus-gali var. crus gali E. crus-gali var. praticola) were evaluated in relation to soil strength. Soil strength was varied by compressing the entire volume of soil with a hydraulic jack so as to be 0.5, 1, 2, 3, 6kg /$\textrm{cm}^2$. Soil strength was measured with a penetrometer (Yamanaka type) and soil covering above the seed was 4cm deep. Experiments were conducted at two air temperature conditions of 17 and $25^{\circ}C$. At a soil strength of up to 2kg/$\textrm{cm}^2$, little or no decrease in seedling emergence occurred in all rice varieties and barnyardgrasses tested. Above that value, seedling emergence decreased progressively as the soil strength increased. The degree of decrease was greatest in Dongjinbyeo and smallest in Dadajo among tested rice varieties, and greatest in Echinochloa oryzicola among barnyardgrasses, being greater in barnyardgrasses than rice. Seedling emergence was delayed almost linearly as the soil strength increased. The delay was greatest in Dongjinbyeo among rice varieties and in Echinochloa oryzicola among barnyardgrasses. Mesocotyl length increased as soil strength increased up to 2 to 3kg / $\textrm{cm}^2$ in Dongjinbyeo and Dadajo in 17$^{\circ}C$ and $25^{\circ}C$, and up to 6kg/$\textrm{cm}^2$ in Galsaekggaraksharebyeo and Italiconaverneco in $25^{\circ}C$. Dongjinbyeo showed the least elongation of mesocotyl among rice varieties in any soil strength. The total length of mesotyl, first internode and incomplete leaf showed little variation with soil strength. The total length was longer than the 4cm covering depth in other varieties except Dongjinbyeo. This might have caused the lower emergence rate in Dongjinbyeo than other varieties in higher soil strength.

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Transition of Rice Culture Practices during Chosun Dynasty through Old References IV. Preparation of Seeds and Land (주요 고농서를 통한 조성시대의 도작기술 발전 과정 영구 IV. 조선시대의 비곡종 및 경지관리)

  • Lee, Sung-Kyum;Guh, Ja-Ok;Lee, Eun-Woong;Lee, Hong-Suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.6
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    • pp.576-585
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    • 1991
  • General procedures of seed preparation as conventional guide had been established in China before most of Korean literature documented them. ‘Chwijongbeob’ (method of seed select) was to select good quality of seeds and to discard the rest. In ‘Seonjongbeob’ (method of seed grading) although China employed only ‘Sooseonbeob’ (method of seed select with water), but seeds were selected in order of selection of seeds by winds, selection of seeds by sieve and selection of seed with water in Korea. As compared with the recent techniques, those methods were perfect techniques for selection of good quality seeds of rice, except for method of seed selection by salt water was developed. The method for measurement of seed moisture, and for measurement of melted snow, spoiled urine and extracted juice by boiling water with the bone of livestock were originated from ancient China. The farming books in Korea were more or less followed the above methods. However, these techniques were complicated and impractical interms of validity and rationality. Also, it is judged that these tchniques are more appropriate in dry areas and alkaline soil of China rather than in Korean conditions. The plowing is a work to begin farming, and is operated for air ventilation between atmosphere and earth. Also, this techniques was adopted in the farming books from the early to the late Chosun dynasty without changes. Fields were deep-plowed in the first, in fall (or in spring) and for cultivation, and were shallow -plowed in the second, in spring (or in summer) and in intertillage. The former was for water reserve and land preparation, and the later was for weed control with intertillage. However, plowing in fall which was different from fallowing in dry areas, was recommended in Korea (Jikseol). but was not practiced in Sejongsilrok. This was changed with time, and plowing for cultivation in Korea was interrelated with use of green manure crops, method of plowing of upseting plough, method of manure practice and sometimes dry plowing. In addition, until the 15th century method of using a kind of plowing-tool made of log as farm tools was created to support reclamation for enlargement of farm land in mountaineous and coastal areas. For desolate farm lands by many internal and external disturbances, one tried to recover yield ability by increasing labor productivity from the 17th or 18th century. To do this, ‘Banjongbeob’ (culture method by upset plowing weed control) and ‘Hwanubeob’(culture method by firing weed control) which were cultural methods of ancient China were readapoted but the results were not clearly informed. Also, the reality of those was reexamined in the end of the Chosun dynasty.

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Studies on the Reinforced Effect of Rubber Elastomer by means of Milled Glass Fiber Treated with Silane Coupling Agents (Silane Coupling제(劑) 처리(處理) Glass Fiber에 의(依)한 탄성체(彈性體)의 보강효과(補强效果)에 관(關)한 연구(硏究))

  • Lee, Sang-Hyun;Yoo, Chong-Sun;Paik, Nam-Chul
    • Elastomers and Composites
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    • v.22 no.3
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    • pp.204-212
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    • 1987
  • The purpose of this study is to investigate the reinforced effect between MGF treated silane coupling agents and rubber matrix under the configuration chemical bonds, also the effect of triazine thiol compounds. For this study, vulcanizates were prepared with fifteen different compounding formulas. Their vulcanization characteristics, physical properties were examined by means of the ODR(Oscillating Dist Rheometer), the tensile tester, the benzene swelling test. The results of this study obtained are as follows: 1. In the ODR test, the MA vulcanizate was the fastest one in terms of having reached to optimum cure time($t_{90}$) and, with the same formula, when MGF vulcanizates, the shortest optimum cure times has appeared. 2. The SA, SC vulcanizates were the best the other in the physical properties such as 100%modulus, 200%modulus, 300%modulus, tensile strength. The SB vulcanizate, with higher density of crosslinking than other vulcanizates. The vulcanizates, which were filled with MGF treated with silane coupling agents we were the higher density of crosslinking than vulcanizates filled with MGF only. 3. In aging properties, the silica vulcanizates appeared to be better than the other vulcanizates. The aging Properties of treated MGF vulcanizates were similar to the silica vulcanizates. The(CR+APS+silica) and(CR+APS+MCF) were easily crosslinked by exposure to the air, and the physical properties have been improved.

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Effects of Soil Crusting and Hardening during Drying after Artificial Rainfall on Seedling Emergence of Rice and Barnyardgrass (강우처리후 토양건조에 따른 피막형성 및 경도변화가 벼와 피의 출아에 미치는 영향)

  • Lee, Byun-Woo;Kwon, Yong-Woong;Myung, Eul-Jae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.2
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    • pp.131-138
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    • 1996
  • Soil crusting and hardening as a result of drying after rainfall were examined in relation to seedling emergence by employing five rice varieties (Italiconaverneco, Dadazo, and Galsaekggarakshare, Dongjinbyeo and Sumjinbyeo) and two barnyardgrass species (E. crus-gallj var. oryzjcola and E. crus-galli var. praticola). Sandy loam, loam, and silty loam soils were used. The artificial rainfall of 0, 20 and 40mm were applied after sowing and covering with 4cm soil. Air temperature and solar radiation averaged over 9 days after seeding was 31.3$^{\circ}C$ and 16.9MJ /m$^2$, respectively. Soil strength increased rapidly by drying after artificial rainfall, being greater in soils with greater amount of clay and artificial rainfall. Soil crust was formed on the surface with artificial rainfall in all soils tested. However, soil crust was exfoliated in silty loam and loam soil, and lifted as seedlings emerge. Seedling emergence of rice varieties was decreased by rainfall treatments. Sumjinbyeo and Dongjinbyeo showed much poorer seedling emergence especially in sandy loam soil than the other varieties. Poor seedling emergence of these varieties might have been caused by delayed seedling emergence which had made them expose to greater soil strength. Seedling emergence of barnyardgrasses showed no differences among soil textures and rainfall treatments, because they emerged rapidly before soil crusting and hardening were proceeded enough to hamper seedling emergence. Seedling emergence of Sumjinbyeo and Dongjinbyeo decreased with increasing soil strength averaged over 3 days to 5 days after seeding, being lowered to 80% at soil strength of 1.0kg/cm$^2$ and to 50% at 1.7kg/cm$^2$. Emergence speed of barnyardgrasses was faster than rice varieties, and E. crus-galli var. oryzjcola than E. crus-galli var. praticola. Italiconaverneco and Dadazo showed faster emergence in rice varieties. Galsaekggarakshare showed slower emergence speed than these two varieties with similar seedling emergence percentage. The greater and faster elongations of mesocotyl and incomplete leaf in rice, and of mesocotyl in barnyardgrass were the characteristics responsible for higher seedling emergence rate in the environment examined.

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Diversification of Rice Quality for Processing. Physicochemical Characteristics and Inheritance of Floury Endosperm Mutants (특수 가공용 미질개발 : 분상질배유 돌연변이 계통의 이화학적특성과 유전)

  • Kim, Kwang-Ho;Koh, Hee-Jong;Lee, Jang-Hoon;Park, Sun-Zik;Heu, Mun-Hue
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.3
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    • pp.264-274
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    • 1993
  • This study was carried out to assess the agronomic characters and physicochemical properties of floury and chalky-endosperm mutant lines induced by chemical mutagen treatment to rice varieties, Hwacheongbyeo and IR24. Linkage analysis of a floury-endosperm gene was carried out using linkage testers. The grain size of brown rice of the mutants was smaller than that of the original varieties. The l, 000-grain and 1$\ell$ weight were lighter in the mutants compared with those in the original varieties. The compound starch granules in the endosperm cell of the mutants showed a loosely-packed crystalline structure. Amylose contents in mutants ranged from 16.9 to 28.5%. Crude protein contents of the mutants were not significantly different from the original rice variety, Hwacheongbyeo, but white core mutant(line 47106) derived from IR24 showed higher protein(l1.32%) compared with IR24(8.30%). The mutants showed slightly harder gel characteristics, and much lower viscosity in Amylograph than original varieties. Steamed rice-cakes from mutant lines showed greater volume than those from original varieties. During the process of alcohol fermentation, Brix in the mutants(especially floury mutants) decreased faster and the alcohol production after 10-day fermentation was much greater in the mutants than in the original varieties. Three different gene loci for floury endosperm characteristics were identified from the allelism test among mutant lines, and the genes were tentatively symbolized as flo-a, flo-b and flo-c, respectively. A floury gene, flo-a, was linked with lg(liguleless) gene in the linkage group N, with R.V. 5.76$\pm$1.72%.

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Chinese Maritime Dispute Strategy for territorialization in Korea's West Sea (중국의 한국 서해 내해화 전략 분석)

  • Lee, Eunsu;Shin, Jin
    • Maritime Security
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    • v.5 no.1
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    • pp.113-136
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    • 2022
  • China has been pushing for a systematic strategy for territorialization over a long period of time to invade Korea's West Sea (Yellow Sea) in order to create China's territorial water. China's strategy for territorializing the West Sea is an activity in which China curbs the use of South Korea and enforces the illegal use of China in order to dominate the West Sea exclusively. China aided Chinese fishing boats that engaged in illegal fishing in Korea's jurisdiction as a means to territorialize the West Sea, and is opposed to combined exercise and training of Korea and the United States Naval Forces in the West Sea, while intentionally entering KADIZ(Korea Air Defense Identification Zone). In addition, Beijing used 'scientific exploration and research' measures as a pretext for its strategies in order to encroach on Korea's West Sea. China is carrying out such work to announce to the world that China is a systematic and organized country while consistently attempting to dominate the West Sea. China's activities in the West Sea seriously infringe South Korea's sovereignty. In order to respond to China's strategies of territorialization in the West Sea stated above, I analyzed the rejection effect of the ROK-US combined military training in the West Sea and presented a 'proportional response strategy centered on the ROK-US combined forces'. Korea should be able to respond proportionally to China's activities in the seas around the Korean peninsula, and Korea should be able to neutralize China's attempt to a Fait Accompli. In addition, just as China installs buoys in the Korea-China Provisional Measures Zone, Korea should be able to install and actively utilize some devices in the West Sea and for the use of free and open West Sea. Korea should not just wait for the tragic future to come without preparing for China's gradual and long-term strategy, and Seoul needs to respond to China's maritime policy in the West Sea with a more active attitude than it is now. China has historically taken a bold and aggressive response to neighboring countries that are consistent with a passive attitude, on the other hand, Beijing has taken a cautious approach to neighboring countries that respond with an active attitude. It should not be forgotten that Korea's passive response to the Chinese strategy in the name of a 'realistic approach' such as Korea's economic dependence on China for economy will result in China's success for territorialization of the West Sea.

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Exploring a Balanced Share of Slow Charging Options by Places Based on Heterogeneous Travel and Charging Behavior of Electric Vehicle Users (장소별 완속충전기 적정 보급 비율에 관한 연구 : 전기차 이용자의 통행 및 충전행태에 따른 이질성을 중심으로)

  • Jae Hyun Lee;Seo Youn Yoon;Hyeonmi Kim
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.21 no.6
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    • pp.21-35
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    • 2022
  • With the support of local and central governments, various incentive policies for "green" cars have been established, and the number of electric vehicle users has been rapidly increasing in recent years. As a result, much attention is being given to establishing a user-centered charging infrastructure. A standard for the number of electric vehicle chargers to be supplied is being prepared based on building characteristics, but there is quite limited research on the appropriate ratio of slow and fast chargers based on the characteristics of each place. Therefore, this study derived an appropriate penetration ratio based on data about the distribution ratio of common slow chargers. These data were collected using a survey of actual electric vehicle users. Next, an analysis was done on how to categorize the needs of charging environments and to determine what criteria or characteristics to use for categorization. Based on the results of the survey analysis, three types of places were derived. Type-1 places require 10% of chargers to be slow chargers, Type-2 places require 40-60% of chargers to be slow chargers (i.e., around equal distribution of slow and fast chargers), and Type-3 places require more than 80% of chargers to be slow chargers. The required levels of slow chargers were classified by place type and by individual using latent class cluster analysis, which made it possible to categorize them into five clusters related to socioeconomic variables, vehicle characteristics, traffic, and charging behaviors. It was found that there was a high correlation between charging behavior, weekend travel behavior, gender, and income. The results and insights from this study could be used to establish charging infrastructure policies in the future and to prepare standards for supplying charging infrastructure according to changes in the electric vehicle market.

Influence of Land Cover Map and Its Vegetation Emission Factor on Ozone Concentration Simulation (토지피복 지도와 식생 배출계수가 오존농도 모의에 미치는 영향)

  • Kyeongsu Kim;Seung-Jae Lee
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.25 no.1
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    • pp.48-59
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    • 2023
  • Ground-level ozone affects human health and plant growth. Ozone is produced by chemical reactions between oxides of nitrogen (NOx) and volatile organic compounds (VOCs) from anthropogenic and biogenic sources. In this study, two different land cover and emission factor datasets were input to the MEGAN v2.1 emission model to examine how these parameters contribute to the biogenic emissions and ozone production. Four input sensitivity scenarios (A, B, C and D) were generated from land cover and vegetation emission factors combination. The effects of BVOCs emissions by scenario were also investigated. From air quality modeling result using CAMx, maximum 1 hour ozone concentrations were estimated 62 ppb, 60 ppb, 68 ppb, 65 ppb, 55 ppb for scenarios A, B, C, D and E, respectively. For maximum 8 hour ozone concentration, 57 ppb, 56 ppb, 63 ppb, 60 ppb, and 53 ppb were estimated by scenario. The minimum difference by land cover was up to 25 ppb and by emission factor that was up to 35 ppb. From the modeling performance evaluation using ground ozone measurement over the six regions (East Seoul, West Seoul, Incheon, Namyangju, Wonju, and Daegu), the model performed well in terms of the correlation coefficient (0.6 to 0.82). For the 4 urban regions (East Seoul, West Seoul, Incheon, and Namyangju), ozone simulations were not quite sensitive to the change of BVOC emissions. For rural regions (Wonju and Daegu) , however, BVOC emission affected ozone concentration much more than previously mentioned regions, especially in case of scenario C. This implies the importance of biogenic emissions on ozone production over the sub-urban to rural regions.