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Genetic Diversity and Relationship of Ogye Population in Korea Using 25 Microsatellite Markers (MS 마커를 활용한 지역별 오계 유전자원의 다양성 및 유연관계 분석)

  • Roh, Hee-Jong;Kim, Kwan-Woo;Lee, Jin-Wook;Jeon, Da-Yeon;Kim, Seung-Chang;Jeon, Ik-Soo;Ko, Yeoung-Gyu;Lee, Jun-Heon;Kim, Sung-Hee;Baek, Jun-Jong;Oh, Dong-Yep;Han, Jae-Yong;Lee, Seung-Sook;Cho, Chang-Yeon
    • Korean Journal of Poultry Science
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    • v.45 no.3
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    • pp.229-236
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    • 2018
  • The aim of this study was to evaluate the genetic diversity and relationships of Ogye populations in Korea. A total of 243 genomic DNA samples from 6 Ogye population (Yeonsan Ogye; YSO, Animal Genetic Resources Research Center Ogye; ARO, Chungbuk Ogye; CBO, Chungnam Ogye; CNO, Gyeongbuk Ogye; GBO, Seoul National University Ogye; SUO) and 3 introduced chicken breeds (Rhode Island Red; RIR, White Leghorn; LG, Cornish; CN) were used. Sizes of 25 microsatellite markers were decided using GeneMapper Software(v 5.0) after analyzing ABI 3130XL. A total of 153 alleles were observed and the range was 2 to 10 per each locus. The mean of expected and observed heterozygosity and PIC (Polymorphism Information Content) value was 0.53, 0.50, 0.46 respectively. The lowest genetic distance (0.073) was observed between YSO and SUO, and the highest distance (0.937) between the RIR and CBO. The results of clustering analysis suggested 3 clusters (${\Delta}K=7.96$). Excluding GBO population, 5 Ogye populations (YSO, ARO, CBO, CNO, SUO) were grouped in same cluster with high genetic uniformity (0.990, 0.979, 0.989, 0.994, 0.985 respectively). But GBO population was grouped in cluster 1 with low genetic uniformity (0.340). The results of this study can be use to basic data for the genetic evaluation and management of Ogye populations in Korea.

Growth and Physiological Adaptations of Tomato Plants (Lycopersicon esculentum Mill) in Response to Water Scarcity in Soil (토양 수분 결핍에 따른 토마토의 생육과 생리적응)

  • Hwang, Seung-Mi;Kwon, Taek-Ryun;Doh, Eun-Soo;Park, Me-Hea
    • Journal of Bio-Environment Control
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    • v.19 no.4
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    • pp.266-274
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    • 2010
  • This study aim to investigate fundamentally the growth and physiological responses of tomato plants in responses to two different levels of water deficit, a weak drought stress (-25 kPa) and a severe drought stress (-100 kPa) in soil. The two levels of water deficit were maintained using a micro-irrigation system consisted of soil sensors for the real-time monitoring of soil water content and irrigation modules in a greenhouse experiment. Soil water contents were fluctuated throughout the 30 days treatment period but differed between the two treatments with the average -47 kPa in -25 kPa set treatment and the -119 kPa in -100 kPa set treatment. There were significant differences in plant height between the two different soil water statuses in plant height without differences of the number of nodes. The plants grown in the severe water-deficit treatment had greater accumulation of biomass than the plants in the weak water-deficit treatment. The severe water-deficit treatment (-119 kPa) also induced greater leaf area and leaf dry weight of the plants than the weak water-deficit treatment did, even though there was no difference in leaf area per unit dry weight. These results of growth parameters tested in this study indicate that the severe drought could cause an adaptation of tomato plants to the drought stress with the enhancement of biomass and leaf expansion without changes of leaf thickness. Greater relative water content of leaves and lower osmotic potential of sap expressed from turgid leaves were recorded in the severe water deficit treatment than in the weak water deficit treatment. This finding also postulated physiological adaptation to be better water status under drought stress. The drought imposition affected significantly on photosynthesis, water use efficiency and stomatal conductance of tomato plants. The severe water-deficit treatment increased PSII activities and water use efficiency, but decreased stomatal conductance than the weak water-deficit treatment. However, there were no differences between the two treatments in total photosynthetic capacity. Finally, there were no differences in the number and biomass of fruits. These results suggested that tomato plants have an ability to make adaptation to water deficit conditions through changes in leaf morphology, osmotic potentials, and water use efficiency as well as PSII activity. These adaptation responses should be considered in the screening of drought tolerance of tomato plants.

A Study on Optimum Rate of Cattle Slurry Application for Forage Production using Idled Arable Land. I. The effect of applying time of cattle slurry and fertilization added mineral nitrogen on dry matter yield in Orchardgrass (유휴지에서 조사료 생산을 위한 적정 가축분뇨의 시용에 관한 연구 I. 액상구비의 시용시기와 무기태 질소의 첨가가 Orchardgrass의 건물수량에 미치는 영향)

  • Jo, Ik Hwan
    • Journal of the Korea Organic Resources Recycling Association
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    • v.2 no.2
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    • pp.65-75
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    • 1994
  • The aim of this experiment was to investigate the effects of applying time of cattle slurry and fertilizing added mineral nitrogen on dry matter yield of orchardgrass and also to estimate proper levels of fertilizing nitrogen when idled arable land rapidly increased these days, which was used for the production of Orchardgrass. The results were as follows. 1. The annual yields of dry matter were produced 5.62~6.67 ton per ha when cattle slurry applied at rates of $30m^3$(average mineral fertilizer equivalent to 120kg) per ha in different dressing times. Those were higher 0.60~1.65 ton or 0.16 ton per ha than the yields from non-fertilizing or phosphrous and potassium fertilizing. 2. Relative yields of annual dry matter from mineral nitrogen fertilization of 120kg per ha were 142~146% in comparsion with control plots(cattle slurry plot or phosphrous and potassium plot). On the other hand, those of cattle slurry application were 112~133% in comparion with non-fertilizing plot. 3. The efficiencies of nitrogen on dry matter yield(kg DM/kg N) were 5.0~13.8 DM kg/N kg when cattle slurry applied to Orchardgrass at rates of $30m^3$(average mineral fertilizer equivalent to 120kg) per ha, and were 21.3~23.1kg DM/kg N at the same level fertilization of mineral nitrogen. 4. On the condition of this study, mineral nitrogen were fertilized after applying cattle slurry at rates of $30m^3$(average mineral fertilizer equivalent to 120kg) per ha in order to minimize chemical fertilizer and increase the potential production of Orchardgrass. At this situation, marginal dry matter yields of 8.48~8.63 ton per ha were obtained as the level of economic even point goint when mineral nitrogen fertilization is between 119.4 and 133.3kg per ha. It was possible to obtain the maximal dry matter yield(8.98 tons/ha) with fertilization of 202.8kg N/ha.

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In vivo assessment of the nutritional value of Allomyrina dichotoma larva protein as future protein resource (미래 식량자원으로써 장수풍뎅이 유충의 영양학적 평가)

  • Choi, Young-Jin;Fan, Meiqi;Kim, Eun-Kyung;Lee, Seul-bi;Hwang, Yeon-hyeon;Jang, Yeong-Ho;Bae, Sung-Mun
    • Korean Journal of Food Science and Technology
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    • v.52 no.6
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    • pp.610-615
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    • 2020
  • Edible insects might be used as a means to solve food insecurity caused by population growth. Many studies have investigated the biological activity of insects; however, few studies have investigated the nutritional value of insects. Therefore, the aim of this study was to investigate the nutritional value of Allomyrina dichotoma larva protein (ADP) as a source of protein replacement. In vivo studies were conducted to determine the food efficiency ratio (FER), protein efficiency ratio (PER), and true digestibility (TD) of ADP. Experiments were conducted in 3 groups of 8 animals per group using twenty-four 4-week-old SD rats. The experimental groups included the general diet group (Con), in which 20% of the total Kcal in the diet was composed of casein protein, and the ADP group (ADP), in which 20% of the total Kcal was composed of ADP protein, and a non-protein diet group (NP) to measure the protein (metabolic fecal nitrogen) excreted by metabolic processes in the body. As a result of this experiment, we found that the FERs were 0.52 and 0.41 in the casein protein intake (Con) and ADP groups, respectively, thus showing a significantly lower level in the ADP group. The PERs of ADP and Con were 2.39 and 2.63, respectively. The TD of Con and ADP were 91% and 80%, respectively.

Effects of Composted Pig Manure on Rice Cultivation in Paddy Soils of Different Texture (논토양검정에 의한 토성별 돈분퇴비 적정 시용량 결정)

  • Song, Yo-Sung;Kwak, Han-Kang;Hyun, Byung-Keun;Yeon, Byeong-Yeol;Kim, Pil-Joo
    • Korean Journal of Soil Science and Fertilizer
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    • v.34 no.4
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    • pp.265-272
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    • 2001
  • A manure compost has been identified as an alternative to fertilizer to increase soil fertility and crop production in farming fields. The aim of the present study was to evaluate the effects of pig manure compost on soil properties and rice productivity as well as to determine the optimum application rate. In 1997, a field experiment was carried out to evaluate the growth of rice on sandy loam, loam, and clay loam soils amended with 0, 5, 10, and $20Mg\;ha^{-1}$ of pig manure compost plus NPK, which decided by soil testing. Rice yields were higher in soils receiving manure compost amendment. The maxim um yields were evaluated with $7,520kg\;ha^{-1}$ in the levels of $4.2Mg\;ha^{-1}$ of pig manure compost application plus NPK in sandy loam, $7,320kg\;ha^{-1}$ in the levels of $10.7Mg\;ha^{-1}$ in loam, and $6,320kg\;ha^{-1}$ in $17.2Mg\;ha^{-1}$ in clay loam soil. The optimum application rate of pig manure compost, which decided for 95% of maximum yields, was $4.0Mg\;ha^{-1}$ in sandy loam and $7.0Mg\;ha^{-1}$ in loam and clay loam soils under the condition of chemical fertilization by soil diagnosis. An increase in rice yield indicated a better nutrient status in compost-amended soil which was supported by the higher nutrient contents of N, P and K in shoot of plants grown in soil with manure compost amendment. Addition of manure compost increased available phosphate, silicate and exchangeable K in the amended soils according to the rate of compost application rate. It can be concluded that the manure compost could be a suitable organic fertilizer for improving rice productivity and soil fertility, and an application rate of $4.0Mg\;ha^{-1}$ in sandy loam and $7.0Mg\;ha^{-1}$ in loam and clay loam soils would give the optimum rice yields in the standard fertilization by chemical fertilization.

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Sensory and Physical Characteristics of Hwajeun Depended on the Various Levels of Oil Amounts and frying Time (기름의 양과 지지는 시간에 따른 화전의 관능적 및 물리적 특성)

  • 이승현;박정은;장명숙
    • Korean journal of food and cookery science
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    • v.19 no.6
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    • pp.765-771
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    • 2003
  • The aim of this study was to standardize the various methods and recipes of Hwajeun (glutinous rice pancake with flower) appeared in the literature. For this purpose, the effects on the sensory and rheological characteristics of Hwajeun in terms of the various levels of oil and frying times were investigated. This experiment consisted of a sensory evaluation in terms of the acceptance (color, flavor, oily feeling, softness, chewiness, adhesiveness, taste and overall preference) and objective evaluation performed by a texture analyzer, a color difference meter and measurements of the water content and oil absorption. The moisture content of the treatments as a function of the frying time, S7, showed a significantly(p<0.01) low value, and the oil absorption of the Hwajeun made with different frying times increased in proportion to the increment of the frying time(p<0.001). In terms of color, the b value of the Hwajeun made with 16g oil for frying showed a higher value than the others. In the two bite compression test, the hardness of the Hwajeun made with 4g oil for frying showed a significantly (p<0.001) high value among the S2 and S3 samples. As a result of the sensory evaluation for the Hwajeun made with various amounts of oil and frying times, the Hwajeun made with 16g oil for frying and a 2 min frying time for each side (S6) was the most preferred in terms of the softness, chewiness, taste and overall preference of the sensory evaluation

Effect of Topical Application of Intercellular Lipids on Sodium Lauryl Sulphate-Damaged Skin Barrier Function in Dogs (개에서 sodium lauryl sulphate에 의한 손상 피부에 대한 각질세포간 지질의 국소적용 효과)

  • Hwang, Sun-Jin;Oh, Won-Seok;Ku, Sae-Kwang;Lee, Keun-Woo;Oh, Tae-Ho
    • Journal of Veterinary Clinics
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    • v.25 no.5
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    • pp.330-339
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    • 2008
  • Ceramide, cholesterol and free fatty acids are the major intercellular lipids, maintaining the integrity of the skin barrier. However, the roles of these lipids in canine skin barrier function are little known. The aim of this study was to evaluate the repairing effects of 2% ceramide (CER), 2% cholesterol (CHO), 2% linoleic acid (LIN) and 2% intercellular lipid mixture (ILM) on damaged canine skin barrier by 1.25% sodium lauryl sulphate (SLS). Transepidermal water loss (TEWL), skin hydration, skin pH and skin thickness were assessed. Histological profiles and transmission electron microscopic (TEM) profiles were assessed on day 12. SLS effectively induced the canine skin barrier damage. TEWL was significantly decreased by topical application of CER and ILM in SLS and vehicle-treated skin on day 8 and 12, respectively (p < 0.05, p < 0.0 I). By end of the experiment all lipids significantly decreased the TEWL as compared with SLS and vehicle control, but CER and ILM more significantly decreased the TEWL than UN and CHO, respectively (p < 0.01). Skin hydration was significantly increased by CER and ILM during experimental periods (p < 0.01). Skin pH was significantly decreased by CER, LIN and ILM. In histological profiles, the thickness of the stratum corneum (SC) was significantly increased by the SC lipids as compared with vehicle and SLS (p < 0.01). Especially, CER and ILM showed more prominent improvement of barrier recovery. In TEM of the SC, SLS induced exfoliations of corneodesmosomes in the SC, and CER and ILM effectively protected exfoliations of corneodesmosomes on SLS-damaged canine skin. These results indicated that topical application of CER and ILM dramatically improved damaged-skin barrier function by SLS. Also, it was considered that the use of CER or ILM was recommended for the management of skin barrier dysfunction by irritant and inflammatory skin disorders such as atopic dermatitis.

A Study on the Development and Physical Properties of Low Yellowing Epoxy for Ceramic Preservation (도자기 복원용 저황변 Epoxy 수지의 개발 및 물성에 대한 연구)

  • Kim, Woo Hyun;Cheong, Da Som;Bae, Jin Soo;Jee, Joo Yeon;Wi, Koang Chul
    • Journal of Conservation Science
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    • v.30 no.2
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    • pp.181-187
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    • 2014
  • AThe studies on ceramic preservation have been conducted widely in various institutions such as national/ public museums and research labs as well as the excavation institutions and university museums. Although there are some differences in preservation methodologies and materials used across the institutions, the variation is minimal. Specifically, epoxy resin is mostly used for ceramic restoration for its high cohesiveness, low contraction and high strength although there are some variations in for Ceramic Preservation. The synthetic resin type used according to the type of damage in the ceramic. However, the yellowing or the change of color across the time after the restoration is the weakness of epoxy resin. In this study, we aim to develop a material which minimizes this yellowing of epoxy resin while enhancing its cohesiveness and strength as well as other physical properties. We made the new material to have similar properties with those used widely for the ceramic restoration, such as EPO-TEK301$^{(R)}$, L30$^{(R)}$, XTR-311$^{(R)}$ through comparative experiments. The cohesiveness of the newly developed resin was improved to 2.51(MPa), which is similar level of XTR-311$^{(R)}$ of the 2.30(MPa) but about 2x higher than the other resins EPO-TEK301$^{(R)}$, L30$^{(R)}$ (1.21 and 1.81 (MPa), respectively). Especially, the experiment on yellowing shows that the existing resins show the range of color change at 10~25(${\Delta}E^*ab$), but the new low yellowing epoxy resin has the color change value at 8.3 (${\Delta}E^*ab$), the value lowering the yellowing effect to 1 to 3 times of the existing epoxy resin, thereby solving the issue of generating sense of differences due to change of color or yellowing.

Calibration of crop growth model CERES-MAIZE with yield trial data (지역적응 시험 자료를 활용한 옥수수 작물모형 CERES-MAIZE의 품종모수 추정시의 문제점)

  • Kim, Junhwan;Sang, Wangyu;Shin, Pyeong;Cho, Hyeounsuk;Seo, Myungchul
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.20 no.4
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    • pp.277-283
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    • 2018
  • The crop growth model has been widely used for climate change impact assessment. Crop growth model require genetic coefficients for simulating growth and yield. In order to determine the genetic coefficients, regional growth monitoring data or yield trial data of crops has been used to calibrate crop growth model. The aim of this study is to verify that yield trial data of corn is appropriate to calibrate genetic coefficients of CERES-MAIZE. Field experiment sites were Suwon, Jinju, Daegu and Changwon. The distance from the weather station to the experimental field were from 1.3km to 27km. Genetic coefficients calibrated by yield trial data showed good performance in silking day. The genetic coefficients associated with silking are determined only by temperature. In CERES-MAIZE model, precipitation or irrigation does not have a significant effect on phenology related genetic coefficients. Although the effective distance of the temperature could vary depending on the terrain, reliable genetic coefficients were obtained in this study even when a weather observation site was within a maximum of 27 km. Therefore, it is possible to estimate the genetic coefficients by yield trial data in study area. However, the yield-related genetic coefficients did not show good results. These results were caused by simulating the water stress without accurate information on irrigation or rainfall. The yield trial reports have not had accurate information on irrigation timing and volume. In order to obtain significant precipitation data, the distance between experimental field and weather station should be closer to that of the temperature measurement. However, the experimental fields in this study was not close enough to the weather station. Therefore, When determining the genetic coefficients of regional corn yield trial data, it may be appropriate to calibrate only genetic coefficients related to phenology.

Expression of nitric oxide synthases in the mandibular condyle of anterior repositioned rat mandibles (백서의 하악골 전방 재위치 시 하악과두 조직에서의 nitric oxide synthases 발현 양상)

  • Kim, Hyun-Sook;Kim, Ho-Young;Heo, Sung-Su;Kang, Kyang-Hwa;Kim, Sang-Cheol
    • The korean journal of orthodontics
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    • v.40 no.4
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    • pp.239-249
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    • 2010
  • Objective: The aim of this study was to identify the expression of nitric oxide synthases (NOS) in the mandibular condyle during mandible advancement by functional appliance and to correlate it with the histologic changes and bone remodeling. Methods: Twenty-four female, 35-day-old Sprague-Dawley rats were randomly divided into 3 experimental groups. In all experimental groups, the mandibles of the rats were kept in a continuous forward position with a fixed bite jumping appliance. The rats were sacrificed on the 3rd, 14th, and 30th days of experiment. More than 2 rats in each group were used for staining. Results: There were no remarkable histologic changes and NOS expression differences in the control group. The most prominent histologic changes occurred in the 14th day experimental group. NOS decreased in the 30th day experimental group. There was increased expression of $NOS_2$ and $NOS_3$ in all experimental groups, comparative to the control group. In all the experimental groups and control group, the expression of $NOS_2$ was greater than that of $NOS_3$. Conclusions: It is postulated that $NOS_2$ and $NOS_3$ in the mandibular condyle might play an important role in bone remodelling of the mandibular condyle.