• 농촌지도와개발
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• 제25권4호
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• pp.175-184
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• 2018

There are two main types of agricultural extension projects in Korea, the impacts and routes of each type on farm income are different. This paper empirically studies the impact of agricultural extension to farms' income, using Multi-level production function considering time lag. It is found that direct type of extension has positive effect to farms' income. Also indirect type on income is significant only when the level of education is high. Due to the characteristics of Korean agricultural structure, the technical level of farm is greatly influenced by the government's R&D investment and technology guidance. The result implies that indirect type of extension that take into account the educational level of farms should be emphasized for long-term technological advances.

• 농촌지도와개발
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• 제20권3호
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• pp.679-700
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• 2013

최근 농촌지역이 쇠퇴하면서 농촌사회의 중심공간이었던 전통정기시장이 점차 축소되고 상권이 붕괴되면서 존폐를 위협하고 있는 실정이다. 다양한 분야에 걸쳐 전통정기시장의 활성화를 위한 대안을 제시하고 있지만 사회적, 지리적 관점에서 이루어진 연구는 정량적으로 분석하는데 한계가 있었다. 이에 본 연구에서는 강원도의 대표적인 5일장이었던 평창 대화장을 대상으로 정량적인 분석방법인 공간구문론을 활용하여 공간구조 및 보행동선을 분석하였다. 분석결과, 전체적인 공간구조는 통합도와 명료도가 높게 나타나 방문객이 전체 공간을 인지하고 이동하기에 효율적인 구조이지만 소비를 촉진시키는 전략이 필요할 것으로 판단되었다. 또한 보행동선을 분석한 결과 시장의 중심부에 위치하거나 주요시설 주변에 분포하는 동선의 통합도가 높게 나타났다. 자연발생적으로 생겨난 전통정기시장은 단순하고 명료한 공간구조를 갖고 있다는 점에서 문제점이 있으며, 쇠퇴한 정기시장을 활성화하기 위해서는 장소성을 강화하고 방문객의 소비를 촉진하는 전략이 필요할 것으로 사료된다.

• Molecules and Cells
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• 제38권2호
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• pp.180-186
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• 2015

Escherichia coli AcrAB-TolC is a multidrug efflux pump that expels a wide range of toxic substrates. The dynamic nature of the binding or low affinity between the components has impeded elucidation of how the three components assemble in the functional state. Here, we created fusion proteins composed of AcrB, a transmembrane linker, and two copies of AcrA. The fusion protein exhibited acridine pumping activity, suggesting that the protein reflects the functional structure in vivo. To discern the assembling mode with TolC, the AcrBA fusion protein was incubated with TolC or a chimeric protein containing the TolC aperture tip region. Three-dimensional structures of the complex proteins were determined through transmission electron microscopy. The overall structure exemplifies the adaptor bridging model, wherein the funnel-like AcrA hexamer forms an intermeshing cogwheel interaction with the ${\alpha}$-barrel tip region of TolC, and a direct interaction between AcrB and TolC is not allowed. These observations provide a structural blueprint for understanding multidrug resistance in pathogenic Gram-negative bacteria.

• 한국농공학회논문집
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• 제64권5호
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• pp.67-77
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• 2022

Recently, the trend in structural analysis and design is moving towards the development of reliable system. The reliability-based method defines various limit states related to usability and failure, thereby enabling multiple levels of design according to the importance of a structure. Meanwhile, an actual structure is composed of a set of several elements, and particularly, a frame type is composed of a system in which the members are connected each other. At this time, the actual connection between members is in a semi-rigid condition, not in complete rigid or hinged. This semi-rigid is found in several structures, especially in agricultural facilities designed with lightweight materials. In this study, a system reliability analysis technique for frame structure was established, and applied to an analysis of the semi-rigid connection. Various conditions of correlation were applied to reflect the connectivity between members, and through this, the limitations of existing structural analysis method and the behavioral characteristics of structure were analyzed. The failure probability of the frame member component and the overall structure system was significantly different in consideration of the semi-rigid connection. In addition, it was evaluated that the behavior of structure can be more accurately analyzed if the correlation according to the position of members in a system is further investigated.

• Molecules and Cells
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• 제40권4호
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• pp.299-306
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• 2017

The transcriptional activator AphB has been implicated in acid resistance and pathogenesis in the food borne pathogens Vibrio vulnificus and Vibrio cholerae. To date, the full-length AphB crystal structure of V. cholerae has been determined and characterized by a tetrameric assembly of AphB consisting of a DNA binding domain and a regulatory domain (RD). Although acidic pH and low oxygen tension might be involved in the activation of AphB, it remains unknown which ligand or stimulus activates AphB at the molecular level. In this study, we determine the crystal structure of the AphB RD from V. vulnificus under aerobic conditions without modification at the conserved cysteine residue of the RD, even in the presence of the oxidizing agent cumene hydroperoxide. A cysteine to serine amino acid residue mutant RD protein further confirmed that the cysteine residue is not involved in sensing oxidative stress in vitro. Interestingly, an unidentified small molecule was observed in the inter-subdomain cavity in the RD when the crystal was incubated with cumene hydroperoxide molecules, suggesting a new ligand-binding site. In addition, we confirmed the role of AphB in acid tolerance by observing an aphB-dependent increase in cadC transcript level when V. vulnificus was exposed to acidic pH. Our study contributes to the understanding of the AphB molecular mechanism in the process of recognizing the host environment.

• Molecules and Cells
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• 제38권8호
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• pp.715-722
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• 2015

In Gram-negative bacteria in the periplasmic space, the dimeric thioredoxin-fold protein DsbC isomerizes and reduces incorrect disulfide bonds of unfolded proteins, while the monomeric thioredoxin-fold protein DsbA introduces disulfide bonds in folding proteins. In the Gram-negative bacteria Salmonella enterica serovar Typhimurium, the reduced form of CueP scavenges the production of hydroxyl radicals in the copper-mediated Fenton reaction, and DsbC is responsible for keeping CueP in the reduced, active form. Some DsbA proteins fulfill the functions of DsbCs, which are not present in Gram-positive bacteria. In this study, we identified a DsbA homologous protein (CdDsbA) in the Corynebacterium diphtheriae genome and determined its crystal structure in the reduced condition at $1.5{\AA}$ resolution. CdDsbA consists of a monomeric thioredoxin-like fold with an inserted helical domain and unique N-terminal extended region. We confirmed that CdDsbA has disulfide bond somerase/reductase activity, and we present evidence that the N-terminal extended region is not required for this activity and folding of the core DsbA-like domain. Furthermore, we found that CdDsbA could reduce CueP from C. diphtheriae.

• Food Science and Biotechnology
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• 제17권4호
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• pp.705-712
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• 2008

Sweet potato starch was modified using Thermus aquaticus $\alpha$-1,4-glucanotransferase ($Ta{\alpha}GT$), and its structural and rheological properties were investigated. $Ta{\alpha}GT$-modified starch had a lower amylose level and molecular weight than raw starch. The chain length distribution showed an increased number of short and long branched chains and the formation of cycloamyloses. Compared with raw starch, $Ta{\alpha}GT$-modified starch displayed a lower gelatinization enthalpy and a wider melting temperature range. The X-ray diffraction of $Ta{\alpha}GT$-modified starch was a weak V-type pattern with distinct sharp peaks at 13 and $20^{\circ}$. Scanning electron micrographs of modified starch exhibited big holes on the surface and the loss of granular structure. The frequency sweep measurement revealed that the gel of $Ta{\alpha}GT$-modified starch was more rigid than raw starch gel. However, the structure of modified starch gel was destroyed by heating at $75^{\circ}C$, and a firm gel was re-formed by subsequent storage at $5^{\circ}C$, indicating thermoreversible property.

• Animal Bioscience
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• 제35권12호
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• pp.1839-1849
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• 2022

Objective: The study aims to uncover the genetic diversity and unique genetic structure of the Min pig conserved population, divide the nucleus conservation population, and construct the molecular pedigree. Methods: We used KPS Porcine Breeding Chip v1 50K for SNP detection of 94 samples (31♂, 63♀) in the Min pig conserved population from Lanxi breeding Farm. Results: The polymorphic marker ratio (PN), the observed heterozygosity (Ho), and the expected heterozygosity (He) were 0.663, 0.335, and 0.330, respectively. The pedigree-based inbreeding coefficients (F_PED) was significantly different from those estimated from runs of homozygosity (F_ROH) and single nucleotide polymorphism (F_SNP) based on genome. The Pearson correlation coefficient between F_ROH and F_SNP was significant (p<0.05). The effective population content (Ne) showed a continuously decreasing trend. The rate of decline was the slowest from 200 to 50 generations ago (r = 0.95), then accelerated slightly from 50 to 5 generations ago (1.40

• 농업과학연구
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• 제49권4호
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• pp.823-835
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• 2022

In this study, the feasibility of alternative tractor Roll Over Protective Structure (ROPS) designed to evaluate conditions required for testing was confirmed. In accordance with Organization for Economic Cooperation and Development (OECD) code 4, the required load energy of the tractor ROPS was determined. First, the tractor ROPS test was performed and a repeated test was performed using a simplified ROPS as an alternative tractor ROPS. The test procedure is first rearward, second lateral, and last forward based on ROPS. The load test device consists of a load cell that measures force and a LVDT that measures deformation. Precision was confirmed by calculating the relative standard deviation of the simplified ROPS repeated test. Accuracy was analyzed by calculating the mean relative error between the mean measured values in the simplified ROPS test and the tractor ROPS test. As a result, the relative standard deviation was less than 2.5% for force and 3.3% for maximum deformation overall, showed the highest precision in lateral load. The mean relative error value for force measured at the lateral load of simplified ROPS was 0.5%, showing the highest accuracy. In the front load test, the mean relative error of maximum deformation was 20.5%, showing the lowest accuracy. The mean relative error (MRE) was high in the forward load test was because of structural factors of the ROPS. The simplified ROPS model is expected to save money and time spent preparing tractors.

• International Journal of Industrial Entomology and Biomaterials
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• 제19권2호
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• pp.255-258
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• 2009

Structure and Properties of sericinjam sericin extracted was investigated using SDS-PAGE electrophoresis, UV, CD, and DSC. The molecular weight and its distribution of sericinjam sericin showed broad tailing pattern. Circular dichlroism spectra showed that the structure of sericinjam sericin in solution was different with domestic one. However, differential thermal calorimetric properties are similar to each other.