• Journal of Microbiology and Biotechnology
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• 제18권2호
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• pp.234-241
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• 2008

Agrobacterum tumefaciens-mediated transformation (ATMT) is becoming an effective system as an insertional mutagenesis tool in filamentous fungi. We developed and optimized ATMT for two Colletotrichum species, C. falcatum and C. acutatum, which are the causal agents of sugarcane red rot and pepper anthracnose, respectively. A. tumefaciens strain SK1044, carrying a hygromycin phosphotransferase gene (hph) and a green fluorescent protein (GFP) gene, was used to transform the conidia of these two Colletotrichum species. Transformation efficiency was correlated with co-cultivation time and bacterial cell concentration and was higher in C. falcatum than in C. acutatum. Southern blot analysis indicated that about 65% of the transformants had a single copy of the T-DNA in both C. falcatum and C. acutatum and that T-DNA integrated randomly in both fungal genomes. T-DNA insertions were identified in transformants through thermal asymmetrical interlaced PCR (TAIL-PCR) followed by sequencing. Our results suggested that ATMT can be used as a molecular tool to identify and characterize pathogenicity-related genes in these two economically important Colletotrichum species.

• The Korean Journal of Community Living Science
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• 제27권spc호
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• pp.689-702
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• 2016

In this study, it was promoted for the purpose of direction for rural landscape planning and management through the analysis of the factors of change in the rural landscape. In the course of research, through literature review study and field surveys, to derive the 25 factors that influence the change in the rural landscape. Later, 25 factors were evaluated in the impact on landscape by experts. 25 factors of the rural landscape were a comprehensive analysis and the results of literature review study and field surveys about the five villages of research area; Ji-San village, Non-Gae village, etc. Expert evaluation was carried out by the rural landscape, and architecture professionals of various related fields. As a result of the analysis, the flow of rural development policy and the business had a great effect on the physical changes in the rural landscape. Furthermore, additional factors such as population structure and lifestyle have made the change in the complex landscapes. Meaning the study has is to provide information on what need there are considered factors in the rural landscape planning and management. The reason is that, since the rural landscape characteristics are different, in order to develop the more efficient rural landscape plan and management.

• Journal of Korean Society of Rural Planning
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• 제24권1호
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• pp.1-10
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• 2018

This study is to evaluate the per capita accessibility to child care facilities using road map in rural village unit considering the supply and demand of child care facilities in municipal (Si-Gun) units. Using these estimated accessibility, the most accessible regions to child care facilities was identifies using Moran's index. Assuming establish a new child care facility in the most accessible region, the sensitivity of child care environment was analyzed. The number of regions are 71 si-gun-gu where supply of child care facilities is insufficient. The average accessibility per capita is 1.09 km to child care facilities and the average accessibility in Myeon unit is approximately 2.2 times higher than accessibility in Eup unit (Eup unit 0.54 km, Myeon unit 1.21 km). Approach tendency from village to child care facilities has positive relationship as 0.451 global Moran's index. The high-high (H-H) accessibility regions are wide as Gangwon-do, Gyeongsangbuk-do, Gyeongsangnam-do, Jeollanam-do and Chungcheongbuk-do. Assumed to be established the new child care facilities in Yangyang-gun (Ganwon-do), accessibility changes of child care environment are up to 2.7 times greater and the recipient population is 77% of Yangyang-gun.

• Journal of The Korean Society of Agricultural Engineers
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• 제61권5호
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• pp.57-68
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• 2019

Rural tourism has been identified as providing opportunities to revitalize the rural economies since early 2000s. During last decade in South Korea, government has enacted many policies aiming to establish tourism support programs as well as instigating activities between rural and nearby urban districts. In particular, the promotion of the rural tourism has been actively sought in various forms of products and services, such as green tourism village, village festival, one-company one-village campaign and etc. However, in order to prepare effective policies, it is very crucial to identify measureable evaluation criteria that can be used to assess socio-economic situations of the rural districts. Such criteria should be objective, timely and provide insights about living conditions of local residents and relevant properties. They can also be used to evaluate if progress is being made after the policies are implemented. While considerable efforts and resources have been invested to aid the tourism programs in South Korea, we still lack such systematic means to quantify and evaluate its impact on rural districts. In this work, we have applied regional deprivation analysis on well-established community experiential and recreational business in rural villages throughout the country based on three criteria (i.e., population, local commerce, and buildings). Surprisingly, the result shows that the implementation of the community experiential and recreational businesses did not bring any noticeable changes to at least one of the evaluation criteria. We concluded that the current government policies on revitalization of rural tourism at the village scale is insufficient for bringing meaningful socio-economic impacts to rural districts, with rare exceptions.

• Korean Journal of Plant Resources
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• 제23권6호
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• pp.550-553
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• 2010

The representative research institutes in North Korea are the Academy of Science and the laboratories of major universities. The National Academy of Science consists of headquarters, Academy of Medicinal Science, Academy of Forestry Science, and Academy of Agricultural Science. Under the authority of the National Academy of Science, Central Information Agency for Science and Technology (CIAST) has built up a database system integrating all the science technology data to provide scientific and technological information. The major universities of North Korea include Kim Il-sung University, Kim Chaek University of Technology, Pyongyang Agricultural College, and Wonsan Agricultural College, and an agricultural college is situated in each province. Out of 3,860 plant species in North Korea, 158 species which amounts to approximately 4% of the total are rare and endangered, and deforestation is under a critical situation. The Oriental Medicine (Koryo Medicine) has been well developed using medicinal plants, and practical researches on biotic pesticide are largely conducted as an alternative to tackle the shortage of farming materials. Hereafter, a South-North joint research on the amount of plant resources and the methods of its conservation is needed and the North Korean research areas of biotic pesticide are worth adopting to the South Korean eco-friendly organic agriculture.

• Proceedings of the Plant Resources Society of Korea Conference
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• 한국자원식물학회 2005년도 춘계 학술발표대회
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• pp.186-186
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• 2005

• Proceedings of the Plant Resources Society of Korea Conference
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• 한국자원식물학회 2005년도 춘계 학술발표대회
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• pp.117-117
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• 2005

• Proceedings of the Plant Resources Society of Korea Conference
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• 한국자원식물학회 2002년도 제9차 국제심포지움 및 추계정기학술발표회
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• pp.28-28
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• 2002

• Proceedings of the Plant Resources Society of Korea Conference
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• 한국자원식물학회 2007년도 추계학술발표회
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• pp.327.2-327.2
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• 2007

• Proceedings of the Plant Resources Society of Korea Conference
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• 한국자원식물학회 2007년도 추계학술발표회
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• pp.328.1-328.1
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• 2007