• 한국육종학회지
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• 제40권3호
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• pp.211-214
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• 2008

Haploid system by anther culture allows the development of homozygous lines when doubled. The response of anther culture to Basta (glufosinate) resistance was investigated on transgenic plants (cv. Anjungbyeo) in order to identify inheritance of bar gene associated with Basta. Most of the regenerated transgenic plants were sterile, and only a few plants produced viable seeds ($A_1$) in the greenhouse. The bar gene was analysis by PCR in basta resistant transgenic plant ($TA_0$). The transgenic seeds ($A_1$) were significantly germinated in Basta solution compared with non-transformed seeds. As a result of anther culture, in regenerated haploid plants, segregation ratio was 1:1 in five of eight cross combinations. In diploid plants, segregation ratio was 1:1 in seven of eight cross combinations. Although there was some differences in the cross combinations, most of the combinations had 1:1 segregation ratio which supports the theory. The difference may be a result of the small sample size or the difference of anther culture response caused by genotypic difference. Hence, when many cross combinations were anther-cultured the results would support the theory.

• 농업과학연구
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• 제48권1호
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• pp.179-189
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• 2021

Plants need essential mineral elements to favorably develop and to complete their life cycle. Despite the irreplaceable roles of microelements, they are often ignored due to the relative importance of macroelements with their influence on crop growth and development. We focused on the changes in primary metabolites in the leaves and roots of bell pepper plants under 6 microelements-deficient conditions: Copper (Cu), Zinc (Zn), Iron (Fe), Manganese (Mn), Boron (B) and Molybdenum (Mo). Bell pepper plants were grown in hydroponic containers, and individual elements were adjusted to 1/10-strength of Hoagland nutrient solution. A remarkable perturbation in the abundance of the primary metabolites was observed for the Fe and B and the Mn and B deficiencies in the leaves and roots, respectively. The metabolites with up-accumulation in the Fe-deficient leaves were glucose, fructose, xylose, glutamine, asparagine and serine. In contrast, the Mn deficiency also resulted in a higher accumulation of glucose, fructose, xylose, galactose, serine, glycine, β-alanine, alanine and valine in the roots. The B deficiency noticeably accumulated alanine, valine and phenylalanine in the roots while it showed a substantial decrease in glucose, fructose and xylose. These results show that the primary metabolism could be seriously disturbed due to a microelement deficiency, and the alteration may be either the specific or adaptive responses of bell pepper plants.

• 환경생물
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• 제41권4호
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• pp.539-549
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• 2023

This study examined the impact of two bacterial strains, H05E-12 and H05R-04, on alleviating non-irrigation-induced stress and its subsequent effects on the fruit productivity of sweet pumpkin plants. When subjected to non-irrigation-induced stress, the lipid peroxidation, proline, total phenol, and total soluble sugar content significantly decreased in plants treated with either H05E-12 or H05R-04 compared to the control. In a greenhouse experiment under non-irrigated conditions, H05E-12-treated plants exhibited higher stomatal conductance than the control, although there was no significant change in the soil plant analysis development(SPAD) value due to treatment. Upon re-watering, an increase in fruit diameter was observed in H05E-12-treated plants, and the L-ascorbic acid content in the fruit also showed a significant increase compared to the control. The H05E-12 strain was identified as Kushneria konosiri. To the best of our knowledge, this is the first report detailing the beneficial effects of K. konosiri on the alleviation of non-irrigation-induced stress and the promotion of plant growth in sweet pumpkin plants.

• 한국환경복원기술학회지
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• 제20권1호
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• pp.35-53
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• 2017

This study analyzed the relationship between butterflies and plants. We examined 8 butterfly greenhouse. Butterflies are 5 families, 22 species found in 8 locations. Papilio xuthus, Pieris rapae, Papilio bianor etc. was expected to be introduced in the garden. Plants were identified in the 8 greenhouse with a total 249 taxa to 82 families 186 gunus 224 species 23 variety 2 forma. The main planting species were Compositae, Liliaceae, Rosaceae, Umbelliferae, Crassulaceae, Rutaceae and Etc. A main life forms are Hemicryptophytes. The naturalized plants have been identified 7 families 11 species. And planting in the garden, we propose appropriate management. Host plant is confirmed Rutaceae, Umbelliferae Leguminosae, Cruciferae, Ulmaceae, Aristolochiaceae Etc.. Main nectar plant is Compositae, Liliaceae, Rosaceae, Crassulaceae, Labiatae. Nectar plant is proposed to be planted in consideration of the flowering period. Zanthoxylum piperitum, Zanthoxylum schinifolium, Phellodendron amurense, Poncirus trifoliata, Citrus junos, Ruta graveolens proposed design reflects the woody. Spiraea(Rosaceae) and Rhododendron(Ericaceae) proposed by Nectar plants of woody. We hope to be utilized in the planning and construction of a butterfly garden.

• Journal of Microbiology and Biotechnology
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• 제22권3호
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• pp.301-310
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• 2012

Prosopis juliflora and Parthenium hysterophorus are the two arid, exotic weeds of India that are characterized by distinct, profuse growth even in nutritionally poor soils and environmentally stressed conditions. Owing to the exceptional growth nature of these two plants, they are believed to harbor some novel bacterial communities with wide adaptability in their rhizosphere. Hence, in the present study, the bacterial communities associated with the rhizosphere of Prosopis and Parthenium were characterized by clonal 16S rRNA gene sequence analysis. The culturable microbial counts in the rhizosphere of these two plants were higher than bulk soils, possibly influenced by the root exudates of these two plants. The phylogenetic analysis of V1_V2 domains of the 16S rRNA gene indicated a wider range of bacterial communities present in the rhizosphere of these two plants than in bulk soils and the predominant genera included Acidobacteria, Gammaproteobacteria, and Bacteriodetes in the rhizosphere of Prosopis, and Acidobacteria, Betaproteobacteria, and Nitrospirae in the Parthenium rhizosphere. The diversity of bacterial communities was more pronounced in the Parthenium rhizosphere than in the Prosopis rhizosphere. This culture-independent bacterial analysis offered extensive possibilities of unraveling novel microbes in the rhizospheres of Prosopis and Parthenium with genes for diverse functions, which could be exploited for nutrient transformation and stress tolerance in cultivated crops.

• 한국환경농학회지
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• 제39권3호
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• pp.253-262
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• 2020

BACKGROUND: It has been reported that the nutritional composition of Locusta migratoria (Orthoptera: Acrididae) changes nutrients depending on the host plants. In this study, to confirm whether Locusta migratoria is an edible insect, the nutrients and harmful substances such as heavy metals and pathogens were analyzed and compared according to corn (LC) or wheat (LW) as host plants. METHODS AND RESULTS: All experimental methods mainly referred to AOAC (2004). The content of crude protein per dry weight was 77.3% in (LW), 1.1 times higher than 69.8% in LC. Crude fat was 6.5% in LW, 2.2 times less than LC 14.3%. Alpha-linolenic acid, which has the highest content among unsaturated fatty acids, was 1.2 times higher in LC (39.9%) than LW (32.5%). As a result of analysis of harmful substances by LC and LW, lead and cadmium among heavy metals were at levels suitable for heavy metal standards of edible insects, and arsenic was not detected in both groups. E. coli and Salmonella spp. were not detected in both groups. CONCLUSION: When comparing the overall nutrients composition of LW and LC, it was confirmed nutrients are different depending on the host plants, and the safety was proved.

• Journal of Plant Biotechnology
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• 제2권1호
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• pp.35-41
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• 2000

Transgenic cabbage (Brassica oleracea ssp. capitata) plants resistant to the commercial herbicide Bast $a^{R}$ were obtained by Agrobacterium tumefaciens - mediated transformation. Hypocotyl segments of in vitro grown plants were infected with Agrobacterium tumefaciens LBA 4404 harboring plasmid pMOG6-Bar which contains hpt and bar genes. Explants were cultured on callus induction medium (MS basal medium + 1 mg/L NAA + 2 mg/L BA + 2 mg/L AgN $O_3$+ 100 mg/L carbenicillin + 250 mg/L cefotaxime) supplemented with 15 mg/L hygromycin. Hygromycin resistant calluses were transferred to shoot regeneration medium (MS basal medium + 0.1 mg/L NAA + 2 mg/L BA + 3% sucrose + 2 mg/L AgN $O_3$+ 15 mg/L hygromycin + 250 mg/L cefotaxime + 100 mg/L carbenicillin). In order to induce roots, elongated shoots were placed on the MS medium without plant growth regulators and hygromycin. Southern blot analysis of several putative transgenic plants indicated that one to five intact copies of Apt and bar genes were incorporated into the genome. Expression of bar gene was confirmed by Northern blot analysis and by herbicide resistant phenotype. Seed progeny from self-pollinated transformants expressed the herbicide resistance and showed Mendelian segregation of the introduced gene.e.

• 농촌지도와개발
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• 제18권4호
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• pp.861-877
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• 2011

The purpose of this study is to survey job stress and Musculoskeletal Disorder(MSDs) of workers in small plants with agricultural products. The subjects were comprised of 69 workers participated in "Helping the rural women to get the small businesses" project. The questionnaire surveys were Korean Occupational Stress Scale(KOSS), and Legal Risk Assessment of Musculoskeletal Disorders(KOSHA CODE H-30-2003). As a result of the survey, scores of KOSS 8 sub-items except for job demand were similar to that of Korea workers. Prevalence of MSDs symptoms of small plants workers was high percent (44.9%). And the rate according to kinds of product was significant difference at neck (p<0.01), lumbar (p<0.01), and leg (p<0.01). There was no significant difference between view person sign of MSDs and non MSDs according to sex, year, the number of workers, working years, working time per one day, rest time per one day, and subjective working intensity, but there were significant difference between high stress group and low stress group about MSDs at interpersonal conflict(p<0.05). Job stress and MSDs were important factor to workers of small plants producted agricultural products. Therefore, starting a business with small plats, workers consider interpersonal conflict and body part showing MSDs.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1996년도 식물학심포지움 식물호르몬과 신호전달
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• pp.50-63
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• 1996

Nitrogen (N) is an important regulator of the expression of genes involved in carbon and N assimilation pathways in plants by selectively altering the levels of proteins and/or mRNAs. These in C4 plants include genes for such as phosphoenolpyruvate carboxylase, carbonic anhydrase, and pyruvate-Pi dikinase. The C4 genes are regulated in mesophyll cells by N availability both transcriptionally and posttranscriptionally through cytokinins and glutamine as signals. The level of both the signals is up-regulated by N availability: cytokinins in roots and glutamine in leaves. The level of glutamine is controlled by the differential expression by N of glutamine synthetase and ferrdoxin-dependent glutamate synthase genes which locate in the mesophyll cells of C4 plants. The results is discussed as molecular mechanism for the greater N use efficiency of the plants as well as N partitioning is the photosynthetic cells.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.556-561
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• 2010

Lactoferrin is an 80-kDa iron-binding glycoprotein that is found in high concentrations in human milk. Human lactoferrin (hLF) has several beneficial biological activities including immune system modulation and antimicrobial activity. In the present study, we devolved a method of hLF expression through introducing the hLF gene construct into Oriza sativa cv. Nakdong using the Agrobacterium-mediated transformation system. The expression of the hLF gene under the control of the rice glutelin promoter was detected in the seeds of transgenic rice plants. Transformed rice plants were selected on media containing herbicide(DL-phosphinothricin) and the integration of hLF cDNA was confirmed by Southern blot analysis. The expression of the full length hLF protein from the grains of transgenic rice plants was verified by Western blot analysis. The lactoferrin expression levels in the transformed rice grains determined by enzyme-linked immunosorbant assay accounted for approximately 1.5% of total soluble protein. Taken together, these data indicate that rice grains expressing hLF can be directly incorporated into infant formula and baby food.