• Animal Bioscience
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• 제34권4호
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• pp.525-532
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• 2021

Objective: Old World camels are a valuable genetic resource for many countries around the world due to their adaptation to the desert environment. At present, Old World camels have encountered the challenge of unprecedented loss of genetic resources. Through our research, we would reveal the population structure and genetic variation in Old World camel populations, which provides a theoretical basis for understanding the germplasm resources and origin and evolution of different Old World camel populations. Methods: In the present study, we assessed mtDNA control region sequences of 182 individuals from Old World camels to unravel genetic diversity, phylogeography, and demographic dynamics. Results: Thirty-two haplotypes confirmed by 54 polymorphic sites were identified in the 156 sequences, which included 129 domestic and 27 wild Bactrian camels. Meanwhile, 14 haplotypes were defined by 47 polymorphic sites from 26 sequences in the dromedaries. The wild Bactrian camel population showed the lowest haplotype and nucleotide diversity, while the dromedaries investigated had the highest. The phylogenetic analysis suggests that there are several shared haplotypes in different Bactrian camel populations, and that there has been genetic introgression between domestic Bactrian camels and dromedaries. In addition, positive values of Tajima's D and Fu's Fs test demonstrated a decrease in population size and/or balancing selection in the wild Bactrian camel population. In contrast, the negative values of Tajima's D and Fu's Fs test in East Asian Bactrian camel populations explained the demographic expansion and/or positive selection. Conclusion: In summary, we report novel information regarding the genetic diversity, population structure and demographic dynamics of Old World camels. The findings obtained from the present study reveal that abundant genetic diversity occurs in domestic Bactrian camel populations and dromedaries, while there are low levels of haplotype and nucleotide diversity in the wild Bactrian camel population.

• Animal Bioscience
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• 제35권2호
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• pp.260-271
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• 2022

Objective: We aimed to investigate the effect of the differing amino acid (AA) release dynamics of two protein sources on the growth performance, nitrogen deposition, plasma biochemical parameters, and muscle synthesis and degradation of piglets when included in their diets at normal and low concentrations. Methods: Forty-eight piglets (Duroc×Landrace×Large White) with initial body weight of 7.45±0.58 kg were assigned to six groups and fed one of 6 diets. The 6 dietary treatments were arranged by 3×2 factorial with 3 protein sources and 2 dietary protein levels. They are NCAS (a normal protein content with casein), NBlend (a normal protein content with blend of casein and corn gluten meal), NCGM (a normal protein content with corn gluten meal), LCAS (a low protein content with casein), LBlend (a low protein content with blend of casein and corn gluten meal), LCGM (a low protein content with corn gluten meal). The release dynamics of AA in these diets were determined by in vitro digestion. The digestibility, utilization and biological value of nitrogen in piglets were determined by micro Kjeldahl method. Plasma insulin was measured by enzyme-linked immunosorbent assay kits. The protein expression of mediators of muscle synthesis and degradation was determined by western blotting. Results: Although the consumption of a low-protein diet supplemented with crystalline AA was associated with greater nitrogen digestion and utilization (p<0.05), the final body weight, growth performance, nitrogen deposition, and phosphorylation of ribosomal protein S6 kinase 1 and eIF4E binding protein 1 in the muscle of pigs in the low-protein diet-fed groups were lower than those of the normal-protein diet-fed groups (p<0.05) because of the absence of non-essential AA. Because of the more balanced release of AA, the casein (CAS) and Blend-fed groups showed superior growth performance, final body weight and nitrogen deposition, and lower expression of muscle ring finger 1 and muscle atrophy F-box than the CGM-fed groups (p<0.05). Conclusion: We conclude that the balanced release of AA from CAS containing diets and mixed diets could reduce muscle degradation, favor nitrogen retention, % intake and improve growth performance in pigs consuming either a normal- or low-protein diet.

• 한국농림기상학회:학술대회논문집
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• 한국농림기상학회 2012년도 학술발표논문집
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• pp.129-131
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• 2012

• 한국농림기상학회:학술대회논문집
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• 한국농림기상학회 2012년도 학술발표논문집
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• pp.59-61
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• 2012

• 농촌지도와개발
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• 제8권2호
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• pp.285-298
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• 2001

Rural youth welfare was defined to provide the preventive services as well as the protective and curative services such as education, job training, youth activity, delinquency prevention, etc. for rural youth who live in rural society, including who go out to metropolitan city for study. Based on this concept, the rural youth related facilities and problems were investigated in the area of education, culture and leisure, career, and problematic behavior. The results were identified that the efforts of government and rural related institutions were in sufficient to establish and improve the facilities for rural youth, and the gap between rural and urban youth facilities was extended increasingly, in spite of seriousness of those rural youth problems. Some of policies and programs were proposed in order to improve rural youth welfare; 1) To establish at least one activity facility in every Eup ; 2) To convert the public culture facilities such as museums, fine art museums, exhibition facilities, etc. into the culture and art facilities for rural youth; 3) To establish rural youth job information center in the agricultural technology and extension center; 4) To convert school to rural youth education and cultural center; 5) To establish the comprehensive welfare facilities for rural youth in metropolitan city.

• Journal of Animal Science and Technology
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• 제57권12호
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• pp.44.1-44.9
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• 2015

Background: Heat shock proteins play an important role in protection from stress stimuli and metabolic insults in almost all organisms. Methods: In this study, computational tools were used to deeply analyse the physicochemical characteristics and, using homology modelling, reliably predict the tertiary structure of the blunt snout bream (Ma-) Hsp70 and Hsc70 proteins. Derived three-dimensional models were then used to predict the function of the proteins. Results: Previously published predictions regarding the protein length, molecular weight, theoretical isoelectric point and total number of positive and negative residues were corroborated. Among the new findings are: the extinction coefficient (33725/33350 and 35090/34840 - Ma-Hsp70/ Ma-Hsc70, respectively), instability index (33.68/35.56 - both stable), aliphatic index (83.44/80.23 - both very stable), half-life estimates (both relatively stable), grand average of hydropathicity (-0.431/-0.473 - both hydrophilic) and amino acid composition (alanine-lysine-glycine/glycine-lysine-aspartic acid were the most abundant, no disulphide bonds, the N-terminal of both proteins was methionine). Homology modelling was performed by SWISS-MODEL program and the proposed model was evaluated as highly reliable based on PROCHECK's Ramachandran plot, ERRAT, PROVE, Verify 3D, ProQ and ProSA analyses. Conclusions: The research revealed a high structural similarity to Hsp70 and Hsc70 proteins from several taxonomically distant animal species, corroborating a remarkably high level of evolutionary conservation among the members of this protein family. Functional annotation based on structural similarity provides a reliable additional indirect evidence for a high level of functional conservation of these two genes/proteins in blunt snout bream, but it is not sensitive enough to functionally distinguish the two isoforms.

• 농촌지도와개발
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• 제20권2호
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• pp.383-405
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• 2013

본 연구의 목적은 농촌지도사의 직무별로 관련성 있는 역량을 도출하고자 하였으며 이를 위해 농촌지도사의 직무와 개별역량 간의 대응분석을 실시하였다. 분석결과 농촌지도사의 지도기획 직무는 '문제해결' 및 '아이디어 창출' 역량과 관련성이 높게 나타났으며, 생활개선 교육 직무는 '지도사업평가', '고객지향성' 및 '이해력' 역량과 관련성이 높게 나타났고, 작물기술보급 직무는 '현장지도' 및 '책임성' 역량과 관련성이 높은 것으로 분석되었다. 본 연구에서는 이러한 분석결과를 바탕으로 향후 농촌지도사들의 역량강화를 통한 직무의 효과성을 제고하기 위하여 직무별로 관련성이 높게 나타난 역량들에 대한 중점적인 교육이 실시될 필요성을 제시하였다. 또한 신규지도사의 최초 직무 배치시 개별역량의 측정 결과를 바탕으로 적정한 직무에 배치할 수 있는 기초자료로 활용하고자 하였다.

• Journal of Biosystems Engineering
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• 제26권4호
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• pp.323-330
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• 2001

The regular maintenance and repair status of major farm machinery such as power tiller, farm tractor, rice transplanter and combine harvester etc., were surveyed and analyzed for the full-time farmers in order to get basic information for promoting farm machinery productivity. The survey was carried out through 209 farmers from 9 provinces. The results of this study can be summarized as follows; 1. The regular maintenance of farm machinery was conducted at repair shop(49.5%) or dealer agency(12.0%), and also conducted by farmers at their house(34.9%). 2. For the status on the cleanness or exchange times of engine oil, fuel filter and air cleaner, most farmers conducted fewer times than the necessary times. And, 5.3%, 7.7%, and 2.9% of surveyed farmers experienced breakdowns due to the insufficient cleanness or exchange oil, fuel filer, and air cleaner, respectively. 3. Most farmers(76.1%) recognized the necessity of farm machinery training or education, and they preferred one week for the training period, simple or easy maintenance for the training level, agricultural technical center or farm machinery manufacturer for the training agency. 4. Complete recognition of operation manual and sufficient maintenance before-, during-, and after-operation for the farm machinery can minimize the breakdowns, enlarge the endurance prevent the safety accidents, and promote productivity of farm machinery. And, these can be accomplished by the thorough training or education for agricultural machinery.

• Journal of Animal Science and Technology
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• 제64권2호
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• pp.312-329
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• 2022

Feed cost is the main factor affecting the economic benefits of pig industry. Improving the feed efficiency (FE) can reduce the feed cost and improve the economic benefits of pig breeding enterprises. Liver is a complex metabolic organ which affects the distribution of nutrients and regulates the efficiency of energy conversion from nutrients to muscle or fat, thereby affecting feed efficiency. MicroRNAs (miRNAs) are small non-coding RNAs that can regulate feed efficiency through the modulation of gene expression at the post-transcriptional level. In this study, we analyzed miRNA profiling of liver tissues in High-FE and Low-FE pigs for the purpose of identifying key miRNAs related to feed efficiency. A total 212~221 annotated porcine miRNAs and 136~281 novel miRNAs were identified in the pig liver. Among them, 188 annotated miRNAs were co-expressed in High-FE and Low-FE pigs. The 14 miRNAs were significantly differentially expressed (DE) in the livers of high-FE pigs and low-FE pigs, of which 5 were downregulated and 9 were upregulated. Kyoto Encyclopedia of Genes and Genomes analysis of liver DE miRNAs in high-FE pigs and low-FE pigs indicated that the target genes of DE miRNAs were significantly enriched in insulin signaling pathway, Gonadotropin-releasing hormone signaling pathway, and mammalian target of rapamycin signaling pathway. To verify the reliability of sequencing results, 5 DE miRNAs were randomly selected for quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The qRT-PCR results of miRNAs were confirmed to be consistent with sequencing data. DE miRNA data indicated that liver-specific miRNAs synergistically acted with mRNAs to improve feed efficiency. The liver miRNAs expression analysis revealed the metabolic pathways by which the liver miRNAs regulate pig feed efficiency.

• 농촌지도와개발
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• 제11권2호
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• pp.225-240
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• 2004

This study sought to search for the new alternatives and direction to develop and strengthening rural youth education in Korea. The study was conducted through literature review on articles, proceedings and other sources relevant to the pending issues and difficulties of rural youth education in Korea. Some of the critical needs for improving rural youth education in Korea included the following aspects or alternatives; 1) Strengthening the partnership between rural adult and youth would be necessary to gain broader support and to establish the increased awareness of adults for rural youth education. 2) Better coordination and cooperative efforts should be emphasized among various programs and institutions in implementing rural youth education policies and programs. 3) Developing a strong link between the agribusiness sector and the rural youth education system should be strengthened to produce the effective results. Further activities to develop and implement diverse programs and projects to cope with new trends In farming and agriculture as well as changing trends in marketing sector of agribusiness and rural communities in Korea. The rural leadership and community networks should be established for improving the effectiveness of rural youth educational activities.