• Title/Summary/Keyword: agricultural

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Re-identification of Colletotrichum gloeosporioides Species Complex Isolates in Korea and Their Host Plants

  • Le Dinh Thao;Hyorim Choi;Yunhee, Choi;Anbazhagan Mageswari;Daseul Lee;Dong-Hyun Kim;Hyeon-Dong Shin;Hyowon Choi;Ho-Jong Ju;Seung-Beom Hong
    • The Plant Pathology Journal
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    • v.40 no.1
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    • pp.16-29
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    • 2024
  • The Colletotrichum gloeosporioides species complex includes many phytopathogenic species, causing anthracnose disease on a wide range of host plants and appearing to be globally distributed. Seventy-one Colletotrichum isolates in the complex from different plants and geographic regions in Korea were preserved in the Korean Agricultural Culture Collection (KACC). Most of them had been identified based on hosts and morphological features, this could lead to inaccurate species names. Therefore, the KACC isolates were re-identified using DNA sequence analyses of six loci, comprising internal transcribed spacer, gapdh, chs-1, his3, act, and tub2 in this study. Based on the combined phylogenetic analysis, KACC strains were assigned to 12 known species and three new species candidates. The detected species are C. siamense (n = 20), C. fructicola (n = 19), C. gloeosporioides (n = 9), C. aenigma (n = 5), C. camelliae (n = 3), C. temperatum (n = 3), C. musae (n = 2), C. theobromicola (n = 2), C. viniferum (n = 2), C. alatae (n = 1), C. jiangxiense (n = 1), and C. yulongense (n = 1). Of these, C. jiangxiense, C. temperatum, C. theobromicola and C. yulongense are unrecorded species in Korea. Host plant comparisons showed that 27 fungus-host associations are newly reported in the country. However, plant-fungus interactions need to be investigated by pathogenicity tests.

A Review of Japanese Agricultural Mechanization Policy (일본 농업기계화 정책에 대한 고찰)

  • 이충호;강창용
    • Journal of Biosystems Engineering
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    • v.24 no.2
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    • pp.167-178
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    • 1999
  • In the path of agricultural development, agricultural mechanization has played some decisive roles such as protecting natural disaster, substitution of labor shortage in agricultural production and so on. In making our country's agricultural mechanization plan, Japanese agricultural mechanization policy has been frequently referred to but it's very hard to find informative data for systematic analysis of the mechanization policy. In this paper it was tried to analyze the resent trend in Japanese agricultural mechanization policy and it's performance with many materials published in Japan. As the results, four things are recommended to be considered in making Korean agricultural mechanization policy. First, faster enlargement of agricultural machinery size in Korea might be very harmful to drop down agricultural production cost. Second, diverse utilization methods of agricultural machniery should be introduced. Third, a marketing of the used agricultural machinery must be activated to reduce agricultural production cost. Lastly, the joint R & D for developing low price agricultural machniery is necessary for small markets.

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The Importance of Agricultural Ergonomics

  • Lee, Kwan-Suk
    • Journal of the Ergonomics Society of Korea
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    • v.30 no.4
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    • pp.437-441
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    • 2011
  • Objective: The objective this paper is to introduce the agricultural ergonomics and to show the importance of it in improving the agricultural works. Background: Despite of rapid advancement of technologies and adoption of modern mechanical equipments at farms in Korea, most of agricultural works have still remained manual and thus have regarded as one of the hardest works. With aging and feminization in rural areas, the need to improve agricultural works has grown quite much. Agricultural ergonomics have been known as a good tool in improving agricultural works around the world. Recently Korean government initiated a national program of providing assistive equipments to help farmers and a few ergonomists started to apply agricultural ergonomics in our rural villages as a part of this program. Method: This study reviewed how Ergonomics was used for the agricultural work. Results: Methods of Ergonomics which are used for the agricultural ergonomics and the simple procedure of the agricultural ergonomics were presented. Conclusion: It was found that agricultural works can be improved quite much using the agricultural ergonomics principle and the musculoskeletal stress of farmers can be reduced significantly.

Endomicrobial Community Profiles of Two Different Mealybugs: Paracoccus marginatus and Ferrisia virgata

  • Jose, Polpass Arul;Krishnamoorthy, Ramasamy;Gandhi, Pandiyan Indira;Senthilkumar, Murugaiyan;Janahiraman, Veeranan;Kumutha, Karunandham;Choudhury, Aritra Roy;Samaddar, Sandipan;Anandham, Rangasamy;Sa, Tongmin
    • Journal of Microbiology and Biotechnology
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    • v.30 no.7
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    • pp.1013-1017
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    • 2020
  • Mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) harbor diverse microbial symbionts that play essential roles in host physiology, ecology, and evolution. In this study we aimed to reveal microbial communities associated with two different mealybugs, papaya mealybug (Paracoccus marginatus) and two-tailed mealybug (Ferrisia virgata) collected from the same host plant. Comparative analysis of microbial communities associated with these mealybugs revealed differences that appear to stem from phylogenetic associations and different nutritional requirements. This first report on both bacterial and fungal communities associated with these mealybugs provides a preliminary insight on factors affecting the endomicrobial communities.

Characterization and cDNA Cloning of a Defensin-Like Peptide, Harmoniasin, from Harmonia axyridis

  • Kim, In-Woo;Lee, Joon-Ha;Park, Ha-Yan;Kwon, Young-Nam;Yun, Eun-Young;Nam, Sung-Hee;Kim, Seong-Ryul;Ahn, Mi-Young;Hwang, Jae Sam
    • Journal of Microbiology and Biotechnology
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    • v.22 no.11
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    • pp.1588-1590
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    • 2012
  • We compared the mRNA expression profile of the Harmonia axyridis larvae that were either untreated or treated with LPS. The extracted mRNAs were subjected to ACP RT-PCR analysis using a combination of arbitrary primers and oligo (dT) primer. Among the 47 DEGs differentially expressed, we identified a cDNA showing homology with defensin-like antibacterial peptide. The cDNA showed a putative 32-residue signal sequence and a 50-residue mature peptide named harmoniasin. We also investigated the antibacterial activity of the harmoniasin analog, which exhibited potent antibacterial activities against Gramnegative and -positive bacteria strains and it also evidenced no hemolytic activity.

Planning for Efficiency of Agricultural Extension in the Digital Age (디지털 시대 농촌지도 방법의 효율화 방안;지도매체로서의 인터넷 방송의 활용 구상을 중심으로)

  • Sung, Jong-Hwan;Chung, Han-Mo
    • Journal of Agricultural Extension & Community Development
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    • v.7 no.2
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    • pp.307-320
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    • 2000
  • Agricultural extension services in Korea need to build new extension system to cope with the digital age and especially with shortage of extension educators which has been originated from the changing status from central to local government. The new trends including development of multi-media, wide-spreading of internet, and advent of internet broadcasting are offering the opportunities for new agricultural extension service. Utilization of agricultural internet broadcasting could be a new alternative for enhancing the efficiency of agricultural extension services. Agricultural internet broadcasting could support decision making of farmers by forwarding unique agricultural data which have been collected, compiled and processed by the Rural Development Administration. Farmers will be able to search useful agricultural data through agricultural internet broadcasting for more efficient farm management and marketing farm products. Agricultural internet broadcasting could utilize more prompt and abundant agricultural technology and management data compiled by the Rural Development Administration, and through this process, agricultural internet broadcasting could compensate the shortage of extension manpower. Through feedback process of agricultural internet broadcasting. farmers will be able to communicate more efficiently with extension educators to increase agricultural production and income. Agricultural Internet broadcasting could be an alternative to prevent farmers from being shunned in the information-based society.

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Analysis of users of agricultural outlook information

  • Seungjee Hong;Ga Eul Kim;Seon Min Park;Sounghun Kim
    • Korean Journal of Agricultural Science
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    • v.49 no.4
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    • pp.833-843
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    • 2022
  • Since the supply and demand of agricultural products are unstable, which causes instability in farm income and consumer prices, the government has sought to mitigate the problems caused by unstable supply and demand by generating and providing agricultural outlook information. However, research should be carried out to increase the quality and utilization of agricultural observation information, because the value of agricultural observation information increases only when more users use this information and apply it to their decisions. In this study, a survey was conducted targeting producers and experts who are users or potential users of agricultural outlook information, and the results were analyzed through quantitative model, specifically importance-performance analysis (IPA) and analytic hierarchy process (AHP). The results show that improvement of promptness was required among the seven items evaluated with regard to agricultural outlook. Also, measures for dissemination of agricultural outlook information and the contents of outlook information should be improved to increase its use. If the quality level and use of agricultural observation information are increased by reflecting the results of the above analysis, decision-making on the supply and demand of agricultural products in Korea will be improved, and it is thought that it will be possible to increase farm household income and stabilize consumer prices through stabilization of supply and demand of agricultural products.

An Intelligent Spraying Machine Capable of Selective Spraying Corresponding to the Shape of Fruit Trees Using LiDAR (LiDAR를 활용한 과수 형상에 따라 선택적 방제가 가능한 지능형 방제기)

  • Yang, Changju;Kim, Gookhwan;Lee, Meonghun;Kim, Kyoung-Chul;Hong, Youngki;Kim, Hyunjong;Lee, Siyoung;Ryu, Hee-Suk;Kwon, Kyung-Do;Oh, Min-seok
    • Journal of Drive and Control
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    • v.17 no.4
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    • pp.23-30
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    • 2020
  • Driving on irregular and inclined roads using agricultural machinery such as spraying machines or trucks in orchards causes farmer casualties associated with the overturning of agricultural machinery. In addition, the harm to agricultural workers caused by the excessive inhalation of the scattered pesticide frequently occurs during pest control processes. To address these problems, we introduced precision agricultural technology that could selectively spray pesticides only where the fruit is present by recognizing the presence or shape of the fruit in the orchard. In this paper, a 16-channel LIDAR (VLP-16) made of Velodyne was used to identify the shape of fruit trees. Solenoid valves were attached to the end parts of 12 nozzles of the orchard spraying machine for on/off control. The smart spraying machine implemented in this way was mounted on a vehicle capable of autonomous travel and performed selective control depending upon the shape of the fruit trees while traveling in the orchards. This is expected to significantly reduce the amounts of pesticides used in orchards and production costs.

Discovery of markers for determining the maturity of silkworms by comparing gene expression patterns

  • Jong Woo Park;Chan Young Jeong;Hyeok Gyu Kwon;Seul Ki Park;Ji Hae Lee;Sang Kuk Kang;Seong-Wan Kim;Hyun-Bok Kim;Kee Young Kim;Chun Wan Park;Seong-Ryul Kim
    • International Journal of Industrial Entomology and Biomaterials
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    • v.47 no.1
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    • pp.51-62
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    • 2023
  • The advantages of silkworms as functional foods are well known and various products are being developed. In general, silkworms sold in the market include silkworm powder (3 days of fifth instars) and SukJam (7 days or more of fifth instars), In other words, product classification is made according to the maturity of the fifth instar silkworms. In this study, we analyzed the gene expression changes in the fifth instar silkworms and attempted to validate the use of deregulated genes in maturity analysis. After rearing BaekokJam, transcriptome analysis was performed on days 1, 3, 5, and 8 days of the fifth instar, and differentially expressed genes showing differences at each period were selected. Of the 31,841 contigs analyzed, 4012 contigs were identified with a log2 fold change of two or more between 5 and 8 days of the fifth instar. RT-PCR was performed for 18 contigs, which showed increased or decreased expression, but in c127159, c97909, c96974, c119920, c42251, and c80216 showed clear differences. To identify SukJam, a combination of the contigs c127159 (180 bp), c97909 (143 bp), and c80216 (120 bp) was amplified. Taken together, these results suggest that the harvest time of silkworms can be determined using gene expression pattern analysis.