• Title/Summary/Keyword: agitation system

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Reproducibility of Reaeration in Sewer using Batch Reactor Test (실험반응조를 이용한 하수관에서의 재포기현상 재현 가능성에 관한 연구)

  • Hwang, Hwankook;Min, Sangyun;Cho, Jinkyu
    • Journal of the Korean GEO-environmental Society
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    • v.15 no.8
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    • pp.45-50
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    • 2014
  • The microorganism decomposition experiment of sewage in the underground sewer has the limit of experiment condition and time. The way to reproduce the microorganism decomposition in the underground sewer was studied using batch reactor setting up the DO as a limiting condition. The DO concentration in the sewer is controlled by reaeration. It is possible to obtain correlation between flow condition and reaeration coefficient through the reproduction of reaeration phenomenon by controlling the flow condition in the sewer using this phenomenon. And it is possible to set the flow condition and agitation intensity (velocity gradient) that has the same reaeration coefficient using the correlation between the reaeration coefficient with the flow condition and reaeration coefficient with the agitation intensity. The circumstances in the sewer system was reproduced using batch reactor setting up the DO as a limiting condition from these results.

Effects of Mixing Performance and Conditioned Medium on hCTLA4Ig Production in Transgenic Rice Cell Suspension Cultures (형질전환 벼 현탁세포 배양에서 혼합효율과 조정배지가 hCTLA4Ig 생산에 미치는 영향)

  • Choi, Hong-Yeol;Park, Jun-Yong;Nam, Hyung-Jin;Gong, Mi-Kyung;Yoo, Ye-Ri;Kim, Dong-Il
    • KSBB Journal
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    • v.30 no.6
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    • pp.307-312
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    • 2015
  • Transgenic rice cells using RAmy3D promoter can provide high productivity, and the production of recombinant protein is induced by sugar starvation. In this system, productivity was reduced during the scale-up processes. To ensure the influences of shear stress and oxygen transfer rate, working volume and mixing performances were investigated under various agitation speeds and working volumes. In addition, inoculation methods including suspended cells and filtered cells were compared. Working volumes and shaking speeds were 300, 450 mL and 80, 120 rpm, respectively. Hydrodynamic environment of each condition was measured numerically like mixing time and $k_La$. Good mixing performance and high shear stress were measured at high agitation speed and low volume. The highest level of hCTLA4Ig was 30.7 mg/L at 120 rpm, 300 mL. When conditioned medium was used for inoculation, increased cell growth was noticed during the day 0~4 and decreased slower than filtered cells. Compared with filtered cells, the maximum hCTLA4Ig level reached 37.8 mg/L at 120 rpm, 300 mL and lower protease activity level was observed. In conclusion mixing performance is critical factor for productivity and conditioned medium can have a positive effect on damaged cells caused by hydrodynamic shear stress.

Experimental study on the influence of heating surface inclination angle on heat transfer and CHF performance for pool boiling

  • Wang, Chenglong;Li, Panxiao;Zhang, Dalin;Tian, Wenxi;Qiu, Suizheng;Su, G.H.;Deng, Jian
    • Nuclear Engineering and Technology
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    • v.54 no.1
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    • pp.61-71
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    • 2022
  • Pool boiling heat transfer is widely applied in nuclear engineering fields. The influence of heating surface orientation on the pool boiling heat transfer has received extensive attention. In this study, the heating surface with different roughness was adopted to conduct pool boiling experiments at different inclination angles. Based on the boiling curves and bubble images, the effects of inclination angle on the pool boiling heat transfer and critical heat flux were analyzed. When the inclination angle was bigger than 90°, the bubble size increased with the increase of inclination angle. Both the bubble departure frequency and critical heat flux decreased as the inclination angle increased. The existing theoretical models about pool boiling heat transfer and critical heat flux were compared. From the perspective of bubble agitation model and Hot/Dry spot model, the experimental phenomena could be explained reasonably. The enlargement of bubble not only could enhance the agitation of nearby liquid but also would cause the bubble to stay longer on the heating surface. Consequently, the effect of inclination angle on the pool boiling heat transfer was not conspicuous. With the increase of inclination angle, the rewetting of heating surface became much more difficult. It has negative effect on the critical heat flux. This work provides experimental data basis for heat transfer and CHF performance of pool boiling.

Composting Methods for Pig Sludge and the Stabilized Investigation of Crop Cultivation (돈분의 자원화 퇴비 제조 방법 및 작물 재배 안전성 검정)

  • Oh, Tae-Seok;Kim, Chang-Ho
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.29 no.1
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    • pp.51-62
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    • 2009
  • This study was carried out development a new composting system to lower copper and zinc concentration in plg sludge compost and conduct an inquiry into the possibility of crop cultivation. The concentrations of nitrogen, phosphorus and pH averaged 4.4%, 6.3% and 7.57, respectively, which were higher concentrations than in commercial organic fertilizers, and the concentrations of copper and zinc averaged 805 and 1,704 mg/kg, respectively, which were beyond the heavy metal concentration limit in byproduct compost. Hydrated citric acid I lowered the concentrations of copper and zinc by 58% and 97%, respectively and hydrated oxalic acid II lowered the concentrations of copper and zinc by 48% and 56%, respectively in pig sludge compost. Lower concentrations of copper and zinc in pig sludge resulted from the enhanced hydrated-citric acid concentration in organic acid solution mixed with distilled water. The concentrations of copper and zinc were 330, and 41 mg/kg in the pig sludge treated with 100% hydrated citric acid. Agitation composting system stabilized the compost earlier than the stationary composting system, in which the stabilization condition was confirmed by higher temperature by $4^{\circ}C$ at highest temperature and 7 days earlier cooling down after highest temperature. The levels of germination index (G.I) 80 were obtained 15 and 20 days after composting in agitation and stationary composting system, respectively. The concentrations of copper and zinc were 2.4 and 4.26 mg/kg respectively in soils amended with pig sludge compost after removing process of heavy metals by citric acid, but 8.0 and 22.37 mg/kg, respectively in soils amended with Pig Sludge. The concentrations of heavy metals was highest in com cultivated in soils amended with pig sludge. The copper and zinc concentrations In corn leaves were 75.2 and 50.56 mg/kg respectively, which were 4 and 2 fold higher than the com cultivated in soils amended with pig sludge compost after heavy metal removing process by hydrated citric acid.

Cloud and Fog Computing Amalgamation for Data Agitation and Guard Intensification in Health Care Applications

  • L. Arulmozhiselvan;E. Uma
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.18 no.3
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    • pp.685-703
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    • 2024
  • Cloud computing provides each consumer with a large-scale computing tool. Different Cyber Attacks can potentially target cloud computing systems, as most cloud computing systems offer services to many people who are not known to be trustworthy. Therefore, to protect that Virtual Machine from threats, a cloud computing system must incorporate some security monitoring framework. There is a tradeoff between the security level of the security system and the performance of the system in this scenario. If strong security is needed, then the service of stronger security using more rules or patterns is provided, since it needs much more computing resources. A new way of security system is introduced in this work in cloud environments to the VM on account of resources allocated to customers are ease. The main spike of Fog computing is part of the cloud server's work in the ongoing study tells the step-by-step cloud server to change the tremendous measurement of information because the endeavor apps are relocated to the cloud to keep the framework cost. The cloud server is devouring and changing a huge measure of information step by step to reduce complications. The Medical Data Health-Care (MDHC) records are stored in Cloud datacenters and Fog layer based on the guard intensity and the key is provoked for ingress the file. The monitoring center sustains the Activity Log, Risk Table, and Health Records. Cloud computing and Fog computing were combined in this paper to review data movement and safe information about MDHC.

Decontamination of Bacterial Spores Using a Bio-reaction System (생물 반응 시스템을 활용한 세균 포자 제독 시험)

  • Yeonghwan Seo;Hanwool Park;Hongsuk Lee;Seongjoo Kim
    • Journal of the Korea Institute of Military Science and Technology
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    • v.27 no.5
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    • pp.649-656
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    • 2024
  • This report describes the test results of decontamination of Bacillus atrophaeus spores. The spore solution at 109 cfu/mL concentration was treated with chemical decontaminants, hydrogen peroxide, DF-200, sodium dichloroisocyanurate(NaDCC), and perasasfe. DF-200 was not suitable for decontamination of the spore solution. Among them, the optimal decontamination reaction conditions of perasafe and NaDCC were established from test tube experiments in a mL-scale, and they were validated in a bio-reaction system in a L-scale. The optimal conditions of perasafe and NaDCC were 1.0 % of concentration with 5 minutes of reaction time and 0.3 % of concentration with 10 minutes of reaction time, respectively. Also, both decontaminants required adequate agitation for decontamination. The results in the bio-reaction system were similar with the results from the smaller scale using test tubes, showing that the optimal conditions from the smaller scale experiments can be applied to the larger scale.

Performance of a Novel Bioreactor Equipped with Moving Membrane Tube-Aeration System (회전하는 산소전달장치가 부착된 동물세포 배양기의 조업 성능에 관한 고찰)

  • Kim, Young-Nam;Jeon, Byung-Cheol;Lee, Jin-Ha;Lee, Hyeon-Yong
    • Microbiology and Biotechnology Letters
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    • v.21 no.4
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    • pp.348-353
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    • 1993
  • The optimal conditions for operating a moving-aeration bioreactor were determined as 30rpm and 150 (ml/min) of air flow rate, which can yield ca. 7.3 (l/h)of maximum mass transfer coefficient. It was also found that the agitation speed played much much important role than air input rate in oxgen transfer into the medium. $2.6{\times}10^6$ (cells/ml) and 0.6 (ml/l) of maximum cell denisty and IL-2 production were observed in batch cultivation of IL-2 producing BHK cell line. 0.53 (mM/l/h) of oxygen uptake rate was also estimated. The performance of a moving-aeration bioreactor (specific growth rate and oxygen uptake rate, etc.) was superior to other culture systems, such as cell-life and static membrane aeration bioreactors. Ii must be useful to apply this reactor to many culture processes by improving structural limitations in scaling-up the system.

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Implementation of a silicon sludge recycling system for solar cell using multiple centrifuge (다중 원심분리법을 이용한 태양전지용 실리콘 폐 슬러지 재생 시스템 구현)

  • Kim, Ho-Woon;Choi, Byung-Jin
    • Journal of Korea Society of Industrial Information Systems
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    • v.17 no.1
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    • pp.1-9
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    • 2012
  • This paper explained about the sludge recycling system which retrieved the silicon and abrasive from solar cell wafer slicing. The basic process of the recycling system was multiple centrifuge and secondary processes of ultra sonic agitation, addition of alcohol-water solution and heating sludge was added for raising separation efficiency. The recycling rate was about 96% and 94% for 2N, 4N silicon respectively. The SiC abrasive recycling rate was about 80%. To retrieve the high purity of 4N silicon, the heat process in vacuum furnace was added to remove remaining impurity components.

Preparation of Ultra Fine Poly(methyl methacrylate) Microspheres in Methanol-enriched Aqueous Medium

  • Shim, Sang-Eun;Kim, Kijung;Sejin Oh;Soonja Choe
    • Macromolecular Research
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    • v.12 no.2
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    • pp.240-245
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    • 2004
  • Monodisperse PMMA micro spheres are prepared by means of a simple soap-free emulsion polymerization in methanol-enriched aqueous medium in a single step process. The size and uniformity of the microspheres are dependent on the polymerization temperature. In a stable system, the uniformity is improved with the polymerization time. The most uniform and stable micro spheres are obtained under mild agitation speed of 100 rpm at 70$^{\circ}C$. The monodisperse PMMA microspheres in the size range of 1.4-2.0 $\mu\textrm{m}$ having less than 5% size variation are successfully achieved with varying concentrations of monomer and initiator. As the monomer and initiator concentrations increase, the larger micro spheres with enhanced uniformity are obtained. However, the decreased amount of water induces the polydisperse PMMA particles due to the generation of secondary particles.

Studies on the Fermentation of Fish Protein -1. A Model Design of Fermentor- (수산 발효식품 제조에 관한 연구 -1. 어육 발효조의 설계-)

  • Lee, Kang-Ho;Choi, Ho-Yeon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.1 no.1
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    • pp.51-62
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    • 1972
  • In Korea, fermented fish has been playing an important role as a preserved and flavor rich food. It is said that the digestion of fish protein is due to both action of intrinsic (autolytic enzymes) and bacterial enzymes in fish. The mass production of fermented fish has been impeded since traditional method of fermentation requires a long duration for a complete digestion. A high concentration of salt and unsanitary condition are also considered disadvantages of the old method. To improve the quality of the product and to develop mechanized process of fermentation, fermentors which have such control device as temperature, pH and agitation control system have been urgently needed. In this study, a model design of a fermentor is studied. The calculation was based on the optimum conditions for enzymatic hydrolysis of fish protein which involve temperature, pH, viscosity and other factors.

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