• Title/Summary/Keyword: agent-based control

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Isolation and Identification of Stenotrophomonas maltophilia BW-13 Active Against Rhizoctonia solani Causing Crisphead Lettuce Bottom Rot (Rhizoctonia solani에 의한 결구상추 밑둥썩음병 방제균주 Stenotrophomonas maltophilia BW-13의 분리 및 동정)

  • Kim Han-Woo;Park Jong-Young;Kim Hyun-Ju;Lee Kwang-Youll;Lee Jin-Woo;Choi Woobong;Lee Seon-Woo;Moon Byung-Ju
    • Research in Plant Disease
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    • v.11 no.2
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    • pp.152-157
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    • 2005
  • In a course of searching for biofungicide to control crisphead lettuce bottom rot caused by Rhizoctonia solani, we have isolated an antagonistic bacterium from lettuce rhisophere soil. A total of 702 bacterial isolates were isolated and tested for in vitro growth inhibition of R. solani. Seven strains appeared to have strong antagonistic effect against R. solani in in vitro growth inhibition assay. In the pot experiments, a strain BW-13 showed the most potent disease control effect on the both lettuce seedlings and adults plants. Therefore, the BW-13 was selected as a biocotrol candidate against crisphead lettuce bottom rot. Based on its morphology, physiological characteristics, and 165 rRNA gene analysis, the BW-13 was finally identified as Stenotrophomonas maltophilia. This study indicated that S. maltophilia BW-13 could be used as a biocontrol agent to control crisphead lettuce bottom rot.

Remediation of Pb-Contaminated Soil by Soil Washing using Hdrochloric Acid (염산을 사용한 납 오염 토양의 토양 세척에 의한 정화)

  • Baek, Ki-Tae;Kim, Do-Hyung;Seo, Chang-Il;Yang, Jung-Seok;Lee, Jae-Young
    • Journal of Soil and Groundwater Environment
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    • v.12 no.3
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    • pp.17-22
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    • 2007
  • The feasibility of soil washing was investigated to remediate Pb-contaminated field soil. Hydrochloric acid was used as a washing agent. As mixing time increased from 5 min to 120 min, removal efficiency of Pb from contaminated soil increased from 69.3% to 81.9%. Two times washing with 0.2 M HCl showed 96% removal efficiency even at mixing time of 10 min. The Pb content in soil increased sharply as particle size of soil decreased, and removal efficiency was highly dependent on mixing time and temperature. Based on this result, acid washing technologies can be applied to remediate the Pb-contaminated soil used in this study.

Development of SIP based Call Processing Language Server System (SIP기반 호 처리 언어(CPL) 서버 시스템의 설계 및 구현)

  • Yi Jong-Hwa;Min Kyung-Joo;Kang Shin-Gak
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.1B
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    • pp.101-108
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    • 2004
  • SIP(Session Initiation Protocol) is a suitable protocol for supporting Internet telephony services and currently market requirements fur developing supplementary telephony services such as unconditional call forwarding, call forwarding on busy or no answer, call filtering services have recently grown. CPL(Call Processing Language) is a standard technology that can be used to describe and control internet telephony services. In this paper, we describe the CPL system for supplementary Internet telephony services using SIP as an application level call signaling protocol. Those supplementary services are composed of CPL client which is a SIP UA, SIP Proxy server, Registrar and CPL server In this paper, we describe the design and implementation of the CPL server system in detail which is developed in Linux 7.2 using C and C++ programming languages.

Immunomodulatory Effects of Hexane Insoluble Fraction of Ficus septica Burm. F. in Doxorubicin-treated Rats

  • Nugroho, Agung Endro;Hermawan, Adam;Nastiti, Kunti;Suven, Suven;Elisa, Pritha;Hadibarata, Tony;Meiyanto, Edy
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.11
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    • pp.5785-5790
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    • 2012
  • The use of chemotherapeutics induces cardiotoxicity and affects immune functions, therefore development of combinatorial agents against cardiotoxicity and immunosuppression needs to be explored. Previous studies of the hexane insoluble fraction (HIF) of an ethanolic extract of Ficus septica leaves showed anticancer effects singly and in combination with doxorubicin on T47D breast cancer cells. In this present study, it was evaluated for its immunomodulatory activities in doxorubicin-treated rats. Thirty male Sprague Dawley rats were divided into five groups consisting of six rats each as follows: Group 1, receiving oral saline 10 ml/kg BW (control group); Group 2, receiving HIF dose 750 mg/kg BW orally, once daily; Group 3, receiving HIF dose 1.500 mg/kg BW orally, once daily; Group 4, given oral saline 10 ml/kg BW (normal group); Group 5, receiving HIF dose 1.500 mg/kg BW orally, once daily. The rats of group 1-3 were intramuscularly administered with doxorubicin at a dose of 4.67 mg/kg BW at the days 1 and 4 to suppress immune functions. Concomitantly, the rats were treated with saline or HIF for seven consecutive days (1 to 7). Treatment of HIF succeeded in reducing side effects of doxorubicin based on increasing lymphocyte density and phagocytosis activity and capacity of macrophages, as well as increasing the CD8+ blood level and decreasing spleen IL-10 expression. Hexane insoluble fraction of of ethanolic extract of Ficus septica leaves has potential as a protective agent combined with doxorubicin.

The Effect of Agricultural Wastes on Rice Plant Growth (답토양(畓土壤)의 유기물(有機物) 시용효과(施用效果))

  • Lee, Sang-Kyu;Park, Jun-Kyu
    • Applied Biological Chemistry
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    • v.27
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    • pp.56-67
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    • 1984
  • As in many other country, the use of organic matter in Korea has long history. Farmers understand the value of organic matter as the source of plant nutrient and soil improving agent in general. Since 50 years ago, the sources of organic matter in paddy soils were compost, rice and barly straw, green manure, animal waste, fish and beancake, etc.. Application of green manures such as vetch and chinese milk vetch showed no significant effect on the yield of brown rice in paddy soil. On the other hand, the effects of compost and rice straw showed more significant on the yield of brown rice in paddy soil. Application of rice straw in rice cultivation is commonly made at different times between harvest, early spring and several weeks before transplanting. Considering the suitable paddy soil for application of rice straw under well to moderately well drained soil, the yield was pronounced more than poorly drained soil. Based on laboratory and field experimants, application of rice straw promoted the decrease of oxidation-reduction potential in well to moderately well drained soil. This results to be enhanced the release of some mineral nutrients,. such as potassium, calcium, silicon, and increase of availability of soil phosphorus. In the field experiments, results obtained from nitrogen fraction on the immobilization-mineralization of the tracer nitrogen applied in paddy soil,the amount and index of organic nitrogen incoporated in soil was more pronounced in rice straw application than control. Rice straw and its transformation products incoporated in the soil, provided the inflow of energy necessary to maintain heterotrophic microbes activities. Rice straw and its transformation products, especially soluble carbohydrate, enhanced the population of free-living heterotrophic $N_2$ - fixing microbes. Moreover, rice straw and its transformation products in paddy soil, enhanced the activities of soil enzymes such as dehydrogenase and urease.

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Dyeing Behavior of Silk/CDP Mixed Filament with a Cationic Dye (캐티온염료를 이용한 Silk/Cationic Dyeable Polyester 혼합사의 염색거동)

  • Choe, Jong-Mun;Gwon, Hae-Yong;Park, Yeong-Hwan
    • Journal of Sericultural and Entomological Science
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    • v.37 no.2
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    • pp.154-160
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    • 1995
  • Dyeing behavior of Silk/Cationic dyeable polyester(CDP) mixed filament was investigated by using a cationic dye. The effect of pH, temperature and additives such as carrier, levelling agent and salt were examined for each silk and CDP component of mixed filament in order to find out the optimum dyeing condition. Based on these results, the dyeing behavior was investigated for Silk/CDP mixed filaments concering various dyeing parameters. The dye adsorption was significantly changed on the dyeing temperature, carrier addition and pH of the dyebath. Specially, the dye migration phynomena were observed for a mixed filament, showing that the dyes initially observed on the surface of silk fiber migrate to the CDP component during a dyeing process. The dyeing of Silk/CDP mixed filaments accompanied by dye migration and as a result, dye adsorption can be developed only for a CDP component. Therefore, the control of dyeing temperature is most important for a Silk/CDP mixed filament, in order that both silk and CDP component are able to be dyed by a cationic dye simultaneously in one bath/one dye system.

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Molecular Discrimination of Cervidae Antlers and Rangifer Antlers

  • Kim, Eun-Jin;Jung, Young-Ja;Kang, Shin-Jung;Chang, Seung-Yup;Huh, Keun;Nam, Doo-Hyun
    • BMB Reports
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    • v.34 no.2
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    • pp.114-117
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    • 2001
  • Cervi Parvum Cornu is widely used as a hemopoietic, tonifying, growth-promoting, cardiotonic, and immuno-modulating agent in Korea. In order to develop the quality control method of Cervi Parvum Cornu by the identification of the biological source or origin, the molecular approach was applied using PCR (polymerase chain reaction) and PCR-RFLF (PCR-restriction fragment length polymorphism) analysis. In the PCR analysis of the mitochondrial 12S rRNA gene and cytochrome b gene regions, no distinctive DNA bands from Cervidae (deer) antlers and Rangifer (reindeer) antlers were observed. However, when the amplified products in the mitochondrial cytochrome b gene region were subjected to restriction digestion with TaqI, Cervidae antlers showed an undigested state of 380 by band, differently from two bands of 230 by and 1S0 by from Rangifer antlers. Based on this finding, the base sequences of amplified PCR products in the range of mitochondria) cytochrome b gene from Cervidae antlers and Rangifer antlers were determined and subjected to restriction analysis by various endonucleases. The results showed that antlers from Rangifer species could be simply discriminated with other antlers from 8 Cervidae species (Chinese deer, Russian deer, Hong Kong deer, New Zealand deer, Kazakhstan deer, elk, red deer and Sika deer) by PCR-RFLP analysis using AtuI, HaeIII, HpaII or Sau3AI(MboI) as well as TaqI in the range of the mitochondrial cytochrome b gene.

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Filtering and Intrusion Detection Approach for Secured Reconfigurable Mobile Systems

  • Idriss, Rim;Loukil, Adlen;Khalgui, Mohamed;Li, Zhiwu;Al-Ahmari, Abdulrahman
    • Journal of Electrical Engineering and Technology
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    • v.12 no.5
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    • pp.2051-2066
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    • 2017
  • This paper deals with reconfigurable secured mobile systems where the reconfigurability has the potential of providing a required adaptability to change the system requirements. The reconfiguration scenario is presented as a run-time automatic operation which allows security mechanisms and the addition-removal-update of software tasks. In particular, there is a definite requirement for filtering and intrusion detection mechanisms that will use fewer resources and also that will improve the security on the secured mobile devices. Filtering methods are used to control incoming traffic and messages, whereas, detection methods are used to detect malware events. Nevertheless, when different reconfiguration scenarios are applied at run-time, new security threats will be emerged against those systems which need to support multiple security objectives: Confidentiality, integrity and availability. We propose in this paper a new approach that efficiently detects threats after reconfigurable scenarios and which is based on filtering and intrusion detection methods. The paper's contribution is applied to Android where the evaluation results demonstrate the effectiveness of the proposed middleware in order to detect the malicious events on reconfigurable secured mobile systems and the feasibility of running and executing such a system with the proposed solutions.

Whole-Blood Gene-Expression Profiles of Cows Infected with Mycobacterium avium subsp. paratuberculosis Reveal Changes in Immune Response and Lipid Metabolism

  • Shin, Min-Kyoung;Park, Hong-Tae;Shin, Seung Won;Jung, Myunghwan;Im, Young Bin;Park, Hyun-Eui;Cho, Yong-Il;Yoo, Han Sang
    • Journal of Microbiology and Biotechnology
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    • v.25 no.2
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    • pp.255-267
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    • 2015
  • Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne's disease, a chronic debilitating disease affecting ruminants worldwide. In the present study, we aimed to determine the major gene networks and pathways underlying the immune response to MAP infection using whole-blood cells, as well as provide the potential transcriptional markers for identifying the status of MAP infection. We analyzed the transcriptional profiles of whole-blood cells of cattle identified and grouped according to the presence of MAP-specific antibodies and the MAP shed by them. The grouping was based on the results obtained by ELISA and PCR analyses as follows: i) Test1 group: MAP-negative results obtained by ELISA and positive results obtained by PCR; ii) Test2 group: MAP-positive results obtained by ELISA and negative results obtained by PCR; iii) Test3 group: MAP-positive results obtained by ELISA and positive results obtained by PCR; iv) uninfected control: MAP-negative results obtained both by ELISA and PCR analysis. The results showed down-regulated production and metabolism of reactive oxygen species in the Test1 group, activation of pathways related to the host-defense response against MAP (LXR/RXR activation and complement system) in the Test2 and Test3 groups, and anti-inflammatory response (activation of IL-10 signaling pathway) only in the Test3 group. Our data indicate a balanced response that serves the immune-limiting mechanism while the host-defense responses are progressing.

Effects of Soy Protein and Isoflavones on Bone Markers and Hormones in Growing Male Rats (콩 단백질과 Isoflavones가 성장기 수컷 흰쥐에서 골 지표와 호르몬에 미치는 영향)

  • 최미자
    • Journal of Nutrition and Health
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    • v.36 no.5
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    • pp.452-458
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    • 2003
  • Soybean is a rich source of isoflavones such as genistein and daidzein. Soy isoflavones have both weak estrogenic and anti-estrogenic effects and are structurally similar to tamoxifen, an agent that has an effect similar to that of estrogen in terms of reducing postmenopausal bone loss. The purpose of this study was to determine the effects of differences in protein source (casein vs soy) and isoflavone levels (reduced vs higher levels) on selected bone markers and hormones in growing male rats. Thirty weanling Sprague-Dawley young rats were divided into 3 groups: The control group was fed a casein-based diet, the soy concentrate group was fed soy protein with totally reduced isoflavones content (isoflavones 0.07 mg/g protein), and the soy isolate group was fed soy protein with a higher than normal isoflavones content (isoflavones 3.4 mg/g protein). The degree of bone formation was estimated by measuring serum osteocalcin and alkaline phosphoatase (ALP). By determining collagen cross-linkage by immunoassay and correcting with creatinine values, the bone resorption rate was compared. Serum osteocalcin, growth hormone, estrogen and calcitonin were analyzed using radio immunoassay kits. The bone formation marker and ALP activity were differentiated by protein source, showing higher values than casein in feeding either soy isolate or soy concentrate. In this study using growing rats, the differences in isoflavone contents were not a significant factor in either bone formation or bone reaborption markers. Moreover, the soy isolate group had significantly higher levels of growth hormone than the casein group. The findings of this study suggest that growth hormone is partially responsible for its bone-formation effects in young growing rats. Soy protein and the isoflavones in soy protein are beneficial for bone-formation in growing male rats. Therefore, exposure to soy protein and isoflavones early in life may have long-term health benefits in preventing bone diseases such as osteoporosis. Further study to evaluate the mechanism of action of isoflavones on bones is warranted. (Korean J Nutrition 36(5): 452∼458, 2003)