• Nuclear Engineering and Technology
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• 제54권8호
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• pp.3017-3026
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• 2022

The ¹³¹Cs radioisotope with a short half-life time and high average radiation energy can treat the cancer effectively in prostate brachytherapy. The typical ¹³¹Cs production processes have a separation step of the cesium from ¹³¹Ba to obtain a high specific radioactivity. Herein, we suggested a novel ¹³¹Cs separation method based on the Ba²⁺ adsorption of alginate beads. It is necessary to reduce the affinity of alginate beads to cesium ions for a high production yield. The carboxyl group of the alginate beads was replaced by a sulfonate group to reduce the cesium affinity while reinforcing their affinity to barium ions. The modified beads exhibited superior Ba²⁺ adsorption performances to native beads. In the fixed-bed column tests, the saturation time and adsorption capacity could be estimated with the Yoon-Nelson model in various injection flow rates and initial concentrations. In terms of the Cs elution, the modified alginate showed better performance (i.e., an elution over 88%) than the native alginate (i.e., an elution below 10%), indicating that the functional group modification was effective in reducing the affinity to cesium ions. Therefore, the separation of cesium from the barium using the modified alginate is expected to be an additional option to produce ¹³¹Cs.

• 한국초전도ㆍ저온공학회논문지
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• 제25권4호
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• pp.60-64
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• 2023

The manufacturing process of antibody drugs comprises two main stages: the upstream process for antibody cultivation and the downstream process for antibody extraction. The domestic bio industry has excellent technology for the upstream process. However, it relies on the technology of foreign countries to execute downstream process such as affinity chromatography. Furthermore, there are no domestic companies capable of producing the equipment for affinity chromatography. High gradient magnetic separation technology using a high temperature superconducting magnet as a novel antibody separation and purification technology is introduced to substitute for the traditional technology of affinity chromatography. A specially designed magnetic filter was equipped in the bore of the superconducting magnet enabling the continuous magnetic separation of nano-sized paramagnetic beads that can be used as affinity magnetic nano beads for antibodies. To optimize the magnetic filter that captures superparamagnetic nanoparticles effectively, various shapes and materials were examined for the magnetic filter. The result of magnetic separation experiments show that the maximum separation and recovery ratio of superparamagnetic nanoparticles are 99.2 %, and 99.07 %, respectively under magnetic field (3 T) and flow rate (600 litter/hr).

• BMB Reports
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• 제45권4호
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• pp.233-238
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• 2012

We present a top down separation platform for yeast ribosomal proteins using affinity chromatography and capillary electrophoresis which is designed to allow deposition of proteins onto a substrate. FLAG tagged ribosomes were affinity purified, and rRNA acid precipitation was performed on the ribosomes followed by capillary electrophoresis to separate the ribosomal proteins. Over 26 peaks were detected with excellent reproducibility (<0.5% RSD migration time). This is the first reported separation of eukaryotic ribosomal proteins using capillary electrophoresis. The two stages in this workflow, affinity chromatography and capillary electrophoresis, share the advantages that they are fast, flexible and have small sample requirements in comparison to more commonly used techniques. This method is a remarkably quick route from cell to separation that has the potential to be coupled to high throughput readout platforms for studies of the ribosomal proteome.

• 멤브레인
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• 제1권1호
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• pp.34-43
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• 1991

물에 대해 수착선택성이 높은 아세트산 셀룰로오스 치밀막(CA막)과 에탄올에 대해 수착선택성이 높은 실리콘 고무막(SR막)을 통한 물(1)/에탄올(2) 이성분 혼합액의 투과증발에 있어서 막의 친화성과 최적 조업조건의 관계를 조사하였다. CA막과 SR막을 제조하여, 이들 막에서의 수착량, 수착선택도, 투과증발 분리도 및 투과증발 속도를 실험으로 구하고 서로 비교하였다. 하류압력의 영향을 Thompson다이아그램을 써서 분석하였고, 공급액 농도와 조업 온도 변화에 따른 수착 및 투과증발 특성을 활동도 계수, 가소화 효과, 활성화에너지 등으로 설명할 수 있었다. CA막을 사용한 물의 분리의 경우에는 물에 대한 투과증발 분리도 ($[\alpha^2_1]_{PV}$) 및 투과증발 속도 모두 하류압력이 낮고, 공급액의 농도가 중간 정도이고, 온도가 높은 조업조건이 유리하였다. 그러나 SR막을 사용한 에탄올의 분리의 경우에는 에탄올에 대한 투과증발 분리도 ($[\alpha^2_1]_{PV}$) 는 하류압력이 높을수록, 공급액 중의 에탄올 농도가 낮을수록, 조업온도가 낮을수록 증가하였지만, 투과증발 속도는 반대의 경향을 나타내었다.

• 멤브레인
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• 제19권2호
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• pp.113-121
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• 2009

실리카 입자를 기공 형성제로 사용하여 물리적 강도와 단백질 결합용량이 높은 다공성 키토산 및 키틴 친화 막을 제조하였다. 키토산 친화 막의 BSA 단백질 결합용량은 최대 21.8mg/mL이었으며, 키틴 친화 막의 lysozyme 효소 결합용량은 최대 26.1mg/mL이었다. 제조된 다공성 키토산 및 키틴 친화 막을 사용하여 단백질 용액의 loading 유량, loading 양 및 농도 변화에 따른 BSA와 lysozyme의 친화 막 여과 크로마토그래피 분리 실험을 수행하였다. 친화 막 여과 크로마토그래피 분리 실험을 통해 얻어진 loading/washing/elution의 단계로 구성된 일련의 크로마토그램으로부터 단백질 용출량과 결합수율을 구하였다. 키토산 및 키틴 친화 막에의 BSA 및 lysozyme 단백질의 결합량과 결합수율은 loading용액의 유량이 작을수록, 주입량 및 농도가 클수록 증가하였다. 이 결과로부터 실리카 입자를 기공 형성제로 사용하여 제조된 다공성 키토산 및 키틴 막은 단백질의 대규모 여과 크로마토그래피 분리를 위한 친화 막으로서 효과적인 활용이 기대된다.

• 한국막학회:학술대회논문집
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• 한국막학회 2004년도 Proceedings of the second conference of aseanian membrane society
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• pp.59-62
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• 2004

Affinity membranes have emerged principally to overcome the problems of limited specificity experienced with membranes that operate purely on a sieving mechanism and as an alternative to the traditional affinity resins. It is a logical expectation that affinity membranes might combine the outstanding selectivity of affinity resins with the high productivity associated with filtration membranes.(omitted)

• 환경위생공학
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• 제12권2호
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• pp.59-64
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• 1997

For purification of a 70kDa toxic protein of mosquitocidal delta-endotoxin from B. thuringiensis strain H9B, immuno-affinity chromatography was performed. After separation of 70kDa toxic proteins from the delta-endotoxin of the strain H9B on SDS-PAGE, the 70kDa toxic protein was subcutaneously injected into rabbit for making a polyclonal antibody. A anti-70kDa toxic protein was purified by a column chromatography packed with protein A-sepharose 4B gels. The 70kDa toxic protein from delta-endotoxin of the strain H9B was also purified by an immuno-affinity chromatography packed with CNBr-activated sepharose 4B gels conjugated anti-70kDa toxic protein after elution with 1/10M citric acid-1/5M Na$_{2}$HPO$_{4}$ buffer(pH3.2) containing 0.5M NaCl. The 70kDa toxic protein was purified through only one step-separation system, was demonstrated by SDS-PAGE and immunoblot.

• Biotechnology and Bioprocess Engineering:BBE
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• 제8권4호
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• pp.240-245
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• 2003

The use of microfabricated microfluidic devices offers significant advantages over current technologies including fast analysis time and small reagent requirements. In the context of proteomic research, the possibility of using affinity-based separations for prefractionation of samples using microfluidic devices has significant potential. We demonstrate the use of microscale devices to achieve affinity separations of proteins using a device fabricated from borosilicate glass wafers. Photolithography and wet etching are used to pattern individual glass wafers and the wafers are fusion bonded at 650$^{\circ}C$ to obtain enclosed channels. A polymer has been successfully polymerized in situ and used either as a frit for packing beads or, when derivatized with Cibacron Blue 3GA, as a separation matrix. Both of these technologies are based on in situ UV photopolymerization of glycidyl methacrylate (GMA) and trimethylolpropane trimethacrylate (TRIM) in channels.

• Elastomers and Composites
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• 제33권1호
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• pp.37-43
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• 1998

The permeation of gas through polymer membrane at temperatures above its glass transition, generally occurs by a solution-diffusion mechanism. This mechanism is performed by the affinity difference between polymeric materials and gas molecules, and various technologies, such as copolymerization, impregnation and so on, have been researched to improve the affinity of polymeric material for the gases. In this study, permeability and selectivity for some gases were obtained from steady-state rates of gas permeation through silicone rubber membrane which is prepared by supercritical fluid extraction method. The permeability was measured by the volumetric method proposed by Barrer. Permeability was increased generally with temperature and permeation pressure. Silicone rubber membrane shows a higher permeability to $CO_2$ than to $O_2$, $N_2$. This results probably reflect the relatively high solubility of CO_2 in silicone rubber membrane, which is due to the affinity of $CO_2$ molecules. Since separation powers of $CO_2/N_2$, $CO_2/O_2$ were more than 200, and 100, respectively, it is able to separate $CO_2$ from the air, and the optimum temperature and pres-sure was 328.15 K, 60 cmHg respectively. In future, it is possible that the silicone rubber membrane can be used for separation or concentration of $CO_2$ through experiment for mixed gas separation.

• Journal of Animal Science and Technology
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• 제47권6호
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• pp.1025-1032
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• 2005

난백 단백질 중 ovotransferrin을 gel chromato- graphy와 heparin affinity chromatography를 통하여 분리 하였다. 1차 gel filtration의 경우에 샘플인젝션 후 65-70min(fraction No. 14) 이후에는 ovalbumin이 ovotransferrin과 혼입되어 분리되고 오히려 ovalbumin의 농도가 더 높은 분포를 보였다. 고순도의 ovotransferrin을 분리하기 위하여 다시 fraction No. 12-14을 농축한 뒤 gel filtration을 실시한 결과 ovotransferrin이 완전히 분리되지 않았는데 이는 gel filtration만의 반복을 통해서 순수한 ovotransferrin을 얻는 것이 비효과적임을 의미하는 것으로 판단된다. Ovotransferrin을 heparin affinity chromatography을 이용하여 분리한 경우 칼럼에 Fe2+를 고정시킨 후 50mM EDTA를 흘려 주었는데 ovalbumin이 5-10분경에 용출이 되었고 10-15분경에 ovalbumin과 ovotransferrin이 같이 용출되었다. 그 후에 50mM Phosphate buffer (pH 7.2, 0.15M salt)를 흘려주었는데 여전히 ovalbumin의 밴드가 보여 순수하지 않음을 확인하였다. Fe3+를 컬럼에 고정시킨 후 50mM EDTA를 흘려주었을 때 ovalbumin이 10-15분경에 용출이 되었고 15-20분경에 ovalbumin과 ovotransferrin이 같이 용출되었지만 50mM Phosphate buffer (pH 7.2, 0.15M salt free)를 흘려주었을 때 156-165분경에 ovalbumin이 혼입되지 않은 매우 순수한 ovotransferrin이 용출되는 것을 확인하였다. 위의 결과를 종합해볼 때 gel chromatography를 반복적으로 실시한 경우 보다는 heparin affinity chromatography를 이용하여 분리하고 컬럼에 Fe2+를 고정시킨 경우보다 Fe3+를 고정시켰을 때 더욱 순수한 ovotransferrin을 분리해낼 수 있었다.