• Journal of Food Hygiene and Safety
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• v.25 no.4
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• pp.388-394
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• 2010

Metabolomics aims at the comprehensive, qualitative and quantitative analysis of wide arrays of endogenous metabolites in biological samples. It has shown particular promise in the area of toxicology and drug development, functional genomics, system biology and clinical diagnosis. In this study, analytical technique of MS instrument with high resolution mass measurement, such as time-of-flight (TOF) was validated for the purpose of investigation of amino acids, sugars and fatty acids. Rat urine and serum samples were extracted by selected each solvent (50% acetonitrile, 100% acetonitrile, acetone, methanol, water, ether) extraction method. We determined the optimized liquid chromatography/time-of-flight mass spectrometry (LC/TOFMS) system and selected appropriated columns, mobile phases, fragment energy and collision energy, which could search 17 metabolites. The spectral data collected from LC/TOFMS were tested by ANOVA. Obtained with the use of LC/TOFMS technique, our results indicated that (1) MS and MS/MS parameters were optimized and most abundant product ion of each metabolite were selected to be monitorized; (2) with design of experiment analysis, methanol yielded the optimal extraction efficiency. Therefore, the results of this study are expected to be useful in the endogenous metabolite fields according to validated SOP for endogenous amino acids, sugars and fatty acids.

• 한국초전도학회:학술대회논문집
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• 2009.07a
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• pp.106-106
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• 2009

• The KSFM Journal of Fluid Machinery
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• v.15 no.6
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• pp.11-17
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• 2012

The one way fluid structure interaction analysis on advanced propeller blade for next generation turboprop aircraft. HS1 airfoil series are selected as a advanced propeller blade airfoil. Adkins method is used for aerodynamic design and performance analysis with respect to the design point. Adkins method is based on the vortex-blade element theory which design the propeller to satisfy the condition for minimum energy loss. propeller geometry is generated by varying chord length and pitch angle at design point. Blade sweep is designed based on the design mach number and target propulsion efficiency. The aerodynamic characteristics of the designed Advanced propeller were verified by CFD(Computational Fluid Dynamic) and showed the enhanced performance than the conventional propeller. The skin-foam sandwich structural type is adopted for blade. The high stiffness, strength carbon/epoxy composite material is used for the skin and PMI(Polymethacrylimide) is used for the foam. Aerodynamic load is calculated by computational fluid dynamics. Linear static stress analysis is performed by finite element analysis code MSC.NASTRAN in order to investigate the structural safety. The result of structural analysis showed that the design has sufficient structural safety. It was concluded that structural safety assessment should incorporate the off-design points.

• Journal of Powder Materials
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• v.28 no.3
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• pp.221-226
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• 2021

We fabricate the non-equiatomic high-entropy alloy (NE-HEA) Fe_49.5Mn₃₀Co₁₀Cr₁₀C_0.5 (at.%) using spark plasma sintering under various sintering conditions. Each elemental pure powder is milled by high-energy ball milling to prepare NE-HEA powder. The microstructure and mechanical properties of the sintered samples are investigated using various methods. We use the X-ray diffraction (XRD) method to investigate the microstructural characteristics. Quantitative phase analysis is performed by direct comparison of the XRD results. A tensile test is used to compare the mechanical properties of small samples. Next, electron backscatter diffraction analysis is performed to analyze the phase fraction, and the results are compared to those of XRD analysis. By combining different sintering durations and temperature conditions, we attempt to identify suitable spark plasma sintering conditions that yield mechanical properties comparable with previously reported values. The samples sintered at 900 and 1000℃ with no holding time have a tensile strength of over 1000 MPa.

• Journal of the Ergonomics Society of Korea
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• v.32 no.1
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• pp.87-96
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• 2013

Objective: The aim of this study is to describe several issues which should be considered in the human reliability analysis of soft control operations in nuclear power plants. Background: The operational environment of advanced main control rooms is totally different from that of conventional control rooms. The soft control is one of the major distinguishable features of the advanced main control rooms. The soft control operations should be analyzed to estimate the effects on human reliability. Method: The literatures, about task analysis, simulation data analysis, and a human reliability analysis method for the soft control, were reviewed. From the review, important issues for the human reliability analysis of the soft control were raised. Results: The results of task and simulation data analysis showed that the soft control characteristics could have large effect on human reliability and they should be considered in the human reliability analysis of the soft control operations. Conclusion: The soft control may affect human error and performance of operators. The issues described in this paper should be considered in the human reliability method for the advanced main control rooms. Application: The results of the soft control operation analysis might help to design more efficient interface and education/training program for preventing human errors. The described issues might help to develop a human reliability analysis method for soft control operations.

• Proceedings of the Korean Vacuum Society Conference
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• 2004.08a
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• pp.229-229
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• 2004

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.5
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• pp.425-431
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• 2005

The systems-level analysis of microbes with myriad of heterologous data generated by omics technologies has been applied to improve our understanding of cellular function and physiology and consequently to enhance production of various bioproducts. At the heart of this revolution resides in silico genome-scale metabolic model, In order to fully exploit the power of genome-scale model, a systematic approach employing user-friendly software is required. Metabolic flux analysis of genome-scale metabolic network is becoming widely employed to quantify the flux distribution and validate model-driven hypotheses. Here we describe the development of an upgraded MetaFluxNet which allows (1) construction of metabolic models connected to metabolic databases, (2) calculation of fluxes by metabolic flux analysis, (3) comparative flux analysis with flux-profile visualization, (4) the use of metabolic flux analysis markup language to enable models to be exchanged efficiently, and (5) the exporting of data from constraints-based flux analysis into various formats. MetaFluxNet also allows cellular physiology to be predicted and strategies for strain improvement to be developed from genome-based information on flux distributions. This integrated software environment promises to enhance our understanding on metabolic network at a whole organism level and to establish novel strategies for improving the properties of organisms for various biotechnological applications.

• Natural Product Sciences
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• v.28 no.4
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• pp.168-180
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• 2022

Ephedra is a genus of the Ephedraceae family and is found in temperate regions, such as Central Asia and Europe. Among the various ephedra species, Ma Huang (Ephedra herb) is derived from the aerial parts of Ephedra sinica S tapf, Ephedra equisetina Bunge, and Ephedra intermedia Schrenk & C.A. Mey. Ma Huang contains various ephedra alkaloids, including (-)-ephedrine, (+)-pseudoephedrine, (-)-norephedrine, (+)-norpseudoephedrine, (-)-methylephedrine, and (+)-methylpseudoephedrine, which are found naturally as single enantiomers, although they can be prepared as racemates. Although the use of Ma Huang in foods is prohibited in Korea, products containing Ma Huang can be imported, and so it is necessary to develop a suitable analytical technique for the detection of Ma Huang in foods. Herein, we report the development of analytical methods for the detection of ephedra alkaloids in products containing Ma Huang. Following sample purification by solid phase extraction, quantitative analysis was performed using ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS). Additionally, the enantiomers were successfully separated using HPLC-DAD. We successfully analyzed various food samples, where the ephedra alkaloids were qualitatively and quantitatively determined, and the enantiomers were separated. It is expected that these methods may contribute toward preventing the distribution of illegal products containing Ma Huang.

• Analytical Science and Technology
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• v.36 no.1
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• pp.22-31
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• 2023

As the use of e-liquid cigarettes is rapidly increasing worldwide, it multiplies the potential risk undisclosed to the health of non- and smokers. To reduce the hazard, each country has its own set of regulations for controlling e-liquids. In Korea, the narrow definition of tobacco makes it difficult and have been steadily occurring tax evasion exploiting the difference in natural and artificial nicotine. Therefore, it is very important to distinguish source of nicotine for their regulation. To find biochemical discriminant markers, this study established analysis methods based on high-performance liquid chromatography coupled with diode array detector (HPLC-DAD) and high-performance liquid chromatography coupled with triple Quadrupole mass spectrometry (HPLC-MS/MS) for nicotine enantiomers and tobacco alkaloids targeted using the difference in pathways of nicotine biosynthesis and chemical synthesis. The method was validated by experimenting linearity (R² > 0.999), recovery (80.99-108.41 %), accuracy (94.11-109.73 %) and precision (0.04-8.27 %). Then, the results for discrimination of the nicotine obtained from analysis of 65 commercial e-liquid products available in Korean market was evaluated. The method successfully applied to the e-liquids and one sample labelled 'synthetic nicotine' for tax exemption was found to contain a natural nicotine product. This method can be used to determine whether an e-liquid product uses natural or artificial nicotine and monitor non-taxable e-liquid products. The method is more scientific than the existing one, which relies only on field evidence.

• 제어로봇시스템학회:학술대회논문집
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• 2003.10a
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• pp.2010-2015
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• 2003

In this paper, a design and control of the safe arm with passive compliant joints(PCJ) is presented. Each PCJ has a magneto- rheological damper and maximum 6 springs. Compliance analysis in Cartesian space is performed with the compliance ellipsoid; this analysis shows a map between compliance in the joint space and compliance in Cartesian space. Vibration control of the arm using an input shaping technique is also presented; the results of a simulation and an experiment prove that a fast motion of the safe arm without residual vibration can be performed.