• Parasites, Hosts and Diseases
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• 제41권2호
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• pp.135-137
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• 2003

The carbohydrate moieties of larval sparganum proteins in two different species, the snakes, Elaphe rufodorsata, the Balb/c mouse and those of the adult worm, Spirometra erinacei, were compared using five different lectins including GNA, SNA, MAA, PNA and DSA. The GNA positive 53 kDa molecule, which is excretory-secretory protease in the sparganum from the snake showed a stage specific and developmental regulation. We also suggested that sparganum glycosylation may be involved in immune evasion and differentiation into an adult worm.

• Parasites, Hosts and Diseases
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• 제51권2호
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• pp.177-182
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• 2013

The purpose of the present study was to evaluate the potential role of the 27-Kilodalton (KDa) antigen versus Fasciola gigantica adult worm regurge antigens in a DOT-Blot assay and to assess this assay as a practical tool for diagnosis fascioliasis in Egyptian patients. Fasciola gigantica antigen of an approximate molecular mass 27- (KDa) was obtained from adult worms by a simple elution SDS-PAGE. A Dot-Blot was developed comparatively to adult worm regurge antigens for the detection of specific antibodies from patients infected with F. gigantica in Egypt. Control sera were obtained from patients with other parasitic infections and healthy volunteers to assess the test and compare between the antigens. The sensitivity, specificity, positive and negative predictive values of Dot-Blot using the adult worm regurge were 80%, 90%, 94.1%, and 69.2% respectively, while those using 27-KDa were 100% which confirms the diagnostic potential of this antigen. All patients infected with Fasciola were positive, with cross reactivity reported with Schistosoma mansoni serum samples. This 27-KDa Dot-Blot assay showed to be a promising test which can be used for serodiagnosis of fascioliasis in Egyptian patients especially, those presenting with hepatic disease. It is specific, sensitive and easy to perform method for the rapid diagnosis particularly when more complex laboratory tests are unavailable.

• Applied Microscopy
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• 제12권2호
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• pp.35-44
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• 1982

The fat bodies of cabbage worm (Pieris rapae) and silk worm (Bomyx mori) during metamorphosis was comparatively studied by electron microscope. 1. Cell oranelles: Golgi apparatus were not observed in both species. It is observed that RER of cabbage worms initiate to degenerate in prepupa stage with complete degeneration at adult stage, while that of silk worms shows similar degenerative pattern. However, mitochondria of cabbage worms are transformed into autophagic vacuole from prepupa stage until adult stage whereas those of silk worm shows a decrease in number in prepupa stage but maintains a certain level until adult stage. 2. Storage substance in cell: Lipid droplets in cabbage worms were observed to increase in numbers during larval stage but afterward decrease in number with an enlargement in size. However immediately after their pupal stage, they almost disappear. On the contrary lipid droplets in silk worms show rather increase in number until adult stage. Protein storage granules in bothspecies were arised from autophagic vacuoles(lysosome) . Fat cells of cabbage worm in adult stage turn out to be residual bodies which last until final stage, but those of silk worm rapidly decrease. Glycogen particles in both species reach maximum at last larval instar and thee gradually decrease thereafter. 3. Fat body sheath: The average width of fat body sheath was measured to be $0.2{\mu}m$ and $0.6{\mu}m$ and surface of fat cells adjacent to fat body sheath in silk worm is heavily infolded.

• Parasites, Hosts and Diseases
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• 제53권6호
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• pp.725-730
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• 2015

We report here an ectopic case of Fasciola hepatica infection confirmed by recovery of an adult worm in the mesocolon. A 56-year-old female was admitted to our hospital with discomfort and pain in the left lower quadrant of the abdomen. Abdominal CT showed 3 abscesses in the left upper quadrant, mesentery, and pelvic cavity. On surgical exploration, abscess pockets were found in the mesocolon of the sigmoid colon and transverse colon. A leaf-like worm found in the abscess pocket of the mesocolon of the left colon was diagnosed as an adult fluke of F. hepatica. Histologically, numerous eggs of F. hepatica were noted with acute and chronic granulomatous inflammations in the subserosa and pericolic adipose tissues. Conclusively, a rare case of ectopic fascioliasis has been confirmed in this study by the adult worm recovery of F. hepatica in the mesocolon.

• Parasites, Hosts and Diseases
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• 제54권1호
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• pp.47-53
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• 2016

Echinostomes are intestinal trematodes that infect a wide range of vertebrate hosts, including humans, in their adult stage and also parasitize numerous invertebrate and cold-blooded vertebrate hosts in their larval stages. The purpose of this study was to compare Echinostoma malayanum parasite growth, including worm recovery, body size of adult worms, eggs per worm, eggs per gram of feces, and pathological changes in the small intestine of experimental animals. In this study, 6-8-week-old male hamsters, rats, mice, and gerbils were infected with echinostome metacercariae and then sacrificed at day 60 post-infection. The small intestine and feces of each infected animal were collected and then processed for analysis. The results showed that worm recovery, eggs per worm, and eggs per gram of feces from all infected hamsters were higher compared with infected rats and mice. However, in infected gerbils, no parasites were observed in the small intestine, and there were no parasite eggs in the feces. The volume of eggs per gram of feces and eggs per worm were related to parasite size. The results of histopathological changes in the small intestine of infected groups showed abnormal villi and goblet cells, as evidenced by short villi and an increase in the number and size of goblet cells compared with the normal control group.

• Parasites, Hosts and Diseases
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• 제29권1호
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• pp.31-42
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• 1991

폐흡충(Paragonimus westermani)에 감염된 숙주에 대해 항체 생성을 유도하는 물질이 분포하고 있는 부위를 화인하기 위하여 대조군의 IgG와 폐홉충에 감염된 실험 개의 특이 IgG를 폐흡충 유약성충 조직에 반응시키고 면역황금표식법을 이용하여 전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 대조군의 IgG를 반응시킨 유약성충 표피층은 표피세포에서 분비되는 물질이 포함된 표피 합포체로 구성되어 기저층과 분명하게 구별되었으며 근육층도 잘 발달되어 있었다. 난황세포는 크기가 다양한 분비과립 등을 포함하고 있었으며 특히 조면소포체가 발달되어 있었다. 맹관의 막구조물은 잘 발달되었으며 장상피 합포체도 잘 발달되어 있었다. 한펀, 폐흡충에 감염된 실험동물의 특이 항체로 반응시킨 유약성충 표피충의 표피 합포체와 표피세포의 세포질에 황금입자의 매우 특이적인 표식가 관찰되었고 난황세포에서는 분필과립에 황금 입자가 높은 밀도로 관찰되었다. 그리고 맹관 상피 합포체는 미약하게 황금 표지가 되었고 맹관 막구조물과 맹관 내강에도 특이하게 황금 표지가 관찰되었지만 표피 합포체와 표피세포보다 밀도가 낮게 관찰되었다. 이상의 결과로 폐홉충에 감염된 실험 개는 폐흡충의 표피 합포체와 표피세포에서 생성된 물질과 난황세포에서 생성된 물질에 의하여 면역항체가 형성되며 일부는 맹관 내용물에 의해 약간의 면역항체가 유도되는 것으로 생각되었다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2004년도 ICEIC The International Conference on Electronics Informations and Communications
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• pp.417-420
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• 2004

Caenorhabditis (C) elegans is often used in genetic analysis in neuroscience because its simple organism; an adult hermaphrodite contains only 302 neuron. So the worm is often used to study of cancer, alzheimer disease, aging, etc. To analysis mutant type of the worm, an experienced observer was able to subjectively before, but requirements for objective analysis are now increasing. For this reason, we use automated tracking systems to extract global movement coordinate of the worm. In this paper, we extract features, which are related on reversal and movement of the worm. Using these features, we quantitatively analysis 6 type mutant by movement and reversal characteristic.

• Parasites, Hosts and Diseases
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• 제47권3호
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• pp.315-318
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• 2009

Quantitative fecal egg counts represented as the number of eggs per gram of feces (EPG) are generally a reliable parameter to estimate the worm burden of intestinal and hepatic parasitoses. Although Haplorchis taichui (Digenea: Heterophyidae) is one of the most common minute human intestinal flukes, little is known about the relationship between EPG and the actual worm burden in patients or the severity of the disease. In the present study, fecal samples were collected from 25 villagers in northern Thailand before and after praziquantel treatment. The EPG values of each participant were determined by the modified cellophane thick smear method, and adult worms were collected from the whole stool after the treatment. Eggs per day per worm (EPDPW) of H. taichui were estimated 82 from egg counts and expelled worms. The EPG was not well correlated with the worm burden, and a reverse correlation was observed between the EPDPW and the worm burden.

• Parasites, Hosts and Diseases
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• 제50권2호
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• pp.143-146
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• 2012

Diphyllobothrium latum infections in 4 young Korean men detected from 2008 to 2012 are presented. Three were diagnosed based on spontaneously discharged strobila of the adult worm in their feces, and 1 case was diagnosed by finding the worm at colonoscopy examination in a local clinic. The morphologic characteristics of the gravid proglottid and eggs were consistent with D. latum. All patients were treated with praziquantel 15 mg/kg, and follow-up stool examinations were done at 2 months after the medication. The main clinical complaints were intermittent gastrointestinal troubles such as indigestion, abdominal distension, and spontaneous discharge of tapeworm's segments in their feces. The most probable source of infection was the flesh of salmon or trout according to a patient's past history. These are the 45th to 48th recorded cases diagnosed by the adult worm in the Republic of Korea since 1971.

• 대한수의학회지
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• 제42권1호
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• pp.109-113
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• 2002

This is the first case report of Stephanurus dentatus infection of a feral pig in Korea. In late April, 2000, a weakened feral pig was caught by blow gun from a very low level mountain near the Gyeongsang National University. We autopsied the feral pig in the laboratory of veterinary anatomy at the College of Veterinary Medicine. A total of 27 adult parasites, 11 females and 16 males, and numerous eggs were observed from the cysts formed in the perirenal tissues and ureters. The average size of males was $25.1{\pm}3.2mm$ long and of the females was $34.2{\pm}2.9mm$. The worms were stout, the females being about 2mm broad, and the internal organs were partly visible through the cuticle. The shape of thin-shelled eggs found in the cysts of perirenal tissues and ureter was ellisoidal and oval, and measured $40{\sim}65{\times}90{\sim}115{\mu}m$. The adult parasites were found in cysts which varied from 0.6 to 4cm in diameter, each cyst usually containing a pair of adult worms embedded in green pus. The ureter was thickened and almost occluded, with consequent hydronephrosis.