• BMB Reports
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• 제29권5호
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• pp.474-480
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• 1996

We present a study of evolutionary changes in expression of actin genes among closely related sea urchin species that exhibit different modes of early development. For this purpose, polyclonal antisera raised against peptides from the carboxyl terminus of the HeCyI cytoskeletal actin of Heliocidaris erythrogramma were used. H. erythrogramma is a direct developing sea urchin that proceeds from embryonic to adult stages without an intervening feeding larval stage. Expression patterns of the CyI actin isoform were compared with those of Heliocidaris tuberculata and to a related sea urchin Strongylocentrotus purpuratus, which both produce a feeding pluteus larval stage. The CyI actin of all three species is expressed in the same cell types. However, its expression patterns have been changed with reorganization of early cell lineage differentiation, which is apparent among the three species. Thus. evolutionary changes in CyI actin gene expression patterns are correlated with not only phylogenetic relationship, but developmental mode. The implication of this observation is that evolutionary changes in expression patterns of histospecific genes may underlie the emergence of novel developmental processes.

• Parasites, Hosts and Diseases
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• 제53권3호
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• pp.329-333
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• 2015

The emergence of dirofilarial infections in Asia including Vietnam is a clinically significant threat to the community. We here report a rare case of subcutaneous Dirofilaria repens infection on the posterior thoracic wall in a young woman presenting a painful, itchy, and palpable nodule. The adult worm was identified by mitochondrial cox1 and nuclear ITS-2 sequence determination. The diagnosis was additionally confirmed by 16S rRNA sequencing of the endosymbiont Wolbachia pipientis commonly co-existing with D. repens. This is a rare case of subcutaneous human infection on the posterior thoracic region caused by D. repens.

• International Journal of Industrial Entomology and Biomaterials
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• 제5권1호
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• pp.33-36
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• 2002

Mulberry leaf roller, Diaphania pulverulentalis (Hampson), is a major pest of mulberry, Morus alba. The life table construction from present investigation reveals the age specific survival $(1_{x)}$ and the age specific fecundity $(m_{x})$ of the pest. The female contributed the highest egg production $(m_{x} ＝ 12.75)4 in the life cycle on the $27^{th}$ day and the lowest $(m_{x} = 0.8)$ on the 37$^{th}$ day of the pivotal age. The female progeny production was observed to be intensive for the first six days after the preoviposition period, beyond which it declines steadily, The fecundity of D. pulverulentalis varies between 60-140. The first female mortality within the cohort occurred 4 days after the adult emergence and mortality increased thereafter, One generation is completed in 33.08 days. The female dominated sex ratio (1: 7.18 days) was recorded.

• Environmental Analysis Health and Toxicology
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• 제23권4호
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• pp.277-284
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• 2008

The ecotoxicological effects of 4-tert-octylphenol were observed on non-biting midge, Chironomus plumosus collected from Anyang stream in Seoul. The survival rate and adult emergence rate on C. plumosus exposed to octylphenol were not significantly affected. However, 4-tert-octylphenol induced developmental delay and disrupted sex ratio in high concentration of octylphenol. The mouth deformity such as tooth deletion or fusion in mentum, and tooth deletion in mandible were observed exposure to 4-tert-octylphenol. The deformity type of the mentum showed deletion (LT, 6.7%), and fusion (LT, 6.3%). Moreover, tooth deletion of mandible was observed in 4-tert-octylphenol treated groups (6.7%, 3 ppm).

• International Journal of Industrial Entomology and Biomaterials
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• 제1권1호
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• pp.47-52
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• 2000

The effect of supplementation with potassium nitrate on economic parameters was analysed following treatment of last larval stadium. The treated larvae showed a significant increase in larval weight at 500$\mu\textrm{g}$/ml and silk gland weight in all the treated groups along with other enhanced larval, cocoon and adult parameters. The larval period was significantly decreased in all the treated groups with increased female and male cocoon weights its shell weights filament lengths at 500$\mu\textrm{g}$/ml. The denier was significantly decreased in all the groups. The filament weight moth emergence percentaged length of the ovariole were unaffected. However, eggs per ovariole, fecundity and hatching percentage significantly decreased compared with the corresponding parameters of the carrier control.

• International Journal of Industrial Entomology and Biomaterials
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• 제1권1호
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• pp.41-45
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• 2000

The effect of topical application with hydrocortisone on economic parameters was analysed following treatment of last larval stadium. The treated larvae showed a significant increase in larval weight at higher concentrations along with other enhanced larval, cocoon and adult parameters. This suggests that hydrocortisone can be used effectively for commercial silkworm rearing. The larvas period was significantly increased in all the treated groups with increased female cocoon weights, its shell weights and male cocoon shell weight at 20$\mu\textrm{g}$/ml treated group. Filament length, weight and denier were increased significantly in all the treated groups. Moth emergence percentage, length of the ovariole, eggs per ovariole, fecundity and rind hatching percentage were unaffected when compared with that of the carrier control group. This suggests that hydrocortisone in addition to affecting larval growths also affect silk crop.

• Educational Technology International
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• 제6권2호
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• pp.11-21
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• 2005

The study analyzed the topics and the types of the selected articles from the 20-year-old Journal of Educational Technology(JET), flagship journal of the Korean Society of Educational Technology. Further the results were compared with those of the Educational Technology Research & Development journal. Results indicate that the JET has grown in terms of quantity and quality since its birth 20 years ago. The JET seems to be independent as a young adult. Some issues and concerns were also identified: The articles of the JET are more inclined to Experimental design in terms of research type, and to Design in terms of research topic. Balance across Types and Topics needs be kept for long-term, healthier growth. The emergence of e-Learning and high technology of Korea offered opportunities to the field. However, the monopoly of e-Learning that consumes most of the limited space of the JET raises a red flag. More balanced and quality-oriented endeavors were suggested for another 20 years coming.

• 한국응용곤충학회지
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• 제41권2호
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• pp.123-128
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• 2002

벼물바구미의 생식과 발육에 미치는 온도의 영향을 2$0^{\circ}C$에서 3$0^{\circ}C$사이의 온도에서 수행하였다. 20, 25, 3$0^{\circ}C$에서 산란전기간은 5월에 채집한 월동성충보다 3월에 채집한 충에서 길었다. 그러나 같은 온도조건에서 산란기간은 3월에 채집한 월동성충보다 5월에 채집된 충이 길었다. (16.9일, 22.0일).산란기간이 긴 5월 채집충이 산란수도 많은 결과를 가져왔다. 난기간은 15.1일(2$0^{\circ}C$)에서 4.5일(3$0^{\circ}C$)로 온도가 높을수록 짧았고, 그리고 부화율은 27$^{\circ}C$에서(100%) 가장 높았다. 난부터 성충까지의 발육기간은 온도가 높아질수록 짧았다; 2$0^{\circ}C$에서 77.9일, 3$0^{\circ}C$에서 38.3일이었다. 난 발육영점온도(T)와 유효적산온도(K)는 각각 16.3$^{\circ}C$와 62.1일도이었다. 난에서 성충우화까지는 각각 13.9$^{\circ}C$와 577.6일도이었다. 제1세대 성충은 2$0^{\circ}C$에서 산란하지 못하였고, $25^{\circ}C$와 3$0^{\circ}C$에서는 산란하였다. 내적자연증가율(r$_{m}$ )은 온도가 높을수록 증가되었다. 1세대당 순증식율(Ro)은 $25^{\circ}C$에서 가장 높았다(75.3).

• 생명과학회지
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• 제30권4호
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• pp.386-393
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• 2020

최근, 환경오염의 심화, 식량·에너지 부족, 지구온난화 문제에 대한 대안으로 곤충산업이 주목받고 있다. 본 연구에서는 환경정화 곤충 및 사료용 곤충으로 각광받고 있는 동애등에(Black soldier fly, Hermetia illicens)의 고부가 가치화를 위해, 생육시기별로 유충(2령~6령), 번데기, 용각 및 성충을 회수하여 이의 에탄올 추출물을 조제하고, 이의 유용성분 함량, 혈액응고저해 및 혈소판 응집저해 활성을 평가하였다. 동애등에 유충은 15일 사육(2령~6령) 동안 25배 무게가 증가하였으며, 흰색-미색-흑갈색으로 변화하였으며, 에탄올 추출수율은 생육시기에 따라 1.0~18.5%로 다양하였다. 추출물의 총 폴리페놀 함량은 용각(17.2 mg/g)에서 가장 높았으며, 번데기, 성충, 2령 순으로 나타났으며, 총 플라보노이드 함량은 번데기(3.4 mg/g), 용각, 6령 순으로 나타났다. 반면 총당 함량은 6령(37.6 mg/g)에서 특이하게 높게 나타났으며, 용각, 번데기, 5령순으로 나타났다. 혈액응고 저해 활성 평가결과, 용각 추출물에서 가장 우수한 트롬빈저해, 내인성 및 외인성 응고 저해를 나타내었으며, 6령 및 번데기 추출물에서도 우수한 항응고 활성을 확인하였다. 혈소판 응집저해 활성 평가에서는 3령, 용각 및 성충 추출물에서 우수한 응집저해능이 나타났으며, 4령 및 5령 추출물은 오히려 혈소판 응집을 촉진함을 확인하였다. 현재까지 동애등에의 항혈전 활성은 보고된 바 없으며, 특히 생육시기별 활성 평가도 보고된 바 없다. 본 연구결과는 동애등애 번데기 및 용각을 이용한 항혈전 소재 개발이 가능함을 제시하고 있다.

• 한국응용곤충학회지
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• 제40권4호
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• pp.297-300
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• 2001

감꼭지나방의 온도별(15, 20, 25, 3$0^{\circ}C$)발육과 생식에 미치는 영향을 조사하였다. 알에서 성충까지의 발육기간은 2$0^{\circ}C$에서 96.1일, $25^{\circ}C$에서 43.2일, 3$0^{\circ}C$에서 34.6일이 걸렸다. 15$^{\circ}C$에서는 유충기간중 모두 사망하였다. 발육영점온도는 알, 유충, 번데기, 알~성충이 각각 12.2, 13.5, 13.8, 13.4$^{\circ}C$였다. 유효적산온도는 알, 유충, 번데기, 알~성충이 각각 74.0, 331.3, 160.5, 569.9일도 이었다. 부화율, 용화율 및 우화율은 $25^{\circ}C$에서 가장 높았다. 산란전 기간과 성충수명은 2$0^{\circ}C$에서 12.6일과 29.3일이며, $25^{\circ}C$에서는 3.8일과 8.6일, 3$0^{\circ}C$에서 2.5일과 7.0일이었다. 1세대에 요하는 평균기간(T)은 온도가 높아질수록 짧았으며, 순증식률(R$_{o}$ )은 2$0^{\circ}C$와 3$0^{\circ}C$에서 낮았다. 내적자연증가율(r$_{m}$ )은 $25^{\circ}C$에서 0.066로 가장 크게 나타내었다.