• 한국수정란이식학회지
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• 제31권3호
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• pp.293-297
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• 2016

For evaluating the boar semen quality, sperm motility is an important parameter because the movement of sperm indicates active metabolism, membrane integrity and fertilizing capacity. Phospholipase C zeta (PLCz) is important enzyme in spermatogenesis, but the effect has not been confirmed in pigs yet. Therefore, this study was aimed to analyze their association with sperm motility and kinematic characteristics. DNA samples from 124 Duroc pigs with records of sperm motility and kinematic characteristics [total motile spermatozoa (MOT), curvilinear velocity (VCL), straight-line velocity (VSL), the ratio between VSL and VCL (LIN), amplitude of lateral head displacement (ALH)] were subjected. A SNP in non-coding region of PLCz g.158 A > C was associated with MOT (p < 0.05), VCL (p < 0.01), LIN (p < 0.01) and ALH (p < 0.05) in Duroc population. Therefore, we suggest that the intron region of the porcine PLCz gene may be used as a molecular marker for Duroc boar semen quality, although its functional effect was not defined yet. Whether the association is due to the candidate gene or not require further verification. Thus, it will be of interest to continue association studies in the regions surrounding those genes.

• 한국수정란이식학회지
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• 제31권3호
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• pp.281-285
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• 2016

Cluster-of-differentiation antigen 9 (CD9) gene expressed in the male germ line stem cells is crucial for sperm-egg fusion, and was therefore selected as a candidate gene to investigate Duroc boar semen motility and kinematic characteristics. This study was performed to investigatetheir association with semen motility and kinematic characteristics. DNA samples from 96 Duroc pigs with records of sperm motility and kinematic characteristics [Total motile spermatozoa (MOT, $82.27{\pm}5.58$), Curvilinear velocity(VCL, $68.37{\pm}14.58$), Straight-line velocity(VSL, $29.06{\pm}6.58$), the ratio between VSL and VCL(LIN, $47.36{\pm}8.42$), Amplitude of Lateral Head displacement(ALH, $2.88{\pm}0.70$)] were used in present study. A single nucleotide polymorphism (g.358A>T) in intron 6 was associated with MOT, VCL, VAP and ALH in Duroc population (p<0.05). Therefore, we suggest that the porcine CD9 may be used as a molecular marker for Duroc boar semen quality, although its functional effect was not clear yet. These results will improve the understanding of the functions of the CD9 in spermatogenesis within the reproductive tracts, and will shed light on CD9 as a candidate gene in the selection of good sperm quality boars.

• 한국수정란이식학회지
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• 제31권3호
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• pp.215-219
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• 2016

Value of excellent breeding animals is important in livestock industry, but their economic life time is limited. And, many countries have been trying procuration of genetic resource in good animals. Therefore, this study was conducted to determine embryo production and to test efficiency of embryo transfer via non-surgical artificial insemination (AI) in different breed of superior sows. A total of 17 sows were used in this experiment (Duroc, n=10; Landrace, n=4; Yorkshire, n=3). The sows were artificially inseminated by semen of same breed boars. After 4 or 5 days following the AI, the embryos were obtained from the sows and then transferred to Landrace and Yorkshire recipients (n=3, respectively) by non-surgical method. The corpora lutea tended to be increased in Yorkshire and Landrace than Duroc(28 and 26 vs. 17, respectively). The recovery of embryo was 78.8% in Landrace, 65.4% in Duroc and 51.4% in Yorkshire. Duroc showed lower morulaes and early blastocyst embryos than 2, 4, 8 and 16 cell. The morula in Yorkshire was higher (P<0.05) than that of Duroc (4.7 vs. 3.4). Similarly, the morulaes and early blastocyst embryos presented greater (P<0.05) in Landrace compared with other breed sows. The recipient sows were pregnant in a Landrace only. This reason may be due to little embryos inserted in the recipients. In addition, pregnancy results were limited because of the little sows. In conclusion, ovulated ovum in sows can be affected by different breed. Also, further study needed pregnant test by using the many embryo in each breed.

• 한국환경농학회지
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• 제35권3호
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• pp.202-210
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• 2016

BACKGROUND: This study was conducted to determine effects of ridging systems and plastic film mulch types on growth, yield, and profitability of potato (Solanum tuberosum L.) in spring cropping using paddy field and to suggest profitable ridging system and plastic mulch type.METHODS AND RESULTS: Two potato cultivars ('Goun', and 'Atlantic') were grown at paddy field located in Gangneung in spring. For treatments, ridging systems were one-row ridge (OR) and two-row ridge (TR). Mulch types were no-mulch (NM), black plastic film mulch (BPM), and transparent plastic film mulch (TPM). Emergence of sprout was affected by the mulch types and the fastest level was shown at TPM treatment. Accumulative soil temperature during sprouting was 16.2℃ higher at TR than at OR and also higher at TPM than at BPM or NM. Stem length was not affected by ridging systems or mulch types. Leaf area index (LAI) was influenced by mulch types, while not by ridging systems. The highest LAI was shown at TR with TPM and OR of BPM. There was no significant difference in specific gravity or dry matter rate by ridging systems and mulch types. Tuber yield was significantly influenced by ridging systems and mulch types. Mean tuber weight was heavier at OR treatment than at TR and also the heaviest at TPM among mulch types. The highest marketable yield was found at OR with BPM. Based on marketable tuber yield and market price, the highest income ratio in two cultivars was found at OR with BPM and it was 20~82% higher than the ratio at TR with TPM.CONCLUSION: In spring potato cropping using paddy field, OR with BPM is better for high yield and is more profitable for farmer's income than the conventional cultivation method, TR with TPM.

• 한국작물학회지
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• 제50권spc1호
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• pp.94-98
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• 2005

경북 지역 농업기후 지대를 미질 및 식미치를 근거로 하여 다시 세분화된 지대를 설정하고자 2002년부터 2004년까지 3년간 경북 전 지역에서 가장 많이 재배되고 있는 품종 중 중만생종인 일품벼, 주남벼, 남평벼, 중생종인 화영벼, 조생종인 오대벼를 재배하고 있는 논 513개소의 쌀을 수집하여 분석 조사한 결과를 요약하면 다음과 같다. 1. 지대별 식미치의 평균은 동해안 남부지대가 67.6로 다른 지대에 비해 약간 낮았으며, 완전미 비율은 동해안 남부지대 및 동해안 중부지대가 각각 $87.3\%,\;88.2\%$로 낮았으며 변이계수도 각각 $8.2\~8.3\%$로 높았다. 단백질 함량은 동해안 중부지대가 $8.0\%$로 다른 지대에 비해 약간 높았고, 변이계수도 $8.8\%$ 높았다. 2. 특정지역을 세분하여 쌀 품질을 조사해보면 전체 평균의 변이계수보다 높게 나타나는 지역과 낮게 나타나는 지역으로 뚜렷하게 구분된다. 변이 계수가 크다는 것은 특정 지역 내에서도 쌀 품질의 세분화된 지역간 차이가 심하므로 고품질 쌀 생산을 위해서는 세분화된 벼 재배 구역 설정하여 적지적작의 정밀농업이 필요하였다. 3. 식미치를 근거로 세분화된 지대의 쌀 품질 요인에 대한 연차 간 변이계수는 기존 농업기후지대의 변이계수보다 상당히 낮아져, 세분화된 지대별 고품질 쌀 생산을 위한 특정 지역의 정밀 기술 개발 및 보급의 기초 자료로 활용할 수 있을 것으로 사료되었다.

• 대기
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• 제14권3호
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• pp.3-18
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• 2004

A heavy snowfall event occurred over the middle part of the Korean peninsula on March 4, 2004. The numerical models of KMA failed to forecast this heavy snowfall event because this event was due to small scale disturbance by low lever convergence and atmospheric instability. The analyses for this heavy snowfall have been performed to give forecasters useful suggestions for forecasting heavy snowfall events in the future. The analyses for the snowfall event were recounted by the Hourly Korean Peninsula Analysis Weather Chart (HKPAWC) presenting on the KMA intranet system. We confirmed that warm air flows of low level into south central Korea in conjunction with strong southwesterly winds played important role in the heavy snowfall event. We suggested several check points to improve the forecast of heavy snowfall events in the future through the results of the analyses.

• 대한한방내과학회지
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• 제19권2호
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• pp.219-232
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• 1998

Paljintanggagmbang has been used for cure of tumor as a traditional medicine without any experimental evidence to support the rational basis for its clinical use. This study was carried out to evaluate. the possible therapeutic or antitumoral effects of Paljintanggagmbang extract against tumor, and to carry out some mechanisms responsible for its effect. Experimental studis were performed for measurement of Humoral and Cellular Immune Response and Phagocytosis in Mice treated with mitomytion C(MMC) and Paljintanggagmbang alone and combination. The results obtained in this study were as follows 1. The adminstration of Paljintanggagmbang extract decresed size of tumors cell which MCA induced. 2. The adminstration of Paljintanggagmbang extract decresed growth of the tumors which S 180 transplant. 3. The adminstration of Paljintanggagmbang extract decresed reproduction of A549, Hep3b, 3LL cell and S 180 in vivo. 4. The adminstration of Paljintanggagmbang extract incresed activity of the NK cell. These results also suggested that effect of Paljintanggagmbang might be chiefly due to nonspecific enhancement of Humoral and Cellular Immune Response and Phagocytosis in Mice treated with MMC and Paljintanggagmbang alone and combination.

• 한국품질경영학회:학술대회논문집
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• 한국품질경영학회 2006년도 춘계학술대회
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• pp.54-65
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• 2006

Since the ending of the IMF period, the Korean health industry has experienced a number of changes in its environment such as hospital bankruptcy, enforcement of SDF(Separation of Dispensing and Prescription), opening of the medical market by WHO, evaluation of medical institutions with more than 100 sick beds, and limited approval for medical service advertisement. Furthermore, the concept of medical service has changed from a beneficent and vortical one to a hi lateral and righteous one. These changes in medical service have required medical institutions to provide customers with medical service options and adopt market principles actively, while considering customer satisfaction. Thus, this paper aims to investigate the service quality and service value of medical institutions, to understand the mutual relationship between customer satisfaction and repeat visits, and to suggest better solutions for the improvement of service quality. For future studies, It will be necessary to overcome the limitations of this research and develop proper measurement tools on service quality in the Korean medical system.

• 농업과학연구
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• 제42권4호
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• pp.389-396
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• 2015

This study was performed to investigate the opinions of various related-industries on ban of antibiotics growth promoters (AGPs) in commercial mixed feed. The answers on a total of 21 questions were summarized by response number and percentage. 93% of those surveyed were in agreement of a ban of dietary AGPs. The agreement reasons were the livestock safety (61.5%), the reduction of antibiotic use (23.1%), and decrease of antibiotic-resistant bacteria (11.5%). The negative effects expected by the ban of AGPs were poor growth performance (44.2%), elevated disease emergence (31.4%), increasing the feed cost (18.6%), and quality degradation of livestock (5.8%). As the efficient plans for decline of AGPs use, the feeding environment improvement was the highest with 43%, and farmer training and the consolidation inspection of residual substance on antibiotics in livestock product was 27.9% and 22.1%, respectively. 46.5% of respondent are considering the modification of feed spec and 39.5% of those surveyed have staged a modified feed spec. In conclusion, livestock related-industries approve a ban of AGPs, and they assert that the policy support, improvement of management and environment in the farm, providing technology from related-industries are multiply essential for a stable settlement of a ban policy of AGPs.

• 농업과학연구
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• 제42권4호
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• pp.355-359
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• 2015

The cause of infertility is either fertilization failure or early embryonic death. The aetiology may involve a combination of many factors, e.g. genetic factors, abnormalities in the gametes nutritional disorders, inadequate luteal function, and delayed ovulation. This study was conducted to investigate the effect of microorganisms collected from uterus of Hanwoo cattle on early embryonic development. Microorganisms isolated from the uterus of Hanwoo cattle included Bacillus cereus (Bc), Bacillus thuringiensis (Bt), Staphylococcus warneri (Sw) and Enterococcus faecalis (Ef). When cultured with Bc, Bt, Sw, and Ef, oocytes were not developed into a blastocyst in vitro. The proportion of blastocyst was dramatically increased after reducing the number of microorganisms ($10^4CFU/ml$). Interestingly, the proportion of blastocyst was decreased by adding the Sw and Ef. These results indicate that among intrauterine microorganisms, Sw and Ef strains negatively affect theearly embryonic development, thereby aggravate the rates of implantation and pregnancy. These findings will provide useful information for association studies in other pig populations.