• 제목/요약/키워드: adipocyte size

검색결과 122건 처리시간 0.028초

고지방 식이로 유도된 비만 생쥐에서 창출약침의 항비만 효과 (Anti-obesity Effect of Rhizoma Atractylodis Herbal Acupuncture in High Fat Diet-induced Obese ICR Mouse)

  • 유은주;서병관;남상수;강성길
    • Journal of Acupuncture Research
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    • 제27권6호
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    • pp.31-42
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    • 2010
  • Objectives : The aim of this study was to investigate the anti-obesity potential and mechanisms of action of Rhizoma Atractylodis(RA) herbal acupuncture in high fat diet- induced obese ICR mice. Methods : Sample solutions for herbal acupuncture were prepared from the Rhizoma Atractylodis water extract powder at concentration of 150mg/kg and 300mg/kg with distilled water. Five week-old ICR mice acclimatized to the laboratory environment for 1 week were allocated into four groups: regular diet group (RD), high fat diet group(HFD), groups fed HFD with 150mg/kg RA herbal acupuncture treatment (RAE 150) and with 300mg/kg RA herbal acupuncture treatment(RAE 300). Herbal acupuncture groups were injected with either 150mg/kg or 300mg/kg of Rhizoma Atractylodis(RA) subcutaneously onto both Sinsu($BL_{23}$) alternately on the same time everyday for 30days. Body weight, gross appearance of epididymal fat area, blood glucose, insulin, insulin resistance(HOMA-IR), non-esterified fatty acid, cholesterol, triglyceride, AST, ALT, histological analysis of white adipose tissue, gene expression responsible for adipocyte differentiation and AMPK activation were analyzed. Results : RA herbal acupuncture inhibited the development of weight gain, hyperglycemia, hyperinsulinemia, hyperlipidemia, increases of AST and ALT, and the enlargement of fat cell size induced by HFD. Also, RA herbal acupuncture inhibited the expression of PPAR-${\gamma}$, C/$EBP{\alpha}$, aP2, LPL, FAS, SCD-1 and enhanced the activation of AMP-activated protein kinase. Conclusions : The results of this study demonstrate that RA herbal acupuncture can exert the anti-obesity effect and it is partially mediated by activation of AMPK and inhibition of the gene expressions responsible for adipocyte differentiation. Further studies will be required to ascertain the nti-obesity effect and mechanisms of action of RA herbal acupuncture in animal models and human for aclinical application.

순기제습방(順氣除濕方)이 고지방식이(高脂肪食餌)로 유발된 비만(肥滿) 백서(白鼠)에 미치는 영향 (The Inhibitory Effects of Soongijeseub-bang(Shunqichushi-fang) on the Obese-mice Caused by High Fat Diet)

  • 이우열;윤일지
    • 한방재활의학과학회지
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    • 제18권3호
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    • pp.51-65
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    • 2008
  • Objectives : In order to investigate the effects of Soongijeseub-bang(Shunqichushi-fang)(here in after referred to SJB) on the obese gene and obese inhibitory, on C57BL/6 mice were induced by high fat diet. Methods : C57BL/6 mice were divided into three groups(normal, high fat diet with control, high fat diet with SJB extract) and fed for 15 weeks. And observed that, body weight change, final increase of body weight, the weight change of the adipocytes, the level change of ALT, AST, creatinine, total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, NEFA, glucose, the expression of ${\beta}3AR$, leptin, and $TNF-{\alpha}$ gene in primary adipocytes and adipocytes tissue. Results : The following results were obtained in this study. 1. SJB 500 mg/kg extract group showed considerable decrease in weight, the final increase of weight and the amount of adipocyte in weight. 2. All experimental group showed that the amount of ALT, AST, total cholesterol, LDL-cholesterol, triglyceride and NEFA were decreased considerably. SJB 500 mg/kg extract group showed the amount of HDL-cholesterol and leptin were increased considerably. 3. All experimental group showed that the revelation of ${\beta}3AR$ in primary adipose cell and 3T3-L1 cell were increased considerably, and that the revelation of leptin in primary adipose cell and 3T3-L1 cell were decreased considerably. SJB $100{\mu}{\ell}/m{\ell}$ extract group showed that the revelation of $TNF-{\alpha}$ were decreased considerably. 4. SJB 500 mg/kg extract group showed that the size of adipocyte in adipocytes tissue were decreased. 5. All experimental group showed that the adipose vacuoles in liver tissue were decreased considerably. Conclusions : Comparison of the results for this study showed that SJB is effective on obesity care and has obese-inhibitory effects in obese mouse induced by high fat diet. So it is respected that the clinical application of SJB can help the treatment of obesity.

Hypocholesterolemic Effect of Ginkgo Biloba Seeds Extract from High Fat Diet Mice

  • Kang, Hyun
    • 대한의생명과학회지
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    • 제23권2호
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    • pp.138-143
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    • 2017
  • To investigate the efficacy of extract of Ginkgo Biloba seeds in high fat diet (HFD) in mice, the Ginkgo Biloba seeds extract (GSE) was orally administered to mice with a HFD at 300 mg/kg/day for 4 weeks. Our results show that GSE significantly inhibited fat accumulation. Moreover, GSE markedly reduced the final body weight with a decrease in epididymal adipose tissue mass and adipocyte size compared with the untreated HFD-induced group. Additionally, GSE ameliorated serum high-density lipoprotein cholesterol levels. The results show that Ginkgo Biloba seeds possesses hypocholesterolemic effect through down regulating lipid metabolism. Further studies are required in this area to strengthen the anti-obesity effects of GSE with active component, and it can be used a pro-drug instead of whole extract.

Hypocholesterolemic Effect of Tartary Buckwheat (F. tataricum Gaertn.) Extract from High Fat Diet Mice

  • Lee, Sung-Gyu;Lee, Dongsup;Kang, Hyun
    • 대한의생명과학회지
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    • 제23권1호
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    • pp.34-38
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    • 2017
  • To investigate the efficacy of extract of Tartary buckwheat (F. tataricum Gaertn.) in high fat diet (HFD) in mice, the F. tataricum Gaertn. extract (FTE) was orally administered to mice with a HFD at 300 mg/kg/day for 4 weeks. Our results show that FTE significantly inhibited fat accumulation. Moreover, FTE markedly reduced the final body weight with a decrease in epididymal adipose tissue mass and adipocyte size compared with the untreated HFD-induced group. Additionally, FTE ameliorated serum total cholesterol, triglyceride, and low-density lipoprotein cholesterol levels. The results show that Tartary buckwheat possesses hypocholesterolemic effect through downregulating lipid metabolism. Further studies are required in this area to strengthen the anti-obesity effects of FTE with active component, and it can be used a pro-drug instead of whole extract.

Biocellulose Reduces Body Weight Gain of Rats Fed High-Fat Diet

  • Han, Dae-Seok;Kwon, Eun-Kyung;Kim, Dong-Woo;Kim, Young-Eon;Lee, Chang-Ho;Kim, In-Ho
    • Food Science and Biotechnology
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    • 제15권1호
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    • pp.70-76
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    • 2006
  • Anti-obesity effect of biocellulose and diet formulation containing it was evaluated using obese rats. Thirty male Sprague-Dawlely (SD) rats were randomly assigned to high-fat diet group (CON), high-fat diet group containing 5% biocellulose (BIO), and high-fat diet group containing 5% dietary formulation (DF). After 10 weeks, body weight gains of BIO and DF groups were reduced by 15.1 and 6%, respectively, as compared with CON group. Epididymus, parirenal, and visceral fat pads of BIO and DF groups were lower than those of CON group. Weight of interscapular brown adipose tissues increased slightly in BIO group and significantly in DF group. Size of adipocyte in rats decreased in BIO and DF groups. Results indicate biocellulose-containing diet has anti-obesity effect by reducing body weight gain and white adipocytes depots in rats fed high-fat diet.

C57BL/6 마우스에서 고지방 식이와 CCl4로 유발한 간섬유증에 미치는 커큐민의 보호효과 (Protective Effects of Curcumin on CCl4-Induced Hepatic Fibrosis with High Fat Diet in C57BL/6 Mice)

  • 제갈승주;민병운;박호
    • 대한임상검사과학회지
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    • 제47권4호
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    • pp.251-258
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    • 2015
  • 커큐민(curcumin)은 강황 내 주요 폴리페놀 화합물의 한 성분으로 비만과 관련된 비알코올성 지방간염을 막아 주는 것으로 잘 알려져 있다. 이 연구의 목적은 고지방 식이로 키운 비만 마우스에서 사염화탄소($CCl_4$)로 유발한 간섬유증에 미치는 커큐민의 보호효과를 조사하기 위해 시도하였다. 군간 비교를 위해, 정상 식이와 고지방 식이로 키운 마우스에 7주간 $CCl_4$를 투여하면서 동시에 커큐민을 투여한 것과 투여하지 않은 군으로 나누었으며, 체중이나 혈당과 같은 대사 프로파일이나 지방세포의 크기 및 간섬유증의 변화를 조사하기 위해 혈청 생화학적 검사, 조직학적 검사 및 면역조직화학적 검사를 수행하였다. 또한 간세포 내자멸세포의 관찰을 위해 TUNEL 법을 사용하였다. 그 결과 고지방식이+$CCl_4$ 마우스에 커큐민을 투여한 군에서는 투여하지 않은 군에 비해 체중, 공복혈당 수치, 혈청 AST, ALT 수치가 낮았고, 지방조직 내의 지방세포 크기와 대식세포 및 비만세포 수가 감소하였다. 이와 반대로 정상식이+$CCl_4$ 마우스에 커큐민을 투여한 경우에는 투여하지 않은 군에 비해 체중과 비만세포 수 이외에는 통계학적인 차이가 없었다. 더욱이 커큐민은 간조직의 간실질세포의 자멸세포 수는 줄인 반면 활성 간성상세포 모양의 비실질세포의 자멸세포 수를 증가시켰다. 이 결과를 종합해 볼 때 커큐민은 비만 마우스의 간질환 진행에서 효과적인 항섬유 치료제로서 사용할 수 있을 것으로 사료된다.

n-6/n-3 지방산 비율이 비만 랫드의 지질대사에 미치는 영향 (Effect of n-6/n-3 fatty acid ratio on lipid metabolism in obesity model rats)

  • 신종서;엄경환;박병성
    • 한국응용과학기술학회지
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    • 제35권3호
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    • pp.654-666
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    • 2018
  • 본 연구는 비만 모델동물 랫드에서 식이 내 오메가 6와 3 지방산 비율(n-6/n-3 0, 4:1, 15:1, 30:1)이 지질대사의 생화학적 메카니즘을 구명하였다. 혈액 중성지방은 대조군과 비교할 때 n-6/n-3 비율 4:1, 15;1, 30:1 처리군에서 22.21%, 총콜레스테롤 20.60%, LDL-C 52.96%, 혈당 15.71%, ALT 11.97%, AST 9.13%, 인슐린 37.57%, 렙틴 45.98% 낮아졌으나, HDL-C 28.38%, 인지질 80.39% 증가하였고, 특히 4:1 처리군에서 가장 큰 효과가 나타났다. 간 조직에서 SREPB-$1{\alpha}$, SREPB-2 mRNA는 대조군과 비교할 때 n-6/n-3 비율 처리군에서 하향조절 하였으나 PPARs 그리고 지방조직의 LPL-mRNA는 상향조절 하였다. 간 조직에서 지방세포의 크기는 n-6/n-3 비율 30:1, 대조군, 15:1, 4:1 순서로 낮아졌고, 지방조직에서 지방세포의 크기는 대조군, 30:1, 15:1, 4:1 순서로 낮았다. 결과는 비만 모델동물 랫드에서 오메가 6와 오메가 3 지방산 비율, 특히 4:1을 함유하는 식이를 급여하면 혈액 유해 지질, 간 기능 효소, 렙틴, 인슐린, 간과 지방조직의 유전자 발현 조절을 통하여 건강을 유지할 수 있다는 새로운 사실을 발견하였다.

db/db 마우스에서 비파의 혈당 저하 효과 (Hypoglycemic Effect of Eriobotrya japonica(E. japonica) in db/db Mice)

  • 김은;김민숙;류동영;민오진;백흠영;김용재;김현아
    • 한국식품영양학회지
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    • 제22권2호
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    • pp.159-165
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    • 2009
  • E. japonica is a well-known medicinal plant in Japan. The leaves of E. japonica were reported to have a hypoglycemic action. However, seeds of E. japonica are discarded and not used. To elucidate for anti-diabetic effects of E. japonica, Type 2 diabetic mice were allocated to control group, E. japonica leaf, and seed extract group. Animals were fed a 2018S Teklad global 18% protein rodent diet. Animals were received daily oral injections of E. japonica leaf or seed extract at a dose of 200 mg/kg body weight for 6 weeks. Body weight, food intake and water intake, and total adipose tissue weight of animals were significantly reduced by feeding of E. japonica leaf extract. All E. japonica extract groups significantly decreased fasting blood glucose, glycosylated hemoglobin levels, size of adipocytes and serum adiponectins. However, they did not have a beneficial effect on the serum triglyceride and cholesterol in the diabetic animals. These results suggest that E. japonica seed and leaf extracts have a antidiabetic effect by controlling of blood glucose and decrease of size of adipocytes in db/db mice and seed extract is more effective in hypoglycemic action than leaf extract.

칼슘과 제니스테인 섭취가 고지방식이로 유도된 비만 모델 마우스의 체지방과 지질대사에 미치는 영향 (Effects of Calcium and Genistein on Body Fat and Lipid Metabolism in High Fat-induced Obese Mice)

  • 김미현;김설희;박현우;김완기;이연숙
    • Journal of Nutrition and Health
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    • 제39권8호
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    • pp.733-741
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    • 2006
  • The study was conducted to investigate the effects of dietary calcium and soy isoflavone on body fat and lipid metabolism in high fat-induced obesity. Four week old female C57/BL6J mice, known as a good model of diet-induced obesity, were fed low Ca and high fat diet for 6 weeks. After induced obesity, mice were divided into six groups according to diets varying calcium contents (0.1 or 1.5%) and genistein contents (0 or 500 or 1,000 ppm). Body weight, fat pad (perirenal fat and parameterial fat), adipocyte size, serum total lipid and total cholesterol were significantly decreased by both high Ca intake and genistein supplementation. However, the effect of genistein supplementation showed in low Ca-fed groups. Serum LDL-cholesterol and TG were significantly decreased by high Ca intake and genistein supplementation, respectively. In liver, lipogenic enzymes (fatty acid synthase and malic enzyme) activity and TG were significantly decreased by both high Ca intake and genistein supplementation. This inhibitory effect of genistein on lipogenic enzymes showed in low Ca-fed groups. But liver total cholesterol and total lipid were significantly decreased by high Ca intake and genistein supplementation, respectively. Fecal excretion of total lipid, total cholesterol and TG were significantly increased by high Ca intake, not by genistein supplementation. In conclusion, high calcium intake and genistein supplement may be beneficial for suppression of obesity through direct anti-adipogenesis by decreasing fat weight and size and indirect anti-lipo-genesis by inhibiting lipogenic enzymes activity and improving lipid profile.

Anti-Obesity Effects of a Mixture of Fermented Ginseng, Bifidobacterium longum BORI, and Lactobacillus paracasei CH88 in High-Fat Diet-Fed Mice

  • Kang, Dayoung;Li, Zhipeng;Ji, Geun Eog
    • Journal of Microbiology and Biotechnology
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    • 제28권5호
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    • pp.688-696
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    • 2018
  • Ginseng and probiotics have anti-obesity effects in mice fed a high-fat diet (HFD). Absorption of ginsenoside and colonization of probiotics occur in the intestine. In this study, a mixture of fermented ginseng and two probiotics, Bifidobacterium longum BORI and Lactobacillus paracasei CH88, was administered to HFD-fed mice for 9 weeks. The mixture significantly suppressed weight gain (p < 0.05, n = 8) and lipid deposition in the liver and adipose tissues as well as increased the mice's food intake. The adipocyte size of the adipose tissue was significantly decreased in the mixture-fed group, especially when 0.5% fermented ginseng and $5{\times}10^8/ml$ of the two probiotics were used (p < 0.05, n = 10). The expression of TNF-${\alpha}$ in adipose tissue was efficiently downregulated in the mixture-fed group (p < 0.05, n = 4). The supplement also improved the mice's fasting blood glucose levels (p < 0.05, n = 8) and total cholesterol feces excretion (p < 0.05, n = 8). The mixture of fermented ginseng and B. longum BORI and L. paracasei CH88 could have an anti-obesity effect and suppress lipid deposit in the liver and adipose tissues.