• 제목/요약/키워드: adhesive protein

검색결과 71건 처리시간 0.036초

회화 문화재의 보존처리에 쓰이는 풀 - 전분과 단백질의 함량에 따른 접착력과 보존성을 중심으로 (Adhesives Used in Conservation Treatment of Oriental Paintings -Comparison of Adhesiveness and Conservativeness in Relation to Starch and Protein Contents of the Adhesive Formula)

  • 전지연;박지선;김강성
    • 보존과학회지
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    • 제12권1호
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    • pp.83-91
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    • 2003
  • 회화 문화재의 표장 및 보존처리에 쓰이는 풀은 그 문화재의 수명을 좌우할 정도로 중요하기 때문에 옛날부터 풀의 제조법에 관한 연구가 있어 왔고, 이에 대한 기록이 남겨져 왔다. 기록들을 종합해 보면, 대부분 풀의 제조법에 있어 소맥의 단백질을 제거하는 것에 대한 연구가 행해졌음을 알 수 있다. 이렇게 공통적으로 보여지는 단백질 제거는 단백질이 풀에 있어서 유익하지 못한 요소를 갖고 있다는 것이었다. 실제로 단백질 함유량에 따른 굴의 접착력과 보존성의 변화에 대한 실험을 해 보았는데, 그 결과를 종합해 보면, 미생물의 영양원인 단백질의 함량이 적을수록 접착력과 보존성은 좋아지지만, 유연성은 떨어졌다. 그러나 단백질의 함량이 적으면 적을수록 룰의 보존성은 좋아지기 때문에 회화 문화재 보존처리에 쓰여야 하는 풀은 보존성이 있고, 최소한의 농도로 최대한의 접착력을 지닌 또, 가역성이 있는 소맥 전분풀이었다.

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Partial Purification of Mussel Adhesive Protein for Mytilus Edulis and Preparation of Mussel Protein Hydrolysates

  • Sun, Nam-Kyu;Song, Kyung-Bin
    • Preventive Nutrition and Food Science
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    • 제5권3호
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    • pp.148-152
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    • 2000
  • Mussel adhesive protein (MAP) was extracted from Korean Mytilus edulis and then partially purified using Sephacryl S-300 gel permeation chromatography and reversed-phase high performance liquid chromatography. As an indicator of adhesiveness, is 3,4-dihydroxyphenylalanine (DOPA) content was determined. Its DOPA/protein ratio of 0.19 was higher than those of other reports, indicating a good adhesive. The partially purified MAP was confirmed by acid-urea polyacrylamide gel electrophoresis using cetylpiridinium bromide as a cationic detergent. Sea mussel hydrolysates were prepared using three commercial proteases to provide value-added functional materials and their angiotensin converting enzyme (ACE) inhibitory activities were determined. Among hydrolysates of sea mussel, Protamex was the best and further purification would improved ACE inhibitory activity.

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혈장변성접착제를 사용한 내수합판의 특성 (Properties of Water Resistant Plywood made with Modified Serum Protein Adhesive)

  • 강석구;이화형
    • Journal of the Korean Wood Science and Technology
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    • 제39권1호
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    • pp.21-27
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    • 2011
  • This study was carried out to examine properties of water resistant plywood by using serum protein adhesive which is natural, environment-friendly and human-friendly. For the preparation of the serum protein adhesive, pig blood from slaughterhouse was centrifuged and serum was separated from corpuscles and concentrated to 30% by dry weight basis. This concentrated serum protein was modified with PF resin (50% NVC) with the ratio of 9 : 2.5. Plywood made by this modified serum protein gave 1.21 N/$mm^2$ of dry bonding strength, 0.80 N/$mm^2$ of wet boil bonding strength, 0% of cyclic delamination test value, and 0.025 ppm of HCHO emission, which met the excellent super $E_0$ grade and water resistant plywood.

혈장접착제를 이용한 생활목질폐잔재로부터 제조된 흑탄 보드의 성질 (Properties of Black Charcoal Board Manufactured from Domestic Wood Waste by Using Serum Protein Adhesive)

  • 서인수;이화형
    • 농업과학연구
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    • 제37권2호
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    • pp.267-270
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    • 2010
  • This study was carried out to manufacture black charcoal board from domestic wood waste by using serum protein adhesive which is natural, environment-friendly and human-friendly. For the preparation of the serum protein adhesive, pig blood from slaughterhouse was centrifuged and serum was separated from corpuscles and concentrated to 30% by dry weight basis. The particle size of charcoal from domestic wood waste for this study was #6-60. Hot pressing schedule was $170^{\circ}C$ and 40kgf/$cm^2$ (1 min)-10kgf/$cm^2$ (2.5 min)-40kgf/$cm^2$ (5 min). The black charcoal board made by the addition of 13% serum protein adhesive on dry weight basis gave 41.76kgf/$cm^2$ of bending strength, 8.12kgf/$cm^2$ of internal bonding strength, and excellent gas adsorption and workability.

소맥전분 풀의 이화학적 특성 연구 - 수침기간이 2년 이하인 풀을 중심으로- (Physicochemical Characteristics Study on Wheat Starch Adhesive - Based on Wheat Starch Adhesive fermenting period less than two years-)

  • 정용재;김민정;남서진;정선혜
    • 펄프종이기술
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    • 제44권2호
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    • pp.35-41
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    • 2012
  • In this study, wheat starch adhesive was investigated the shape and structure of starch, the difference in characteristics such as chemical composition according to the fermenting period of 2 years or less. The fermenting period of wheat starch adhesive is 1 month, 2months, 4 months,6 months, 1 year, 2years old. The wheat starch adhesives were investigated total sugar contents, protein contents, properties of gelatinization, pH, the bonding strength and also observed the surface of starch,. As a result, the longer the fermenting period, the increasing in total sugar contents and decreasing in protein contents. The particle shape and surface were similar regardless of the period. In addition, properties of gelatinization according to the fermenting period also could not see the difference. In pH of the adhesive, the longer the fermenting period, the near to neutral. The adhesive was high bonding strength in 4 months, but appeared a tendency to decrease from 6 months. The damage assessment through the UV degradation in regard to the papers applied the adhesive was accomplished. Color difference was no change except 1 month. The 4 months and 6 months' pH was each 5.0, 5.2. But it was near to neutral that the 12 months and 24 months' pH was each 5.7, 5.9.

Synthesis of Wood Adhesive Derived from the Milk Protein and the Blocked Isocyanate

  • Ha, Yuna;Lee, Sang-Min;Lee, Hyang-Yeol
    • 한국응용과학기술학회지
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    • 제30권3호
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    • pp.551-559
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    • 2013
  • To investigate the adhesion effect of sodium bisulfite content in making blocked isocyanate, wood adhesive PB-1, PB-2, PB-3 and PB-4 containing sodium bisulfite content of 15%, 22.5%, 30% and 37.5% were synthesized respectively. As a result, when the amount of sodium bisulfite increased in adhesives, the tensile strength was found to be proportionally increased. The final adhesive mixtures were manufactured using a two-components system which are prepared by mixing two separate protein and BI solutions due to the precipitate in the adhesives. As PVA was added to adhesives to increase tenacity, the plywood dehiscence phenomenon after cold pressing process was declined. By addition of PVA, the tensile strength was improved up to $6.5{\sim}7kgf/cm^2$ with BI/protein ratio from 1:6 up to 1:8. Phase separation between milk fat and aqueous layer was disappeared after addition of emulsifier, Tween 20. Additon of Tween 20 showed tensile strength up to $5{\sim}6.5 kgf/cm^2$ at NCO/protein ratio of 1:12 ~ 1:14 without phase separation.

ATR-FTIR Analysis of Adhesives Jointing Buddhist Scripture Scrolls in Medieval Korea(Goryeo Dynasty)

  • Oh, Joon Suk
    • 보존과학회지
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    • 제35권1호
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    • pp.11-18
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    • 2019
  • The adhesives joining Buddhist scripture scrolls from medieval Korea(Goryeo Dynasty, A.D. 918~1392) are different from wheat starch adhesive. The composition of the adhesive was analyzed using Attenuated Total Reflectance-Fourier Transform Infrared(ATR-FTIR) spectroscopy. In the adhesive used to join Buddhist scripture scrolls, peaks attributed to amide I and amide II of the protein and carbohydrate were detected in the ATR-FTIR spectra, and no carbonyl peak($1745cm^{-1}$) for oil was detected in the 2nd derivative ATR-FTIR spectra. The ATR-FTIR spectra almost coincided with those of defatted soybean powder adhesive. Hence, the adhesives joining Buddhist scripture scrolls were inferred to be soybean adhesive prepared from a defatted soybean cake.

원자현미경을 이용한 생체물질의 접착력 측정기술 개발 (Novel measuring technique for biological adhesion forces using AFM)

  • 김성주;문원규;전종협
    • 한국정밀공학회:학술대회논문집
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    • 한국정밀공학회 2005년도 춘계학술대회 논문집
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    • pp.641-644
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    • 2005
  • The study on the interaction forces of some biological materials is important to understanding biological phenomena and their application to practical purpose. This paper introduces a measuring technique for biological adhesive forces using the AFM(Atomic Force Microscope). Since no standardized thesis on adhesive forces exist, the adhesive forces is defined as adhesive forces against a hardened surface of biological materials. To grant the results are meaningful, which is based on the understanding the surface characteristics of biological materials using the AFM, a nominal value of average adhesive force per unit area should be measured. Therefore the modified AFM probe with small micro glass bead was proposed so that it can guarantee the required contact area for measuring the average adhesive forces. A pyrex glass substrate with circular patterns, which was fabricated by micromachining technique, is introduced in order to controll the contact area. The two types of mussel adhesive proteins, Celltak and recombinant-MGFP5, were tested by the proposed measuring method. The test results show that the adhesive force of the mussel adhesive proteins can be reliably measured by use of this method.

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Surface Interactions of Model Peptides for Mussel Adhesive Protein

  • Cho, Eun-Kyung;Lee, Yoon-Hee;Cho, Nam-Jun;Cha, Hyung-Joon
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2003년도 생물공학의 동향(XIII)
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    • pp.630-633
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    • 2003
  • The mussel adhesive protein Mefp-1 is a natural, strong and durable adhesive that is stable under corrosive, saline conditions. Mefp-1 is found in the marine mussel Mytilus edulis and it has a molecular weight of ca. 130,000. The primary structure is mainly composed of repeating decapetides: Ala-Lys-Pro -Ser-Tyr Hyp-Hyp-Thr-DOPA-Lys. To elucidate the mechanism by which Mefp-1 bonds to metal surfaces, we have used surface-enhanced Raman spectroscopy to study the interactions of peptides related to the Mefp-1 decapeptide repeat with gold surfaces. We have concluded that the tyrosine residue and the carboxyl terminus interact strongly with the gold surface, and that proline and hydroxyproline constrain the conformations of the peptides, thereby limiting the types of possible interactions of the functional groups with the gold surface.

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Soybean-based Green Adhesive for Environment-friendly Furniture Material

  • Jeon, Ji-Soo;Lee, Jeong-Hun;Kim, Su-Min
    • 한국가구학회지
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    • 제22권3호
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    • pp.174-182
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    • 2011
  • Over the last decade, Sick Building Syndrome has become a significant social issue in Korea and many methods have been considered to maintain comfortable indoor air quality. To reduce toxic substances emitted from wood composite products, the source control is an efficient method through the reduction of formaldehyde content by using natural material-based adhesives for composite wood products production. Among alternative materials, soybean protein is considered an appropriate natural material to replace formaldehyde-based resin and many efforts have been made to produce new products, such as soap, shampoo, ink, resin, adhesive and textile through changing the chemical or physical properties of soybean. To process soybeans into these useful products, the beans are dehulled and the oil is removed by crushing at very high pressure or by solvent extraction. For use soybean as an adhesive, it is processed at temperatures below $70^{\circ}C$ to preserve the alkaline solubility of the proteins. In addition, soybean-based adhesive is undergone treatment process to improve mechanical properties using urea, urease inhibitor N-(n-butyl) thiophosphoric triamide and sodium dodecyl sulfate. The modified soybean-based adhesive exhibited sufficient mechanical properties to use as an adhesive for composite wood products. This paper is a review article to discuss the possibilities of soybean-based adhesive for environment-friendly furniture materials.

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