• Journal of Genetic Medicine
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• 제17권1호
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• pp.34-38
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• 2020

The term dopa-responsive dystonia (DRD) is used to describe a group of neurometabolic disorders, which are characterized by dystonia, and are typically associated with diurnal fluctuations and respond to levodopa treatment. Autosomal dominant DRD (DYT5a, MIM# 128230) is caused by a heterozygous mutation in the GTP cyclohydrolase 1 (GCH1) gene (MIM# 600225). GCH1 encodes an enzyme, which is involved in the biosynthesis of tetrahydrobiopterin, an essential co-factor for tyrosine hydroxylase. Herein, we report the case of a 16-year-old girl who was diagnosed with DYT5a. She exhibited additional unusual clinical features, including intellectual disability, depression, multiple skeletal anomalies, and short stature, which are not commonly observed in patients with DYT5a. The patient harbored a heterozygous missense variant, c.539A>C, p.Gln180Pro, in the GCH1 gene, which was identified by targeted gene panel analysis using next-generation sequencing.

• 생명과학회지
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• 제19권5호
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• pp.561-565
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• 2009

인간조직인자는 혈액응고인자 factor VII 과 복합체를 형성하며 연속적인 혈액응고 연쇄반응을 촉매하는 효소 활성체이다. 복합체 형성에 필수적인 이 조직인자의 세포 외 부분이, 기존의 융합 단백질 및 히스티딘 말단이 없는 새로운 발현 벡터에 의해 대장균 내에서 과량 발현 되었다. 봉입체 형태로 발현된 재조합 인간조직인자는 DEAE-Sephacel 크로마토그라피 기술을 적용하여 분리, 정제 및 구조적 복원이 동시에 시도 되었다. 정제된 재조합 단백질은 SDS-PAGE 분석에서 순수한 형태로 나타났으며, 생물학적 활성도 또한 기존의 조직인자와 거의 동등함을 보였다. 본 연구의 발현 및 정제 시스템은 이전의 보고에서 보여진 방법들에 비해 단백질 분해효소를 사용하지 않아 추가적인 크로마토그라피 과정이 필요 없어 좀 더 효율적이기 때문에 기존의 발현 시스템에 대해 대체할 수 있는 매우 유용한 방법으로 제공된다.

• 한국정보시스템학회지:정보시스템연구
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• 제11권1호
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• pp.51-74
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• 2002

The purpose of this study is to empirically investigate critical success factors for effective implementation of web-based distance learning system. First of all, four critical success factors are theoretically derived from reviewing previous research. They are: (1) learner-related factor including the variables such as teaming ability, learning attitude, and attending motivation, (2) environmental factor including the variables of physical and mental support for learners, (3) instructional design factor represented by one variable, the degree of appropriateness of learning contents, and (4) the factor concerning the level of self-directed learning readiness embracing the variables such as curiosity for learning, openness towards challenge of learning and affection for learning. Subsequently, the relationships between these four critical success factors and the degree of learning satisfaction are empirically investigated. The data for empirical analysis of the research are collected from 1,020 respondents who have already passed the web-based distance learning courses which have been implemented in Information and Communication Officials Training Institute. Out of 1,020 responded questionnaires, 875 data were available for statistical analyses. The main results of this study are as follows. Firstly, the most important factor for successful implementation of the web-based distance learning system is shown to be the instructional design factor, and in the next place, the self-directed learning readiness factor, the environmental factor and the learner-related one in sequence. Secondly, additional analysis of the variables included in the instructional design factor shows that availability of practical information and knowledge is the most influencing variable, and next, interesting composition of contents, reasonable learning amount, optimal level of instruction, and understandable explanation are significantly important in the descending order. Lastly, among learning motivators, strong intention of acquiring business knowledges and skills is found to be the most important satisfier in the web-based distance learning. The theoretical contribution of this study is to derive a comprehensive model of critical success factors for implementing the web-based distance learning system. And, the practical implication of this study is to propose efficient and effective guidelines for developing and operating the web-based distance learning system in the various kinds of organizations.

• Journal of Microbiology
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• 제45권6호
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• pp.534-540
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• 2007

The Streptomyces coelicolor A3(2) genome contains six operons (rrnA to F) for ribosomal RNA synthesis. Transcription from rrnD occurs from four promoters (p1 to p4). We found that transcripts from the p1 and p3 promoters were most abundant in vivo in the early exponential phase. However, at later phases of exponential and stationary growth, transcripts from the p1 promoter decreased drastically, with the p3 and p4 transcripts constituting the major forms. Partially purified RNA polymerase supported transcription from the p3 and p4 promoters, whereas pure reconstituted RNA polymerase with core enzyme (E) and the major vegetative sigma factor ${\sigma}^{HrdB}$ ($E{\cdot}{\sigma}^{HrdB}$) did not. In order to assess any potential requirement for additional factor(s) that allow transcription from the p3 and p4 promoters, we fractionated a partially purified RNA polymerase preparation by denaturing gel filtration chromatography. We found that transcription from the p3 and p4 promoters required factor(s) of about 30-35 kDa in addition to RNAP holoenzyme ($E{\cdot}{\sigma}^{HrdB}$). Therefore, transcription from the p3 and p4 promoters, which contain a consensus -10 region but no -35 for ${\sigma}^{HrdB}$ recognition, are likely to be regulated by transcription factor(s) that modulate RNA polymerase holoenzyme activity in S. coelicolor.

• 대한간호학회지
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• 제19권3호
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• pp.273-284
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• 1989

The aim of this investigation was to delineate concepts contributing to the Exercise of Self-Care Agency (E.S.C.A.) Scale developed by Kearney & Fleischer (1979) and to test its construct validity and its predictors. The results are summarized as follows ; 1. By means of principal factor analysis and maximal likelihood factor analysis upon data generated from 280 undergraduate students, the factors of Concern about Health Knowledge, self-Concept, Information - Seeking Behavior, Decision -making and Responsibility, Self-esteem, and Passivity emerged. The total percent of variance explained by the 6 factors was 75.1% 2. To assess factor independence and instrument homogeneity, correlations among the 6 factors were computed. The correlations ranged from .24 to .46 indicating that the factors and the items were not too similar or redundant. 3. Test-retest reliability of the total scale is r＝.70. Cronbach's $\alpha$ coefficient for internal consistency of the total scale is .86 and that of the factors ranged from .750 to .661 (only factor 6 .497). 4. In additional analysis of the Exercise of Self-Care Agency Scale in relationship to Multiple Health Locus of Control, Family Environmental Scale, and Cornell Medical Index using stepwise multiple regression, the Internal Health Locus of Control Score predicted 21.8%(F＝53.34, p＝.0001), Family Environmental Score 8.3%(F＝22.59, P＝.0001), Modified Cornell Medical Index Score 5.4%(F＝15.74, P＝.0001) of the score of the E.S.C.A.

• Structural Engineering and Mechanics
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• 제4권2호
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• pp.139-148
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• 1996

Vector algorithms and the relative importance of the four basic modules (computation of element stiffness matrices, assembly of the global stiffness matrix, solution of the system of linear simultaneous equations, and calculation of stresses and strains) of a finite element computer program for inelastic analysis of reinforced concrete shells are presented. Performance of the vector program is compared with a scalar program. For a cooling tower problem, the speedup factor from the scalar to the vector program is 34 for the element stiffness matrices calculation, 25.3 for the assembly of global stiffness matrix, 27.5 for the equation solver, and 37.8 for stresses, strains and nodal forces computations on a Gray Y-MP. The overall speedup factor is 30.9. When the equation solver alone is vectorized, which is computationally the most intensive part of a finite element program, a speedup factor of only 1.9 is achieved. When the rest of the program is also vectorized, a large additional speedup factor of 15.9 is attained. Therefore, it is very important that all the modules in a nonlinear program are vectorized to gain the full potential of the supercomputers. The vector finite element computer program for inelastic analysis of RC shells with layered elements developed in the present study enabled us to perform mesh convergence studies. The vector program can be used for studying the ultimate behavior of RC shells and used as a design tool.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2005년도 춘계 학술발표회 논문집
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• pp.677-686
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• 2005

The allowable bearing capacity of a pile, the most important factor in stability estimation, is determined by applying safety factor to the ultimate load or yield load. There are several but contradictory methods available in current design codes to estimate the allowable bearing capacity and the safety factor. This paper analyzes load-settlement curves obtained from 19 static load tests measured from 11 sites. At all tests, the load is applied until apparent failure is observed. The validity of the ultimate and yield load estimation method and load caculated from the settlement criterion is investigated through comparison with the measured data. In addition, a new procedure to estimate allowable load and safety factor is proposed. Additional data from field static load tests, such as those incorporated in this study, are needed to more reliably apply the proposed method in design practice.

• Tribology and Lubricants
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• 제17권3호
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• pp.236-243
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• 2001

In this study, flow factors are evaluated in terms of kurtosis using random rough surface generated numerically. As h/$\sigma$become large ø$\sub$x/, ø$\sub$y/, ø$\sub$fp/, approach to 1 and ø$\sub$s/, ø$\sub$fs/ to 0 asymptotically regardless of kurtosis. ø$\sub$x/, ø$\sub$y/, ø$\sub$fp/ increase with increasing kurtosis in the mixed lubrication regime. ø$\sub$s/, ø$\sub$fs/ is associated with an additional flow transport due to the combined effect of sliding and roughness. As h/$\sigma$ decreases ø$\sub$s/, ø$\sub$fs/ increase up to a certain point, and then decrease toward zero. This behavior can be attributed to the increasing number of contacts in the mixed lubrication regime. ø$\sub$x/ in the presence of elastic deformation on the surface is larger than ø$\sub$x/ in the absence of it because local film thickness(h$\sub$T/) increases by elastic deformation.

• 한국병원경영학회지
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• 제15권4호
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• pp.125-142
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• 2010

Objective : To determine demographic, clinical, health care utilization factors predicting unplanned readmission(within 28 days) to the hospital. Methods : A case-control study was conducted from January to December 2009. Multiple logistic regression was used to examine risk factors for readmission. 180 patients who had been readmitted within 28 days and 1,784 controls were recruited from an university hospital in Seoul. Results : Six risk factors associated with readmission risk were identified and include mail sex, medical service rather than surgical service, number of comorbid diseases, type of patient's room, lenth of stay, number of admissions in the prior 12 months. Conclusions : One of the association with readmission risk identified was the number of hospital admissions in the previous year. This factor may be the only risk factor necessary for assessing prior risk and has the additional advantage of being easily accessible from computerized medical records without requiring other medical record review. This risk factor may be useful in identifying a group at high readmission risk, which could be targeted in intervention studies. Multiple risk factors intervention approach should be considered in designing future prevention strategies.

• 터널과지하공간
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• 제30권1호
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• pp.63-75
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• 2020

터널 발파의 최외곽에서 발생할 수 있는 과대 여굴은 작업자 및 장비의 안전에 저해되며 숏크리트 등 지보량의 증가로 공사 비용이 증가시키는 주요 원인이다. 이러한 여굴은 화약 에너지와 암반의 특성 간의 복잡한 발생 메커니즘으로 인해 완벽한 제어가 매우 어렵다. 본 연구는 여굴 발생의 중요한 원인인 암반의 공학적 특성 중 무지보 막장 상태, 단축압축강도, 풍화도 및 불연속면의 특성(빈도, 상태 및 불연속면과 최외곽선과의 각도) 등과 발생한 여굴의 깊이와의 관계를 feed-forward 인공신경망을 통해 분석하였다. 이를 통해 통해 얻어진 각 인자의 가중치를 기초로 여굴저항도(Overbreak Resistance Factor: ORF)를 개발하였다. 더불어 여굴저항도를 이용한 터널 발파 여굴 관리 시스템을 제안한다.