• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.6
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• pp.1439-1443
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• 2008

The objective of this study was to evaluate the acute toxicity of Taeumjowi-tang in rats. SPF Sprague-Dawley male and female rats were administered orally with Taeumjowi-tang extract of 2,500 mg/kg(low dosage group), and 5,000 mg/kg(high dosage group). We daily examined number of deaths, clinical signs, body weights and gross findings for 14 days. No dead animal and no significant changes of body weights were found during the experimental period. In addition, no differences were found between control and treated groups in clinical signs, hematology, serum biochemistry, and other findings. In conclusion, Taeumjowi-tang extract did not show any toxic effects, and oral LD50 value was over 5,000 mg/kg in SD rats.

• Journal of Preventive Medicine and Public Health
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• v.43 no.2
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• pp.131-137
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• 2010

Objectives: Bisphenol A diglycidyl ether (BADGE) is a liquid compound obtained by condensation of two molecules of epichlorohydrin with one molecule of bisphenol A. General and reproductive toxicity with BADGE has been reported higher than 1000 mg/kg/day. This study was performed to show the effects of acute exposure to BADGE below 1000 mg/kg/day on the testis in adult male rats. Methods: BADGE was administered by gastric lavage in a single dose of 500, 750, 1000, and 2000 mg/kg/day in 8-week old male SPF Sprague-Dawley rats. The right testis was processed for light microscopic analysis. The left testis was homogenized and spermatids were counted to determine the daily sperm production and daily abnormal sperm production. The sperm count, sperm motility, and incidence of abnormal sperm were estimated in the epididymis. In testicular sections, the seminiferous tubules were observed for qualitative changes. The progression of spermatogenesis was arbitrarily classified as full-matured, maturing, and immature. The specimen slide was observed at 3 points and 10 seminiferous tubules were evaluated at each point. Results: The male rats exposed to single oral dose of BADGE at 750, 1000, and 2000 mg/kg/day were significantly increased the number of immature and maturing sperm on the testis. There were no significant differences with respect to sperm head count, sperm motility, and sperm abnormality in the BADGE treatment groups. Conclusions: These results suggest that single oral exposure of BADGE 750 mg/kg/day can affect adult male testis development.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.4
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• pp.762-765
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• 2008

Ephedrae herba(Ma-huang) has been used to treat respiratory conditions for over 5,000 years. The early 1990s, the herbal ephedra and other products containing ephedrine began to be promoted as weight loss aids in United States. The objective of this study was to evaluate the acute toxicity of hebal ephedra in rats. SPF Sprague-Dawley male and female rats were administered orally with herbal ephedra extract of 2,500 mg/kg(low dosage group), and 5,000 mg/kg(high dosage group). We daily examined number of deaths, clinical signs, body weights and gross findings for 14 days. No dead animal and no significant changes of body weights were found during the experimental period. In addition, no differences were found between control and treated groups in clinical signs, hematology, serum biochemistry, and other findings. In conclusion, herbal ephedra extract did not show any toxic effects and oral LD50 value was over 5,000 mg/kg in SD rats.

• Journal of Food Hygiene and Safety
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• v.8 no.3
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• pp.163-169
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• 1993

The acute toxicity of lAS-Na(Linear Alkylbenzene Sulfonate-Na) and MES (ASME, Alpha-Sulfo fatty acid Methyl Ester), surfactans, was eyaluated in ICR mice for 14 days. Mice aging 6 weeks were administered orally with 0, 1,000, 1,320, 1,780, 2,280, 3,000 mg/kg of lASNa or 0, 1,000, 1,560, 2,450, 3,830, 6,000 mg/kg of MES in saline. The body weight of the treated animals was not significantly different from the controls. The main clinical signs of animals treated with lAS-Na or MES were diarrhea, decreased motor acthity and piloerection. The congestion in small intestine was only gross finding in dead animals treated with two sulfactants. In this study, $LD_{50}$ values of lAS-Na and MES were eyaluated 1,319 mg/kg in male and 1,402 mg/kg in female mice, 2,040 mg/kg in male and 2,548 mg in female mice, respectiyely.

• The Journal of Korean Medicine
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• v.30 no.6
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• pp.27-34
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• 2009

Objectives: To evaluate the acute toxic effects and approximate lethal dose of Cheonggan extracts (CGX) in SD rats and beagle dogs. Methods: Male and female rats were divided into 4 groups (Control, CGX 1250, CGX 2500, CGX 5000) respectively and male and female dogs were divided into two groups respectively (Control, CGX 5000) respectively. A single oral dose of CGX was treated to the rats and dogs. Mortality, signs of gross toxicity, and behavioral changes were observed over 14 days. All animals were observed every hour for 4 hours after administration and once a day thereafter for 14 days. Body weights were determined at $0_{th}$, $7_{th}$, and $14_{th}$ days. All surviving animals were sacrificed and necrotized. Major organs were inspected visually for gross findings. Results: No animals died in any of the groups during the experimental period (2 weeks), rats or dogs. Body weights of rats and dogs during the experiment continuously increased in all groups but there was no significant change. No abnormal clinical signs were observed for 2 weeks after a single administration of CGX in any dose group of CGX, rats or dogs. No abnormal findings in major organs were observed in any group of rats or dogs. Conclusion: CGX does not have acute toxic effects in rats or dogs. Therefore, an approximate lethal dose is assumed to exceed 5000 mg/kg in both rats and dogs.

• Toxicological Research
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• v.22 no.4
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• pp.453-458
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• 2006

This study was carried out to investigate the acute toxicity of surfactin C in mice. Surfactin C was administered orally at does of 0, 381, 610, 977, 1562 and 2500 mg/kg. Number of deaths, clinical signs, body weights, feed and water consumptions, and biochemical examinations were investigated for 14 days after single oral administration of surfactin C. $LD_{50}$ value was over 2500mg/kg in mice. In addition, no differences were found between control and treated groups in clinical signs, body weight gains, hematology, serum chemistry, feed and water consumptions. The results indicate that surfactin C did not show any toxic effects at 2500 mg/kg in mice.

• Korean Journal of Pharmacognosy
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• v.33 no.1 s.128
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• pp.5-12
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• 2002

This study was performed to evaluate hepatoprotective effect of Daewhang-whangryunhaedok-Tang(DWT) on liver injured rats induced by $CCl_4$ and the acute oral toxicity of it in mice. The activities of serum transaminase(ALT/AST), alkaline phosphatase(ALP) and lactic dehydrogenase (LDH), the levels of serum total cholesterol(TC) and triglyceride(TG), change of liver enlargement, and inhibitory activities of lipid peroxidation, catalase and glutathione-S-transferase(GST) in liver microsome were determined in hepatotoxic rats induced by $CCl_4$ DWT DWT was significantly reduced the serum ALT, AST, ALP, LDH, TC and TG levels. And, the increase of lipid peroxidation, decrease of catalase and GST activities in the liver microsome of $CCl_4$-intoxicated rat were significantly improved by the treatment of DWT. Male and female mice were administered maximum dosages of 5,000 mg/kg b.w. of DWT. After single oral administration of DWT to mice, we observed them daily for 2 weeks. DWT did not induce any toxic signs in the mortalities, clinical signs, body weight changes, and gross necropsy findings of mice. Based on these results, it is concluded that DWT may have the hepatoprotective effect on $CCl_4$ induced hepatotoxicity in rats. Also, DWT may have no side effect and its $LD_{50}$ value may be over 5,000 mg/kg b.w. in mice.

• Journal of Food Hygiene and Safety
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• v.8 no.2
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• pp.97-103
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• 1993

The acute toxicity of orally administered poly(sodium acrylic acid-acrylic acid), powerfully water-absorbent polymer, was el'aluated in Sprague-Dawley rats and feR mice. Rats and mice aging 6 weeks were gavaged with 0, 2.0, 3.0, 4.4, 6.7, or 10 g of poly(sodium acrylic acidacrylic acid)/kg in com oil. No animal died by treatment and any toxic symptom was not observed in the treated animals during 2 weeks. The body weight of the treated animals was not significantly different from the controls. The results of macroscopic examination on the organs of the treated animals revealed no abnormal findings. Therefore, it was concluded that poly(sodium acrylic acidacrylic acid) was practically non-toxic when it was orally administrated to rats and mice up to 10 g/kg, and its $LD_{50}$ was evaluated more than 10 g/kg in rats and mice.

• Korean Journal of Environmental Agriculture
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• v.3 no.1
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• pp.45-51
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• 1984

This study was performed to evaluate the aquatoxicity of 5 chemicals (butachlor, isoprothiolane, probenazole, carbofuran, and cartap) to carp (Cyprinus carpio), discuss the impact on the $96 hr-LC_{50}$ values of the chemicals with the exposure time. In butachlor, we also compared the acute toxicity values between two exposure system, the continuous flow system and static state system, and measured the dissolved oxygen concentrations in the two systems. The acute toxicity values (96 hr-LC50 values) of the 5 chemicals were 0.25 ppm in butachlor, 10.0 ppm in isoprothiolane, 6.2 ppm in probenazole, 1.40 ppm in carbofuran, and 0.64 ppm in cartap, respectively. We also found that the $LC_{50}$ values were downed with increase of the exposure time.

• Korean Journal of Agricultural Science
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• v.45 no.2
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• pp.248-257
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• 2018

Overexpression of AtCYP78A7, a gene encoding a cytochrome P450 protein, has been reported to improve tolerance to drought stress in genetically modified (GM) rice (Oryza sativa L.). The aim of this study was to evaluate the potential allergenicity and acute oral toxicity of the AtCYP78A7 protein expressed in GM rice. Bioinformatics analysis of the amino acid sequence of AtCYP78A7 did not identify any similarities with any known allergens or toxins. It showed that no known allergen had more than a 35% amino acid sequence homology with the AtCYP78A7 protein over an 80 amino acid window or more than 8 consecutive identical amino acids. The gene encoding the AtCYP78A7 protein was cloned in the pGEX-4T-1 vector and expressed in E. coli. Then, the AtCYP78A7 protein was purified and analyzed for acute oral toxicity. The AtCYP78A7 protein was fed at a dose of 2,000 mg/kg body weight in mice, and the changes in mortalities, clinical findings, and body weight were monitored for 14 days after the dosing. Necropsy was carried out on day 14. The protein did not cause any adverse effects when it was orally administered to mice at 2000 mg/kg body weight. These results indicate that the AtCYP78A7 protein expressed in GM rice would not be a potential allergen or toxin.