• Title/Summary/Keyword: activity spectrum

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A comparative study on the phytochemical and anti-oxidant activity differences in HongJam prepared with various silkworm varieties

  • Nguyen, Phuong;Kim, Seong-Wan;Jo, You-Young;Beteta, Sofia Pallais;Kang, Sang Kook;Kim, Soo Bae;Kim, NamSook;Kweon, Hae Yong;Kim, Kee-Young;Koh, Young Ho
    • International Journal of Industrial Entomology and Biomaterials
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    • v.41 no.2
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    • pp.19-27
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    • 2020
  • The aim of this study was to elucidate the difference in the amount of phytochemicals and anti-oxidant activities of the freeze-dried 5th instar 3rd day silkworm powder (FDSP) and 7 HongJams (HJs). Three primary colors of light of 8 silkworm powders revealed why each sample had unique color. The visual and absorption spectrum analyses of 80% methanol (MeOH) extracts of 8 silkworm powders revealed that white jade (WJ)-FDSP extracts showing strong forest-green colors had different absorption spectrum from 7 HJs. Among 7 HJs, the absorption spectrum of light green silkworm (LGS)-HJ extract was different from those of other HJ extracts. The amounts of total flavonoids and phenolic compounds of WJ-FDSP were 423.0±9.21% and 252.3±12.86%, respectively, more than those of WJ-HJ. Among 7 HJs, the amounts of total flavonoids and phenolic compounds in LGS-and red silkorm (RS)-HJ extracts were significantly more than those of WJ-HJ extract. Consistent with the amounts of phytochemicals, radical scavenging activity and reducing ability of WJ-FDSP extract were significantly higher that other HJ extracts. Among 7 silkworm powder extracts, LGS-HJ extract showed significantly higher radical scavenging activity and reducing ability than those of WJ-HJ extract. But, RS-HJ extract only showed significantly higher radical scavenging activity than that of WJ-HJ extract. Taken together, the amounts of phytochemicals in 8 silkworm powders contributed on their colors and anti-oxidant activities.

Inhibition of Spoilage and Pathogenic Bacteria by Lacticin JW3, a Bacteriocin Produced by Lactococcus lactis JW3 Isolated from Commercial Swiss Cheese Products

  • Jeong, Min-Yong;Baek, Hyeon-Dong
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.558-561
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    • 2000
  • Strain JW3 was isolated from commercial Swiss cheese products and identified as a bacteriocin producer. Lactococcus lactis JW3 showed a broad spectrum of activity against most of the non-pathogenic and pathogenic microorganisms tested by the modified deferred method. Lacticin JW3 also showed a relatively broad spectrum of activity against non-pathogenic and pathogenic microorganisms as assessed using the spot-on-lawn method. It demonstrated a typical bactericidal mode of inhibition against Leuconostoc mesenteroides KCCM 11324.

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STANDARIZING THE EXTRATERRESTRIAL SOLAR IRRADIANCE SPECTRUM FOR CAL/VAL OF GEOSTATIONARY OCEAN COLOR IMAGER (GOCI)

  • Shanmugam, Palanisamy;Ahn, Yu-Hwan
    • Proceedings of the KSRS Conference
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    • v.1
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    • pp.86-89
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    • 2006
  • Ocean color remote sensing community currently uses the different solar irradiance spectra covering the visible and near-infrared in the calibration/validation and deriving products of ocean color instruments. These spectra derived from single and / or multiple measurements sets or models have significant discrepancies, primarily due to variation of the solar activity and uncertainties in the measurements from various instruments and their different calibration standards. Thus, it is prudent to examine model-to-model differences and select a standard reference spectrum that can be adopted in the future calibration and validation processes, particularly of the first Geostationary Ocean Color Imager (GOCI) onboard its Communication Ocean and Meterological Satellite (COMS) planned to be launched in 2008. From an exhaustive survey that reveals a variety of solar spectra in the literature, only eight spectra are considered here seeing as reference in many remote sensing applications. Several criteria are designed to define the reference spectrum: i.e., minimum spectral range of 350-1200nm, based completely or mostly on direct measurements, possible update of data and less errors. A careful analysis of these spectra reveals that the Thuillier 2004 spectrum seems to be very identical compared to other spectra, primarily because it represents very high spectral resolution and the current state of the art in solar irradiance spectra of exceptionally low uncertainty ${\sim}0.1%.$ This study also suggests use of the Gueymard 2004 spectrum as an alternative for applications of multispectral/multipurpose satellite sensors covering the terrestrial regions of interest, where it provides spectral converge beyond 2400nm of the Thuillier 2004 spectrum. Since the solar-activity induced spectral variation is about less than 0.1% and a large portion of this variability occurs particularly in the ultraviolet portion of the electromagnetic spectrum that is the region of less interest for the ocean color community, we disregard considering this variability in the analysis of solar irradiance spectra, although determine the solar constant 1366.1 $Wm^{-2}$ to be proposed for an improved approximation of the extraterrestrial solar spectrum in the visible and NIR region.

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NMR peak assignment for the elucidation of the solution structure of T4 Endonuclease V

  • Im, Hoo-Kang;Hyungmi Lihm;Yu, Jun-Suk;Lee, Bong-Jin
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1996.04a
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    • pp.183-183
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    • 1996
  • Bacteriophage T4 endonuclease V initiates the repair of ultraviolet (UV)-induced pyrimidine dimer photoproducts in duplex DNA. The mechanism of DNA strand cleavage involves four sequential stens: linear diffusion along dsDNA, pyrimidine dimer-specific binding,l pyrimidine dimer-DNA glycosylase activity, and Af lyase activity. Although crystal structure is known for this enzyme, solution structure has not been yet known. In order to elucidate the solution structure of this enzyme NMR spectroscopy was used. As a basis for the NMR peak assignment of the protein, HSQC spectrum was obtained on the uniformly $\^$15/N-labeled T4 endonuclease V. Each amide peak of the spectrum were classified according to amino acid spin systems by interpreting the spectrum of $\^$15/N amino acid-specific labeled T4 endonuclease V. The assignment was mainly obtained from three-dimensional NMR spectra such as 3D NOESY-HMQC, 3D TOCSY-HMQC. These experiments were carried out will uniformly $\^$15/N-labeled sample. In order to assign tile resonance of backbon atom, triple-resonance theree-dimensional NMR experiments were also performed using double labeled($\^$15/N$\^$13/C) sample. 3D HNCA, HN(CO)CA, HNCO, HN(CA)HA spectra were recorded for this purpose. The results of assignments were used to interpret the interaction of this enzyme with DNA. HSQC spectrum was obtained for T4 endonuclease V with specific $\^$15/N-labeled amino acids that have been known for important residue in catalysis. By comparing the spectrum of enzyme*DNA complex with that of the enzyme, we could confirm the important role of some residues of Thr, Arg, Tyr in activity. The results of assignments were also used to predict the secondary structure by chemical shift index (CSI).

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The Effect of Project Spectrum Linguistic Activities on Young Children's Creativity (프로젝트 스펙트럼의 언어활동이 유아의 창의성에 미치는 효과)

  • Park, Joo-Sung;Kim, Cheon-Hee;Park, Yun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.7
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    • pp.1685-1695
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    • 2009
  • The purpose of this study was to examine the effect of linguistic activity on young children's Creativity by applying an linguistic activity, one of the major realm of Project Spectrum, to experimental group. The following research task was established for this study. What effect does the linguistic activity through Project Spectrum on children's Creativity? The participants of this study were 80 of 5-year-old young children in two groups. They were divided and assigned into two groups, an experimental group(26 boys and 14 girls) and a control group(22 boys and 18 girls). While the experimental group was engaged in the Project Spectrums linguistic activities for 15 weeks, the control group was engaged in kindergarten curriculum linguistic activities. It was revealed that Creativity through Project Spectrum of experimental group had much influence on children's linguistic ability than control group in every field (speaking, listening, reading, and writing) by developing and applying detailed criteria for Creativity for this study.

Studies on antibiotic against wheat black rust (II) (밀의 항흑수병 항생물질의 연구 II)

  • 정영기
    • Korean Journal of Microbiology
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    • v.19 no.3
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    • pp.115-120
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    • 1981
  • The substance produced by Bacillus subtilis $480HS_{20}$ showed specific anti Puccinia graminis activity and narrow antimicrobial activity. The anti Puccinia graminis substance was found to be peptide antibiotic which had molecular weight of about 1,500 by sephadex LH-60 gel-filtration. The amino acid composition of the substance waas composed of Ser (1), Glu(3), Ile(1), Gyr(2) and His (1). The compound was yellowish powder and it's melting point was $214{\sim}216^{\circ}C$. UV spectrum in methanol showed single peak at 278(E 1% 1cm 15.2) and important peak at 3,350-3,320, 2,940, 1,635, 1,520-155, 1,240-1, $230cm^{-1}$ were observed in IR Spectrum. The substance was soluble in methanol but insoluble in water, ethyl acetate and chloroform. The antibiotic $480HS_{20}$ showed a little antimicrobial activity against Pyricularia aryzae but no antimicrobial activity was observed against Gram + and Gram - bacteria and molds except pyricularia oryzae.

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Synthetic $\beta$-Lactam Antibiotics IV. Antibacterial activity of some 7 $\beta$-[2-(2-Aminothizol-4-yl)-2-(methoxyimino) acetaido]-3-(1-alkyl-1H-terazol-5-yl)thiomethyl-cephalospoins

  • Lee, Duck-Hyung;Kim, Sam-Min;Park, Sang-Woo;Kim, You-Seung
    • Archives of Pharmacal Research
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    • v.13 no.4
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    • pp.385-387
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    • 1990
  • 3-[(1-Methyl-1H-tetrazol-5-y)thiomethyl] is one of the most important side chains in cephaloporins. This side chain which occures in numerous antibacterial agents, suc as cefamandole, latamoxef, cefoperazone, and cefmenoxime, contributes remarkable potency and broad spectrum. For the continuous work of study on the development of broad spectrum cephalosporins, we become interested in the effect of structural modification of the substituent at the N-1 position of mercaptoterazole toward biological activity because our recent work had demonstraated that the arylsubstituted mercaptotetrazoles (Fig. 1) had favorable effect on the activity against gram-positive bacteria. The main focus of this report was to investigate the relationship between activity and functional groups in the mercaptotetrazole.

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Spectrum Analysis of Surface Discharge for Polymer Insulator According to Various Contamination (여러 가지 오손에 따른 고분자애자의 표면방전 스펙트럼 분석)

  • Park, Jae-Jun
    • The Journal of Information Technology
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    • v.8 no.1
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    • pp.113-118
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    • 2005
  • Investigation of surface discharge characteristics of surface leakage on polluted EPDM insulator have been performed. This work was performed utilizing High Frequency Current Trans former to monitor surface discharge. It was found that there were significant variation in the surface discharge waveform frequency spectrum, depending both on the surface discharge magnitude and more importantly on the duration of surface discharge activity.

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NEW DEVELOPMENT OF HYPERGAM AND ITS TEST OF PERFORMANCE FOR γ-RAY SPECTRUM ANALYSIS

  • Park, B.G.;Choi, H.D.;Park, C.S.
    • Nuclear Engineering and Technology
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    • v.44 no.7
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    • pp.781-790
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    • 2012
  • The HyperGam program was developed for the analysis of complex HPGe ${\gamma}$-ray spectra. The previous version of HyperGam was mainly limited to the analysis of ${\gamma}$-ray peaks and the manual logging of the result. In this study, it is specifically developed into a tool for the isotopic analysis of spectra. The newly developed features include nuclide identification and activity determination. An algorithm for nuclide identification was developed to identify the peaks in the spectrum by considering the yield, efficiency, energy and peak area for the ${\gamma}$-ray lines emitted from the radionuclide. The detailed performance of nuclide identification and activity determination was accessed using the IAEA 2002 set of test spectra. By analyzing the test spectra, the numbers of radionuclides identified truly (true hit), falsely (false hit) or missed (misses) were counted and compared with the results from the IAEA 2002 tests. The determined activities of the radionuclides were also compared for four test spectra of several samples. The result of the performance test is promising in comparison with those of the well-known software packages for ${\gamma}$-ray spectrum analysis.

Structural Analysis of Major Antimicrobial Substance Obtained from Leaf Mustard(Brassica juncea) (갓(Brassica juncea)의 주 항균물질의 구조 분석)

  • 강성구
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.5
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    • pp.702-706
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    • 1995
  • A major component(compound A) in the ethylacetate fraction exhibited a strong antimicrobial activity was identified by UV, IR, FABMS and NMR. The compound A showed strong absorbance at 209, 259 and 359nm, indicating a flavonoid ring structure. IR spectrum possessed absorbance of OH at 3400∼3300cm-1, ketone at around 1650cm-1, and aromatic C=C at around 1660cm-1. Molecular weight of the compound A calculated as 478 from the information of m/z 479(M+H)+ and m/z 477(M-H)+ in the FABMS spectrum. Molecular formula of this compound was found to be C22H22O12 from m/z 479.1220(+3.1mmu for C22H23O12) of HRFABMS spectrum and from 13C-NMR spectrum. 1H-NMR and 13C-NMR spectra of the compound A revealed aromatic proton and benzene rings. Distortionless enhancement by polarization transfer(DEPT) exhibited that the compound A possessed 10 quaternary carbons and 3 substituted benzene rings including a methoxy group substitution. The compound A was identified as isorhamnetin 3-O-β-glucopyranoside by spectrophotometric methods in conjunction with 1H-1H COSY, 1H-13C COSY and HMBC, which revealed a flavone with OH group at 3, 5, 7, and 4' and methoxy group at 3' positions esterified to glucose.

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