• 한국응용곤충학회지
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• 제21권1호
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• pp.23-26
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• 1982

Cercospora kikuchii에 의해서 발병된 대두종자의 부패균에 대한 획득저항성기작을 구명코자 본 연구를 실시하였다. 1. 대두의 건전종자, 자연열개종자, 인위적 상처종자 및 자반병 감염종자재의 전단백질 함량에는 차이가 없었으나 peroxidase활성도는 감염종자에서 월등히 높았다. 2. 대두의 건전종자와 자반병 감염종자의 종피 및 자엽내의 단백질함량에는 큰 차이가 없었으며 peroxidase활성도는 자엽에서는 차이 가 없었으나 종피에서 건전한 종피보다 자반병에 감염된 종자가 2.5배 정도 높은 활성도를 보였다. 3. Polyphenol oxidase는 대두의 건전종자의 자반병 감염종자 모두 활성도가 너무 낮아 측정할 수 없었다. 4. 전기영동에 의한 peroxidase isozyme pattern은 건전종자와 감염종자간에 차이가 없었다. 5. 자반병에 대한 저항성기작은 자반병균에 의하여 대주종피 자체내에서 생화학적 방어기작이 더욱 활성화 하였으며 peroxidase활성도의 증가는 병원균에 대한 반응으로 사료된다.

• Journal of Plant Biology
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• 제38권1호
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• pp.55-62
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• 1995

벼(Oryza sativa L. cv. Kye Hwa) 성숙종자에서 유도한 배발생 세포(embryogenic cell, EC)의 동위효소 발현양상과 효소활성을 조사하였다. 배발생 캘러스로부터 확립된 EC 현탁배양은 세포가 둥글며 세포질이 충만한 세포들로 이루어졌으나 비배발생 세포(nonembryogenic cell, NEC)의 현탁배양은 크게 신장한 액포화된 세포들로 구성되었다. 이러한 EC와 NEC의 peroxidase, esterase, acid phosphatase 그리고 malate dehydrogenase의 동위효소 양상과 활성도를 분석한 결과 밴드의 수와 특이성 그리고 밴드 활성 등에 현저한 차이를 보여주었으며 또한 이들 동위효소의 효소 활성도 측정 결과 EC에서 훨씬 더 높게 나타났다. NEC에는 나타나지 않은 EC의 특이 밴드와 강한 밴드 활성 그리고 높은 효소 활성 등은 EC의 형태적, 분화능과의 관련성을 내포하고 있다. 이러한 결과들은 이들 효소의 특이 밴드들이 벼에서 EC의 생화학적 표지자로서 사용될 수 있음을 내포하고 있다.

• Journal of Plant Biotechnology
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• 제49권3호
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• pp.250-256
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• 2022

This study was conducted to measure changes in polyphenol components and antioxidant effects of Cirsium Lineare (Thunb.) after fermentation by lactic acid bacteria. First, Cirsium Lineare (Thunb.) extract (CE, unfermented) and Cirsium Lineare (Thunb.) extract fermented with Lactobacillus paracasei (FCE) were prepared. Changes in components resulting from fermentation were confirmed through changes in polyphenol compound content and silymarin derivative pattern, and antioxidant activity was confirmed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, and ferric reducing antioxidant power (FRAP) analyses. As a result, polyphenol contents of CE and FCE were confirmed as 21.94 ± 1.15 and 67.90 ± 4.48 mg GAE/g, respectively. Both values were increased approximately three times by fermentation, and there was also a change in the silymarin derivative pattern. In the case of DPPH radical RC₅₀ values in particular, CE and FCE were confirmed to inhibit DPPH radicals by 50% at concentrations of 129.44 ± 5.85 and 50.00 ± 3.47 ㎍/mL, respectively, with the FCE value approximately 2.5 times lower than that of CE. In addition, ABTS radical scavenging and FRAP activity were confirmed to share similar trends as DPPH radical scavenging activity. When CE and FCE were compared, FCE showed a better antioxidant effect overall. In conclusion, this study suggested that FCE prepared through lactic acid bacteria fermentation may be utilized as a powerful antioxidant material.

• 한국컴퓨터정보학회논문지
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• 제14권5호
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• pp.157-165
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• 2009

본 논문에서는 일상 공간에서 발생할 수 있는 인간의 일상생활 행위(ADL: Activities of Daily Living)들을 인지하는 분산 모델을 제시한다. 사용자의 환경, 위치 및 행위 정보를 간단한 센서들이 부착된 가정용 기기 혹은 식기들을 통해 무선 센서 네트워크로 수집하며 분석하고, 이 정보를 기반으로 사용자의 생환패턴, 건강상태 등을 파악하여 이에 요구되는 라이프케어 서비스를 제공한다. 하지만 서비스의 제공을 위해서는 높은 수준의 행위인지 데이터가 요구되나 충분히 분석되어지지 않은 센싱 데이터들은 고차원 상창 추론을 위한 일상생활 행위 인지 모델의 구축을 어렵게 한다. 그러나 수집 데이터의 순서를 통해 행위를 인지할 수 있다는 것에 착안하여 센서 데이터들의 순서를 특정 행위 패턴을 분석하는 데 활용하고, 이를 기반으로 한 분산 선형 시간추론 알고리즘을 제안한다. 이 알고리즘은 가정, 사무실 및 병원과 같은 소규모 환경에서 행위를 인지하는 데 적절하다. 제안한 알고리즘의 성능평가를 위해서 MIT Media Lab에서 제공하는 공개 데이터를 사용하였으며, 75% 이상의 평균 행위 인지 정확도를 보였다.

• 부모자녀건강학회지
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• 제3권2호
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• pp.25-40
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• 2000

The purpose of this study is to identify the sleep/activity patterns of neonates and to provide a baseline data in developing an effective maternal-child nursing intervention strategies. The subjects of this study were 57 normal newborn babies from birth to 4 weeks of age, who visited the Postpartum Care Center and two General Hospitals and One Local clinic in Taejon. The data were collected from October 28th, 1999 to April 28th, 2000. The instrument used in this study was NCASA developed by Barnard and validated by the Academic Society of Parent-Child Health. The data were analyzed with the SPSS PC+ 7.5 for window program using means, standard deviation, one-way ANOVA. The results of this study were as follows: 1. The mean amount of daytime sleep was 7.45 hours, nighttime sleep was 4.27 hours, and the total daily sleep was 11.72 hours. The mean amount of longest sleep period was 4.32 hours. The mean regularity of daytime sleep was 35.55%, the nighttime sleep was 44.82%, and the total daily sleep was 39.02%. The mean frequency of nighttime awakenings was 2.44 times. 2. The mean amount of daytime awake periods was 8.54 hours, the nighttime awake periods was 3.72 hours, and the daily total awake periods was 12.27 hours. The mean amount of the longest awake period was 3.98 hours. 3. The mean frequency of daytime feeding was 5.18 times, the nighttime feeding was 2.50 times, and the total daily feeding was 7.49. The mean of regularity of total daily feeding was 61.34%. 4. According to an analysis of sleep patterns based on newborn's age, there were some statistically significant differences noted in the following factors ; amount of nighttime sleep (F=7.65, p=.002), longest sleep period(F=5.84, P=.006). 5. According to an analysis of activity patterns based on newborn's age, there were some statistically significant differences noted in the following factors ; amount of nighttime activity (F=7.64, P=.002), longest activity period (F=4.34, P=.020), frequency of nighttime feeding(F=3.89, p=.029), frequency of total daily feeding(F=3.76, P=.033), and regularity of daily feeding(F=4.66, P=.016). In conclusion, the newborn baby slept more during the daytime than nighttime and more active during the daytime. And there were some irregular sleep/activity pattern noted during 1 to 2 weeks of newborn baby. The results of this study will contribute to maternal-child health nursing practice and nursing research, and provide an information to parents about what to expect their newborn baby.

• 생태와환경
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• 제47권1호
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• pp.24-31
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• 2014

본 연구는 온대활엽수림에서 초식곤충의 먹이 활동이 계절 및 고도에 따라 어떻게 변하는 가를 알아보기 위하여 수행되었다. 조사는 지난 3년간(2011~2013) 지리산국립공원의 고도가 다른 세 지역(피아골, 시암재, 노고단)에서 이루어졌다. 조사방법은 각 지역에 서식하는 수종(3종)을 선정하여 4월부터 6월까지 1~2주마다 초식곤충의 식흔 활동을 식흔지수를 이용하여 관찰하였다. 조사 결과 고도가 가장 낮은 피아골(${\approx}300m$)에서 가장 먼저 식흔이 나타났고, 중간고도인 시암재(${\approx}900m$)에서는 가장 많은 식흔이 조사되었다. 고도가 가장 높은 노고단(${\approx}1300m$)에서는 식흔이 가장 늦게 나타났지만 짧은 시간에 급속히 식흔의 총량이 증가하였다. 또한 기존에 발표된 연구를 토대로 한 결과 초식곤충의 활동시기는 조류(곤줄박이)의 첫 산란일과 거의 비슷한 것으로 나타나 식물-곤충-2차 소비자(조류)와 밀접한 관련을 보여준다. 기후 환경이 변함에 따라 초식곤충 활동의 기간이 바뀌게 될 것이며 이러한 먹이사슬의 변동을 감시할 수 있는 지속적인 장기 생태 모니터링이 필요하다.

• 대한의용생체공학회:의공학회지
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• 제16권2호
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• pp.201-208
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• 1995

The information obtained from the analysis of respiratory muscle elecromyographic (EMG) activities provides a mean for studying muscular activity in relation to the ventilatory process. Thus, in order to comprehend the airflow pattern and its brain control, signal processing is required to characterize respiratory muscle activity. This paper presents a computerized method for the analysis of the electrical activity of the respiratory muscles of premature lambs, and focuses upon the automatic determination of respiratory timing points such as onset and cessation points of the burst activity. Based on experimental results, reliable timing points can be obtained using the proposed methodology.

• BMB Reports
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• 제31권6호
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• pp.554-559
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• 1998

Activities of glutathione- and thioredoxin-related enzymes and phenylpropanoid-synthesizing enzymes were measured and compared in the developing leaves of Arabidopsis thaliana. Phenylalanine ammonia-lyase activity is maximal in the leaves of 2-wk-grown Arabidopsis. Tyrosine ammonia-lyase activity is maximal in the leaves of 3-wk-grown and 4-wk-grown Arabidopsis. Activity of thioitransferase, an enzyme involved in the reduction of various disulfide compounds, is higher in younger leaves than in older ones. A similar pattern was obtained in the activity of thioredoxin, a small protein known as a cofactor of ribonucleotide reductase and a regulator of photosynthesis. Activity of glutathione reductase is also higher in the younger leaves. Malate debydrogenase activity remains relatively constant during the development of Arabidopsis leaves. The results offer preliminary information for further approach to elucidate the mechanism of growth-dependent variations of these enzymes.

• BMB Reports
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• 제28권6호
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• pp.490-493
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• 1995

We investigated the effect of hydrogen peroxide-treated metallothionein on the hepatic xanthine oxidase activity in vitro. When the metallothionein was preincubated with 1 mM of hydrogen peroxide, the activity of xanthine oxidase and type conversion were elevated dose-dependently by the addition of metallothionein into the reaction mixture. While increasing the treatment of hydrogen peroxide to the $50{\mu}g$of metallothionein, the xanthine oxidase activity and type conversion ratio were remarkably elevated dose dependently compared to the control. When cadmium ion was added to the reaction mixture, the increasing pattern of the enzyme activity was similar to the effect of hydrogen peroxide-treated metallothionein. DTT or penicillamine restored the increasing activity and type conversion of xanthine oxidase by the cadmium ion to the control level.

• The Korean Journal of Physiology
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• 제29권1호
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• pp.103-111
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• 1995

Intracellular recordings were obtained from Purkinje cells in rat cerebellar slices maintained in vitro. Adding tetrodotoxin to the superfusion solution produced a typical pattern of repetitive burst firing consisting of a cluster of action potentials followed by a long hyperpolarization. TTX-induced oscillatory activity was not due to modulation of membrane potential although underlying mechanisms for maintenance of oscillatory activity were influenced by membrane voltage. The mechanism of TTX-induced oscillation was not related to the presence or amplitude of $I_h$ and could still induce the oscillatory activity after blockade of $I_h$ by cesium. The result from an experiment in which QX-314 was injected intracellularly strongly suggested that TTX-induced oscillatory firing activity was due to blockade of post-synaptic $Na^{+}$ currents intrinsic to PCs.