• 한국환경보건학회지
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• 제40권3호
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• pp.255-263
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• 2014

Objectives: This study evaluated the fungicidal efficacy of a fumigant containing 20% ortho-phenylphenol against Trichophytone mentagrophytes (T. mentagrophytes), Candida albicans (C. albicans) and Aspergillus niger (A. niger). Methods: Five replicates of each carrier were contaminated by depositing 0.05 mL of each fungal suspension. After drying, two carriers without exposure to the fumigant and three carriers with exposure to the fumigant were left in a sealed room ($25m^3$) at $21{\pm}0.5^{\circ}C$ and $60{\pm}10%$ relative humidity for 15 hours. Immediately after removal from the test room, each carrier was transferred into recovery diluent and suspended, diluted and inoculated. After incubation, the numbers of each colony were counted, and the parameter values (N, T, d) were calculated. Results: The working culture suspension number (N value) of T. mentagrophytes, C. albicans and A. niger were $1.0{\times}10^8$, $1.2{\times}10^8$ and $5.7{\times}10^7CFU/mL$, respectively. All the colony numbers on the carriers exposed to the fumigant (n1, n2, n3) were higher than 0.5N1 (the number of fungal test suspensions by pour plate method), 0.5N2 (the number of fungal test suspensions by filter membrane method) and 0.5N1, respectively. In addition, all mean numbers of test strains recovered on the control-carriers (T value) were over $10^6CFU/mL$. For the fungicidal effect of the fumigant, all numbers of fungal reductions after exposure of the fumigant (d value) were 4 logCFU/mL. Conclusions: The present study showed that fumigant containing 20% ortho-phenylphenol has effective fungicidal activity against T. mentagrophytes, C. albicans and A. niger.

• 한국도서관정보학회지
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• 제38권1호
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• pp.195-210
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• 2007

이 연구는 재중 조선인의 조선문 잡지 출간상황과 발전방안을 살핀 것이다. 재중 조선문 잡지는 중국 해방 후부터 현재까지 20여종이 출간되었다. 이들 잡지는 대체로 정치적인 영향이나 독자층을 고려해서 정간, 복간, 개명 등의 변화를 가져왔다. 또한, 주제로 구분해 보면 정치, 문학, 예술분야의 잡지가 주종을 이룬다. 한편 잡지발간의 어려움의 요인은 조선족 인구감소 인구이동. 중국 정부의 지원축소와 폐지, 재료비 인상 등을 들 수 있다. 이를 극복하기 위해서는 한국어 독자층을 확대하고 2개 연어(중국어-한국어)로 구성된 잡지를 발간하며, 중국 내외의 활발한 문화교류를 개척하며, 한국으로부터의 다양한 지원이 강구되어야 한다.

• Journal of Plant Biology
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• 제12권2호
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• pp.7-14
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• 1969

Dunaliella tertiolecta did not show any increase in respiration rate when supplied with glucose, glycerol, sucrose, L-alanine, acetate, pyruvate and succinate. This was in contrast to Chlorella pyrenoidosa, which, under identical conditions, showed significant increase when supplied with glucose or acetate but not with the other compounds. Production of 14CO2 from added 14C-glucose in D. tertiolecta was lower than the other 14C-labelled substrates: L-alinine, glycerol, succinate, but higher than 14C-sucrose addition. And it was also lower than C. pyrenoidosa experiments which was added 14C-glucose as a substrate. Light reduced amounts of labelled carbon dioxide from 14C-glucose or 14C-acetate and increased incorporation of 14C from the substrates to cell materials in either D. tertiolecta or C. pyrenoidosa. The contribution of 14C from 14C-glucose to 14CO2 in cell-free system of D. tertiolecta were much higher than in whole cell suspension. It was contrast to C. pyrenoidosa which were showed reduction of 14CO2 production in cell-free systems than whole cell suspensions. When cell-free systems of D. tertiolecta and C. pyrenoidosa were supplied with ATP, NAD, NADP or/and hexokinase, it was remarkably increased production of 14CO2 from the substrates than the control. It was concluded that the low ability of D. tertiolecta to metabolize glucose were caused by the impermeability of the cell membrane to glucose and were not due to deficiencies of enzyme systems concerning glucose metabolism. In the cell-free systems, it seemed to be more active pentose phosphate pathway than glycolytic pathway in D. tertiolecta.

• 한국철도학회논문집
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• 제17권4호
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• pp.233-244
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• 2014

이 연구에서는 자기부상열차-가이드웨이 상호작용 해석기법을 현장에서 계측된 자료를 활용하여 검증한다. 자기부상열차의 차체와 대차는 병진운동과 회전운동을 하는 강체 질량으로 모사한다. 대차의 부상전자석에서 발생하는 부상력은 능동 제어 알고리즘에 의해 제어된다. 가이드웨이는 Euler-Bernoulli beam을 사용하여 수치 모형을 구성한다. 대차의 회전 운동으로 인한 부상공극의 변화와 가이드웨이에 작용하는 위치의 변화를 엄밀히 고려하여 자기부상열차-가이드웨이 상호작용계의 동적거동 해석을 수행한다. 인천공항 자기부상철도 노선에서 계측된 실측 자료를 이용하여 해석기법을 검증한다. 해석 결과와 현장 계측 결과의 비교를 통해 해석 기법의 정확성을 검증하고, 대상 구조물이 설계 기준을 만족하도록 안전하게 설계되고 시공되었음을 확인한다.

• The Korean Journal of Physiology
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• 제17권2호
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• pp.125-133
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• 1983

Red cell glycolytic intermediates, metabolites and metabolic ratios were studied. Glycolytic intermediates were measured in neutralized perchloric acid extracts of red cell suspensions after 3 hr incubation at $37^{\circ}C$ in the presence and absence of saponin. Adenosine triphosphate(ATP), adenosine diphosphate(ADP), pyruvate and lactate were measured by enzymatic procedures involving stoichiometric oxidation or reduction of a pyridine nucleotide. Glucose was determined using glucose oxidase after zinc hydroxide extraction. The redox state was calculated from the lactate dehydrogenase equilibrium. Adenosine triphosphatase activity(ATPase) was measured by determining the amount of phosphate released from ATP by washed erythrocyte membranes(ghost) during 20 min. incubation. Both total hydrolysis and the amount of hydrolysis that occured in the presence of ouabain were measured. The second measurement yields Mg-ATPase and represents nonspecific ATPase activity of the membranes. The difference between total and Mg-ATPase activity can be attributed to Na-K-ATPase. For the measurement of sodium fluxes, human erythrocytes were preincubated in $^{22}Na$ for 3 hr at $37^{\circ}C$, washed and suspended in a tracer-free medium. The amount of $^{22}Na$ transported out of cells at any time was determined by analysis of supernatant samples taken at various time after addition of the labeled cells to isotope-free medium. The cells and medium were separated and the radioactivity appearing in the medium was measured. From the total radioactivity in the suspension and the radioactivity appearing in the medium at known time, the rate constant for sodium release was computed. The results are summarized as follows: 1) ATP and ATP/ADP were found to increase at every concentration of saponin tested whereas ADP declined at every cone. of saponin. The increase in pyruvate and lactate were observed at every cone, of saponin and thus $NAD^+/NADH$ computed from pyruvate/lactate also increased. Glucose utilization was stimulated by saponin. 2) $Na^+-K^+-ATPase$ activities showed a biphasic response to saponin, first increasing in lower concentration and then decreasing in higher concentration of saponin. 3) The efflux of sodium was significantly increased by saponin in the range of 5 to 10 mg%. The stimulatory effect of saponin on the rate constants for active(ouabain-sensitive) sodium efflux was inhibited by addition of ouabain.

• 한국식품위생안전성학회지
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• 제19권3호
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• pp.151-160
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• 2004

우리나라 고유의 전통발효 식품인 메주로부터 항리스테리아 활성을 나타내는 유산균을 분리한 후 동정한 결과 E. faecium MJ5-14로 명명되었으며, 이들의 박테리오신은 MRS broth 중에 $37^{\circ}C$에서 12-18시간 배양하는 동안 최대의 활성을 나타내었다. L. monocytogenes KCTC 3569와 KCTC 3710 및 L. ivanovii subsp. ivanovii에 대한 박테리오신의 활성은 640 BU/mL이었고, L. innocua ATCC 33090, L. ivanovii ATCC 19119, L. grayi KCTC 3581 및 L. seeliger KCTC 3591에 대한 활성은 1280 BU/mL으로 나타났다. L. monovytogenes KCTC 3569와 E. faecium MJ5-14을 혼합 배양한 경우 L. monocytogenes의 감소 효과는 $25^{\circ}C$이하 보다는 E. faecium MJ5-14의 박테리오신 생산 최적 온도인 $37^{\circ}C$에서 더 높게 나타났으며, E. faecium MJ15-14의 박테리오신 항균 효과는 L. monocytogenes KCTC 3569의 초기 균수와 첨가한 박테리오신의 양에 따라 좌우하는 L. monocytogenes의 초기 균수가 적고, 박테리오신의 첨가량이 많을수록 항균효과가 높아짐을 확인하였다. 또한 박테리오신 70 BU/mL 첨가에 의한 항균 효과는 균 증식이 활발한 대수증식기 중반 이후 보다는 유도기 내지는 대수증식기 초반부에 더 크게 나타났다. 한편, 박테리오신 25 BU/mL을 첨가에 의해서 L. monocytogenes KCTC 3569 새포벽의 일부가 얇아지고, 50 BU/mL 이상의 농도에 의해선 세포막이 파괴되면서 세포 내용물의 유출 현상이 관찰되었다.