• Molecules and Cells
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• 제43권4호
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• pp.350-359
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• 2020

Pathogenic aminoacyl-tRNA synthetases (ARSs) are attractive targets for anti-infective agents because their catalytic active sites are different from those of human ARSs. Mupirocin is a topical antibiotic that specifically inhibits bacterial isoleucyl-tRNA synthetase (IleRS), resulting in a block to protein synthesis. Previous studies on Thermus thermophilus IleRS indicated that mupirocin-resistance of eukaryotic IleRS is primarily due to differences in two amino acids, His581 and Leu583, in the active site. However, without a eukaryotic IleRS structure, the structural basis for mupirocin-resistance of eukaryotic IleRS remains elusive. Herein, we determined the crystal structure of Candida albicans IleRS complexed with Ile-AMP at 2.9 A resolution. The largest difference between eukaryotic and prokaryotic IleRS enzymes is closure of the active site pocket by Phe55 in the HIGH loop; Arg410 in the CP core loop; and the second Lys in the KMSKR loop. The Ile-AMP product is lodged in a closed active site, which may restrict its release and thereby enhance catalytic efficiency. The compact active site also prevents the optimal positioning of the 9-hydroxynonanoic acid of mupirocin and plays a critical role in resistance of eukaryotic IleRS to anti-infective agents.

• 대한장애인치과학회지
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• 제9권1호
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• pp.30-35
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• 2013

The four principles of treatment of odontogenic infection are as follows : (1) removal of the cause, (2) establishment of drainage, (3) institution of antibiotic therapy, and (4) provision of supportive care, including proper rest and nutrition. A separate incision is required to establish drainage, especially in the case of extensive fascial space infections. There are four principle causes for active bleeding in the immediate incision & drainage phase; (1) vascular wall alteration (infection, scurvy, chemicals), (2) disorder of platelet function, (3) thrombocytopenic purpuras, (4) disorders of coagulation (liver disease, anticoagulation drug). If the hemorrhage from incision & drainage site is aggressive, the site must be packed with proper wet gauze and wound closure & drainage dressing are applied. The specific causes of bleeding may be associated with hypoxia, changes in the pH of blood & chemical changes affecting vascular contractility and blood clotting. This is a case report of bleeding control by the circumferential suture & drainage on active bleeding incision & drainage site of temporal space abscess due to advanced odontogenic infection in a multiple medically compromised disabled patient.

• 한국농촌건축학회논문집
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• 제20권1호
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• pp.53-60
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• 2018

The purpose of this study is to present the basic data for the active use of closed schools by analyzing the actual conditions of closed schools in Gyeongnam area. As a method of this study, we examined the population change in Gyeongnam area through the literature survey and the internet site survey, and grasped the characteristics of the policies related to the closed school. Through the homepage survey of the local education financial alerts, we analyzed the contents, location, period after the closure, land area, and total floor area of the closed schools that have been in use or invaluable as of March 2017. As the results, In the case of self-utilization, the utilization rate of educational facilities was overwhelmingly high. In the case of lease utilization, the utilization ratio was higher in the order of education facilities, income increase facilities, and cultural facilities. Overall, 62.8% of the closed schools are located in the base towns (urban areas). The average of the period after the closure was 16.2 years, the land area average was $9,291.1m^2$, and the total area average was $1,153.8m^2$.

• Journal of Trauma and Injury
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• 제30권1호
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• pp.12-15
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• 2017

A 58-year-old male visited our emergency room for multiple traumas from explosion. On initial evaluation, hemopneumoperitoneum with liver laceration (grade 4) and colon perforation was identified. Hemopericardium with cardiac tamponade was also identified. Shrapnel was detected in the right ventricle. Damage control surgery was planned due to condition of hypotension. In operation room, control over bleeding was achieved after sternotomy, pericardiotomy, and laparotomy. Massive transfusion was done during operation. After gauze packing, operation was terminated with temporary closure (TC). Sanguineous fluid was drained profusely. Disseminated intravascular coagulopathy was confirmed through laboratory findings. No extravasation was discovered at hepatic angiogram. On re-operation, there was no active bleeding but oozing from sternotomy site was identified. Bone bleeding was impossible to control. Finally, reoperation was ended after gauze packing and TC all over again. The patient could survive for only a day after re-operation.

• Journal of Trauma and Injury
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• 제26권3호
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• pp.226-228
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• 2013

Elderly patients with acute subdural hematomas have higher mortality and lower functional recovery rates compared with those of other head-injured patients. Early and widely surgical decompression and active intensive care represent the best way to assist these patients. However, abrupt decompression of the hematoma can lead to brain disruption and secondary ischemia in the brain surrounding the craniectomy site. Acute brain swelling and brain extrusion, which take place shortly after decompression, can lead to a catastrophic situation during the operation due to the impossibility of appropriate closure of the dura and scalp. To avoid the deleterious consequences of disruption of brain tissue, we have adopted multiple fenestrations of the dura in a mesh-like fashion and gradual release of subdural clots through the small dural openings that are left open. This is especially important in cases in which there are massive amount of subdural hematomas with small parenchymal lesion and severe midline shifts in elderly patients. Further clinical experiences should be conducted in a more selected series patients to estimate the impact of this technique on morbidity and mortality rates.

• 한국지반공학회논문집
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• 제17권6호
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• pp.47-51
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• 2001

비위생매립지 정비방안은 현장에 매립된 폐기물을 파내어 재활용 여부에 따라 처리방법을 달리하는 능동적 방법과 토질 역학적 안정을 피하여 사면부 불안정으로 야기되는 붕괴 및 폐기물, 침출수, 가스의 외부유출을 방지하는 수동적 방법이 있다. 이 논문에서는 매립지 사면부 붕괴에 따른 침출수 유출을 방지하기 위한 수동적 방법으로서의 지오크리트와 규산소다액의 적용가능성을 조사하였다. 배합비 I (지오크리트:규산소다액=1:3.9)과 배합비 H (1:2.5)의 7일 압축 강도는 5.95kg/$cm^3$, 17.05kg/$cm^3$으로 각각 나타나 매립지 공사 최소 허용강도인 5kg/$cm^3$을 상회하였다. 내산성시험 결과, 배합비 I, II의 압축강도는 급격히 감소하였다. 지오크리트와 규산소다액의 환경 독성을 판단하기 위한 어독성시험 결과, 주변환경에 대한 영향은 없는 것으로 나타났다. 한편 투수시험 결과, 배합비 I, II 모두 매립지 허용기준 $(1.0\times10_{-7}cm/sec)$을 만족하였다.

• 한국조경학회지
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• 제33권1호
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• pp.93-108
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• 2005

Fresh Kills is the largest landfill in the world located in the west side of Staten Island, New York. The landfill served as a storage area for New York City's trash for more than 50 years. After years of civilian and political pressure, state and local legislation decided its closure of landfill operation in Fresh Kills in March 2001. Soon after, Department of City Planning announced a Fresh Kills international design com-petition: 'Landfill to Landscape'. The winning entry was promised to be outline for the redevelopment of the 2,200 acre site which the size of three times Central Park. Forty-eight teams representing more than 200 offices from around world submitted proposals, from which six finalists that mostly led by landscape architects were selected. In December 2001, a jury of architects, landscape architects and city officials unanimously selected Field Operations as the winner. The plan, named Lifescape, visualizes the gradual 20-year transformation of the whole Staten Island into a 'natural lifestyle island' recognizing that Staten Island is home to coastal wetlands that shelter one of the most diverse ecosystems in the New York metropolitan area. It suggested that an ecologically reconstituted Fresh Kills could become the center of integrated parks and greenways system on the island otherwise fragmented. The project will be one of the largest and most ambitious undertakings in the metropolis in years developing a complex web of habitats and parklands on top of mountain of trash. This study tries to achieve two goals: One is to provide general explanations on the project, Lifescape, breaking down to its background, geographical context, design concepts and phased development plan. Another is to introduce the unique and innovative design approaches by Field Operations that are different from a conventional landscape architectural attitude. Since this project was well published through many magazines and newspapers, main focus will be upon aspects that differentiate this project from usual landscape projects. Conceptually Lifescape brought provocative notions on nature/culture relationship and the role of urban park as an active agency rather than just a green rest area. Also this project introduced pioneering graphics like plan collage, diagrammatic plan, phasing diagram and photo montage as vehicles conveying information, imagination and provocation. Witnessing the influence of the project gradually in the field of academic and practice in the States, this study is intended to become a constructive reference to similar landscape projects dealing with large and complex urban context in conjunction with restructure of contemporary city.