• BMB Reports
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• v.33 no.3
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• pp.263-267
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• 2000

We cloned $hda1^+$ (histone deacetylase 1) of fission yeast Schizosaccharomyces pombe. The hda1 of S. pombe was previously reported to encode for an active histone deacetylase (Rundlett et al., 1996; Olsson et al., 1998). The $hda1^+$ is phylogenetically related to the new open reading frame HOS2 of Saccharomyces cerevisiae and only shows a partial homology to the well-known histone deacetylase subclasses, RPD3 and HDA1. A single hda1 mRNA of 1.8 kb was detected at the same level in actively growing and nitrogen-starved cells. When highly over-expressed in S. pombe from an inducible promoter, $hda1^+$ inhibited cell proliferation and caused defects in morphology and cell division. The increased histone deacetylase activity was detected in hdar over-expressing cells. These results suggest that the Hda1p should function on the regulation of cell division possibly by (Allfrey, 1966) direct deacetylation of cytoskeletal (Wade et al., 1997) and cell division regulatory proteins, (Wolffe, 1997) or by controlling their gene expressions.

• Korean Journal of Child Studies
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• v.32 no.6
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• pp.95-106
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• 2011

This study was conducted to better understand the effect of the classroom literacy environment on the young children's vocabulary abilities, and the concepts of print matter. The subjects consisted of 276 children aged 3 to 5 enrolled in 93 classrooms in child-care centers and kindergartens. The Early Language & Literacy Classroom Observation Pre-K (Smith, Brady, & Anastasopoulos, 2008), the Picture Vocabulary Test (Kim, Jang, Yim, & Bae, 1995) and the Concepts About Print (Kim & Kim, 2004) were used for the purposes of this study. The data collected were analyzed by means of ANOVA, and regression analysis. The results indicated that the vocabulary abilities and print-concepts of children aged 3-5 were more likely to increase when a rich classroom environment was provided for the children's literacy development. In particular, it was found to be important for children's language and Literacy development to provide them with an enriched 'language environment' within the classroom, the quality of 'books and book reading', and the active utilization of 'print and early writing' all being important elements of this.

• Journal of the Korean housing association
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• v.27 no.5
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• pp.25-35
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• 2016

he objective of this study was to investigate home network systems presently applied in multi-housing complexes and resident's usage to improve the utilization of these systems and services as well as maintenance methods. Subjects were 27 housing complexes equipped with home network systems in west Pangyo area. The investigation methods of communal network systems were observed and photographed. Unit systems were investigated through photography, interviews, and observation focusing on the utilization of Wall-Pads by visiting one unit of each housing complex. The results are as follows: (1) Most housing complexes that we investigated were built with high-grade IT infrastructure. Also, remote meter reading, electronic security, vehicle access, and building access systems were established. Wall-Pads with similar functions were installed in 23 housing complexes, excluding private rental housing complexes. (2) Even though people were well aware of the need for common systems within their housing complexes, only 10~20% of Wall-Pad menus were used. (3) Low utilization rates of home network stem from Wall-Pad menus which were user-unfriendly, and a lack of user training for the complex's common system and unit system. Therefore, to promote active use of home network systems, the systems must be diversified in accordance with user characteristics. In addition, the Wall-Pad menus should be reorganized to be user-friendly.

• Journal of the Korean Home Economics Association
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• v.46 no.9
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• pp.71-85
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• 2008

This study examined the effects of a picture book literature education program in order to assess preschool children's recognition of and attitude towards picture books. The subjects were 56 five-year-old children, 30 of whom allocated to the experimental group and 26 to the control group. The literature education program lasted for 14 weeks. The control group children participated on the national early childhood education curriculum. Children were interviewed about their concepts, preferences, and attitudes towards picture books. The results indicated that compared to control group, children who participated in this program developed enhanced and richer concepts of picture books, displayed specific preferences, and put forward clearer reasons for their preference. ANCOVA result showed significant overall differences between the two groups. Children who participated in the ptrgram displayed more voluntary and active reading and applied the contents of the picture books to their real life situations.

• Journal of Microbiology
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• v.34 no.2
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• pp.151-157
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• 1996

By PCR amplification using the sequence of the previously cloned shiga-like toxin II DNA, a gene encoding it has been cloned from an isolate of healthy Korean native bovine feces, Escherichia coli KSC109. The nucleotide sequence s included tow open reading frames coding for 319 and 89 amino acids corresponding to A and B subunits, respectively. Comparison of the nucleotide and predicted amino acid sequences of newly cloned gene (slt-II) with those of others in the SLT-II family revealed completely identical homology with SLT-II cloned previously from bacteriophabe DNA of E. coli 933 derived from a patient with hemorrhagic colities. In addition, the sequence homology of SLT-II with SLT-II variant form bovine was more than 95% at both the nucleotide and protein levels. Overexpression of SLT-II recombinant gene by induction with IPTG using an E, coli hostvector, system was conducted and the correctly processed products with active mature form exhibited 1000-fold higher cytotoxycity for Vero cells than that form original strain.

• Electrical & Electronic Materials
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• v.8 no.2
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• pp.229-236
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• 1995

Multimedia has created a system environment that needs a combination of diverse peripherals, faster I/O, and easier configuration. The sheer volume of data one can expect with multimedia hardware and applications storage systems of higher capacity and faster data transfer rate. Unlike the magneto-optical(MO)disk technology which uses bias magnetic field in writing, both the reading and the writing in the phase change (PC) technology are performed only by laser light. In PC optical media, an active layer is reversibly converted between amorphous state and crystalline state by changing irradiation conditions of focused laser beam. Thus, as compared with MO disk, the PC disk has such great advantages that signals can be reproduced by change of reflectance of laser beams in the same manner as the compact disc. The reflectivity of a phase-change spot can be altered in a single pass under the head only through modulation of laser power. The principles and the current status of phase-change optical recording media combined with possible applications are discussed in the present article.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.14 no.2
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• pp.5-12
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• 2003

The knowledge-based society needs creative and active knowledge workers. To meet the need of this modern society, Korea education begins to focus on reading ability of students and project-based school education. The 7th School Education Program requires performance evaluation, optional activites, and extracurricular activites and the school library is a necessary facility for these educational activities. Ministry of Education of Korea made a master plan to rebuild the school library for The 7th School Education Program. The master plan includes the assistance for the school library facility, school library materials, budget, the library staff. And also the master plan includes promotion for school education in the library to develop the school library as the center of school education.

• Proceedings of the Korea Crystallographic Association Conference
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• 2003.05a
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• pp.17-17
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• 2003

tRNA (m¹ G37) methyltransferase (TrmD) catalyze s the trans for of a methyl group from S-adenosyl-L-methionine (AdoMet) to G/sup 37/ within a subset of bacterial tRNA species, which have a residue G at 36th position. The modified guanosine is adjacent to and 3' of the anticodon and is essential for the maintenance of the correct reading frame during translation. We have determined the first crystal structure of TrmD from Haemophilus influenzae, as a binary complex with either AdoMet or S-adenosyl-L-homocysteine (AdoHcy), as a ternary complex with AdoHcy/phosphate, and as an apo form. The structure indicates that TrmD functions as a dimer (Figure 1). It also suggests the binding mode of G/sup 36/G/sup 37/ in the active site of TrmD and catalytic mechanism. The N-terminal domain has a trefoil knot, in which AdoMet or AdoHcy is bound in a novel, bent conformation. The C-terminal domain shows a structural similarity to DNA binding domain of trp or tot repressor. We propose a plausible model for the TrmD₂-tRNA₂ complex, which provides insights into recognition of the general tRNA structure by TrmD (Figure 2).

• Journal of Microbiology and Biotechnology
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• v.24 no.11
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• pp.1525-1535
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• 2014

The xynC gene, which encodes high molecular weight xylanase from Paenibacillus sp. DG-22, was cloned and expressed in Escherichia coli, and its nucleotide sequence was determined. The xynC gene comprised a 4,419bp open reading frame encoding 1,472 amino acid residues, including a 27 amino acid signal sequence. Sequence analysis indicated that XynC is a multidomain enzyme composed of two family 4_9 carbohydrate-binding modules (CBMs), a catalytic domain of family 10 glycosyl hydrolases, a family 9 CBM, and three S-layer homologous domains. Recombinant XynC was purified to homogeneity by heat treatment, followed by Avicel affinity chromatography. SDS-PAGE and zymogram analysis of the purified enzyme identified three active truncated xylanase species. Protein sequencing of these truncated proteins showed that all had identical N-terminal sequences. In the protein characterization, recombinant XynC exhibited optimal activity at pH 6.5 and $65^{\circ}C$ and remained stable at neutral to alkaline pH (pH 6.0-10.0). The xylanase activity of recombinant XynC was strongly inhibited by 1 mM $Cu^{2+}$ and $Hg^{2+}$, whereas it was noticeably enhanced by 10 mM dithiothreitol. The enzyme exhibited strong activity towards xylans, including beechwood xylan and arabinoxylan, whereas it showed no cellulase activity. The hydrolyzed product patterns of birchwood xylan and xylooligosaccharides by thin-layer chromatography confirmed XynC as an endoxylanase.

• Journal of Microbiology and Biotechnology
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• v.24 no.1
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• pp.113-118
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• 2014

Environmental microorganisms are emerging as an important source of new enzymes for wide-scale industrial application. In this study, novel phytase genes were identified from a soil microbial community. For this, a function-based screening approach was utilized for the identification of phytase activity in a metagenomic library derived from an agricultural soil. Two novel phytases were identified. Interestingly, one of these phytases is an unusual histidine acid phosphatase family phytase, as the conserved motif of the active site of PhyX possesses an additional amino acid residue. The second phytase belongs to a new type, which is encoded by multiple open reading frames (ORFs) and is different to all phytases known to date, which are encoded by a single ORF.