• Journal of Microbiology and Biotechnology
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• 제24권2호
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• pp.236-244
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• 2014

The gene encoding ${\alpha}-\small{L}$-arabinofuranosidase (AFase) from Paenibacillus sp. DG-22 was cloned, sequenced, and expressed in Escherichia coli. The AFase gene (abfA) comprises a 1,509 bp open reading frame encoding 502 amino acids with a molecular mass of 56,520 daltons. The deduced amino acid sequence of the gene shows that AbfA is an enzyme consisting of only a catalytic domain, and that the enzyme has significant similarity to AFases classified into the family 51 of the glycosyl hydrolases. abfA was subcloned into the pQE60 expression vector to fuse it with a six-histidine tag and the recombinant AFase (rAbfA) was purified to homogeneity. The specific activity of the recombinant enzyme was 96.7 U/mg protein. Determination of the apparent molecular mass by gel-filtration chromatography indicated that AbfA has a tetrameric structure. The optimal pH and temperature of the enzyme were 6.0 and $60^{\circ}C$, respectively. The enzyme activity was completely inhibited by 1 mM $HgCl_2$. rAbfA was active only towards p-nitrophephenyl ${\alpha}-\small{L}$-arabinofuranoside and exhibited $K_m$ and $V_{max}$ values of 3.5 mM and 306.1 U/mg, respectively. rAbfA showed a synergistic effect in combination with endoxylanase on the degradation of oat spelt xylan and wheat arabinoxylan.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1067-1072
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• 2005

A metagenomic library was constructed using a fosmid vector, and total genomic DNA was extracted directly from soil at Cisolok (hot spring area, Indonesia). This library was composed of 10,214 clones and screened for lipolytic enzyme on tributyrin agar plates. An esterase gene (estMa) was subcloned and sequenced from a positive lipolytic active clone. Esterase EstMa was encoded by a 954-bp open reading frame and showed low ($11-33\%$) amino acid similarity to known esterases. The amino acid sequence analysis demonstrated that the enzyme is a new member of lipolytic enzyme family VI. The estMa gene encodes a preprotein of 317 amino acids with a predicted molecular mass of 34,799 Da. The purified enzyme exhibited optimal activity at $50^{\circ}C$ and pH 6.5. The $K_m,\;and\;V_{max}$ values of EstMa for the hydrolysis of p-nitrophenyl valerate were $45.3\;{\mu}M$ and 4.45 U/mg, respectively.

• Journal of Microbiology and Biotechnology
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• 제24권6호
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• pp.835-842
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• 2014

Haloperoxidase (HPO, E.C.1.11.1.7) is a metal-containing enzyme oxidizing halonium species, which can be used in the synthesis of halogenated organic compounds, for instance in the production of antimicrobial agents, cosmetics, etc., in the presence of halides and $H_2O_2$. To isolate and evaluate a novel non-metal HPO using a culture-independent method, a cassette PCR library was constructed from marine seawater in Japan. We first isolated a novel HPO gene from Pseudomonas putida ATCC11172 by PCR for constructing the chimeric HPO library (HPO11172). HPO11172 showed each single open-reading frame of 828 base pairs coding for 276 amino acids, respectively, and showed 87% similarity with P. putida IF-3 sequences. Approximately 600 transformants screened for chimeric genes between P. putida ATCC11173 and HPO central fragments were able to identify 113 active clones. Among them, we finally isolated 20 novel HPO genes. Sequence analyses of the obtained 20 clones showed higher homology genes with P. putida or Sinorhizobium or Streptomyces strains. Although the HPO A9 clone showed the lowest homology with HPO11172, clones in group B, including CS19, showed a relatively higher homology of 80%, with 70% identy. E. coli cells expressing these HPO chimeric genes were able to successfully bioconvert chlorodimedone with KBr or KCl as substrate.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2003년도 춘계종합학술대회논문집
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• pp.231-236
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• 2003

영상회의 시스템을 인터넷상에서 다양하게 사용하려는 시도가 이루어지고 있다. 이런 부분의 연구는 오디오, 비디오 압축기법, 멀티미디어 데이터의 동기화, 다자간의 영상회의를 지원하기위한 IP multicast의 Mbone의 연구가 활발하게 이루어지고 있고, 통신의 회선속도가 고속화됨에 따라 인터넷에서 영상을 통한 다양한 멀티미디어 서비스가 이루어지고 있다. 개방형 분산 인터넷 통신망 환경에서의 영상회의는 영상회의 데이터인 영상 및 음성보안에 대한 문제가 심각하게 대두된다. 본 논문에서는 영상회의에서 멀티미디어 데이터의 특성에 따른 보안 방법을 제시하고자 한다.

• 한국멀티미디어학회논문지
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• 제6권4호
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• pp.576-582
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• 2003

Because of today's rapid growth of digital contents market and many benefits of electronic book, many people have considerable interest in E-book. Furthermore multifarious consortiums take an active part in standardization of I-book, and many E-book tools have been provided by software manufactures all over the world. E-book tools include editor for production of E-Book, viewer for reading, and the like. Especially in E-book viewer, annotation function has to be Included to put arrangement, summation, recording, comment, emphasis and after comprehension to practical use. In this paper, a E-book viewer with annotation is designed according to the specifications of EBKS, Korean standard. The proposed viewer is aimed to implement in PDA with embedded Linux, but developed in Windows 2000 platform. Because development environment and application environment are different each other, Qt-Library and cross-compiler are used for cross-platform development. The viewer support various functions such as adjusting of font size, hypertext linking, retrieval of specific word, and so forth. And in addition to these basic functions, annotation function is designed for the viewer, which can be used for re-usage and sharing of important information.

• Journal of Microbiology and Biotechnology
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• 제10권2호
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• pp.221-226
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• 2000

Cephalosporin-C deacetylase (CAH) catalyzes the deacetylation of cephalosporin derivatives. A novel gene encoding the CAH from Bacillus sp. KCCM10143 was cloned and sepuenced. The uncleotide sequence contained an open reading frame encoding a polypeptide consisting of 217 amino acids and a molecular weight of 24 kDa which was in good agreement with the value obtained by sodium dodecylsulfate-polyacrylamide gel electrophoresis. An expression plasmid was constructed by inserting the CAH gene into the region of the pTrc99A expression vector. An active from of the CAH protein was expressed in the soluble fraction obtained after cell disruption. in fermentation using a 5-1 jar fementer, the transformant E. coli JM109 (pDST654) produced 4.12 U of CAH per ml of culture during 16 h of incubation.

• 수산해양교육연구
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• 제17권1호
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• pp.35-47
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• 2005

The purpose of this study is to investigate various kinds of discipline problems in middle school classrooms, and the differences in severity and frequency of the classroom discipline problems between male and female students' classrooms in middle schools. This study shows that the discipline problems in the middle school classrooms include using a cellular phone, chattering, sleeping, chewing gum, reading comic books, asking useless questions, challenging a teacher, fighting, calling names, skipping class, throwing trash in the classroom, breaking classroom equipment, creating disorder, losing materials, playing with a ball in the classroom, gambling, and so on. Generally, male students recognized the severity of the classroom discipline problems more than the female students. However, it is shown that the frequency of discipline problems in male students' classrooms is higher than that in female students' classrooms. From the results of this study, male students have more active discipline problems (playing with a ball in the classroom, throwing trash in the classroom, coming to class late, gambling, cutting in line, and so on). On the other hand, female students have more passive discipline problems (making a noise by using a cellular phone, asking useless questions, sending messages to a classmate, studying other subjects, scattering, and so on).

• 대한임베디드공학회논문지
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• 제1권2호
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• pp.90-103
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• 2006

There are many file systems in various operating systems. Those are usually designed for server environments, where the common cases are usually 'multiple active users', 'great many small files' And they assume a big main memory to be used as buffer cache. So the existing file systems are not suitable for resource hungry embedded systems that process multimedia data streams. In this study, we designed and implemented a new file system which efficiently stores and retrieves multimedia data steams. The proposed file system has a very simple disk layout, which guarantees a quick disk initialization and file system recovery. And we introduced a new indexing-scheme, called the time-based indexing scheme, with the file system. With the indexing scheme, the file system maintains the relation between time and the location for all the multimedia streams. The scheme is useful in searching and playing the compressed multimedia streams by locating exact frame position with given time, resulting in reduction of CPU processing and power consumption. The proposed file system and its APIs utilizing the time-based indexing schemes were implemented firstly on a Linux environment, though it is operating system independent. In the performance evaluation on a real DVR system, which measured the execution time of multi-threaded reading and writing, we found the proposed file system is maximum 38.7% faster than EXT2 file system.

• 대한간호학회지
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• 제8권2호
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• pp.15-23
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• 1978

This study was conducted to see the present situation of rest in a military hospital. 120 medical patients but acute af critical cases were interviewed by the investigators during the period of August 20 to September 10, 1978. The questionnaire was consisted of questions regarding the general characteristics of tile respondents, rest methods and nursing activities inducing them to rest. 1, Of the rest methods, tying down on the bed comfortably placed first at 29.1 percent, followed by sleeping at 20,1 percent, reading at 16.8 percent, taking a moderate exercise, being free from their concerns of diseases & surrounding matters at 11.7 percent, respectively, playing the game of Badook or Chess at 6.7 percent , others at 3.9 percent in that order. 2. They mainly utilized the Lying down method for comfort & rest, but there were some differences depending on their educational backgrounds. 3. Some differences were observed in the methods of rest by the religions. 4. The differences between diseases af rest methods except bed rest af sleeping were observed. 5. Of the nursing activities inducing them to rest, counseling stood first at 43.3 percent, followed by teaching at 28.4 percent, relief of pain af discomfort at 20 percent, active attitudes of nurses to help them at 8.3 percent in that order. 6. There was no significant difference between the level of education or religions and nursing activities inducing them to rest. 7. Nursing activities which induced comfort ＆ rest by diseases were mainly counseling & teaching.

• 대한간호학회지
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• 제2권1호
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• pp.175-188
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• 1971

More and Tore emphasis is put on moral treatment in the care of psychiatric patients. Methods employed for moral treatment rake use of therapeutic activity for the mentally ill patient. In some psychiatric hospitals all of the therapeutic activities are categorized as occupational therapy, while in others these activities are departmentally categorized -recreational, musical, occupational and bibliotherapy. All of these constitute separate departmental therapies. By research, it is known at this time, that the above activities have been of great help in personality integration, reeducation and early recovery of patients, as well as in their social adjustment after discharge. The goal of these therapeutic activities incorporate the goals set by healthier interpersonal relationship and psychotherapy. Put the therapeutic activities require a prolonged time of therapy for effectiveness, economic support, special trained personnel, careful planning, guidance and proper evaluation. Therefore this treatment is a very complicated one. But it may be the most profitable therapy for certain patients. One true fact about this treatment is, that simple recreation, occupation, music, reading, etc., make the patients life in the ward more interesting and have a good effect on the interpersonal relationship between patients. Thus it has been proved that this kind of treatment demands the active and full participation of the personnel and the full support of the hospital administration.