• Journal of Nutrition and Health
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• v.36 no.4
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• pp.382-388
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• 2003

(-)-Epigallocatechin-3-gallate (EGCG) is a polyphenolic compound found in peen tea leaves, and has been known to be one of the most potent catechin species which inhibits cell growth most possibly through an apoptotic cell death. We investigated the apoptotic activity of (-)-EGCG on the human myeloid leukemia cell line, HL-60. Our results of MTT test indicated that (-)-EGCG had a significant antiproliferation effect in HL-60 cells with $IC_{50}$/ (50% inhibition concentration) value of 65 $\mu$M. Giemsa statining of HL-60 cells treated with (-)-EGCG (100 $\mu$M) for 6hrs showed a typical apoptosis-specific morphological change including shrinkage of the cytoplasm, membrane blobbing and compaction of the nuclear chromatin. The DNA fragmentation was observed from the agarose gel electrophoresis of cells treated with (-)-EGCG for 3hrs or longer, and was progressed to a greater degree as treatment time increases. Treatment of the cells with (-)-EGCG (100 $\mu$M) resulted in a rapid release of mitochondrial cytochrome c into the cytosol, and a subsequent cleavage of caspase-3 to an active form in a treatment-time dependent manner. (-)-EGCG (100 $\mu$M) also stimulated proteolytic cleavage of poly-(ADP-ribose) polymerase (PARP) to an active form in HL-60 cells. Tlken together, (-)-EGCG appears to induce the apoptosis in human myeloid leukemia cells via a caspase-dependent pathway. These results suggest the possible application of (-)-EGCG, the major active compound in green tea, as an antiproliferative agent for cancer prevention.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.41 no.4
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• pp.39-43
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• 2004

A typical theme of active vision systems is gaze-fixing of a camera. Here gaze-fixing of a camera means by steering orientation of a camera so that a given point on the object is always at the center of the image. For this we need to combine a function to analyze image data and a function to control orientation of a camera. This paper presents an algorithm for gaze-fixing of a camera where image analysis and orientation control are designed in a frame. At this time, for avoiding difficulties in implementing and aiming for real-time applications we design the algorithm to be a simple closed-form without using my information related to calibration of the camera or structure estimation.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.386.1-386.1
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• 2014

The ionic polymer-metal composite (IPMC), a type of electro-active polymer material, has received enormous interest in various fields such as robotics, medical sensors, artificial muscles because it has many advantages of flexibility, light weight, high displacement, and low voltage activation, compare to traditional mechanical actuators. Mostly noble metal materials such as gold or platinum were used to form the electrode of an IPMC by using electroless plating process. Furthermore, carbon-based materials, which are carbon nanotube (CNT) and reduced graphene-CNT composite, were used to alter the electrode of IPMC. To form the electrode of IPMC, we employ the synthesized graphene on copper foil by chemical vapor deposition method and use the transfer process by using a support of PET/silicone film. The properties of graphene were evaluated by Raman spectroscopy, UV/Vis spectroscopy, and 4-point probe. The structure and surface of IPMC were analyzed via field emission scanning electron microscope. The fabricated IPMC performance such as displacement and operating frequency was measured in underwater.

• The Korean Journal of Food And Nutrition
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• v.3 no.2
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• pp.123-132
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• 1990

Sweet potato $\beta$-amylase is a tetrameric enzyme consisting of four identical polypeptide chains with a molecular weight of 5.6$\times$104, though most of the other $\beta$-amylases are monomeric enzymes. But, the relationship between subunit structure and catalytic function of the enzyme is not known. This study was done to know what the function of the subunit structure of the enzyme is. We obtained the monomer from the enzyme by the treatment of SDS, alkali pH buffer and urea. But the monomer had not activity. We tried to prepare the active monomer from the enzyme by the modification with periodate-oxidized soluble starch , In the result, we succeeded in isolating an active monomer as an oxidized soluble starch-conjugated form The active monomer had 57% of the original activity, 13.2% of the sugar and the molecular weight was estimated to be 5.4$\times$104. This results suggest that the tetrameric form of the enzyme is a most stable one and exists in nature, and the subunit structure of the enzyme Plays an important role in stabilization but not catalytic function.

• International Journal of Oral Biology
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• v.42 no.2
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• pp.71-78
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• 2017

BMP-2 is a well-known TGF-beta related growth factor, having a significant role in bone and cartilage formation. It has been employed to promote bone formation in some clinical trials, and to differentiate mesenchymal stem cells into osteoblasts. However, it is difficult to obtain this protein in its soluble and active form. hBMP-2 is expressed as an inclusion body in the bacterial system. To continuously supply hBMP-2 for research, we optimized the refolding of recombinant hBMP-2 expressed in E. coli, and established an efficient method by using detergent and alkali. Using a heparin column, the recombinant hBMP-2 was purified with the correct refolding. Although combinatorial refolding remarkably enhanced the solubility of the inclusion body, a higher yield of active dimer form of hBMP-2 was obtained from one-step refolding with detergent. The refolded recombinant hBMP-2 induced alkaline phosphatase activity in mouse myoblasts, at $ED_{50}$ of 300-480ng/ml. Furthermore, the expressions of osteogenic markers were upregulated in hPDLSCs and hDPSCs. Therefore, using the process described in this study, the refolded hBMP-2 might be cost-effectively useful for various differentiation experiments in a laboratory.

• Journal of Microbiology and Biotechnology
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• v.25 no.2
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• pp.247-254
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• 2015

In this presentation, I describe the expression and purification of the recombinant liver X receptor β-ligand binding domain proteins in E. coli using a commercially available double cistronic vector, pACYCDuet-1, to express the receptor heterodimer in a single cell as the soluble form. I describe here the expression and characterization of a biologically active heterodimer composed of the liver X receptor β-ligand binding domain and retinoid X receptor α-ligand binding domain. Although many of these proteins were previously seen to be produced in E. coli as insoluble aggregates or "inclusion bodies", I show here that as a form of heterodimer they can be made in soluble forms that are biologically active. This suggests that co-expression of the liver X receptor β-ligand binding domain with its binding partner improves the solubility of the complex and probably assists in their correct folding, thereby functioning as a type of molecular chaperone.

• Journal of Sericultural and Entomological Science
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• v.6
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• pp.9-17
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• 1966

The ratio of form and character in the text generation of silkworms which were double copulated between home race copulation and hetero race copulation in crossing with two males of different races for female(double crossing) are different according to the copulating time, copulating order and sperm activities. But the general tendencies are as follows; 1. During two hour's double copulation, sufficiently ejaculating time, the fertilization percentage of hetero lace copulation are higher than that of homo race, but in case of double copulation with plain and normal marked silkworms showed opposite results. The fertilization percentage of homo race copulation are equal or higher compare with that of hetero race copulation. 2. The form and character of the next generation were largely effected by copulating order, so the primary copulating moths are more effected in the next generation than the secondary moths. 3. The active sperms were more fertilized than non-active sperms in the double copulation.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1994.10a
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• pp.397-401
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• 1994

This paper proposes an identification method for a thin plate where multiple actuators and sensors are bonded. Since a thin plate has small damping ratios of all modes, each mode can be identified seperately with a bandpass filter for each modal signal. With the bandpass filter and the characteristics of the plate, the Multi-Input Multi-Output (MIMO) model of the plate can be converted to several Multi-Input Single-Output(MISO) models of second order linear difference equations of the modes. Parameters for each mode are obtained by using the Least Square method. Form there MISO models, the MIMO model is obtained in the form of the state space. Experiments were performed for an all-clamped plate with two pairs of piezoelectric actuators and sensors. The outputs of the identified model and the experimental data match well.

• Convergence Security Journal
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• v.22 no.1
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• pp.135-140
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• 2022

This study aims to analyze exercise cases and issues using smart devices and technologies, and to present the development direction of a smart exercise environment suitable for the wellness life of active seniors with high activity and economic power unlike the existing silver generation. In the fitness industry, the subscription economy that regularly receives or uses necessary exercise tools, services, and digital content is expanding, and business models based on hardware sales and content subscription continue to emerge. In order to have value competitiveness as a platform that provides active seniors with integrated exercise services for health care, not only fitness centers, but also home training exercise equipment, fitness-related applications, and smart wearable device markets should be organically connected to form an expanded total platform. In order to have value competitiveness as a platform that provides active seniors with integrated exercise services for health care, not only fitness centers, but also home training exercise equipment, fitness-related applications, and smart wearable device markets should be organically connected to form an expanded total platform. The linkage of the digital healthcare function, which provides real-time changes to exercise programs based on continuous monitoring and feed back through wearable devices before, after, and during exercise by receiving and selecting exercise programs suitable for individual health status, is the differentiating factor in the smart fitness model.

• The KIPS Transactions:PartB
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• v.10B no.6
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• pp.599-610
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• 2003

The Web plays an important role as information source and most Web applications are document-centric. A document implies an intention of its own designer, which can be utilized actively in automation of business processes. Through an understanding of an intrinsic nature of a document function, we can see a document as an executable computer program in a special case. For this approach, we propose an active document model that is composed of form, knowledge base, rules, and queries. For reusability and interoperability of a document, each component of the proposed model is uniformly represented in XML. The proposed active document not only plays a passive role in providing user interfaces, but also is a document that a machine can infer and process with reading a procedure of document processing and business rules intended by document designers. Through this approach, document can interact with machines and can cooperate with other applications. For applicability of our active document, we show a case study for the processing of purchase orders in a B2B e-Commerce system. This paper is expected to provide the framework of accelerating the development of intelligent applications through our approach regards form document as a computer program. In short, the proposed active document contains knowledge representation and processing method, consequently our document will play an important role in providing a concept of document of pursuing in Semantic Web.