• 한국정보과학회논문지:소프트웨어및응용
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• 제30권3_4호
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• pp.347-357
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• 2003

웹은 중요한 정보인천이며, 대부분의 웹 응용프로그램은 HTML 작성된 서식문서를 기반으로 한다. 이러한 HTML 기반의 서식문서는 사용자 인터페이스를 제공하는 역할을 할 뿐, 문서서식 설계자가 지향하는 업무처리 절차나 로직을 내포하지는 않는다 그러나 서식문서에는 그것에 대한 처리방법이 함축되어 있고. 이렇게 내재된 전차적 지신을 업무치리 과정의 자동화에 적극적으로 활용할 수 있다. 이에, 본 논문에서는 서신문서에 기반한 업무치리 전차를 자동화시키기 위해 인지과학적인 측면에서 문서의 능동성을 파악하였나. 이를 통해 능동문서(Active Documents)에 대한 새로운 개념과 그 적용 가능성을 제시하고자 한다. 이 능동문서는 문서에 함축되어 있는 업무규칙과 문서처리의 자동화를 지원하기 위한 선언적 지식을 문서 사제에 내포하고 있다. 또한, 본 논문에서는 제시된 능동문서를 처리하기 위한 프레임워크를 제안한다. 제안된 프레임워크는 크게 생성단계와 실행단계로 구성된다. 본 논문에서 제안한 프레임워크의 유용성을 보이기 위해, 인터넷 구매 시스템의 구매 요청서 처리에 능동문서를 적용한 ActiveForm이라는 원형시스템을 설계하고 구현하였다. 그 결과, 본 논문은 서식설계자의 지식이 Prolog로 명시적으로 표현되어 있는 능동문서를 추론엔진에서 처리함으로써 인터넷 응용프로그램의 지능화에 기여할 수 있음을 확인하였다.

• Journal of information and communication convergence engineering
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• 제6권2호
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• pp.198-203
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• 2008

An Intelligent optimal control system design algorithm in active suspension equipment adopting linear matrix inequalities control system design theory with representing by descriptor system form is presented. The validity of the linear matrix inequalities intelligent decentralized control system design with representing by descriptor system form in active suspension system through the numerical examples is also investigated.

• 한국안전학회지
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• 제28권1호
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• pp.74-80
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• 2013

This study describes structural reliability analysis of actively-controlled structure for which random vibration analysis is incorporated into the first-order reliability method (FORM) framework. The existing approaches perform the reliability analysis based on the RMS response, whereas the proposed study uses the peak response for the reliability analysis. Therefore, the proposed approach provides us a meaningful performance measure of the active control system, i.e., realistic failure probability. In addition, it can deal with the uncertainties in the system parameters as well as the excitations in single-loop reliability analysis, whereas the conventional random vibration analysis requires double-loop reliability analysis; one is for the system parameters and the other is for stochastic excitations. The effectiveness of the proposed approach is demonstrated through a numerical example where the proposed approach shows fast and accurate reliability (or inversely failure probability) assessment results of the dynamical active control system against random seismic excitations in the presence of parametric uncertainties of the dynamical structural system.

• Journal of Microbiology and Biotechnology
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• 제24권5호
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• pp.719-723
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• 2014

Caspases are a family of cysteine proteases that play an important role in the apoptotic pathway. Caspase-6 is an apoptosis effector that cleaves a variety of cellular substrates. The active form of the enzyme is required for use in research. However, it has been difficult to obtain sufficient quantities of active caspase-6 from Escherichia coli. In the present study, we constructed a caspase-6 with a 23-amino-acid deletion in the pro-domain. This engineered enzyme was expressed as a soluble protein in E. coli and was purified using affinity resin. In vitro enzyme assay and cleavage analysis revealed that the engineered active caspase-6 protein had characteristics similar to those of wild-type caspase-6. This novel method can be a valuable tool for obtaining active caspase-6 that can be used for screening caspase-6-specific substrates, which in turn can be used to elucidate the function of caspase-6 in apoptosis.

• Structural Engineering and Mechanics
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• 제30권1호
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• pp.1-20
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• 2008

The dynamic characteristics of the passive, semi-active, and active tuned-liquidcolumn dampers (or TLCDs) are studied in this paper. The design of the latter two are based on the first one. A water-head difference (or simply named as water head in this paper) of a passive TLCD is pre-set to form the so-called semi-active one in this paper. The pre-set of water head is released at a proper time instant during an earthquake excitation in order to enhance the vibration reduction of a structure. Two propellers are installed along a shaft inside and at the center of a passive TLCD to form an active one. These two propellers are driven by a servo-motor controlled by a computer to provide the control force. The seismic responses of a five-story shear building with a passive, semiactive, and active TLCDs are computed for demonstration and discussion. The responses of this building with a tuned mass damper (or TMD) are also included for comparison. The small-scale shaking-table experiments of a pendulum-like system with a passive or active TLCD to harmonic and seismic excitations are conducted for verification.

• Archives of Pharmacal Research
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• 제17권5호
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• pp.318-322
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• 1994

Upon gel filtration, the commercial bovine milk xanthine oxidase preparation was fractionated into two preparations showing enzyme activity. Native polyacrylamide gel electrophoresis showed that one was in a dimeric form and the other was a monomer having molecular weight of 150 kDa. It was also found that this commercial enzyme existed mostly in an active monomeric form without loss of enzyme activity. The rabbit antisera produced against two enzyme preparations cross-reacted well each other. In SDS-polyacrylamide gtel electro-phoresis, however, both enzyme preparations yielded two smaller protein bands below 150 kDa, which appeared to bind with both antisera with high affinity but not to retain enzyme activity. It implies that bovine milk xanthine oxidase can lose its activity when monomeric subunit is further degraded.

• 한국지진공학회:학술대회논문집
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• 한국지진공학회 2003년도 추계 학술발표회논문집
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• pp.387-394
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• 2003

A compromise between passive and active control systems has been developed recently in the form of semi-active control systems. Semi-active control systems maintain the reliability of passive control systems while taking advantage of the adjustability of an active control system. This paper presents the results of an experimental study to evaluate the performance of a semi-active orificed fluid damper. The semi-active orificed fluid damper considered is a two-stage damper with normally open solenoid valve. Through a series of experimental tests, characteristics and performance of the damper is investigated.

• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.840-846
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• 2007

Human prostate-specific antigen(PSA), a 33 kDa serine protease with comprehensive homology to glandular kallikrein, is secreted from prostatic tissue into the seminal fluid and enters into the circulation. The level of PSA increases in the serum of patients with prostatic cancer and hence is widely employed as a marker of the disease status. In particular, an enzymatically active PSA that is a form cleaved at the N-terminal seven-amino-acids prosequence, APLILSR, of proPSA may play an important roll in the progression of prostate cancer. Thus, the presence of the active form would selectively discriminate the cancer from benign prostatic hyperplasia. In this study, we developed a convenient purification method for the acquisition of active PSA and proPSA. Recombinant proPSA and active PSA were expressed directly in Escherichia coli, easily and efficiently isolated from inclusion bodies, refolded, and purified. Moreover, the enzymatic activity of the recombinant active PSA was confirmed as serine protease using chromogenic chymotrypsin substrate. This purified active PSA could be further applied to scrutinize the biological or conformational characteristics of the protein and to develop specific diagnostic and/or therapeutic agents against prostate cancer.

• The Korean Journal of Physiology and Pharmacology
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• 제2권3호
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• pp.385-393
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• 1998

Human neutrophil elastase (HNElastase, EC 3.4.21.37), a causative factor of inflammatory diseases, was purified by Ultrogel AcA54 gel filtration and CM-Sephadex ion exchange chromatography. HNElastase was inhibited by phenylbutazone in a concentration dependent manner up to 0.4 mM, but as the concentration increased, the inhibitory effect gradually diminished. Binding of phenylbutazone to the human neutrophil elastase caused strong Raman shifts at 200, 440, and 1194 $cm^{-1}$. The peak at 1194 $cm^{-1}$ might be evidence of the presence $of\;-N=N-{\Phi}$ radical. The core area of the elastase, according to the visual molecular model of human neutrophil elastase, was structurally stable. A deeply situated active center was at the core area surrounded by hydrophobic amino acids. Directly neighboring the active site was one positively charged atom and two atoms carrying a negative charge, which enabled the enzyme and the drug to form a strong interaction. Phenylbutazone may form a binding, similar to a key & lock system to the atoms carrying opposite charges near the active site of the enzyme molecule. Furthermore, the hydrophobicity of the surrounding amino acid near the active site seemed to enhance the binding strength of phenylbutazone. Binding of phenylbutazone near the active site may cause masking of the active site, preventing the substrate from approaching the active site and inhibiting elastase activity.

• Structural Engineering and Mechanics
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• 제48권3호
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• pp.367-382
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• 2013

Different types of long slender pile shall buckle with weak soil and liquefied stratum surrounded. Different from considering single side earth pressure, it was suggested that the lateral earth pressure can be divided into two categories while buckling: the earth pressure that prevent and promotes the lateral movement. Active and passive earth pressure calculation model was proposed supposing earth pressure changed linearly with displacement considering overlying load, shaft resistance, earth pressure at both sides of the pile. Critical buckling load calculation method was proposed based on the principle of minimum potential energy quoting the earth pressure calculation model. The calculation result was contrasted with the field test result of small diameter TC pile (Plastic Tube Cast-in-place pile). The fix form could be fixed-hinged in the actual calculation assuring the accuracy and certain safety factor. The contributions of pile fix form depend on the pile length for the same geological conditions. There exists critical friction value in specific geological conditions that the side friction has larger impact on the critical buckling load while it is less than the value and has less impact with larger value. The buckling load was not simply changed linearly with friction. The buckling load decreases with increased limit active displacement and the load tend to be constant with larger active displacement value; the critical buckling load will be the same for different fix form for the small values.