• Journal of KIISE:Software and Applications
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• v.30 no.3_4
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• pp.347-357
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• 2003

The web is an important source of information and most of Web applications are based on form documents in HTML-based form documents only play a role as user interfaces, and they do not involve the procedures or rules if business process which form document designers assume. However, from documents imply methods for treating documents, and these embedded procedural knowledge can be utilized.actively in automation of business process. In this respect, we Investigate the activeness of documents with cognitive science to automate business processes based on from documents. Through this, we have a new concept and applicability of active documents. Our active documents include business rules and declarative knowledge to support the automation of document processing. Also, we propose a processing framework for the active documents. The framework has two phases: build-time and run-time. in order to demonstrate the usefulness of the proposed framework, a prototype called ActiveForm is designed and implemented for requisition processing them in an inference engine can enhance the intelligence of Internet applications.

• Journal of information and communication convergence engineering
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• v.6 no.2
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• pp.198-203
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• 2008

An Intelligent optimal control system design algorithm in active suspension equipment adopting linear matrix inequalities control system design theory with representing by descriptor system form is presented. The validity of the linear matrix inequalities intelligent decentralized control system design with representing by descriptor system form in active suspension system through the numerical examples is also investigated.

• Journal of the Korean Society of Safety
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• v.28 no.1
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• pp.74-80
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• 2013

This study describes structural reliability analysis of actively-controlled structure for which random vibration analysis is incorporated into the first-order reliability method (FORM) framework. The existing approaches perform the reliability analysis based on the RMS response, whereas the proposed study uses the peak response for the reliability analysis. Therefore, the proposed approach provides us a meaningful performance measure of the active control system, i.e., realistic failure probability. In addition, it can deal with the uncertainties in the system parameters as well as the excitations in single-loop reliability analysis, whereas the conventional random vibration analysis requires double-loop reliability analysis; one is for the system parameters and the other is for stochastic excitations. The effectiveness of the proposed approach is demonstrated through a numerical example where the proposed approach shows fast and accurate reliability (or inversely failure probability) assessment results of the dynamical active control system against random seismic excitations in the presence of parametric uncertainties of the dynamical structural system.

• Journal of Microbiology and Biotechnology
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• v.24 no.5
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• pp.719-723
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• 2014

Caspases are a family of cysteine proteases that play an important role in the apoptotic pathway. Caspase-6 is an apoptosis effector that cleaves a variety of cellular substrates. The active form of the enzyme is required for use in research. However, it has been difficult to obtain sufficient quantities of active caspase-6 from Escherichia coli. In the present study, we constructed a caspase-6 with a 23-amino-acid deletion in the pro-domain. This engineered enzyme was expressed as a soluble protein in E. coli and was purified using affinity resin. In vitro enzyme assay and cleavage analysis revealed that the engineered active caspase-6 protein had characteristics similar to those of wild-type caspase-6. This novel method can be a valuable tool for obtaining active caspase-6 that can be used for screening caspase-6-specific substrates, which in turn can be used to elucidate the function of caspase-6 in apoptosis.

• Structural Engineering and Mechanics
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• v.30 no.1
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• pp.1-20
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• 2008

The dynamic characteristics of the passive, semi-active, and active tuned-liquidcolumn dampers (or TLCDs) are studied in this paper. The design of the latter two are based on the first one. A water-head difference (or simply named as water head in this paper) of a passive TLCD is pre-set to form the so-called semi-active one in this paper. The pre-set of water head is released at a proper time instant during an earthquake excitation in order to enhance the vibration reduction of a structure. Two propellers are installed along a shaft inside and at the center of a passive TLCD to form an active one. These two propellers are driven by a servo-motor controlled by a computer to provide the control force. The seismic responses of a five-story shear building with a passive, semiactive, and active TLCDs are computed for demonstration and discussion. The responses of this building with a tuned mass damper (or TMD) are also included for comparison. The small-scale shaking-table experiments of a pendulum-like system with a passive or active TLCD to harmonic and seismic excitations are conducted for verification.

• Archives of Pharmacal Research
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• v.17 no.5
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• pp.318-322
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• 1994

Upon gel filtration, the commercial bovine milk xanthine oxidase preparation was fractionated into two preparations showing enzyme activity. Native polyacrylamide gel electrophoresis showed that one was in a dimeric form and the other was a monomer having molecular weight of 150 kDa. It was also found that this commercial enzyme existed mostly in an active monomeric form without loss of enzyme activity. The rabbit antisera produced against two enzyme preparations cross-reacted well each other. In SDS-polyacrylamide gtel electro-phoresis, however, both enzyme preparations yielded two smaller protein bands below 150 kDa, which appeared to bind with both antisera with high affinity but not to retain enzyme activity. It implies that bovine milk xanthine oxidase can lose its activity when monomeric subunit is further degraded.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2003.09a
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• pp.387-394
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• 2003

A compromise between passive and active control systems has been developed recently in the form of semi-active control systems. Semi-active control systems maintain the reliability of passive control systems while taking advantage of the adjustability of an active control system. This paper presents the results of an experimental study to evaluate the performance of a semi-active orificed fluid damper. The semi-active orificed fluid damper considered is a two-stage damper with normally open solenoid valve. Through a series of experimental tests, characteristics and performance of the damper is investigated.

• Journal of Microbiology and Biotechnology
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• v.17 no.5
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• pp.840-846
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• 2007

Human prostate-specific antigen(PSA), a 33 kDa serine protease with comprehensive homology to glandular kallikrein, is secreted from prostatic tissue into the seminal fluid and enters into the circulation. The level of PSA increases in the serum of patients with prostatic cancer and hence is widely employed as a marker of the disease status. In particular, an enzymatically active PSA that is a form cleaved at the N-terminal seven-amino-acids prosequence, APLILSR, of proPSA may play an important roll in the progression of prostate cancer. Thus, the presence of the active form would selectively discriminate the cancer from benign prostatic hyperplasia. In this study, we developed a convenient purification method for the acquisition of active PSA and proPSA. Recombinant proPSA and active PSA were expressed directly in Escherichia coli, easily and efficiently isolated from inclusion bodies, refolded, and purified. Moreover, the enzymatic activity of the recombinant active PSA was confirmed as serine protease using chromogenic chymotrypsin substrate. This purified active PSA could be further applied to scrutinize the biological or conformational characteristics of the protein and to develop specific diagnostic and/or therapeutic agents against prostate cancer.

• The Korean Journal of Physiology and Pharmacology
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• v.2 no.3
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• pp.385-393
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• 1998

Human neutrophil elastase (HNElastase, EC 3.4.21.37), a causative factor of inflammatory diseases, was purified by Ultrogel AcA54 gel filtration and CM-Sephadex ion exchange chromatography. HNElastase was inhibited by phenylbutazone in a concentration dependent manner up to 0.4 mM, but as the concentration increased, the inhibitory effect gradually diminished. Binding of phenylbutazone to the human neutrophil elastase caused strong Raman shifts at 200, 440, and 1194 $cm^{-1}$. The peak at 1194 $cm^{-1}$ might be evidence of the presence $of\;-N=N-{\Phi}$ radical. The core area of the elastase, according to the visual molecular model of human neutrophil elastase, was structurally stable. A deeply situated active center was at the core area surrounded by hydrophobic amino acids. Directly neighboring the active site was one positively charged atom and two atoms carrying a negative charge, which enabled the enzyme and the drug to form a strong interaction. Phenylbutazone may form a binding, similar to a key & lock system to the atoms carrying opposite charges near the active site of the enzyme molecule. Furthermore, the hydrophobicity of the surrounding amino acid near the active site seemed to enhance the binding strength of phenylbutazone. Binding of phenylbutazone near the active site may cause masking of the active site, preventing the substrate from approaching the active site and inhibiting elastase activity.

• Structural Engineering and Mechanics
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• v.48 no.3
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• pp.367-382
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• 2013

Different types of long slender pile shall buckle with weak soil and liquefied stratum surrounded. Different from considering single side earth pressure, it was suggested that the lateral earth pressure can be divided into two categories while buckling: the earth pressure that prevent and promotes the lateral movement. Active and passive earth pressure calculation model was proposed supposing earth pressure changed linearly with displacement considering overlying load, shaft resistance, earth pressure at both sides of the pile. Critical buckling load calculation method was proposed based on the principle of minimum potential energy quoting the earth pressure calculation model. The calculation result was contrasted with the field test result of small diameter TC pile (Plastic Tube Cast-in-place pile). The fix form could be fixed-hinged in the actual calculation assuring the accuracy and certain safety factor. The contributions of pile fix form depend on the pile length for the same geological conditions. There exists critical friction value in specific geological conditions that the side friction has larger impact on the critical buckling load while it is less than the value and has less impact with larger value. The buckling load was not simply changed linearly with friction. The buckling load decreases with increased limit active displacement and the load tend to be constant with larger active displacement value; the critical buckling load will be the same for different fix form for the small values.