• 대한수학교육학회지:학교수학
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• 제4권2호
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• pp.193-204
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• 2002

Performance assessment has been introduced to school education as an alternative measure of the former educational assessment which put much emphasis on the result rather than the process of learning, memorization than pursuit of knowledge, and passive than active study. As for the subject of mathematics, the change of the assessment came to replace multiple choice tests with descriptive- and statement-type tests. This means animprovement on the testing system, focusing on the process of finding out the answer. The main focus, however, is still on the intellectual domain without paying due attention to the emotional domain of mathematics education. The previous studies on the assessment of emotional domain In mathematics have shown that there are stumbling blocks in the application of the assessment, such as the disputes on the reliability, objectivity, and fairness as well as the complicated procedure of applying the results to school records. The lack of the development and supply of the appropriate assessment tools have also been pointed out. Therefore, this study has been carried out with the intention of establishing an applicable standard of assessment on the emotional domain of high school matematics. As a result, detailed standards of performance assessment, which adopt oral examination, discussion, observation, and report have been developed. The problems which are likely to emerge In the course of the application of the newly developed assessment are under study as a continuing research project.

• Journal of Microbiology and Biotechnology
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• 제26권2호
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• pp.226-232
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• 2016

Dihydrodipicolinate reductase is an enzyme that converts dihydrodipicolinate to tetrahydrodipicolinate using an NAD(P)H cofactor in L-lysine biosynthesis. To increase the understanding of the molecular mechanisms of lysine biosynthesis, we determined the crystal structure of dihydrodipicolinate reductase from Corynebacterium glutamicum (CgDapB). CgDapB functions as a tetramer, and each protomer is composed of two domains, an Nterminal domain and a C-terminal domain. The N-terminal domain mainly contributes to nucleotide binding, whereas the C-terminal domain is involved in substrate binding. We elucidated the mode of cofactor binding to CgDapB by determining the crystal structure of the enzyme in complex with NADP⁺ and found that CgDapB utilizes both NADH and NADPH as cofactors. Moreover, we determined the substrate binding mode of the enzyme based on the coordination mode of two sulfate ions in our structure. Compared with Mycobacterium tuberculosis DapB in complex with its cofactor and inhibitor, we propose that the domain movement for active site constitution occurs when both cofactor and substrate bind to the enzyme.

• 한국음향학회지
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• 제38권3호
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• pp.290-301
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• 2019

본 논문에서 제안하는 알고리즘은 수중에서 협대역 지속파 능동 소나를 이용하여 표적 반향음을 탐지하는 문제를 다루고 있다. 능동 소나에서 표적 탐지를 위해 방사한 핑 신호는 주변의 많은 산란체에 의해 반사되는 신호를 만들어내며, 이를 잔향이라 한다. 본 논문에서 제안하는 알고리즘은 잔향 환경에서 낮은 도플러의 표적 반향음을 탐지하는 것을 목표로 한다. 제안하는 알고리즘은 빔공간 다채널 비음수 행렬 분해 기법을 기반으로 하여 방위, 주파수, 시간 기저를 추정하며, 특히 기저를 두 개의 기저집단 -잔향음 기저집단과 반향음 기저집단으로 나누어 독립적으로 추정한다. 제안하는 알고리즘의 성능을 평가하기 위하여 합성된 잔향 신호를 이용하여 시뮬레이션을 진행하였으며, 시뮬레이션 결과 제안하는 알고리즘이 기존의 알고리즘에 비해 향상된 성능을 보이는 것을 확인할 수 있었다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2008년도 하계종합학술대회
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• pp.83-84
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• 2008

It is efficient to use the time-domain beamforming to operate the various pulse with the different pulse length, frequency, bandwidth in active sonar system. In this paper, we propose a time-domain beamformer with the cumulative processing in the decomposed channel using the polynomial interpolation to solve the problem of the computational cost, high transmission data rate, and the lack of internal memory.

• 한국미생물·생명공학회지
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• 제24권1호
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• pp.67-71
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• 1996

The plasmid pK8 was constructed to verify the existence of an adenylate domain in peptide synthetase by using pGC12. 1.2 kb fragment, coding tyrocidine synthetase 1 (123 kDa) was deleted, and 79.6 kDa one was expressed in Escherichia coli XL1-blue. The truncated multienzyme activated phenylalanine and substrate analogues with comparable kinetics as the over expressed synthetase. ATP-[$^{32}P$]PPi exchange reaction was measured for the enzyme assay.

• Journal of Plant Biology
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• 제38권4호
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• pp.359-364
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• 1995

As the first step to elucidate the relationship between the structure and function of CTP:phosphocholine cytidylyltransferase (EC 2.7.7.15) in plants, the partial nucleotide sequence of soybean cytidylyltransferase cDNA was determined using a polymerase chain reaction (PCR). Degenerate oligonucleotide primers were synthesized from the conserved region revealed from the rat and yeast cytidylyltransferase DNA sequences. The catalytic domain region showed 78 and 76% homology with the rat and yeast amino acid sequences, respectivly. The hydropathy profile indicated that the C-terminal non-catalytic portion of the protein was very hydrophilic, and in the region between the catalytic domain and the C-terminal region, there was a large amphipathic $\alpha$-helical domain that was believed to bind the membrane surface in the active formation. There are 7 potential sites for phosphorylation by protein kinase C and 4 potential sites for phosphorylation by Ca2+/calmodulin kinase within the determined sequence.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2008년도 추계학술대회논문집
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• pp.612-613
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• 2008

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 1994년도 추계학술대회논문집; 한국종합전시장, 18 Nov. 1994
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• pp.331-336
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• 1994

본 논문에서는 음향궤환 문제해결을 위한 한 방법으로써, 넓은 주파수 영역에서 단일지향특성을 가질 수 있도록 주파수영역에서 음파분리를 하여, 하류측으로 전파되는 음파를 제어하는 새로운 방법을 제시한다.

• BMB Reports
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• 제40권4호
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• pp.539-546
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• 2007

The lysozymes encoded by bacteriophage T7 and K11 are both bifunctional enzymes sharing an extensive sequence homology (75%). The constructions of chimeric lysozymes were carried out by swapping the N-terminal and C-terminal domains between phage T7 and K11 lysozymes. This technique generated two chimeras, T7K11-lysozyme (N-terminal T7 domain and C-terminal K11 domain) and K11T7-lysozyme (N-terminal K11 domain and C-terminal T7 domain), which are both enzymatically active. The amidase activity of T7K11-lysozyme is comparable with the parental enzymes while K11T7-lysozyme exhibits an activity that is approximately 45% greater than the wild-type lysozymes. Moreover, these chimeric constructs have optimum pH of 7.2-7.4 similar to the parental lysozymes but exhibit greater thermal stabilities. On the other hand, the chimeras inhibit transcription comparable with the parental lysozymes depending on the source of their N-terminals. Taken together, our results indicated that domain swapping technique localizes the N-terminal region as the domain responsible for the transcription inhibition specificity of the wild type T7 and K11 lysozymes. Furthermore, we were able to develop a simple and rapid purification scheme in purifying both the wild-type and chimeric lysozymes.

• 한국수산과학회지
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• 제48권6호
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• pp.913-919
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• 2015

In vertebrates, the vitamin D receptor (VDR), a member of the nuclear receptor superfamily, binds the biologically active ligand $1{\alpha},25-(OH)_2$-vitamin $D_3$ (1,25 $D_3$). Nearly all vertebrates, including Agnatha, possess a VDR with high ligand selectivity for 1,25 $D_3$ and related metabolites. Although a putative ancestral VDR gene is present in the genome of the chordate invertebrate Ciona intestinalis, the functional characteristics of marine invertebrate VDR are still obscure. To elucidate the ascidian Halocynthia roretzi VDR (HrVDR), we cloned full-length HrVDR cDNA and investigated the transcriptional activity of HrVDR in HEK293 cells. HrVDR consists of 1,680 nucleotides (559 amino acids [aa]), including a short N-terminal region (A/B domain; 26 aa), DNA-binding domain (C domain; 72 aa), hinge region (D domain; 272 aa), and C-terminal ligand-binding domain (E domain; 161 aa). The amino acid sequence identity of HrVDR was greatest to that of C. intestinalis VDR (56%). In the luciferase reporter assays, the transcriptional activity of HrVDR was not significantly increased by 1,25 $D_3$, whereas the farnesoid X receptor agonist GW4064 increased the transactivation of HrVDR. These results suggest the presence of a novel ligand for and a distinct ligand-binding domain in ascidian VDR.