• 셀메드
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• 제6권4호
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• pp.23.1-23.6
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• 2016

With the rapid developments in nanotechnology, an increasing number of nanomaterials have been applied in various aspects of our lives. Recently, pharmaceutical nanotechnology with numerous advantages has growingly attracted the attention of many researchers. Zinc oxide nanoparticles (ZnO-NPs) are nanomaterials that are widely used in many fields including diagnostics, therapeutics, drug-delivery systems, electronics, cosmetics, sunscreens, coatings, ceramic products, paints, and food additives, due to their magnetic, catalytic, semiconducting, anti-cancer, anti-bacterial, anti-inflammatory, ultraviolet-protective, and binding properties. The present review focused on the recent research works concerning role of ZnO-NP on inflammation. Several studies have reported that ZnO-NP induces inflammatory reaction through the generation of reactive oxygen species by oxidative stress and production of inflammatory cytokines by activation of nuclear factor-${\kappa}B$ ($NF-{\kappa}B$). Meanwhile, other researchers reported that ZnO-NP exhibits an anti-inflammatory effect by inhibiting the up-regulation of inflammatory cytokines and the activation of $NF-{\kappa}B$, caspase-1, $I{\kappa}B$ $kinase{\beta}$, receptor interacting protein2, and extracellular signal-regulated kinase. Previous studies reported that size and shape of nanoparticles, surfactants used for nanoparticles protection, medium, and experimental conditions can also affect cellular signal pathway. This review indicated that the anti-inflammatory effectiveness of ZnO-NP was determined by the nanoparticle size as well as various experimental conditions. Therefore, the author suggests that pharmaceutical therapy with the ZnO-NP is one of the possible strategies to overcome the inflammatory reactions. However, further studies should be performed to maximize the anti-inflammatory effect of ZnO-NP to apply as a potential agent in biomedical applications.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2007년도 춘계종합학술대회
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• pp.491-494
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• 2007

최근 u-IT기술 발전과 디지털 컨버전스화에 따른 신규 부가가치 시장의 창출 및 이종 산업간 IT중심의 융 복합화가 빠르게 진행되고 있다. 이런 흐름속에서 주도적 역할을 할 것으로 기대되는 분야로 관심을 받고 있는 USN은 현재 산업의 성장사이클(Life Cycle) 상 기술개발 및 서비스 제공에 있어서 초기 단계로 판단된다. 하지만 향후 비약적 성장이 전망되는 블루오션 성격이 강하므로 원천기술 선점 및 관련 서비스의 우선 제공을 통해 향후 IT산업의 핵심 성장동력으로서 자리매김 할 수 있도록 하는 정책적 관심 및 지원이 절실히 필요하다. 이에 본고에서는 국내 USN 산업의 현황을 2006년 산업실태조사 결과를 바탕으로 USN공급기업의 매출액, 관련 기술, 전문인력, 사업 추진시 애로사항 요구사항 등에 관한 다각적 현황 분석을 통해 신산업육성 전략요소 및 모니터링요소에 대한 정책적 시사점을 도출하여 국내 USN산업의 조기활성화에 기여하고자 한다.

• 한국융합학회논문지
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• 제8권11호
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• pp.299-306
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• 2017

본 연구는 전수교육관의 대형화와 복합화 추세에 있어, 교육 외에도 방문객을 위한 차별화된 프로그램의 개발, 다양한 문화생활 지원을 위한 복합화의 방법, 그리고 지역주민들의 참여가 가능한 무형문화재 전수시설로서 공공성 증진을 위한 방안을 모색하고자 하는 것이다. 많은 중소도시의 경우 무형문화재전수시설은 전반적으로 복합화와 공공성을 수용하기 위해서는 공간이 미비한 상태여서, 전수교육관의 활성화를 위해 개선이 더욱 절실하다. 따라서 본 연구는 전수교육관의 활성화 방안을 모색하기 위한 기초연구로, 수도권 및 광역권 등의 대도시를 제외한 지역에 3곳의 전수교육관에 대한 방문조사를 통해 사용자 관점의 활성화 방안을 모색하기 위해 건립과 운영 실태를 파악하였다. 본 연구를 토대로 전수교육관이 본래의 기능과 더불어 공공성을 갖춘 복합문화공간으로 거듭나도록 기초연구자료로 활용되길 기대한다.

• International journal of advanced smart convergence
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• 제6권1호
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• pp.32-38
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• 2017

In Korea, the act for promoting use of the public data has been enforced since 2013. The Key Government 3.0 Strategy was established for providing the public data as an obligation of public authorities for users to use them free of charge. The aim of open public data and using them targeted by the government is creation of a solution for vitalizing the economy by using the public data. However, the reason for low usability and tangible outcomes despite unlimited provision of open government data is as follows. That is, it is essential to address the issue of difficult software technology required to access public data for using the data. However, with the open API method currently provided, the development procedure is not easy even for IT students or specialized software developers. Therefore, strategies for each step ideal for the level of developers are required to vitalize applications based on the public data and developed by ordinary users. Two strategic methods can be used for facilitating the use of public data and applications developed by ordinary users suggested in this paper on the basis of the public data portal organized by the Ministry of the Interior and provided to all ordinary users. That is, they are applications developed in the web browser environment and those developed in the PC environment. They allow ordinary users to develop and distribute applications based on the public data to contribute to enforcing the policy for facilitating the use of public data of the government just with basic training and basic knowledge from the training without using program coding knowledge of open API which requires the knowledge of development professionals.

• BMB Reports
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• 제53권3호
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• pp.142-147
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• 2020

Lipid accumulation in white adipose tissue is the key contributor to the obesity and orchestrates numerous metabolic health problems such as type 2 diabetes, hypertension, atherosclerosis, and cancer. Nonetheless, the prevention and treatment of obesity are still inadequate. Recently, scientists found that brown adipose tissue (BAT) in adult humans has functions that are diametrically opposite to those of white adipose tissue and that BAT holds promise for a new strategy to counteract obesity. In this study, we evaluated the potential of sinapic acid (SA) to promote the thermogenic program and lipolysis in BAT. SA treatment of brown adipocytes induced the expression of brown-adipocyte activation-related genes such as Ucp1, Pgc-1α, and Prdm16. Furthermore, structural analysis and western blot revealed that SA upregulates protein kinase A (PKA) phosphorylation with competitive inhibition by a pan-PKA inhibitor, H89. SA binds to the adenosine triphosphate (ATP) site on the PKA catalytic subunit where H89 binds specifically. PKA-cat-α1 gene-silencing experiments confirmed that SA activates the thermogenic program via a mechanism involving PKA and cyclic AMP response element-binding protein (CREB) signaling. Moreover, SA treatment promoted lipolysis via a PKA/p38-mediated pathway. Our findings may allow us to open a new avenue of strategies against obesity and need further investigation.

• The Korean Journal of Physiology and Pharmacology
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• 제24권4호
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• pp.299-310
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• 2020

Alzheimer's disease (AD) is a multi-faceted neurodegenerative disease. Thus, current therapeutic strategies require multitarget-drug combinations to treat or prevent the disease. At the present time, single drugs have proven to be inadequate in terms of addressing the multifactorial pathology of AD, and multitarget-directed drug design has not been successful. Based on these points of views, it is judged that combinatorial drug therapies that target several pathogenic factors may offer more attractive therapeutic options. Thus, we explored that the combination therapy with lower doses of cilostazol and aripiprazole with add-on donepezil (CAD) might have potential in the pathogenesis of AD. In the present study, we found the superior efficacies of donepezil add-on with combinatorial mixture of cilostazol plus aripiprazole in modulation of expression of AD-relevant genes: Aβ accumulation, GSK-3β, P300, acetylated tau, phosphorylated-tau levels, and activation of α-secretase/ADAM 10 through SIRT1 activation in the N2a Swe cells expressing human APP Swedish mutation (N2a Swe cells). We also assessed that CAD synergistically raised acetylcholine release and choline acetyltransferase (CHAT) expression that were declined by increased β-amyloid level in the activated N2a Swe cells. Consequently, CAD treatment synergistically increased neurite elongation and improved cell viability through activations of PI3K, BDNF, β-catenin and α7-nicotinic cholinergic receptors in neuronal cells in the presence of Aβ_1-42. This work endorses the possibility for efficient treatment of AD by supporting the synergistic therapeutic potential of donepezil add-on therapy in combination with lower doses of cilostazol and aripiprazole.

• 한국전문물리치료학회지
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• 제25권3호
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• pp.51-59
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• 2018

Background: Despite muscle latency times and patterns were used as broad examination tools to diagnose disease and recovery, previous studies have not compared the dominant arm to the non-dominant arm in muscle latency time and muscle recruitment patterns during reaching and reach-to-grasp movements. Objects: The present study aimed to investigate dominant and non-dominant hand differences in muscle latency time and recruitment pattern during reaching and reach-to-grasp movements. In addition, by manipulating the speed of movement, we examined the effect of movement speed on neuromuscular control of both right and left hands. Methods: A total of 28 right-handed (measured by Edinburgh Handedness Inventory) healthy subjects were recruited. We recorded surface electromyography muscle latency time and muscle recruitment patterns of four upper extremity muscles (i.e., anterior deltoid, triceps brachii, flexor digitorum superficialis, and extensor digitorum) from each left and right arm. Mixed-effect linear regression was used to detect differences between hands, reaching and reach-to-grasp, and the fast and preferred speed conditions. Results: There were no significant differences in muscle latency time between dominant and non-dominant hands or reaching and reach-to-grasp tasks (p>.05). However, there was a significantly longer muscle latency time in the preferred speed condition than the fast speed condition on both reaching and reach-to-grasp tasks (p<.05). Conclusion: These findings showed similar muscle latency time and muscle activation patterns with respect to movement speeds and tasks. Our findings hope to provide normative muscle physiology data for both right and left hands, thus aiding the understanding of the abnormal movements from patients and to develop appropriate rehabilitation strategies specific to dominant and non-dominant hands.

• Journal of Ginseng Research
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• 제42권3호
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• pp.379-388
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• 2018

Background: Ginsenosides are the main ingredients of Korean Red Ginseng. They have extensively been studied for their beneficial value in neurodegenerative diseases such as Parkinson's disease (PD). However, the multitarget effects of Korean Red Ginseng extract (KRGE) with various components are unclear. Methods: We investigated the multitarget activities of KRGE on neurological dysfunction and neurotoxicity in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of PD. KRGE (37.5 mg/ kg/day, 75 mg/kg/day, or 150 mg/kg/day, per os (p.o.)) was given daily before or after MPTP intoxication. Results: Pretreatment with 150 mg/kg/day KRGE produced the greatest positive effect on motor dysfunction as assessed using rotarod, pole, and nesting tests, and on the survival rate. KRGE displayed a wide therapeutic time window. These effects were related to reductions in the loss of tyrosine hydroxylase-immunoreactive dopaminergic neurons, apoptosis, microglial activation, and activation of inflammatory factors in the substantia nigra pars compacta and/or striatum after MPTP intoxication. In addition, pretreatment with KRGE activated the nuclear factor erythroid 2-related factor 2 pathways and inhibited phosphorylation of the mitogen-activated protein kinases and nuclear factor-kappa B signaling pathways, as well as blocked the alteration of blood-brain barrier integrity. Conclusion: These results suggest that KRGE may effectively reduce MPTP-induced neurotoxicity with a wide therapeutic time window through multitarget effects including antiapoptosis, antiinflammation, antioxidant, and maintenance of blood-brain barrier integrity. KRGE has potential as a multitarget drug or functional food for safe preventive and therapeutic strategies for PD.

• Toxicological Research
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• 제30권3호
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• pp.149-157
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• 2014

Smoking is one of the most serious but preventable causes of cardiovascular disease (CVD). Key aspects of pathological process associated with smoking include endothelial dysfunction, a prothrombotic state, inflammation, altered lipid metabolism, and hypoxia. Multiple molecular events are involved in smoking-induced CVD. However, the dysregulations of reactive oxygen species (ROS) generation and metabolism mainly contribute to the development of diverse CVDs, and NADPH oxidase (NOX) has been established as a source of ROS responsible for the pathogenesis of CVD. NOX activation and resultant ROS production by cigarette smoke (CS) treatment have been widely observed in isolated blood vessels and cultured vascular cells, including endothelial and smooth muscle cells. NOX-mediated oxidative stress has also been demonstrated in animal studies. Of the various NOX isoforms, NOX2 has been reported to mediate ROS generation by CS, but other isoforms were not tested thoroughly. Of the many CS constituents, nicotine, methyl vinyl ketone, and ${\alpha}$,${\beta}$-unsaturated aldehydes, such as, acrolein and crotonaldehyde, appear to be primarily responsible for NOX-mediated cytotoxicity, but additional validation will be needed. Human epidemiological studies have reported relationships between polymorphisms in the CYBA gene encoding p22phox, a catalytic subunit of NOX and susceptibility to smoking-related CVDs. In particular, G allele carriers of A640G and $-930^{A/G}$ polymorphisms were found to be vulnerable to smoking-induced cardiovascular toxicity, but results for C242T studies are conflicting. On the whole, evidence implicates the etiological role of NOX in smoking-induced CVD, but the clinical relevance of NOX activation by smoking and its contribution to CVD require further validation in human studies. A detailed understanding of the role of NOX would be helpful to assess the risk of smoking to human health, to define high-risk subgroups, and to develop strategies to prevent or treat smoking-induced CVD.

• 디지털융복합연구
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• 제17권2호
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• pp.101-111
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• 2019

냉동 냉장창고의 집적단지인 '콜드체인 클러스터' 구축이 물류업계의 현안으로 급부상하고 있다. 인천항만공사는 한국가스공사와 협력하여 송도 LNG 인수기지에서 발생하는 냉열 에너지를 이용한 냉동 냉장 클러스터를 구축하기 위한 사업을 진행 중에 있다. 본 연구에서는 CFPR 방법론을 활용하여 냉동 냉장 클러스터 활성화 방안을 제시하는 것을 연구의 목적으로 한다. 분석결과, 상위요인에서는 안정성과 수익성 요인이 0.281로 가장 중요시 되는 요인으로 파악되었다. 세부요인으로는 지속가능한 물동량 확보가 가장 높게 나타났고, 임대료 수준, LNG 냉열 활용기술의 지속 가능성과 일반 냉동 냉장창고와의 경쟁, 시설 중복투자 배제 등의 순으로 중요도가 평가 되었다. 향후 연구에서는 활성화 요인을 활용하여 국내 지역별 냉동냉장클러스터를 평가할 필요가 있다.