• Title/Summary/Keyword: activated sludge

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PCR-DGGE as a Supplemental Method Verifying Dominance of Culturable Microorganisms from Activated Sludge

  • Zhou, Sheng;Wei, Chaohai;Ke, Lin;Wu, Haizhen
    • Journal of Microbiology and Biotechnology
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    • v.20 no.11
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    • pp.1592-1596
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    • 2010
  • To verify the dominance of microorganisms in wastewater biological treatment, PCR-DGGE (denaturing gradient gel electrophoresis) was performed as a supplementary support method for screening of the dominant microorganisms from activated sludge. Results suggest that the dominant microorganisms in activated sludge are primarily responsible for strengthening its effectiveness as a biological treatment system, followed by the non-main dominant microorganisms, whereas the non-dominant microorganisms showed no effects. The degree of microbial abundance present on the profile of PCR-DGGE was in line with the treatment efficiency of augmented activated sludge with isolated cultures, suggesting that PCR-DGGE can be used as an effective supplementary method for verifying culturable dominant microorganisms in activated sludge of coking wastewater.

Bioremediation of Phenolic Compounds Having Endocrine-disrupting Activity Using Ozone Oxidation and Activated Sludge Treatment

  • Nakamura, Yoshitoshi;Daidai, Masakazu;Kobayashi, Fumihisa
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.3
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    • pp.151-155
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    • 2004
  • The bioremediation of water system contaminated with phenolic compounds having endocrine-disrupting activity, i.e. 2,4-dichlorophenol, 2,4-dichlorophenoxy acetic acid (2,4-D), and 2,4,5-trichlorophenoxy acetic acid (2,4,5-T), was investigated by using ozone oxidation and activated sludge treatment. Ozone oxidation (ozonation time: 30 min) followed by activated sludge treatment (incubation time: 5 days) was an efficient treatment method for the conversion of phenolic compounds in water into carbon dioxide and decreased the value of total organic carbon (TOC) up to about 10% of initial value. Furthermore, 2,4-D was dissolved in water containing salt, i.e. artificial seawater (ASW), and this water was used as model coastal water contaminated with phenolic compounds. The activated sludge treatment (incubation time: 5 days) could consume significantly organic acids produced from 2,4-D in the model costal water by the ozone oxidation (ozonation time: 30min) and decrease the value of TOC up to about 35% of initial value.

Removal of Nitrogen and Phosphorus by Activated Sludge Process Combined with Aluminum Corrosion (알루미늄 부식을 적용한 활성슬러지법에서 질소 및 인 제거)

  • Choi Hyung Il;Cheong Kyung Hoon;Park Sang Il;Paik Ke Jin
    • Journal of Environmental Health Sciences
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    • v.31 no.2 s.83
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    • pp.172-178
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    • 2005
  • A laboratory experiment was performed to investigate simultaneous removal of phosphorus and nitrogen from raw sewage by intermittently aerated activated sludge process packed with aluminum and silver plate. Two continuous experimental process, i.e. an intermittently aerated activated sludge process, and an intermittently aerated activated sludge process with an aluminum and silver plate packed into the reactor were compared. The pitting corrosion of aluminum does not affect the performance of the biological treatment. The amounts of Al eluted from aluminum plate 17 mg to 60.6 mg in this experimental conditions, and Al/P mole ratio were from 3.31 to 11.25. The total nitrogen removal efficiency in Run E were $60.6\%$ at the HRT of 12 hours. The effluent $PO_4-P$ concentration as low as $1.0\;mg/\iota$ could have been obtained during the continuous experiment in Run E at HRT of 12 hours.

Soybean Wastewater Treatment by Activated Sludge Process (고농도 대두가공폐수의 처리를 위한 개선 활성슬러지법)

  • Cho, Kwon-Ik;Lee, Jeoung-Su;Lee, Tae-Kyoo;Kim, Jong-Hwa
    • Applied Biological Chemistry
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    • v.45 no.1
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    • pp.25-29
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    • 2002
  • The kernel of wastewater treatment by activated sludge is elimination of organic substances and maintenance of well-flocculated sludge sedimentation. By the conventional activated sludge treatment, the optimum F/M ratio of soybean wastewater treatment was 0.24 (kg-BOD/kg-MLVSS day) and sludge bulking was generated at 0.48 (kg-BOD/kg-MLVSS day). To improve the treatment capacity and operation quality in higher loading of soybean wastewater, influent pH was constantly controlled by 9.0 using NaOH as a coagulant agent. In this process, higher loading up to 2.88 (kg-BOD/kg-MLVSS day) was possible and SVI was maintained under 150 without bulking. This was equivalent to 7.2 times higher than maximum permissible load of the conventional activated sludge process.

Fuzzy algorithm of Automatic control for dissolved oxygen in Activated sludge aeration tank (활성슬러지 폐수처리장 폭기조 DO제어를 위한 퍼지 제어 알고리즘 연구)

  • 손건태;김성덕;고주형
    • Journal of Environmental Science International
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    • v.8 no.4
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    • pp.533-538
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    • 1999
  • Fuzzy algorithm of automatic control for dissolved oxygen(DO) concentration in the aeration tank of an activated sludge process is proposed. Among variables repirometry and air flowrate are selected as significant input factors and the relationship with DO is estimated using a multiple regression model. The DO concentration and the amount of repirometry are fuzzified and the fuzzy rule base are determined. Using the fuzzy algorithm, the change of amount of air flowrate are determined and the change of amount of DO is derived.

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Thickening Characteristics of Activated Sludge by Air Flotation Process (공기부상법을 이용한 활성슬러지의 부상분리 특성)

  • Park, Chanhyuk;Hong, Seok-won;Maeng, Juwon;Lee, Sanghyup;Choi, Yong-su;Moon, Seong-yong
    • Journal of Korean Society of Water and Wastewater
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    • v.20 no.5
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    • pp.747-753
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    • 2006
  • Air flotation is a solid-liquid separation process that utilizes up-flow microbubbles to thicken activated sludge and enhance clarification efficiency. Continuous air flotation experiments were performed to investigate the effect of operation parameters such as initial MLSS (mixed liquor suspended solid) concentration, air pressure, surface loading rate, air to solid (A/S) ratio, and flotation time on thickening efficiency. An initial activated sludge concentration ranged from 3,000 to 12,000mgSS/L and thickened sludge concentration varied from 6,400 to 28,100mgSS/L. The result showed that the thickening efficiency was mainly dependent on surface loading rate, A/S ratio, and flotation time. The pressure did not affect the thickening efficiency when it kept in the range of 1.6 to 1.8 bar. Experimental results showed that the thickening efficiency of activated sludge was increased only when the feed sludge concentration exceeded 5,000mgSS/L and the thickened concentration was over 20,000mgSS/L. At this time, SS concentration in the clarified liquid was ranged from 5 to 10mg/L.

Treatment Characteristics of Paper-mill Wastewater Using Pure Oxygen Activated Sludge Process (순산소 활성오니 공정을 이용한 제지폐수의 처리특성)

  • Kim, Sung Soon;Chung, Tai Hak
    • Journal of Korean Society of Water and Wastewater
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    • v.13 no.4
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    • pp.27-34
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    • 1999
  • An experimental study on improvement of the paper-mill wastewater treatment using the pure oxygen activated sludge process was conducted. The effects of hydraulic retention time(HRT) and BOD loading on organic removal efficiency were investigated. The BOD removal efficiencies were above 90% under all examined HRTs except for HRT of 3 hours. The increase of HRT from 3 hours to 6 hours, and to 12 hours significantly improved BOD and COD removal efficiencies, respectively. However, additional increase of HRT did not affect organic removal efficiency. F/M ratio change at fixed HRT did not affect organic removal efficiency. However, F/M ratio investigated in this study(0.11~1.98kgBOD/kgMLVSS/day) was 5 times greater in maximum than that of conventional activated sludge process, which implies that pure oxygen activated sludge process can treat wastewater with high organic strength. Under the same HRT, the volumetric BOD loading change cause no effect on organic removal efficiency also.

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Biological Toxicity Evaluation of Heavy Metal using Oxygen Uptake Rate on Activated Sludge Process (호흡율 측정에 의한 활성슬러지의 중금속 생물독성평가)

  • Ahn, Woo Jung;Baek, Seung-Kyu
    • Journal of Korean Society of Water and Wastewater
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    • v.21 no.3
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    • pp.279-285
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    • 2007
  • Toxicity evaluations using oxygen uptake rate (OUR) on activated sludge process were investigated. On toxicity evaluations of heavy metals, all toxicants in experiments decreased OUR with respect to the concentrations of the toxicants, while high toxicities with cyanide and mercury were observed respectively. On toxicity evaluations of composite heavy metals, composite toxicities had similar results as to the total sum of each toxicants that presents accurate toxicity evaluation using OUR. From these results, it is concluded that activated sludge is effective indicator for toxicity evaluation on wastewater of biological treatment plants.

Uptake of Wastewater Organic Matter to Activated Sludge

  • Nam, Se-Yong;Kim, In-Bae
    • Journal of Environmental Health Sciences
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    • v.33 no.6
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    • pp.493-496
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    • 2007
  • The influences of contact time and ratio of food to microorganism (F/M) on uptake of wastewater organic matter in a short contact process were investigated using three activated sludge batch reactors fed with synthetic wastewater, sewage and livestock wastewater. About 64% of influent soluble chemical oxygen demand (SCOD) in the synthetic wastewater and 61% of SCOD in the sewage and 43% of SCOD in the diluted livestock wastewater were adsorbed into the activated sludge within 30 min. The specific mass of organic matter uptaken in the synthetic wastewater was 55 mg SCOD/g mixed liquor suspended solids (MLSS). In the same manner, 20 and 14 mg SCOD/g MLSS were calculated as the values in the sewage and livestock wastewater, respectively.

활성슬러지 고정화 비드를 이용한 페놀 분해에 관한 연구

  • Kim, Seon-Il
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.431-434
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    • 2002
  • Effect of various factors on the phenol degradation by activated sludge immobilized with the photocrosslinked resin were investigated. The optimum pH on the degradation of phenol in both free and immobilized activated sludge was 7. A higher rate of phenol degradation was observed when a bead size, vas smaller. The phenol degradation in the free activated sludge was inhibited at the 3000 mg/L of phenol, while that in the immobilized activated sludge was maintained at the same concentration for 28 hrs without an inhibition.

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