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http://dx.doi.org/10.4014/jmb.0908.08012

PCR-DGGE as a Supplemental Method Verifying Dominance of Culturable Microorganisms from Activated Sludge  

Zhou, Sheng (College of Environmental Science and Engineering South China University of Technology, The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education)
Wei, Chaohai (College of Environmental Science and Engineering South China University of Technology, The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education)
Ke, Lin (College of Environmental Science and Engineering South China University of Technology, The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education)
Wu, Haizhen (School of Bioscience and Bioengineering, South China University of Technology)
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.11, 2010 , pp. 1592-1596 More about this Journal
Abstract
To verify the dominance of microorganisms in wastewater biological treatment, PCR-DGGE (denaturing gradient gel electrophoresis) was performed as a supplementary support method for screening of the dominant microorganisms from activated sludge. Results suggest that the dominant microorganisms in activated sludge are primarily responsible for strengthening its effectiveness as a biological treatment system, followed by the non-main dominant microorganisms, whereas the non-dominant microorganisms showed no effects. The degree of microbial abundance present on the profile of PCR-DGGE was in line with the treatment efficiency of augmented activated sludge with isolated cultures, suggesting that PCR-DGGE can be used as an effective supplementary method for verifying culturable dominant microorganisms in activated sludge of coking wastewater.
Keywords
Biological treatment; dominant microorganisms; non-dominant microorganisms; PCR-DGGE; non-main dominant microorganisms;
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