• 대한한방내과학회지
• /
• 제16권1호
• /
• pp.181-196
• /
• 1995

The present experiments were designed to investigate the effects of BangPoongSan on the cardiovascular system in the experimental Animals. And thus the change of blood pressure, auricular blood flow, artery contraction, death rate, platelet aggregation repression, plasma coagulation factor activity, plasma antithrombin activity, whole blood viscosity and plasma viscosity were studied. The result were summarized as the followings: 1. BangPoongSan dropped the blood pressure in the spontaneous hypertensive rat. 2. The drug increased the auricular blood flow in rabbit. 3. The drug relaxed the artery contraction by pretreated norepinephrine in white rat. 4. The drug inhibited the death rate of mouse which was led to thromboembolism by serotonin and collagen. 5. The drug inhibited the platelet aggregation in rat. 6. The drug prolonged the prothrombin time and activated partial thromboplastin time on the test of plasma coagulation factor activity in rat, but was not valuable. 7. The drug presented the antithrombin activity in rat. 8. The drug reduced the whole blood viscosity and plasma viscosity in rat, but the latter was not valuable. According to the results, Bangpoongsan increased the blood flow and dropped the blood pressure by dilatation of blood vessel smooth muscle. And the drug presented the antithrombin acivity, inhibited the platelet aggregation and reduced blood viscosity. Therefore these effects are assumed to improve the cardiovascular circulation disorder and prevent thrombosis.

• 한국마린엔지니어링학회:학술대회논문집
• /
• 한국마린엔지니어링학회 2006년도 전기학술대회논문집
• /
• pp.273-274
• /
• 2006

The sea is origin of all lift, and 90% of the all living organisms are in the sea. The biosynthesis is very different. Many organisms are kept on a lower or developed to another evolutionary level than on shore. Our society is increasing demand and need for marine food and this food has to product at onshore or offshore fish farming sites. Ocean fish farms have a special operation properties such as a good quality water, net cage, sheltered locations and feeding system. The farming site is controlled and monitored for fish welfare as ammonia($NH_3$), temperature, the speed of a running fluid. Specially, the fish farm is seriously influenced by ammonia. In this paper, $NH_3$ monitoring system for ocean fish farm is researched for the suitable fish farming sites, and test equipment is designed for achieving practical data. The equipment wit monitoring algorithm is expected to the useful system for ocean fish farm.

• The Plant Pathology Journal
• /
• 제15권6호
• /
• pp.313-318
• /
• 1999

Cloning of the 5'untranslated region (5' UTR) and Nterminus of the glycoprotein precursor (G2G1) open reading frame of tomato spotted wilt virus has been problematic, possibly because of the toxicity of a signal peptide at the beginning of th G2G1 protein precursor. The toxicity of the signal peptide to bacterial growth and the reason for the expression of the peptide gene in Escherichia coli were investigated by cloning the 5' UTR and the signal peptide sequence separately. Cells transformed with the plasmid containing both the first 30 amino acids of the glycoprotein and the 5' UTR showed a severe growth inhibition whereas transformants harboring either the plasmid with the signal sequence or the 5'UTR alone did not show any ingibition. An E. coli promoter-like sequence was found in the 5'UTR and tis promoter acivity was confirmed with a promoter-less GUS gene cloned downstream of the 5'UTR. In the cloning of the Tomato spotted wilt virus (TSWV) glycoprotein G2G1 open reading frame all the recovered plasmids contained stop codons in the signal sequence region. However, clones containing no stop codon were recovered when the signal sequence and the 5'UTR were cloned separately.

• 한국식품영양학회지
• /
• 제31권5호
• /
• pp.720-728
• /
• 2018

The purpose of this study is to identify awareness of hypertension prevalence among Korean youth and related causes. Data from the Korean National Health and Nutritional Examination Survey, conducted in 2016 by the Korea Centers for Disease Control and Prevention, were used to apply weights for complex sample analysis. A total of 1,075 data age 19~35 Korean youth were used for analysis. The analysis demonstrated that 25.7% of age 19~35 Korean youth were in the prehypertension stage and 6.1% were in the hypertension stage. Those aware of hypertension was 0.3%. This revelation necessitates establishment of preventive measures by way of physical examination among youth to ensure no youth are left untreated in terms of health management. This study indicated that diabetes affects hypertension significantly among males (p<0.0001), with low income (p=0.034) and obesity (p<0.0001). Consequently, they should be managed as high-risk, and it is imperative to educate them about changes in lifestyle such as walking or less dining-out, rather than engaging in specific sports activities or nutrient intake.

• 한국잠사학회:학술대회논문집
• /
• 한국잠사학회 2003년도 제46회 춘계 학술연구 발표회
• /
• pp.45-45
• /
• 2003

Protein disulfide isomerase (PDO) is an essential protein which is localized to the endoplasmic reticulum (ER) of eukaryotic cells. It catalyses the formation and isomerization of disulfide bonds during the folding of secretory proteins. We have isolarted a cDNA encoding PDI from Bombyx mori (bPDI), in which an open reading frame of 494 mino acid (55.6kDa) is shown. (omitted)

• Mycobiology
• /
• 제33권2호
• /
• pp.90-96
• /
• 2005

Sixty two out of the sixty four species of fungal isolates tested could produce both $exo-{\beta}1,4-gluconase\;(C_1)$ and $endo-{\beta}1,4-gluconase\;(C_x)$ on pure cellulose and rice straw as carbon source in Czapek's medium. Fifty-eight and fifteen species were able to grow at $25^{\circ}C$ and at $45^{\circ}C$, respectively. Eleven species could grow at both $25^{\circ}C$ and $45^{\circ}C$ while, four species appeared only at $45^{\circ}C$. The most cellulolytic species at $25^{\circ}C$ was Trichoderma koningii producing 1.164 $C_1$ (mg glucose/1 ml culture filtrate/1 hr) and 2.690 $C_x$ on pure cellulose, and 0.889 $C_1$, and 1.810 $C_x$ on rice straw, respectively. At $45^{\circ}C$, the most active thermotolerant species were Aspergillus terreus, followed by A. fumigatus. Talaromyces thermophilus was the highest active thermophilic species followed by Malbranchea sulfurea. Most of these species were also active in fermentation of rice straw at 25 and $45^{\circ}C$ (P<0.05). The most active ones were T. koningii, A. ochraceus and A. terreus, which produced 201.5, 193.1 and 188.1 mg crude protein/g dry straw, respectively.

• 생명과학회지
• /
• 제8권2호
• /
• pp.145-157
• /
• 1998

We isolated 100 Vobrio sp. from marine products and sea from July to September, 1997. We attemped on purification of hemolysin produced by Vibrio sp. The growth, hemolysin production patterns by the 100 strains of Vibrio sp. showed identical, in general. V. unlnificus ys-1 produced hemolysis as the higtest titer. The optimal culture conditions for the hemolysin production by the V. vunificus ys-1 are followings; 1. Hemolysin production was optimal dering the late exponetial phage. 2. Maximal growth, hemolysin production were in heart infusion broth. 3. Maximal yields of hemolysin was obtained when the heart infusion broth had an intial pH of 8.0, 3$0^{\circ}C$, 3% NaCL. Hemolysin was purified from culture filtrate of the strain by ammonium sulfate recipitation, ion exchange and hydrophobic interaction chromatography. The results were as follows; 1. Hemogeneity of the purified hemolysin was demonstrated by revealing single band on SDS-PAGE. The molecular weight of purified hemolysin was 45KDa. 2. The absorbance rattern in ultraviolet wsa typical of those seen with most proteinb with 280nm. 3. Purified hemolysin was atable at 5$0^{\circ}C$ but 7$0^{\circ}C$ of the acivity was lost by heating for 30 min at 6$0^{\circ}C$/ Optimal temperature of purified hemolysin was 35$^{\circ}C$. 4. Purified hemolysin was stable at the pH range of 6~9, but in the less the pH5.0. above the pH 9.0, the hemolysin activity was lost completely.

• 전기화학회지
• /
• 제17권3호
• /
• pp.201-208
• /
• 2014

본 연구에서는 산화그래핀과 카본블랙의 혼합담체를 이용하여 내구성이 향상된 백금촉매를 폴리올법으로 제조하였다. 삼전극 순환전압전류법을 이용한 전기화학성능 측정결과 적절한 비율로 조절된 혼합담지체에 백금을 담지시켰을 경우 초기 성능 감소없이 장기내구성이 향상되는 것으로 나타났다. 또한 회전원판전극을 이용하여 산소환원반응을 수행한 결과 혼합담체에 담지된 백금촉매가 카본블랙 단일담체에 담지된 백금촉매보다 우수한 고유활성값을 나타내었다.

• 한국식품영양과학회지
• /
• 제18권1호
• /
• pp.40-46
• /
• 1989

보리는 $0.94{\sim}1.17%$의 phytic acid를 함유하였다. 쌀보리의 경우 70% 수율로, 겉보리의 경우 60%로 도정한 결과 $71{\sim}83%$의 phytic acid가 감소되었다. 쌀보리를 가열, 증자처리하였을 때, phytic acid 는 감소되지 않았으나 초음파로 처리한 결과 57%가 감소하였다. 맥주맥의 발아시 phytase 활성도는 발아 4일후에 최대치를 나타냈고 phytic acid의 함량은 감소하여 발아 5 일후에는 24%정도 감소하였다. 유안염석과 gel 여과 및 DEAE-cellulose chromatography등으로 정제된 phytase는 작용 최적pH가 5.0이였으며 $40^{\circ}C$에서 최대활성도를 보였다.

• 대한한방내과학회지
• /
• 제22권2호
• /
• pp.215-222
• /
• 2001

Against the mutagen MNNG and NOPD with SOS chromotest, the antigenotoxic activity of MeOH-soluble extract was much more effective than that of the water-soluble one. When the extract was added to the certain concentration, the antigenotoxic acivity was enhanced. Against the mutagen NOPD with Ames test, the antimutagenic activity of MeOH-soluble extract was better than that of the water-soluble one. The 60.4% of the inhibition ratio for the revertant colony-forming unit was shown at 5 mg of MeOH-soluble extract per plate. Antimutagenecity test with SOS chromotest and Ames test were performed using water-soluble and MeOH-soluble extracts from of Gleditsia sinensis. The antioxidant activity of MeOH-soluble extract with the NBT method was higher than that of the water-soluble one.