• Title/Summary/Keyword: acidic compounds

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Production of Carrot Pomace Fortified with Mucilage, Fibrinolytic Enzyme and Probiotics by Solid-state Fermentation Using the Mixed Culture of Bacillus subtilis and Leuconostoc mesenteroides

  • Jung, Hye-Won;Lee, Sam-Pin
    • Preventive Nutrition and Food Science
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    • v.14 no.4
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    • pp.335-342
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    • 2009
  • Bioactive compounds were produced from carrot pomace by solid-state fermentation using Bacillus subtilis HA and Leuconostoc mesenteroides. The carrot pomace (CP) fermented by B. subtilis HA with 3% monosodium glutamate (MSG) showed higher production of various bioactive compounds, with 1.64 Pa·sn of consistency, 2.31% of mucilage content, 16.95 unit/g of fibrinolytic enzyme activity, 35.3 unit/g of proteolytic activity and 37.5 mg% of tyrosine content. The mucilage production was greatly dependent upon the concentration of MSG added. Most MSG added in CP was converted into mucilage (2.3%) including 0.83% poly-$gamma$-glutamic acid (PGA) with 1,505 kDa of molecular weight. The CP fermented secondly by Leuc. mesenteroides showed acidic pH and lower consistency. However, the fibrinolytic and proteolytic activities were increased. The secondly fermented CP showed the viable cell counts with $2.5{\time}108$ CFU/g of B. subtilis HA and $3.7{\time}109$ CFU/g of Leuc. mesenteroides, respectively. The freeze-dried fermented CP showed 2.88 Pa·sn of consistency, 24% of mucilage content and 104.9 unit/g of fibrinolytic enzyme activity, respectively. Also, the powder of fermented CP indicated viable cell counts of $8.0{\time}107$ CFU/g of B. subtilis and $4.0{\time}108$ CFU/g of Leuc. mesenteroides. Therefore, the fermented CP that was fortified with dietary fibers, fibrinolytic enzyme and probiotics could be utilized as valuable ingredients of functional foods in food or cosmetic industries.

Antimicrobial Activities of Extracts from Several Native and Exotic Plants in Korea (수 종의 한국자생식물과 귀화식물 추출액이 토양미생물에 미치는 항균활성)

  • Kim, Yong-Ok;Lee, Eun-Ju;Lee, Ho-Joon
    • The Korean Journal of Ecology
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    • v.23 no.5
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    • pp.353-357
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    • 2000
  • The soil pH favored by several native plants in Korea ranges 5.33∼7.20, while a more acidic range of pH 3.95∼6.10 is acceptable to exotic plants. Ethanol extracts of native and exotic plants in Korea were investigated for antimicrobial activity against Bacillus sphiaericus 2362, Bacillus thuringiensis var. subtilis and Bacillus thuringiensis var. cereus and Actinomycetes. Higher antimicrobial activity was observed from the extract of exotic plants than those of native plants. The ethanol extract of Ambrosia artemisiifolia var. elatior was observed to have the highest antimicrobial activity against 4 species of soil microbes. Especially, antimicrobial activity of Ambrosia artemisiifolia var. elatior showed the largest clear zone of 48mm in Actinomycetes. Larger clear zone was formed in the order of caffeic acid, benzoic acid and ρ -coumaric acid among the nine chemical compounds. Accordingly, the antimicrobial activity of Ambrosia artemisiifolia var elatior against Actinomycetes was found to be due to the synergetic effect of chemical compounds.

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The Effect of Glyceride Modified by Fatty Acid on Mechanical Properties of Silica filled Rubber Compounds (지방산으로 개질된 글리세라이드가 실리카 충진 배합고무의 가황과 기계적 물성에 미치는 영향)

  • Kim, Dong-Wook;Kim, Chang-Hwan;Jung, Ho-Kyun;Kang, Yong-Gu
    • Elastomers and Composites
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    • v.48 no.2
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    • pp.114-124
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    • 2013
  • To study the effects of structural difference and fatty acid chain length of glyceride, new dispersion agents having various glyceride structures such as mono-, di-, and tri-, were prepared using glycerol extracted from palm oil and fatty acid having various chain length ranges from 12 to 18. These dispersion agents were mixed with the rubber compounds and compared with conventional metal salt dispersion agents. Glyceride dispersion agent provided remarkable improvement in silica dispersion, compared to metal salt fatty acidic one, even though the viscosity of mixtures was relatively high due to low lubricating effect, and this was approved by mechanical properties, wear properties, and Payne effect. Also, the longer in chain length of fatty acid and the smaller in numbers of fatty acid, the dispersity of silica was improved.

Isolation and Identification of Two Psoralen Derivatives as Antioxidative Compounds from the Aerial Parts of Angelica keiskei (신선초에 함유된 항산화활성물질 Psoralen 유도체들의 단리 및 동정)

  • Kim, So-Joong;Cho, Jeong-Yong;Wee, Ji-Hyang;Jang, Mi-Young;Rim, Yo-Sup;Kim, Cheol;Shin, Soo-Cheol;Moon, Jae-Hak;Park, Keun-Hyung
    • Korean Journal of Food Science and Technology
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    • v.37 no.4
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    • pp.656-659
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    • 2005
  • Hot water extracts of Angelica keiskei aerial parts were solvent-fractionated with ethyl acetate (EtOAc) and buffers (5% $NaHCO_3$, pH 8.0; 1.0 N HCl, pH 3.0) to obtain EtOAc-soluble acidic and neutral fractions. EtOAc-soluble neutral fraction was purified by Sephadex LH-20 column chromatography and reverse phase HPLC. Assay for purification of antioxidative compounds was performed by spraying DPPH solution on thin layer chromatography. Two isolated substances were identified as pseudoisopsoralen and 8-methoxypsoralen(xanthotoxin) by FAB-MS and NMR analyses.

Aging Property Studies on Rubber Gasket for Polymer Electrolyte Membrane Fuel Cell Stack (고분자 전해질 연료전지 스택용 고무 개스킷의 노화특성 연구)

  • Kang, Dong-gug;Hur, Byung-ki;Lee, Dong-won;Seo, Kwan-ho
    • Applied Chemistry for Engineering
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    • v.22 no.2
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    • pp.149-154
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    • 2011
  • In order to explore properties of various rubber compounds after thermal aging under the condition similar to the operating environment of a fuel cell-stack, heat resistance and compression set of those compounds were investigated for a long term operation in $H_2SO_4$, $H_2O$, and LLC (ethylene glycol : $H_2O=50:50$) solution. It was assumed that aging Acrylonitrile butadiene rubber (NBR) and Elthylene Propylene diene rubber (EDPM) compound in the solution resulted in discoloration as time passed. It was also found that hydrolysis was developed on the Silicone rubber (VMQ) compound intentionally aged under acidic condition by means of TGA, SEM, and EDS analysis.

Effects of Alkali Treatment of Soybean on the Qualty of Soybean Milk (대두(大豆)의 Alkali 처리가 두유의 품질에 미치는 영향)

  • Oh, Joon-Sei;Lee, Gyu-Hee;Lee, Won-Yong;Lee, Ka-Sun;Oh, Man-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.17 no.2
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    • pp.85-94
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    • 1988
  • This experiment was carried out to obtain the basic data for removal the astringency and off-flavor in soybean milk, by means of soaking of soybean in NaOH and $NaHCO_3$ solutions. The changes of phenolic compounds in soybean during soaking were investigated with HPLC and also the changes of flavor and sensority of soybean milk, prepared from soaked soybean were studied. Phenolic compounds of soybean were identified as chlorogenic, p-hydroxybenzoic, p-coumaric, ferulic and gentisic acid and, chlorogenic acid content was greater than the others. The chlorogenic acid of soybean was mainly neutral type and the other compounds were almost acidic type. Up to 85% of the chlorogenic acid was removed by soaking of soybean in 0.1% of NaOH solution for 8 hrs. Phenolic compounds of soybean was almost removed by soaking in 0.1% of NaOH solution at $90^{\circ}C$ for 1 hr. Chemical composition of soybean milks prepared from soaking of soybean in water, 0.1% NaOH and 0.5% $NaHCO_3$ solution were similar. Hexanol content of beany flavor in soybean milk was increased by soaking of soybean in NaOH solution, where as hexanal, propanal, pentanal contents were removed up to 60%. Color of soybean prepared from soaking of soybean in NaOH solution at high temperature were deep yellow but were high whiteness in soybean milk prepared from soaking of soybean in water at low temperature. Sensority of soybean milk prepared from soaking of soybean in 0.1% of NaOH solution at $90^{\circ}C$ for 1 hr was more favorable than the others.

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Volatile Components in the Soy Sauce Manufactured by Bacillus Species and Fused Yeast

  • Kim, Haeng-Ja;Lee, Eun-Ju;Shin, Ok-Sun;Ji, Won-Dae;Choi, Myeong-Rak;Kim, Jong-Kyu
    • Journal of Microbiology and Biotechnology
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    • v.6 no.3
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    • pp.194-201
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    • 1996
  • To develop a method appropriate for mass production in a factory, we manufactured soy sauce with Bacillus species SSA3-2M1 and fused ST723-F31 at $30^{\circ}C$ with aeration of 1/3 vvm for 40 days. The flavor components extracted from the manufactured soy sauce were fractionated to neutral, acidic, basic and phenolic fraction and identified by GC-mass. Among the 60 kinds of identified flavor components, 16 and 23 components were detected in traditional Korean soy sauce and soybean paste, respectively. There were three peak regions that smelled like soy sauce with the GC sniffing test of flavor components and 2, 6-dimethyl pyrazine, benzaldehyde, 2-methoxy phenol, phenol and benzeneethanol which were identified as character impact compounds of traditional Korean soy sauce and soybean paste were identified in the region that smelled like soy sauce. It is therefore considered possible to achieve mass production of soy sauce with standard quality by Bacillus species SSA3-2M1 and fused ST723-F31 in the factory.

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Dyeing of Cotton and Polyester/Cotton Blend with Disperse Dyes Using Sodium 2-(2,3-dibromopropionylamino)-5-(4,6-dichloro-1,3,5-triazinylamino)-benzenesulfonate

  • Kim, Mi-Kyung;Yoon, Seok-Han;Kim, Tae-Kyeong;Bae, Jin-Seok;Yoon, Nam-Sik
    • Fibers and Polymers
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    • v.7 no.4
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    • pp.352-357
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    • 2006
  • The cotton fabrics were pretreated by sodium 2-(2,3-dibromopropionylamino)-5-(4,6-dichloro-1,3,5-triazinylamino) benzenesulfonate (DBDCBS) at alkaline condition ofroom temperature and then dyed with four disperse dyes having amino groups (C.I. Disperse Yellow 9, C.I. Disperse Red 11, C.I. Disperse Blue 56 and C.I. Disperse Violet 1) at acidic condition of high temperature. A novel hetero-bifunctional bridge compound, DBDCBS, has two reactive groups such as dichloro-striazinyl group and ${\alpha},\;{\beta}$-dibromopropionylamido group. The first has reactivity towards hydroxy group of cellulosic fiber and the second shows reactivity towards amino groups of disperse dye containing amino groups. The results indicate that it is possible to dye polyester/cotton blend at one-bath dyeing using one kind of disperse dye containing amino groups. Therefore, two kinds of dyeing methods such as two-bath process one-bath dyeing and one-bath process one-bath dyeing were investigated and their dyeabilities were compared. The differences between these two methods were negligibly small so that perfect one-bath one-step dyeing of polyester/cotton blend by one kind of disperse dye was achieved.

Chemical Analysis of Transplanted Aquatic Mosses and Aquatic Environment during a Fish Kill on the Chungnang River, Seoul, Korea

  • Lee, Joohyoung;Green, Perry-Johnson;Lee, Eun-Ju
    • Animal cells and systems
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    • v.6 no.3
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    • pp.215-219
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    • 2002
  • In mid-April, 2000, hundreds of thousands of fish floated dead on the Chungnang River, one of the small branches of the Han River in Seoul. We examined the causes of the accident in detail, through analysis of monitorinq data from the Han River Monitoring Project, which employed the transplanted aquatic moss, Fontinalis antipyretica. This allowed investigation of another possible cause of the fish kill: release of trace metals into the river from industrial sources during the rainfall event. In addition, we aimed to verify the usefulness of aquatic mosses as bioindicators of the event. Water samples collected 48 h after the fish kill exhibited low pH and high Total-N and Total-p, indicating that acidic compounds rich in nitrogen and phosphorus might be a major contaminant. BOD and COD were also very high. On the whole, the conditions of the river water were degraded at that time. Distinct trends were not observed in the chlorophyll phaeophy-tinization quotient and photosynthesis rate of transplanted mosses. How-ever mosses sampled soon after the accident exhibited the lowest values for those variables (P < 0.01), suggesting that stress factors in the river were diluted out over time. Heavy metals with characteristics of industrial effluents (Cr, Pb, Zn, Fe, Cu, and Cd) increased (p < 0.01), indicating that they were unlikely to be major causes of the accident.

Adsorption properties of activated carbon prepared from pre-carbonized petroleum coke in the removal of organic pollutants from aqueous solution

  • Ahmed, S.A. Sayed;El-Enin, Reham M.M. Abo;El-Nabarawy, Th.
    • Carbon letters
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    • v.12 no.3
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    • pp.152-161
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    • 2011
  • Activated carbon was prepared from pre-carbonized petroleum coke. Textural properties were determined from studies of the adsorption of nitrogen at 77 K and the surface chemistry was obtained using the Fourier-transform infrared spectrometer technique and the Boehm titration process. The adsorption of three aromatic compounds, namely phenol (P), p-nitrophenol (PNP) and benzoic acid (BA) onto APC in aqueous solution was studied in a batch system with respect to contact time, pH, initial concentration of solutes and temperature. Active carbon APC obtained was found to possess a high surface area and a predominantly microporous structure; it also had an acidic surface character. The experimental data fitted the pseudo-second-order kinetic model well; also, the intraparticle diffusion was the only controlling process in determining the adsorption of the three pollutants investigated. The adsorption data fit well with the Langmuir and Freundlich models. The uptake of the three pollutants was found to be strongly dependent on the pH value and the temperature of the solution. Most of the experiments were conducted at pH 7; the $pH_{(PZC)}$ of the active carbon under study was 5.0; the surface of the active carbon was negatively charged. The thermodynamic parameters evaluated for APC revealed that the adsorption of P was spontaneous and exothermic in nature, while PNP and BA showed no-spontaneity of the adsorption process and that process was endothermic in nature.