• Title/Summary/Keyword: acid values

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Characteristic of back fat and quality of longissimus dorsi muscle from soft fat pork carcasses

  • Lim, Daewoon;Song, Minho;Lee, Juri;Lee, Chulwoo;Lee, Jaechung;Lee, Wangyeol;Seo, Jihee;Jung, Samooel
    • Korean Journal of Agricultural Science
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    • v.43 no.4
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    • pp.581-588
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    • 2016
  • The objective of this study was to investigate the accuracy of visual discrimination of soft fat pork carcasses when subjecting carcasses to quality grade evaluations. In addition, the quality of the longissimus dorsi muscle from soft fat carcasses was investigated. Iodine values of back fat from soft fat carcasses evaluated by visual discrimination were significantly higher than those from firm fat carcass (p < 0.05). However, those values were lower than the standard for soft fat (iodine value = 70). There were no significant differences in linoleic acid content, b-values, and L-values (p < 0.05) of back fat between firm and soft fat carcasses evaluated by visual discrimination. Color of longissimus dorsi muscle from soft fat carcasses (iodine value higher than 70) was not different from that of firm fat carcass (iodine value lower than 70). Except for linoleic acid, there were no significant differences in any fatty acid contents between longissimus dorsi muscles from firm fat and soft fat carcasses. Monounsaturated fatty acid content of longissimus dorsi muscles from soft fat carcasses was significantly lower than those of firm fat carcass (p < 0.05). However polyunsaturated fatty acid content was significantly higher (p < 0.05) in longissimus dorsi muscles from soft fat carcasses. In conclusion, visual discrimination results for soft fat pork carcass were inaccurate. Therefore, other indicators should be required to evaluate soft fat pork carcasses. In contrast, the quality of longissimus dorsi muscle from soft fat carcasses was superior in terms of fatty acid composition compared with that of firm fat carcasses.

Effects of oil refining processes on oil characteristics and oxidation stability of sesame oil (정제공정이 참기름의 유지특성과 산화안정성에 미치는 영향)

  • Han, Jin-Suk;Ahn, Seung-Yo
    • Applied Biological Chemistry
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    • v.36 no.4
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    • pp.284-289
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    • 1993
  • The effect of oil refining processes-degumming, alkali-refining, bleaching and deodorizing-on oil characteristics and oxidation stability of sesame oil were investigated. The colors(L, a, b) of samples were markedly changed and their peroxide and acid values were decreased, while the other characteristics of samples were not changed during refining stages. The L values of alkali-refined, bleached and deodorized sesame oils were largely decreased and their a values were increased due to browning reaction during the storage at $70^{\circ}C$. The colors of crude and degmmed sesame oils were very stable and their peroxide, free fatty acid and conjugated dienoic acid values were slowly increased. Volatile carbonyl compounds formed by oxidation were increased during the storage at $70^{\circ}C$. The results indicated that refining processes did not affect the sesame oil characteristics but decreased the oxidation stability of sesame oil.

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The Influence of pH on the Color Development of Melanoidins Formed from Fructose/Amino Acid Enantiomer Model Systems

  • Kim, Ji-Sang;Lee, Young-Soon
    • Preventive Nutrition and Food Science
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    • v.13 no.4
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    • pp.306-312
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    • 2008
  • This study investigated the influence of pH on the color development of melanoidins formed from amino acid enantiomer model systems. For this, the color development was evaluated by measuring browning at 420 nm and color measurements by spectrophotometry and colorimetry. The browning and browning index showed no difference according to the type of amino acid enantiomers, while that formed from the D-Asn system was the only difference according to pH level. The tristimulus value of melanoidins formed from all model systems was located on a dominant wavelength of 475 nm, the blue zone of the diagram. In addition, the $L^*$, $a^*$, $b^*$, $C^*_{ab}$ values, and ${\Delta}E^*$ index on the basis of the type of amino acid enantiomers, the differences were markedly found at pH 4.0. At pH 7.0, significantly differences were found in the $L^*$, $a^*$, $b^*$ values, and ${\Delta}E^*$ index and not in the case of the lysine enantiomers. In addition, at pH 10.0, the differences were found in the $a^*$ and $b^*$ values from the lysine enantiomers and $C^*_{ab}$ value from the asparagine enantiomers. Therefore, the color development of melanoidins was influenced by the type of amino acid enantiomers and pH levels. Especially, it is thought that the $a^*$ and $b^*$ values can be used to explain the differences among the amino acid enantiomers in the color development of melanoidins.

Effect of Caffeic Acid on the Production of Reactive Oxygen Species in Raw 264.7 Cells (Raw 264.7 세포에서 유해산소 생성에 미치는 Caffeic Acid의 영향)

  • Choi, Byung-Chul
    • YAKHAK HOEJI
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    • v.52 no.6
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    • pp.441-445
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    • 2008
  • To investigate effect of caffeic acid on the intracellular reactive oxygen species production, we used DHE for intracellular superoxide anion production, DCF for intracellular ${H_2}{O_2}$ production and DHR for intracellular hydroperoxide production in Raw 264.7 cells. DPPH assay showed that antioxidant activity of caffeic acid with 39.5 ${\mu}M$ of ${IC}_{50}$ values was similar to that of ascorbic acid with 41.3 ${\mu}M$ of ${IC}_{50}$ values. Caffeic acid dose-dependently inhibited silica-induced ${H_2}{O_2}$ and hydroperoxide production but did not affect superoxide anion production in Raw 264.7 cells, which suggest that antioxidant effect of caffeic acid acts on the post-step of superoxide anion. On the other hand, caffeic acid showed a potent antioxidant effect in $lCuSO_4$-induced lipid peroxidation. Furthermore, plasma superoxide dismutase activity (3.43${\pm}$0.23 U/ml) in 10 mg/kg caffeic acid-fed mice was significantly higher than that (2.32${\pm}$0.24 U/ml) of control. From the above results, it is referred that caffeic acid appears to have potent anti-oxidant activity in both cell system and in vivo system.

The pH Studies of Recombinant Acetohydroxy Acid Synthase from Tobacco

  • Choi, Jung-Do;Kim, Bok-Hwan;Yoon, Moon-Young
    • Bulletin of the Korean Chemical Society
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    • v.24 no.5
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    • pp.627-632
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    • 2003
  • The pH dependence of the kinetic parameters of recombinant acetohydroxy acid synthase catalyzed reaction was determined in order to obtain information about the chemical mechanism, particularly acid-base chemistry. The maximum velocity and V/K for pyruvate were bell-shaped with estimated pK values of 6.5-6.7 and 8.6-8.9, respectively. The maximum velocity and V/K for 2-ketobutyrate were also bell-shaped with estimated pK values of 6.6-7.0 and 8.4-8.6. The pH dependence of 1/Ki for 3-bromopyruvate, a competitive inhibitor of pyruvate, was also bell-shaped, giving pK values almost identical with those obtained for pyruvate. Since the same pK values were observed in the $pK_{i 3-bromopyruvate}$, V/K pH profiles and $V_{max}$ profiles, both enzyme groups must be in their optimum protonation state for efficient binding of reactants. These results reflect that two enzyme groups are necessary for binding of substrate and/or catalysis.

Flash Points of the Binary Solutions Using Cleveland Open Cup Tester (클리브랜드 개방식 장치를 이용한 2성분계의 인화점)

  • Ha, Dong-Myeong;Lee, Sung-Jin
    • Fire Science and Engineering
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    • v.25 no.1
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    • pp.57-62
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    • 2011
  • The flash point is one of the most significant combustion properties of flammable liquids in industrial processes when evaluation process safety, In this paper, Cleveland open cup tester is used to measure the flash points for the two binary systems (n-propanol + formic acid and acetic acid + propionic acid). The measured flash points were compared with the values calculated by the Raoult's law and the optimization method using van Laar and Wilson equations. The calculated values based on the optimization method were found to be better than those based on the Raoult's law.

Effects of Dietary illite As a Feed Additive on The Haugh Unit and Fatty Acid Profiles of Eggs (일라이트(illite)를 산란계 사료에 첨가 시 계란 신선도와 지방산 조성에 미치는 영향)

  • Choi, In-Hag
    • Journal of Environmental Science International
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    • v.28 no.9
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    • pp.807-811
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    • 2019
  • The present study aimed to examine the effects of dietary illite as a feed additive on the Haugh unit and fatty acid profiles of eggs. One hundred and twenty laying hens (48-week-old Hy-line Brown) were randomly assigned to one of the following two treatments: control or 2% illite powder. At 2 and 4 weeks, the Haugh unit values between the control and 2% illite powder groups were not significantly different (p>0.05). In addition, individual fatty acid content at 2 and 4 weeks did not differ between the treatments (p>0.05), except for palmitic (C16:0) and palmitoleic acid (C16:1) content. The addition of 2% illite powder caused no difference between the total saturated and total unsaturated fatty acids at 2 and 4 weeks (p>0.05). In conclusion, the addition of dietary illite at a rate of 2% did not improve the Haugh unit values or fatty acid profiles of eggs.

Effect of dietary betaine on short chain fatty acid and blood profile in meat duck exposed to extreme heat stress (베타인이 폭염 오리의 짧은 사슬지방산 및 혈액 프로파일에 미치는 효과)

  • Hwangbo, Jong;Bang, Han-Tae;Park, Byung-Sung
    • Journal of the Korean Applied Science and Technology
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    • v.32 no.3
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    • pp.394-404
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    • 2015
  • This study investigated the pharmacodynamics of betaine on the blood profile and short chain fatty acid levels in meat ducks exposed to heat wave. 400 heads of Cherry valley (Anasplatyrhynchos) meat ducks were completely randomized to 5 treatments (4 repetitions each), and were raised for 42 days. They were grouped into T1 (heat wave control group without betaine), T2 (betaine 400 ppm), T3 (betaine 800 ppm), T4 (betaine 1200 ppm), and T5 (normal control group without betaine). Compared to T1, the betaine addition groups showed higher body weight gain at shipment, with T3 showing the highest significant difference. For hematological indictors measured (red blood cells and platelets), the betaine addition groups showed significantly higher values than the heat wave control group. The pH of the former was lower but their electrolytes ($K^+$, $P^+$, and $Cl^-$) were significantly higher compared to the latter. For blood gas concentration, the former showed a significantly higher value than the latter. For the total short chain fatty acids, acetic acid, and propionic acid, the betaine addition groups and group fed broiler-high temperature diet showed higher values than the heat wave control group. On the other hand, the former showed significantly lower values in butyric acid, isobutyric acid, valeric acid, and isovaleric acid than the latter group. These results suggest that betaine has the pharmacodynamics that mediate heat stress, via the maintenance and control of the blood profile, osmotic pressure, gas concentration, and short chain fatty acid, of meat ducks under heat wave.

Quality Characteristics of Tteokgalbi with Black Rice Bran and Organic Acid to Substitute Synthetic Caramel Colorant

  • Kim, Tae-Kyung;Hong, Seung-Hee;Ku, Su-Kyung;Kim, Young-Boong;Jeon, Ki-Hong;Choi, Hee-Don;Park, Jong-Dae;Park, Kwoan-Sik;Choi, Yun-Sang
    • Food Science of Animal Resources
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    • v.37 no.4
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    • pp.552-560
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    • 2017
  • This study aimed to evaluate the quality characteristics of Tteokgalbi with 1% (w/w) black rice bran only (T1), or with black rice bran and one of the following four types of organic acid: ascorbic acid (T2), citric acid (T3), tartaric acid (T4), or maleic acid (T5) as a substitute for caramel colorant. Tteokgalbi with only black rice bran showed the highest (p<0.05) values of moisture content and water holding capacity (WHC), while there were no significant differences in protein content, fat content, ash content, and thiobarbituric acid reactive substance (TBARS) values in treatments and controls (p>0.05). All the treated samples with any one of the four organic acids showed lower pH than controls (p<0.05). The lightness and redness of Tteokgalbi treated with any one of the four organic acids and black rice bran were higher than those of T1. The volatile basic nitrogen (VBN) values of T4 and T5 were higher than those of the other treatments (p<0.05). With regards to sensory characteristics, T1 and T2 showed overall acceptability similar to that of the controls (p>0.05). The results reported in this study show that Tteokgalbi with black rice bran and any one of the four organic acids listed above not only improved quality characteristics in cooking loss, WHC, lipid oxidation but also could successfully replace the synthetic caramel colorant. Overall, the most satisfactory results were obtained by adding black rice bran and ascorbic acid.

Determination of Fatty Acid Composition in Peanut Seed by Near Infrared Reflectance Spectroscopy

  • Lee, Jeong Min;Pae, Suk-Bok;Choung, Myoung-Gun;Lee, Myoung-Hee;Kim, Sung-Up;Oh, Eun-young;Oh, Ki-Won;Jung, Chan-Sik;Oh, In Seok
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.61 no.1
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    • pp.64-69
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    • 2016
  • This study was conducted to develop a fast and efficient screening method to determine the quantity of fatty acid in peanut oil for high oleate breeding program. A total of 329 peanut samples were used in this study, 227 of which were considered in the calibration equation development and 102 were utilized for validation, using near infrared reflectance spectroscopy (NIRS). The NIRS equations for all the seven fatty acids had low standard error of calibration (SEC) values, while high R2 values of 0.983 and 0.991 were obtained for oleic and linoleic acids, respectively in the calibration equation. Furthermore, the predicted means of the two main fatty acids in the calibration equation were very similar to the means based on gas chromatography (GC) analysis, ranging from 36.7 to 77.1% for oleic acid and 7.1 to 42.7% for linoleic acid. Based on the standard error of prediction (SEP), bias values, and $R^2$ statistics, the NIRS fatty acid equations were accurately predicted the concentrations of oleic and linoleic acids of the validation sample set. These results suggest that NIRS equations of oleic and linoleic acid can be used as a rapid mass screening method for fatty acid content analysis in peanut breeding program.