• Title/Summary/Keyword: acid soaking

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Analysis of Genome and Species Relationships in Cotton by Disc Electrophoresis (Disc Electrophoresis에 의한 면화의 Genome과 종간관계 분석)

  • Jong-Tol Choi;Byong-Ho Chang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.13
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    • pp.73-76
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    • 1973
  • Protein spectra from 6 species of Gossypium were obtained by disc electraphoresis of seed extracts. Protein extracts were made by soaking 0.5g. of seed in 15ml of Tris-glycine buffer for 24 hours. Gels 24 hours. Gels were stained in 0.5% Amido Black solution for 1 hour, and destained in 7% acetic acid for 72 hours. Nine to 15 bands were visible in each gel. Homologies of Protein bands among the species were determined by migration velocity. Evidences obtained from electrophoretic separation of seed Protein were consistent with those from genetic, cytological, morphological and Phenogenetic methods regarding the origin of New World cultivated cottons. Possibility, however, does not exist to exclude Gossypium herbaceum from one of the Progenitors of New World cultivated cottons from electrophoretic evidences alone.

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The Properties of Blanching and Brining in Hot Solution and Trehalose Treatment on the Quality of Cucumber Kimchi during Fermentation (데침과 열수의 침적과 Trehalose 처리가 오이 물김치의 저장중 품질에 미치는 영향)

  • 이혜정
    • The Korean Journal of Food And Nutrition
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    • v.14 no.4
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    • pp.333-338
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    • 2001
  • In research. which Korean pickled cucumber was treated wish various methods, including blanching. brining in hot solution, and treating with trehalose. I examined the changes of properties of material. The Korean pickled cucumber were fermented 4∼5$^{\circ}C$ for 42 days in 1% salt solution. The physiochemical properties were pH, total acidity, total cell count, lactic acid bacteria and texture properties were also evaluated. The result showed that the effect of blanching and soaking cucumber in 100$^{\circ}C$ hot salt solution significantly reduced the softening rate of texture while a rather rapid fermentation was found for those preserved with salt. The effect of trehalose treatment inhenced fermentation but it was significantly reduced softening rate of texture. The texture evaluation of Korean pickled cucumber was found that heat treatment with blanching after soaked in hot solution and trehalose treatment had a positive effect for reduction of softening of cucumber tissue.

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Preparation of Traditional Malt-Sikhye - 2. Preparation by Malt (전통식혜제조-제 2보 엿기름에 의한 제조)

  • 안용근
    • The Korean Journal of Food And Nutrition
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    • v.12 no.2
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    • pp.170-176
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    • 1999
  • Optimum preparation conditions of Korean traditional sweet rice drink 'malt-Sikye' were 1hour of rice soaking time 30min of malt extraction time 60$^{\circ}C$ of malt extraction tem-perature 60$^{\circ}C$ of saccharification temperature 1hour of agitation interval 5hour of saccharification time 5.5 of pH 4% of malt concentration and 20% of rice content. The malt-Sikhy contained to 17.1% of total sugar 11.2% of reducing sugar o.34mg/ml of protein and 4.7${\mu}$mol/ml of amino acid. The most abundant sugar found in malt-Sikhye was maltose. The commercial amylolytic enzymes were not effec-tive in preparation malt-Sikye.

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A Plan for Improving Quality of Traditional Soybean Paste (전통된장의 품질개선에 관한 연구)

  • 최동원
    • The Korean Journal of Food And Nutrition
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    • v.16 no.3
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    • pp.218-223
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    • 2003
  • This study is proceeded on the development of standard method for making soybean paste by Korean traditional method. Fermentation condition of Meju was 1) Pre-fermentation : 30 days in about 20$^{\circ}C$ room, 2) Main fermentation : 5 days in 30$^{\circ}C$ or upper temperature, 3) Post fermentation and drying : 30 days in well sunlightened room in January. Meju was soaked in 18% salt solution(Meju 7kg/salt solution 20L) for 35~40 days (from late February to early April) and after soaking Meju was filtered as unsoluble solute and crushed and put into traditional Korean receptacle(named 'Dok'). Crushed Meju was stored from early April to mid September and Meju was changed into soybean paste(Doen-jang). During fermentation amino acid nitrogen in Doen-jang was slightly increased in early period and decreased lately. It has been proved that by panel test soybean paste made by the method suggested in this study was more excellent than commercially fermented soybean paste. This study has presented the possibility of commercial production of soybean paste made by traditional method.

Effect of pyrolysis temperature on the thermal energy storage performance of sesame plant biochar (참깨 바이오차의 열분해 온도가 열에너지 저장 성능에 미치는 영향 평가)

  • Soumen Mandal;Lee, Han-Seung
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2023.11a
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    • pp.65-66
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    • 2023
  • This study is aimed to understand the effect of pyrolysis temperature on the biochar synthesis for shape stabilizing the Lauric acid (LA). Three different temperatures (350, 500, and 650 ℃) have been chosen with a soaking time of 1h for synthesizing biochars form sesame plants (SP). The structural characterizations indicate the formation of amorphous biochar at 350 ℃ whereas, a stain of graphene formation has been observed for the biochar synthesized at 500 ℃. Formation of a substantial amount of graphene has been found for the sample, synthesized at 650 ℃. Energy storage performances of the PCMs which are shape stabilized by these three biochars have been discussed in this paper.

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Comparison of pretreatment of fallen leaves for application evaluation by Bio-ethanol raw material (바이오에탄올 원료로서 활용평가를 위한 낙엽의 전처리 비교)

  • Choi, Hyoyeon;Kim, Jaehyung;Pak, Daewon
    • Journal of Energy Engineering
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    • v.23 no.3
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    • pp.241-246
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    • 2014
  • This study is to compare characteristics of saccharification reactions applying to enzymatic hydrolysis of pretreated fallen leaves for bio-ethanol production. It experimented pretreatment of acid, alkaline in the chemical. This experiment includes preteatment of acid and alkaline in chemical, soaking, shaking and autoclaving method, which were applied to biomass. In result, the glucose production from alkaline-NaOH method was 263 mg glucose/ g biomass comparing with them of acid-HCl method. Thus, alkaline-NaOH method is superior than the acid-HCl method for chemical preteatment of fallen leaves. Also, when various chemical treatments were compared, they were all. Based on the results of this study, we found that leaves, one of biomass, are possible in pretreatment and enzymatic hydrolysis process, and they are likely to affect bio-ethanol production in the future.

Associated Microorganisms and Chemical Composition of Persimmon Pickles (감장아찌의 성분특성 및 관련 미생물)

  • Chung, Dong-Ok;Chung, Hee-Jong
    • Journal of the Korean Society of Food Culture
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    • v.10 no.3
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    • pp.133-137
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    • 1995
  • The objectives of this study were to determine the chemical composition of persimmon pickles and microorganisms associated with the manufacture of persimmon pickles. Moisture content was 80.04% and sugar content was 13.72%, but crude ash content was 4.26% which meant some of sodium chloride in soaking solution were permeated into fresh persimmon. Soluble tannin and total vitamin C contents were decreased from 635.45 mg% and 34.53 mg% to 81.04 mg% and 23.76 mg%, respectively. Free sugars contained in persimmon pickles were glucose, fructose and sucrose, 17 kinds of free amino acids were detected including glutamic acid (24.97 mg%), aspartic acid (24.02 mg%) and leucine (22.33 mg%). Microorganisms asociated with the manufacture of persimmon pickles were identified as Bacillus subtilis, B. cereus, B. pumilus, B. thuringiensis, Candida glabrata, C. guilliermondi and Hansenula cnomala, especially C. glabrata was the strain which caused the swelling of vacuum packages of persimmon pickles in retail stores.

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Establishment of Seed Treatment for Healthy Production of Peanut Sprout (청정 땅콩나물 생산을 위한 종자처리기술 확립)

  • Park, Eun-Ji;Lee, Gyu-Bin;Heo, You;Son, Beung-Gu;Choi, Young-Whan;Lee, Yong-Jae;Park, Young-Hoon;Suh, Jeong-Min;Kang, Jum-Soon
    • Journal of Environmental Science International
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    • v.24 no.6
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    • pp.755-762
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    • 2015
  • The present study was conducted to develop seed treatment for the production of healthy and clean peanut sprout. Dry heat treatment of peanut seeds reduced the incidence of the rot. The seed treatment condition at $52^{\circ}C$ for 10 h. was the most efficient without inhibiting seed viability significantly. Seeds were dark cultured at $27^{\circ}C$ for up to 9 days. The treatment of Indole-B and gibberellic acid influenced germination, T50, fresh, dry weight, hypocotyl length, hypocotyl length diameter, root length, number of lateral root and epicotyl of peanut sprout. There were no differences in the germinability of peanut seeds between gibberellic acid treatment methods but higher fresh weight was observed in the GA3 solution spray after 2 hour water soaking. The general growth and lateral root development of peanut sprouts were suppressed by Indole-B which is used for inhibiting root formation and promoting hypocotyls. The treatment of gibberellic acid promoted hypocotyl elongation, but it did not influence on the growth of hypocotyls and root system.

Effects of Heat Treatment and Selected Medicinal Plant Extracts on GABA Content after Germination (열처리와 한약재 추출물이 발아현미의 GABA 함량에 미치는 영향)

  • Jeon, Geon-Uk;Lee, Mi-Young;Yoon, Jae-Min;Jang, Sung-Ho;Jung, Mi-Ri;Jeong, Heon-Sang;Lee, Jun-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.1
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    • pp.154-158
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    • 2010
  • The purposes of this work were to study germination conditions on $\gamma$-aminobutyric acid (GABA) contents and to develop a simple and effective technique for the production of germinated brown rice with an enhanced GABA levels. The samples were subjected to heat treatments at 40 and $45^{\circ}C$ before the soaking step. Moreover, four medicinal plant extracts including Schizandra chinensis, Dimocarpus longan Lour, Angelica dahurica, and Gastrodia elata Blume were used as soaking and germinating media. GABA levels were enhanced in the germinated brown rice compared to the non-germinated brown rice and the highest GABA contents were observed in heat treatment at $35^{\circ}C$. The brown rice soaked in Gastrodia elata Blume extract showed the highest GABA contents compared to the control sample. These results demonstrate that the GABA levels during germination could be significantly enhanced by the heat treatment and the treatment of medicinal plant extracts in the soaking step.

CONDITIONS FOR PROCESSING OF MEATY TEXTURED FISH PROTEIN CONCENTRATE FROM ALASKA POLLACK AND MACKEREL (명태 및 고등어의 축육과 유사한 어육조직단백질 농축물의 가공조건)

  • LEE Eung-Ho;KIM Se-Kwon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.12 no.2
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    • pp.103-111
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    • 1979
  • For the effective utilization of the coastal fish resources in Korea, an investigation on the optimum processing conditions and the quality of a textured fish protein concentrate similar to the texture of animal meat has been carried out with the fish meat of Alaska pollack and mackerel. A noodle shaped product was prepared with the fish meat paste after the adjustment of pH and salt content. The product was soaked in $96\%$ ethyl alcohol to produce textured fish protein concentrate and then dried. The processing conditions were estimated with the rehydration capacity of the textured fish protein concentrate(FFC). The quality of the final product was evaluated with chemical composition, sensory test and texture measurement. The optimum pH and salt content of the fish meat for the processing of meaty textured FPC were 7.5 and $1.0\%$ respectively. The most effective soaking conditions were as follows:soaking time, 40 min. ; temperature of alcohol, 5 to $20^{\circ}C$;amount of alcohol, 4 times the weight of tile fish meat paste, number of soaking in alcohol, 4 times. The alcohol remaining in meaty textured FPC could be removed effectively by forced air drying. The yield and the contents of protein and lipid in the meaty textured FPC from Alaska pollack were $19.9\%\;84.3\%\;and\;0.5\%$ and those from mackerel were $29.8\%,\;78.1\%\;and\;3.6\%$ respectively. The content of essential amino acid in the meaty textured FPC from Alaska pollack and mackerel was not inferior to that of beef, textured soybean protein and FAO pattern. Beef meat can be substituted with the meaty textured FPC up to $50\%$ in processing meat balls withoutanysignificantlossinthetaste, ordor and texture.

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