• 대한수의학회지
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• 제43권3호
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• pp.517-520
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• 2003

Prevalence of microfilariae, Dirofilaria immitis, Dirofilaria repens, Acanthocheilonema dracunculoides from 506 German Shepherd dogs reared in Korea was investigated by modified Knott's test to detect circulating microfilaria and by acid-phosphatase staining for differentiation of each microfilaria species. In the modified Knott's test, 74 of 506 dogs (14.6%) were microfilaria positive, and the prevelance of each species of microfilaria was 90.5% (67 of 74 samples) for D. immitis, 5.4% (4 of 74 samples) for duplicate infection with D. immitis and D. repens and 4.1% (3 of 74 samples) for mixed infection with D. immitis, D. repens and A. dracunculoides. It was considered that the paying attention to the existence not only D. immitis but also other microfilariae were needed in canine filariasis.

• Journal of Korean Dental Science
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• 제5권1호
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• pp.37-44
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• 2012

Purpose: Among the facilitation of tooth movement in adult orthodontic treatment methods, surgical approaches are gaining popularity but complications following mechanical bone reduction are a problem. In this study, tooth movement was observed after alveolar bone was chemically demineralized to verify whether tooth movement had been facilitated. Materials and Methods: Twelve rabbits were used. In the experimental group, the alveolar bone of the left first molar area was exposed and demineralized. Thirty seven percents phosphoric acid was applied for 5 minutes for demineralization. The opposite first molar area was used as control. Two teeth were pulled with 200 g force and 4 rabbits each were sacrificed at 3, 7, and 14 days after the force was applied. Histologic examination was done with hematoxylin and eosin and tartrate-resistant acid phosphatase staining. Result: The histologic examination results revealed more bone resorption in the demineralized area. As time passed, the number of osteoclasts increased in the compressed area. The amount of tooth movement was larger in the experimental group compared to the control group but the difference was not statistically significant. Conclusion: The demineralization with etchant resulted in limited bone resorption, more tooth movement and less damage of the cementum after applied orthodontic force.

• Animal cells and systems
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• 제14권2호
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• pp.73-81
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• 2010

In this study, monoclonal antibodies against lysosomal acid phosphatase (LAP) of a salamander, Hynobius leechii, were used to determine the spatial and temporal expression of the LAP in the regenerating limbs. The Western blot and immunohistochemical analysis in the limb regeneration revealed that LAP was highly expressed at the dedifferentiation stage, especially in the wound epidermis and dedifferentiating limb tissues such as muscle and cartilage. With RA treatment, the LAP expression became upregulated in terms of both level and duration in the wound epidermis, blastemal cell and dedifferentiating limb tissues. In addition, in situ activity staining of LAP showed a similar result to that of immunohistochemistry. Thus, the activity profile of LAP activity coincides well with the expression profile of LAP during the dedifferentiation period. Furthermore, to examine the effects of lysosomal enzymes including LAP on salamander limb regeneration, lysosome extract was microinjected into limb regenerates. Interestingly, when the lysosome extract was microinjected into limb regenerates with a low dose of RA($50\;{\mu}g/g$ body wt.), skeletal pattern duplication occurred frequently in the proximodistal and transverse axes. Therefore, lysosomal enzymes might cause the regenerative environment and RA plays dual roles in the modification of positional value as well as evocation of extensive dedifferentiation for pattern duplication. In conclusion, these results support the hypothesis that dedifferentiation is a crucial event in the process of limb regeneration and RA-evoked pattern duplication, and lysosomal enzymes may play important role(s) in this process.

• 대한치과의사협회지
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• 제9권5호
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• pp.235-239
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• 1971

The effects of folic acid on the oral mucosa of albino rats were histochemically studied in the twenty four male rats, weighing abut 100gm. Seven groups of 3 each were injected with 0.2ml folic acid (folic acid 0.5mg. was dissolved in physiological saline 0.2ml) subcutaneously, during 1,2,3,5,7,10 and 14 days respectively. Oral mucosa of rats sere removed from upper molar region and fixed in 10% formalin, cold absolute alcohol, Carnoy's solution and acetone. The serial sections were histochemically stained by McManu's PAS reaction, Mowry's metachromasia, alloxan-Schiff reaction, and azo dye method for alkaline phosphatase. The comparative staining method was hematoxylin-eosin stain. The results were as follows: 1) Alkaline phosphatase reaction of stratum spinosum and stratum granulosum tended to increase after 7 and 10 days of folic acid administration. 2) PAS reactions of basement membrane and lamina propria increased after folic acid administration. 3) Metachromasia of stratum spinosum and stratum granulosum were slightly increased after 3,5 and 7days of folic acid administration and returned to the level of Control after 10 days. 4) In the oral mucosa, alloxan-Schiff reaction increased after 7 days of folic acid administration.

• 한국어병학회지
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• 제22권3호
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• pp.305-316
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• 2009

Haemocyte characterization in the surf clam, Mectra veneriformis was carried out based on morphological, cytochemical, and phagocytic characteristics. The haemocytes were classified into two cell types, granulocytes and agranulocytes on the basis of presence of cytoplasmic granules. Granulocytes were then classified again into 2 types, large eosinophilic granulocytes and small eosinophilic granulocytes after staining with May-Grünwald Giemsa. In electron microscopy, both types of granulocytes contained electron-dense and electron-lucent cytoplasmic granules. Agranulocytes (hyalinocytes) were also divided into two cell types, large agranulocytes and small agranulocytes based on their sizes. Both cell types did not have granules in cytoplasm. Granulocyte and agranulocyte were negative for the enzyme activities of alkaline phosphatase, peroxidase, and $\beta$-glucuronidase but positive for phenoloxidase and acid phosphatase activities. Both types of haemocytes have phagocytic activity, with the exception of small agranulocyte, and granulocytes seemed more active in this respect than agranulocytes. This present study is the first study to characterize haemocytes of M. veneriformis.

• 한국식품영양과학회지
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• 제12권2호
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• pp.84-92
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• 1983

자동 산화유가 mouse의 간장 효소에 미치는 영향을 구명하기 위하여 autoxidized methyl linoleate의 일정량을 mouse에게 1일 1회 20일 동안 경구 투여하여 간에서의 효소 활성을 조직화학적 및 생화학적 방법으로 검토한 결과는 다음과 같다. 실험군의 간에서의 POV, COV 및 TBA값은 정상군에 비해 크게 증가하여 AOML이 많이 흡수되어 있음을 알 수 있었다. 실험군의 간에서의 phospholipid, triglyceride 및 total cholesterol의 양은 정상군에 비해 증가하였다. 실험군의 간에서의 acid phosphatase, alkaline phosphatase의 활성은 정상군에 비해 증가하였으며, ATPase의 활성은 감소하였다. 실험군의 간에서의 형태학적 변화는 정상군에 비해 핵산의 감소, 지질의 축적 및 간 세포의 손상이 있었다. 이상의 결과로부터 mouse에 경구 투여한 자동 산화 methyl linoleate는 간에서의 효소 활성과 간의 형태학적 변화에 크게 영향을 주고 있음을 알 수 있었다.

• 한국임상수의학회지
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• 제20권4호
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• pp.443-448
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• 2003

본 연구는 전십자인대가 단열된 개의 슬관절액에서 tartrate-resistant acid phosphatase (TRAP) 와 cathepsin K의 변화를 알아봄으로서 십자인대에 관련된 질병 또는 십자인대 치료 실패에 따른 퇴행성관절염의의 조기 진단 및 치료에 관한 정보를 얻기 위하여 실시하였다. 실험동물로는 전방십자인대가 단열된 30두의 개와 정상 성견 8 두 그리고 어린 정상 개 9두를 사용하였다. 슬관절액 내의 TRAP 과 Cathepsin K의 변화를 확인하기 위하여 조직화학 염색과 면역조직화학염색을 실시하였다. 조직학적인 검사 결과 정상견에서는 TRAP과 Cathepsin K 세포들은 찾아보기 힘들었으나 전방십자인대가 단열된 개에 있어서는 TRAP 과 Cathepsin K 그리고 chondroid metaplasia가 중심부분에서 증가되고 있음을 관찰하였다. 이러한 결과로 보아 TRAP과 Cathepsin K 세포들은 전방십자인대가 단열 또는 단열 후 회복되는 과정에서 상호 보조적으로 관여하며 세포외 방출과 관계가 있는 것으로 생각된다.

• 한국한의학연구원논문집
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• 제16권1호
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• pp.149-155
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• 2010

Objective : Ssangwha-tang is a traditional medicine formula widely prescribed for a decrease of fatigue after an illness in Korea. The aim of this study is to investigate the effect of Ssangwha-tang fermented by Lactobacillus fermentum (SF) on osteoclast differentiation in vitro and on bone metabolism of an ovariectomized rat in vivo. Methods : Tartrate-resistant acid phosphatase activity and staining were applied to evaluate the formation of osteoclasts. Ovariectomized rats were orally administrated with SF (30 ml/kg/day) for 12 weeks. Serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total cholesterol, high-density lipoprotein-cholesterol, low-density lipoprotein-cholesterol, triglyceride, phosphate, calcium levels were determined. Effect of SF on bone loss were studied by histological analysis and the measurement of bone mineral density. Results : SF significantly inhibited tartrate-resistant acid phosphatase activity and formation of osteoclasts in RAW264.7 cells stimulated by receptor activator for nuclear factor ${\kappa}B$ (NF-${\kappa}B$) ligand (RANKL). In addition, SF significantly decreased the level of triglyceride and increased the level of low-density lipoprotein. Moreover, the decrease of trabeculae of the femur was partially prevented in ovariectomized rats administrated with SF. Conclusions : SF treatment could prevent ovariectomy induced bone loss and its effects could be medicated by the inhibition of osteoclastogenesis.

• 한국산림과학회지
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• 제68권1호
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• pp.32-36
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• 1985

소나무의 acid phosphatase (ACP), alcohol dehydrogenase (ADH)와 catalase (CAT) 동위효소(同位酵素) 유전양식(遺傳樣式)을 구명(究明)하기 위하여 배유조직(胚乳組織)을 수평식 감자전분 전기영동법에 의하여 분석(分析)하였다. ACT 동위효소(同位酵素)는 최소한 3~4개의 지역(地域)으로 분리(分離)되었으나 분리가 잘된 ACP-A 지역(地域)의 동위효소(同位酵素)만이 분석(分析)되었다. ACP-A 지역(地域)에서 관찰된 5개(A1-A5)의 동위효소(同位酵素) 표현형(表現型)들은 공히 Mendel의 분리비(分離比)를 보여 이들이 각각 ACP-A 유전자좌(遺傳子座)에 존재(存在)하는 5개의 대립유전자(對立遺傳子)에 의해 지배받고 있음을 알 수 있었다. 2개의 ADH 지역(地域)이 (ADH-A와 ADH-B) 분리(分離)되었으나, 양극(陽極)으로의 이동(移動)속도가 빠른 ADH-A 지역(地域)에서는 분석(分析)에 사용된 재료(材料)에서 변이(變異)가 발견(發見)되지 않았다. ADH-B 지역(地域)에서는 3개의 동위효소(同位酵素) 표현형(表現型) (B1-B3)들이 관찰되었고 이들이 공히 1:1의 분리비를 보여 ADH-B 유전자좌(遺傳子座)에 존재하는 3개의 대립유전자(對立遺傳子)에 의해 지배됨이 추정되었다. 수개의 band로 구성된 5개의 동위효소(同位酵素) 표현형(表現型)이 CAT에서 관찰되었으며, 이형접합성(異型接合性)인 모수(母樹)에서 이들 표현형간(表現型間)의 분리(分離)가 1:1 분리비(分離比)로부터 편차(偏差)를 보이지 않았으므로, 소나무에 있어서 CAT 동위효소(同位酵素)는 5개의 대립유전자(對立遺傳子)가 존재(存在)하는 하나의 유전자좌(遺傳子座)에 의해 지배되는 것으로 추정하였다.

• 한국산학기술학회논문지
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• 제11권9호
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• pp.3358-3365
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• 2010

본 연구에서는 마가목 (SC), 현지초 추출물 (GT) 및 이들의 1:1 혼합물 시료 (MIX)가 골손실 및 연골손상 억제에 효과가 있는지 알아보기 위해, 각각의 시료를 조골세포주인 MG-63 세포, 파골세포로의 분화를 유도한 Raw264.7 세포와 연골세포로의 분화를 유도한 ATDC5 세포에 처리하여 세포분화 조절 정도를 확인하였다. 각 세포의 분화 정도는 alkaline phosphatase (ALP) 활성 측정, tartrate-resistant acid phosphatase (TRAP) 염색법 및 alcian-blue 염색법으로 확인하였다. 이들 시료는 MG-63 세포에서 ALP 활성에는 영향을 미치지 않았으나, 마가목 추출물 (SC) 및 마가목과 현지초 추출물의 혼합시료 (MIX)는 농도 의존적으로 파골세포의 분화를 억제하고 연골세포의 분화를 촉진하는 것으로 나타났다. 이상의 결과를 종합하여 볼 때, 마가목과 현지초는 골손실과 연골 손상으로부터 보호할 수 있는 중요한 천연물 소재임을 확인할 수 있었다. 나아가 이들 추출물의 작용기전 및 활성물질 구명에 대한 연구는 추후 더 진행되어야 할 것이다.