• Title/Summary/Keyword: acetic acid production

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Effect of Acetic Acid on Bacteriocin Production by Gram-Positive Bacteria

  • Ge, Jingping;Kang, Jie;Ping, Wenxiang
    • Journal of Microbiology and Biotechnology
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    • v.29 no.9
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    • pp.1341-1348
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    • 2019
  • Acetic acid is indirectly involved in cell center metabolism, and acetic acid metabolism is the core of central metabolism, affecting and regulating the production of bacteriocin. Bacteriocin is a natural food preservative that has been used in the meat and dairy industries and winemaking. In this paper, the effects of acetic acid on bacteriocin produced by Gram-positive bacteria were reviewed. It was found that acetic acid in the undissociated state can diffuse freely through the hydrophobic layer of the membrane and dissociate, affecting the production, yield, and activity of bacteriocin. In particular, the effect of acetic acid on cell membranes is summarized. The link between acetic acid metabolism, quorum sensing, and bacteriocin production mechanisms is also highlighted.

Improvement of Ethanol Yield by Addition of Acetic Acid and Acetatdehyde in Ethanol Fermentation (에탄올 발효에서 초산 및 아세트알데히드 첨가에 의한 에탄올 수율의 증진)

  • 김진현;여주상유영제
    • KSBB Journal
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    • v.10 no.4
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    • pp.370-373
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    • 1995
  • The major by-products in ethanol fermentation by Saccharomyces cerevisiae were glycerol, acetaldehyde, acetic acid, lactic acid, and formic acid. The effects of these by-products on the cell growth and ethanol production were studied. By adding acetaldehyde or acetic acid in the fermentation broth, the cell growth decreased while the ethanol production increased. But glycerol and lactic acid had nearly no effects on the cell growth and the ethanol production. Acetic acid and acetaldehyde inhibited the cell growth by diminishing the growth rate as well as by prolonging the lag phase. The ethanol yield increased with the elevation of concentrations of acetic acid and acetaldehyde in the fermentation broth. The maximum ethanol yield was obtained for $3g/\ell$ acetic acid and $2g/\ell$ acetaldehyde, respectively.

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Effect of Acetic Acid on Xylitol Fermentation by Candiac parapsilosis (Candida parapsilosis에 의한 Xylitol 발효시 Acetic acid가 미치는 영향)

  • Kim, Sang-Yong;Yoon, Sang-Hyun;Kim, Jung-Min;Oh, Deok-Kun
    • Korean Journal of Food Science and Technology
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    • v.28 no.4
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    • pp.756-761
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    • 1996
  • Influence of acetic acid on xylitol production from xylose using Candida parapsilosis KFCC 10875 was investigated at the different concentrations of acetic acid. Acetic acid was totally consumed below 1.0 g/l of its concentration, whereas partially consumed above 3.0 g/l and remained in the medium during xylitol fermentation. Cell growth, xylose consumption, and xylitol production decreased when acetic acid concentration was increased. Specific growth rate of cell and specific consumption rate of xylose also decreased with increasing the concentration of acetic acid. However, the xylitol yield from xylose and specific production rate of xylitol were maximum at 1.0 g/l of acetic acid. The inhibitory effect of acetic acid on xylitol fermentation increased when pH was decreased.

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Effects of Concentration of Inhibitor on the Production of $\alpha$-amylase and Growth of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens 성장과 $\alpha$-amylase 생합성에 미치는 저해제의 영향)

  • 김종균;김종수차월석
    • KSBB Journal
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    • v.11 no.2
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    • pp.125-131
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    • 1996
  • In this study, Bucillus amyloliquefaciens was adopted as bacterial source to investigate the concentration of carbon source by adding inhibitors in the batch culture. By adding acetic acid at $10g/\ell$ of initial glucose concentration, maximum dry cell density was obtained with the highest value of /$\3.9gell$ at $1.0g/\ell$ of initial acetic acid concentration. By adding acetic acid al 10g/$\ell$ of initial glucose concentration, maximum ${\alpha}$-amylase production was obtained with 331.55unit/m1 at $2.0g/\ell$ of initial acetic acid concentration. ${\alpha}$-Amylase production was decreased with the increase of initial acetic acrid concentration. By adding acetic acid to the medium, cell growth and ${\alpha}$-amylase production was higher in glucose than in maltose. By adding lactic acid to the medium, cell growth was decreased.

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Investigation of the Condition of Acetic Acid Fermentation with High Concentration Ethanol Resistant Acetobacter sp. FM-10 (고농도 에탄올 내성균 Acetobacter sp. FM-10을 이용한 초산 발효조건 검토)

  • 박권삼;이명숙;목종수;장동석
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.5
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    • pp.845-848
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    • 1994
  • The fermenting conditions for acetic acid production with Acetobacter sp. FM-10 which could grow in the medium containing 10% ehtanol were investigated. Initial concentration of acetic acid in broth medium affected greatly to the fermentation speed. For example , the acetic acid production increased proportionally by the increasing of initial concentration was higher that 1.0%. When the cultivation was started with broth medium containing 5% ethanol, the additional adding ethanol during the fermentation was not significantly increased the acidity of the medium. The acidity of the medium containing 10% ethanol was reached to 8.3% after shaking than static cultivation by about 10 days with 150 rpm shaking speed. Acetic acid production with shaking cultivation was faster the static cultivation by abot 10 days under the same condition except shaking. In acetic acid fermentation with the batch style fermentor , the optimum fermentation condition was 700 rpm of agitation speed and 5L/min air flow rate in 3L culture medium .

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A Study on Cultural Conditions for Acetic Acid Production Employing Pear Juice (배를 이용한 식포의 발효조건에 관한 연구)

  • 오영준
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.4
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    • pp.377-380
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    • 1992
  • To produce economically important acid with pear juice, an acetic acid bacterium was selected from many isolated acetic acid bacteria. The alcohoic fermentation was conducted by inoculating pear juice with Saccharomyces cerevisiae ATCC 4124, and then the pear vinegar was prepared by batch cultivation in flaskes with the isolated Acetobacter sp. The optimum conditions for high yield of acetic acid were studied experimentally in the batch shake flask . For seed purposes the Acetobactor sp. was cultivated for 2 -days and transferred to the acid production medium . Optimum alcohol concentration, initial acidity and temperature for the acid production were 8.0% , 2.0% and 28$^{\circ}C$, respectively. Under the same conditions, the addition of yeast extract (1%) was observed to produce relatively high yield of acetic acid.

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Isolation and Cultural Properties of Acetic Acid Bacteria for Production of Onion Vinegar

  • Jang, Jae-Kweon;Choi, Young-Jin;Chung, Myong-Soo;Park, Hoon;Shim, Kun-Sub;Park, Young-Seo
    • Food Science and Biotechnology
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    • v.18 no.4
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    • pp.939-947
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    • 2009
  • In order to produce vinegar using onions, 12 acetic acid bacteria were screened from the juice of fallen peaches, and a strain showing the highest acetic acid productivity among them was selected and identified as Acetobacter tropicalis No. 22. The culture broth containing 2.5%(w/v) of initial sugar concentration showed maximum acetic production after 10 days of cultivation, and the acetic acid was produced at the highest rate and reached the maximum acidity after 2 to 6 days of cultivation when the residual sugar and the ethanol concentration were in the range of 1.6 to 2%(w/v) and 0.6 to 1.8%(v/v), respectively. Also optimum conditions for acetic acid production by response surface method using the fractional factorial design with 3 variables and 5 levels were involved with initial ethanol content of 4.67%(v/v), initial acidity of 0.03%, and initial glucose concentration of 2.35%(w/v) and predicted level of acetic acid production at these conditions was 3.77%.

Production of Acetic Acid from Cellulosic Biomass (섬유성 바이오매스를 이용한 Acetic Acid 생산)

  • 우창호;박준호;윤현희
    • KSBB Journal
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    • v.15 no.5
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    • pp.458-463
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    • 2000
  • Production of acetic acid from cellulosic biomass by Simultaneous Saccharification and Extractive Fermentation (SSEF) was investigated. The homoacetate organism used in this study was a strain of Clostridium thermoaceticum, ATCC # 49707. A batch operation of Simultaneous Saccharification and Fermentation(SSF) using ${\alpha}$-cellulose at pH 5.5 and 55$^{\circ}C$ yielded 40% conversion of cellulose to acetic acid, while a fed-batch SSF operation produced a maximum acetic acid concentration of 25 g/L, with 50% overall yield. In-situ extractive fermentation to reduce the end-product inhibition on both bacteria and enzyme was carried out. in a batch SSEF using 200 g/L IRA-400 resin, acetic acid concentration reached to 23.9 g/L and acetic acid yield and productivity were observed to be 48% and 0.20 g/L-hr, respectively.

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Effect of Ethanol on the Production of Cellulose and Acetic Acid by Gluconacetobacter persimmonensis KJ145 (Gluconacetobacter persimmonensis KJ145를 이용한 Bacterial Cellulose 및 초산발효에 미치는 Ethanol의 영향)

  • 이오석;장세영;정용진
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.2
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    • pp.181-184
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    • 2003
  • We investigated the effect of ethanol on the production of cellulose and acetic acid fermentation by Gluconacetobacter persimmonensis KJ145. Results showed that bacterial cellulose productivity was highest when 2% ethyl alcohol was added to apple-juice medium. For acetic acid production, 7% ethyl alcohol was needed. Optimal concentration of ethyl alcohol was 5% for simultaneous production of bacterial cellulose and acetic acid. For simultaneous production of bacterial cellulose and acetic acid, optimal nitrogen source and optimal concentration were corn steep liquor and 15% (w/v), respectively Optimal culture time for simultaneous production of bacterial cellulose and acetic acid was 14 days. At the optimal condition, Cluconacetobacter persimmonenis KJ145 produced 7.55 g/L of bacterial cellulose (dry weight).

Study on the Production of Organic Acid by Fermentation with Mixed Culture of Lactobacillus acidophilus KCCM 32820 and Propionibacterium freudenreichii KCCM 31227 in Whey Broth (유청배지에 Lactobacillus acidophilus KCCM 32820과 Propionibacterium freudenreichii KCCM 31227로 혼합배양시 유기산 생성에 관한 연구)

  • Lee, Jeong-Hoon
    • Culinary science and hospitality research
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    • v.13 no.1 s.32
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    • pp.55-61
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    • 2007
  • This study was carried out to evaluate the growth characteristics of Lactobacillus acidophilus KCCM 32820 and Propionibacterium freudenreichii KCCM 31227 and the production of propionic and acetic acids in 5% and 10% whey broth by mixed culture of L. acidophilus KCCM 32820 and P. freudenreichii KCCM 31227. Exponential phase of L. acidophilus KCCM 32820 was in the range of $6\sim12$ hrs and P. freudenreichii KCCM 31227 was in the range of $36\sim108$ hrs. In the mixed culture, production of propionic acid was shown to be greater value in the 10% whey broth than in the 5% whey broth and to be greater value in the low temperature for a long time than in sterilization by autoclave. Maximum production of propionic acid was 8.88 mg/mL in the 10% whey broth fermented at 120hrs. Production of acetic acid was revealed to be greater value in the 10% whey broth than in the 5% whey broth. The production quantity ratio of propionic acid to acetic acid was shown between $2:1\sim3:1$ during the fermentation process.

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