• Journal of Microbiology and Biotechnology
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• 제20권6호
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• pp.995-1000
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• 2010

A novel deoC gene was identified from Paenibacillus sp. EA001 isolated from soil. The gene had an open reading frame (ORF) of 663 base pairs encoding a protein of 220 amino acids with a molecular mass of 24.5 kDa. The amino acid sequence was 79% identical to that of deoxyribose 5-phosphate aldolase (DERA) from Geobacillus sp. Y412MC10. The deoC gene encoding DERA was cloned into an expression vector and the protein was expressed in Escherichia coli. The recombinant DERA was purified using Ni-NTA affinity chromatography and then characterized. The optimum temperature and pH of the enzyme were $50^{\circ}C$ and 6.0, respectively. The specific activity for the substrate deoxyribose 5-phosphate (DR5P) was $62\;{\mu}mol/min/mg$. The $K_m$ value for DR5P was determined to be 145 mM with the $k_{cat}$ value of $3.2{\times}10^2/s$ from Lineweaver-Burk plots. The EA001 DERA showed stability toward a high concentration of acetaldehyde (100 mM).

• Korean Chemical Engineering Research
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• 제47권2호
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• pp.248-257
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• 2009

본 연구에서는 배출량산정모델과 다중매질모델을 활용하여 환경오염물질의 노출도를 예측하였으며 위해도를 평가하였다. 연구대상 화학물질로써 8종(아세트알데히드(acetaldehyde), 아크릴로니트릴(acrylonitrile), 아닐린(aniline), 벤젠(benzene), 사염화탄소(carbon tetrachloride), 디클로로메탄(dichloromethane), 포름알데히드(formaldehyde), 염화비닐(vinyl chloride))의 물질을 선택하였으며, 대상지역은 공단지역을 포함하는 도심 지역을 선택하였다. 배출량은 지리지형정보를 활용하여 점배출원과 비점배출원을 동시에 고려하여 산정하였으며, 다중매질모델은 지역적 특성을 반영할 수 있는 모델을 선택하였다. 유해성 자료는 미국 환경청의 IRIS(Integrated Risk Information System) 유해성 데이터베이스를 활용하였다. 모델링 자료와 유해성 자료를 이용하여 위해성을 평가한 결과, 물질별로 위해도가 높은 지역을 발견할 수 있었으며 우선적으로 관리해야 할 물질을 선별할 수 있었다.

• 한국환경과학회지
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• 제20권8호
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• pp.953-962
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• 2011

The covered stream of cities are considered an odor source. Also, the public do not want a wastewater treatment plant(WWTP) near their properties due to the emission of odor emanating from such sources, although they play an important role in urban development. The purpose of this study is to analyze the pattern distribution of the odorous compounds from the Nambu WWTP and Youngho stream in Busan. odor sampled four times were analyzed by instrumental analysis method and indirect olfactory method. The kinds of offensive odorous compounds examined are acetaldehyde, propion aldehyde, hydrogen sulfide, methyl mercaptan, dimethyl sulfide and ammonia. Also, Concentration of air pollutants has been calculated by ISCST3 models. At the result of this study, The Nambu WWTP releases sulfur compounds. And the major odorous were hydrogen sulfide (1,475 ppb) and acetaldehyde (95 ppb) at Youngho stream. The stink which residents feel will point out the Nambu WWTP mainly if the odor is removed with the improvement of a Youngho stream. Accordingly, we should pay more attention to appropriate components to processes in odor reducing plan at Nambu WWTP.

• 대한건축학회논문집:구조계
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• 제35권10호
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• pp.217-223
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• 2019

The purpose of this study was to measure the indoor air quality of small-scale apartments and then proposed a method to improve the indoor air quality of the small-scale apartments by comparing with the result of indoor air quality in the Clean-Healthy House. In case of formaldehyde, a highly volatile organic compound, the concentrations of indoor measured both in small-scale apartments and the Clean Healthy houses did not exceed- the guideline of Korea. The guideline of Korea for acetaldehyde was not available, so the guidelines of Japan and the World Health Organization were used to measure the concentration of acetaldehyde. The study results demonstrated that three out of seven small-scale apartments investigated exceeded the guideline of Japan; and the preliminary research on the Clean-Health Houses was low at about 30-60% of the guideline of Japan. The result of the VOCs measurement showed that toluene, ethylbenzene, and styrene in some small-scale apartments exceeded the guideline of Korea. However, the Clean-Healthy Houses in the previous studies did not exceed the guideline of Korea with regard to all the pollutants. Therefore, this study suggests the application of the construction standards of the Clean-Healthy house in order to improve the indoor air quality of small-scale apartments. In addition, the current study anticipates that improvement of the indoor air quality in the residential environments should be universally applied, not limited to the type and size of the residence.

• 한국식품저장유통학회지
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• 제30권4호
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• pp.617-629
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• 2023

Reactive oxygen species are the byproducts of metabolic processes in the body, However, excessive amount may cause side effects such as cancer. Therefore, to reduce the production of these species, but their long-term administration at high doses may induce side effects. Hence, natural materials with antioxidant activities are attracting attention. Two of these natural materials are soybean sprouts and Hovenia dulcis Thunb. fruits, but few studies have evaluated the effects of their combination. Thus, we prepared a soybean sprout extract containing 1.5% H. dulcis Thunb. fruit concentrate (BHM) to develop a functional food material derived from natural products and then confirmed its physicochemical properties and physiological activity. Among the organic acids detected in BHM, malic acid exhibited the highest content of 1,451.03 ppm, and the main free sugars were glucose (645.48 ppm) and fructose (738.11 ppm). Taurine was the most abundant free amino acid at a concentration of 11.95 ppm, followed by those of arginine (10.97 ppm) and glutamic acid (10.16 ppm). Analyses of the mineral components revealed large amounts of Zn and Fe in BHM, and the respective total polyphenol and flavonoid contents in BHM were 957.16 and 601.93 ppm. The DPPH radical and H₂O₂ scavenging activities and reducing power indicated excellent antioxidant efficacy compared to the positive controls. Furthermore, blood alcohol and acetaldehyde concentrations were measured to confirm the hangover-relieving effects of BHM, with both significantly decreased (p<0.05). BHM displays potential for development as a functional food, and the results of this study may be used as basic data in further research.

• 한국산학기술학회논문지
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• 제18권2호
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• pp.218-225
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• 2017

본 연구에서는 알코올을 섭취한 흰쥐에서 알코올 대사와 혈액가스 불균형에 대한 꾸지뽕 나무 뿌리 추출물 (이하 CTE)의 효과를 확인하고자 하였다. 알코올을 10 ml/kg의 농도로 흰쥐에게 경구 투여하였고, 섭취 30분 후 CTE를 500 mg/kg 및 750 mg/kg의 농도로 투여하였다. 혈액은 알코올 섭취 1시간, 3시간, 5시간 후 꼬리 정맥을 통하여 채취하였다. 양성 대조군은 시판되고 있는 숙취해소 음료 (컨디션)를 사용하였다. 약물 처리는 모두 단회 경구 투여로 진행되었다. CTE의 섭취는 흰쥐의 혈액과 간 조직 내 알코올 대사 효소인 알코올 분해효소 (alcohol dehydrogenase)와 아세트알데히드 분해효소(acetaldehyde dehydrogenase)를 증가 또는 유지시킴으로써 혈중 알코올 농도를 감소시켰다. 또한, CTE는 알코올섭취에 의한 혈액가스 (pH, $CO_2$, $HCO_3{^-}$, lactic acid)의 불균형을 회복시켰다. 결론적으로, CTE는 현재 시판되고 있는 숙취해소음료 보다 우수한 숙취 해소 효과를 나타내었다. 따라서 본 연구자는 CTE를 숙취 해소 및 예방을 위한 천연물 유래의 안전하고 새로운 물질로써 제시하는 바이다.

• 한국축산시설환경학회지
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• 제11권3로
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• pp.189-196
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• 2005

본 연구는 국내에 설치되어 있는 액비저장조의 운영실태를 조사하고, 액비저장조에서 발생되는 악취물질을 구명하고자 수행하였다. 1. 국내에 200톤 규모의 액비저장조를 설치한 60 농가를 대상으로 한 본 조사에서 약 $93\%$가 액비저장조를 제대로 가동을 하고 있었으며 이중 $57\%$가 액비제조시 폭기 처리하고 있었다. 2. 조사 농가 중 년간 액비의 활용 횟수는 2회가 $50\%$로 나타났고 액비의 부숙 효과를 높이고 악취를 저감시키기 위한 목적으로 $64\%$가 미생물제제를 첨가하고 있었다. 반면에 액비 이용시 인력 장비의 비용 및 악취물질 발생 저감을 위한 첨가제 비용이 $43\%$로 조사되어 액비이용 시 문제점으로 지적되었다. 3. 액비 제조 형태별 악취물질 발현양상으로 호기 제조시 액비저장조내에서는 iso-valeric acid가 0.012에서 0.07ppm, Propionic acid가 $0.17\~2.85ppm$의 범위로 검지되었으며 혐기 제조시에는 n-Butyric acid가 1.5에서 2.3ppm, n-valeric acid가 $1.3\~1.8ppm$, acetaldehyde가 0.8에서 2.1ppm로 검지되어 호기 제조방법과 혐기 제조방법 모두 휘발성 지방산의 농도는 악취방지법의 규제농도 이상이었다. 부지경계선에서의 악취물질을 보면 호기시에는 미검출로 나타났고 혐기 제조시에는 Acetaldehyde가 $0.4\~0.9ppm$ 수준으로 검지되어 악취방지법의 규제농도를 초과였다.

• 한국식품저장유통학회지
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• 제2권2호
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• pp.225-232
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• 1995

In order to study the effect of modified atmosphere packaging(M.A.P.) and controlled atmosphere(C. A.) storage on keeping freshness of mushrooms (Agricus-bisporus). Mushrooms was packaged with polyethylene(P.E.) film (40${\mu}{\textrm}{m}$, 60${\mu}{\textrm}{m}$, 80${\mu}{\textrm}{m}$) and C.A. conditions(CO2 concentration of 2%, O2 concentration of 2%) and storage at 0$\pm$1$^{\circ}C$, RH(relative hummidity) 92$\pm$1%. Gas composition in film was changed rapidly at early of storage, but it kept a constant level after 14 days of storage, and then kept at the level of 6~9% CO2 and 2~5% O2. Weight loss was 5% in non-packed mushrooms after 3 days of storage, but P.E. film, CO2 treated, C.A. conditions were 5% after 21 days of storage. Flesh firmness of the mushrooms was continuously decreased throughout storage period and the lowest of flesh firmness changed was 80${\mu}{\textrm}{m}$-P.E. film packing. Discoloration of the piteous of mushrooms appears to be the most Important factors to determine its marketability, L value of it appears to be of the high values at P.E. film packing and C.A. conditions during period. Large amount of ethanol and acetaldehyde were produced from the 7 days during storage, large contents of mannitol and trehalose were at the 14 days and 7 days during storage.

• 유통과학연구
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• 제18권9호
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• pp.67-75
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• 2020

Purpose: The purpose of this study is to enhance sales promotion efficiency for using solid refuse fuel facilities. Renewable energy technology using Solid Refuse Fuel (SRF) is an economic efficiency technology that recovers waste by burning various wastes. A survey on the pollutants discharged from the solid fuels facilities was investigated so that the SRF facilities could be expanded, distributed and reflected in the policy. Research design, data, and methodology: In this study, 9 business sites using SRF and Bio-SRF as main raw materials were investigated for 2 years. The characteristics of target business sites such as the type of fuel used, combustion method, combustion temperature, daily fuel consumption and environmental prevention facilities were studied. Results: The average pollution & ammonia concentration of Bio-SRF facilities was found to be 88.15% higher than that of SRF facilities. But the average acetaldehyde concentration of SRF facilities was found to be 88.15% higher than that of Bio-SRF facilities. Conclusions: The main issue is how much electric power generation using combustible materials affects air pollution. The waste recycling law provides the standard value according to the fuel property, but there is a considerable gap with the mixed fuel. Therefore, for efficient utilization of facilities using solid fuel products, additional research is needed to improve the distribution structure of exhaust pollutants is needed.

• 웰빙융합연구
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• 제4권2호
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• pp.35-41
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• 2021

Purpose: In this study, volatile organic compounds(VOCs) and aldehydes generated from roadside vehicles and other pollutants were measured and analyzed. Research design, data and methodology: As a result of measuring and analyzing three areas near the roadside, Vinyl chloride 0.00 ~ 0.02 ppb, Benzene 2.87 ~ 5.01 ppb. Toluene 4.51 ~ 8.62 ppb, Styrene 0.00 ~ 0.34 ppb, Formaldehyde 8.45 ~ 17.12 ug/m³, Acetaldehyde 7.01 ~ 17.64 ug/m³ were detected. When comparing the analysis results and the 6-month average concentration of the hazardous air monitoring network, the analysis results were about 26 times higher for Benzene, about 5 times for Toluene, and about 3.75 times for Styrene. In the case of vinyl chloride, it was confirmed that it was about 20 times lower than that of the hazardous atmosphere monitoring network. Results: Therefore, it is necessary to reexamine the installation location of the measurement network because people are exposed to pollutants on the actual roadside. It is judged that it is right to build a measurement network that is practically helpful to people by increasing the measurement items in the measurement network.