• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.38 no.4
• /
• pp.231-239
• /
• 2012

Objectives: This study sought to provide guidelines in order to decrease the incidence of nerve injury during mandibular ramus bone harvesting, and to improve understanding of the anatomical structure of the inferior alveolar canal (IAC) to include its distance from the exterior buccal cortex. Materials and Methods: In January and February 2009, 20 patients who visited the Wonkwang University Department of Oral and Maxillofacial Surgery reporting various conditions underwent cone beam computed tomography and were included in this study. Patients with missing left or right mandibular first molars or incisors, or who had jaw fracture or bone pathologies, were excluded. The reference point (R point) was defined as the point where the occlusal plane reached the anterior ramus of the mandible. The position of the IAC in relation to the R point, the buccal bone width (BW), the alveolar crest distance (ACD), the distance from the alveolar crest to the occlusal plane (COD), and the distance from the IAC to the sagittal plane (CS) were determined using proprietary image analysis software which produced cross-sectional coronal and axial images. Results: The distance medially from the R point to the IAC along the axial plane was $6.19{\pm}1.21mm$. The HD from the R point, posteriorly to IAC, in the lateral view was $13.07{\pm}2.45mm$, the VD from the R point was $14.24{\pm}2.41mm$, and the ND from the R point was $10.12{\pm}1.76mm$. The pathway of the IAC was positioned almost in a straight line along a sagittal plane within $0.56{\pm}0.70mm$. The distance from the buccal bone surface to the IAC increased anteriorly from the R point. Conclusion: Marking osteotomy lines in the retromolar area in procedures involving bone harvesting should be discouraged due to the risk of damage to IAC structures. Our measurements indicated that the area from the R point in the ramus of the mandible to 10 mm anterior can be safely harvested for bone grafting purposes.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.33 no.4
• /
• pp.323-328
• /
• 1988

The hop alpha-acid content prediction model developed with meteorological elements in Hoeongseong was Y=28.369-0.003X$_1$＋1.558X$_2$-1.953X$_3$-0.335X$_4$-0.003X$\sub$5/-0.119X$\sub$6/, with MSEp of 0.004, Rp$^2$ of 0.9987, Rap$_2$ of 0.9949 and Cp of 7.00. The total sunshine hours (X$_1$), the maximum temperature (X$_3$) and the total precipitation (X$\sub$5/) at flowering stage. the maximum temperature at flower bud differentiation stage (X$_4$) and the maximum temperature at cone ripening stage (X$\sub$6/) influenced on hop alpha .acid content as decrement weather elements. The maximum temperature at cone development stage(X$_2$) effected on ${\alpha}$-acid content as increment weather element.

• The Journal of Korean Obstetrics and Gynecology
• /
• v.19 no.1
• /
• pp.31-46
• /
• 2006

Purpose : The Purpose of this research was to investigate the effects of antithromb otic activities of Honghwadangguisan(HDS) Methods : Measured the effect which was given to blood flow rate through the regular volume of glass tube after the blood was diluted five times with ACD soulution. Antithrombotic effect was calculated as a percentage of the experimental animal figure protected from the paralysis of hind legs or death of the mouse that is caused from the administration of platelet aggregation regent. Each of the groups consisted in 8 mice, was divided into Normal, Control, and HBS. All of these 3 group were supplied a saline solution and after an hour the control group brought the dextran extravasated blood. Also the HDS group was dosed to the experimental mice with Oral Zonde one day before the experiment. After that, the mice were abstained from food. And then we gave a measured amount of it before an hour. Finally, it gave rise to dextran extravasated blood as well as the Control group. Results : The results were obtained as follows. In vitro, HDS inhibited platelet aggregation induced by ADP and epinephrine significantly as compared with the control group. HDS showed fibrinolytic activity insignificantly as compared with the control group. HDS reduced blood flow rate in significantly as compared with the control group. In vivo HDS inhibited pulmonary embolism induced by collagen and epinephrine (inhibitive rate 50%). HDS increased number of platelet, fibrinogen amount and shortened prothrombin time, activated partial thromboplastin time significantly but reduced blood flow rate insignificantly as compared with the control group in thrombus model induced by dextran. Conclusion : HDS is effective antithrombotic activity from experimental result.

• Maxillofacial Plastic and Reconstructive Surgery
• /
• v.31 no.5
• /
• pp.386-393
• /
• 2009

Objectives: This study was performed to evaluate course of the inferior alveolar canal in the mandibular ramus and to find safety zone when ramal bone is harvested. Patients and Methods: From January, 2009 to February, 2009, the 20 patients who visited in the Department of Oral and Maxillofacial Surgery, Sanbon Dental Hospital. Wonkwang University and the Conebeam CT was taken of various chief complaints, were selected. The patients who had left and right mandibular first molar and incisor missing, jaw fracture and bone pathology were excluded. The R point was defined as the point which occlusal plane was crossed to the mandibular anterior ramus(external oblique ridge). In the cross-sectional coronal and axial views, the inferior alveolar canal position to the R point, buccal bone width(BW), alveolar crest distance(ACD), distance from alveolar crest to occlusal plane(COD) and inferior alveolar canal to sagittal plane(CS) were measured and horizontal distance(HD), vertical distance(VD) and nearest distance(ND) were measured. Results: The inferior alveolar canal is located $6.19{\pm}1.21\;mm$ from the R point. Horizontal distance from the R point were $13.07{\pm}2.45\;mm$, vertical distance from the R point were $14.24{\pm}2.41\;mm$ and nearest distance from the R point were $10.12{\pm}1.76\;mm$. The course of the inferior alveolar canal was positioned within $0.61{\pm}0.68\;mm$. The distance from external buccal bone to the inferior alveolar canal was increased from the R point anteriorly. Conclusions: It is considered that the mandibular ramus from the R point to 10 mm anteriorly can be harvested safely at ramal bone grafting.

• The Journal of Korean Obstetrics and Gynecology
• /
• v.18 no.3
• /
• pp.110-126
• /
• 2005

Purpose : The Purpose of this research was to investigate the effects of antithrombotic activities of Wuslsan (WSS). Methods : Measure the effect which was given to blood flow rate through the regular volume of glass tube after the blood was diluted five times with ACD soulution. Antithrombotic effect was calculated as a percentage of the experimental animal figure protected from the paralysis of hind legs or death of the mouse that is caused from the administration of platelet aggregation regent. Being classified one group of eight mice, each of them was divided into Normal, Control, and WSS. The normal group supplied a saline solution and the control group brought the dextran extravasated blood after an hour of administering the saline solution. Also WSS was dissolved in 2ml saline solution and then we dosed it to the experimental mice with Oral Zonde one day before the experiment. After that, the mice were abstained from food. And then we gave a measured amount of it before an hour. Finally, it gave rise to dextran extravasated blood in the same way as the Control group. Results : The results were obtained as follows. WSS inhibited platelet aggregation induced by ADP and epinephrine significantly as compared with the control group. WSS showed fibrinolytic activity insignificantly as compared with the control group. WSS increased blood flow rate significantly as compared with the control group in vitro. WSS inhibited pulmonary embolism induced by collagen and Epinephrine(inhibitive rate is 37.5%). WSS increased number of platelet and fibrinogen amount significantly, and shortened PT and APTT as compared with the control group in thrombus model induced by dextran. Conclusion : WSS is effective antithrombotic activity from experimental result.

• The Journal of Korean Obstetrics and Gynecology
• /
• v.18 no.1
• /
• pp.1-14
• /
• 2005

This study was performed to evaluate antithrombotic activities of Saegeumsan (瑞金散, SGS) which has effects of activating blood, removing thrombus. This study is designed to measure the effect which was given to blood flow rate through the regular volume of glass tube after the blood was diluted five times with ACD solution. Antithrombotic effect was calculated as a percentage of the experimental animal figure protected from the paralysis of hind legs or death of the mouse that is caused from the administration of platelet aggregation regent. We standardized the time when the experimental animals were incapable of functioning the hind legs more than 20 minutes or maintained trembling. Being classified one group of eight mice, each of them was divided into Normal, Control, and SGS. The normal group supplied a saline solution and the control group brought the dextran extravasated blood after an hour of administering the saline solution. Also, SGS was dissolved in $2m{\ell}$ saline solution and then we dosed it to the experimental mice with Oral Zonde one day before the experiment. After that, the mice were abstained from food. And then we gave a measured amount of it before an hour. Finally, it gave rise to dextran extravasated blood in the same way as the Control group. The results were obtained as follows, SGS significantly inhibited platelet aggregation induced by ADP and epinephrine when analyzed by the Sigmoid $E_{max}$ model in WinNonlin. $EC_{50}$ values of SGS were 4.61 mg/ml and 12.41 mg/ml for ADP and epinephrine respectively. SGS showed fibrinolytic activity insignificantly as compared with the control group. SGS increased blood flow rate significantly as compared with the control group in vitro. SGS inhibited pulmonary embolism induced by collagen and epinephrine(inhibitive rate is 37.5 %). SGS increased number of platelet and fibrinogen amount significantly, and shortened PT and APTT as compared with the control group in thrombus model induced by dextran. According to, SGS is effective antithrombotic activity from experimental result.

• Childhood Kidney Diseases
• /
• v.9 no.1
• /
• pp.76-82
• /
• 2005

Purpose : Regional anticoagulation with trisodium citrate for continuous renal replacement therapy(CRRT) is an effective and safe method, with lower bleeding risk. However it is not widely used because of complex current protocols used to prevent anticipated metabolic derangements. We evaluated simplified regional anticoagulation protocols with ACD-A(R) solution and commercially available calcium-containing dialysis solution. Methods : The medical records of twenty-eight patients who underwent CRRT were reviewed. Hemofilter life span according to the anticoagulation method used was compared, and laboratory findings at Pre- and 48 hours post-CRRT initiation were compared in the citrate-based CRRT group. Results : Of the twenty-eight Patients, five patients underwent citrate-based CRRT Hemofilter life span was 1.60 $\pm$ 0.72 days, showing no significant differences with the hemofilter life span in the heparin based and LMWH based CRRT group. No patients experienced hemorrhagic complications. PT, aPTT, sodium, t$CO_{2}$, iCa levels showed no difference in pre- and post-CRRT. Total calcium levels were increased. At the recommended postfilter iCa level, j.e., 0.25-0.39 mmol/L, all five patients needed increased amount of citrate infusion, and Ca infusion requirement was decreased. Conclusion : Simplified regional citrate anticoagulation with calcium-containing dialysate is an effective and safe method, and is not associated with increased hemofilter clotting. However, increased postfilter iCa level is recommended.

• The Korean Journal of Nuclear Medicine
• /
• v.24 no.1
• /
• pp.37-48
• /
• 1990

Detection of deep-seated abscesses is sometimes difficult with ultrasonogrpahy or computed tome graphy alone. Indium-111-labeled leukocyte has widely used in the localization of abscesses after introduction by Segal and Thakur in 1976. But there are some difficulties in using indium-111-oxine in our country because of hardness to get the radiopharmaceutical timely and long time for labeling leukocytes. So we peformed the indium-111-labeled leukocyte scan for establishment of the labeling procedure and clinical application. We labeled the mixed leukocytes from 36 ml of patient's blood using 4 ml of ACD solution, 7 ml of 6% hydroxyethyl starch solution $(HESPAN^{(R)})$, 1 mCi of indium-111 oxine, 5 ml of normal saline and centrifuge. It took about 2 hours for the preparation of radiolabeled leukocytes and attention for contamination was needed. The average injected dose of labeled mixed leukocytes was 465 uCi. The average number of injected leukocytes was $2.5\times10^8$ and the labeling ratio was $57{\pm}13%$ (Table 2, Fig. 5). These number and ratio were sufficient for the localization of abscess. About twenty per cent of indium was labeled to red blood cells and platelets (Fig. 6) and the half-life of injected radiolabeled leukocytes was 8.3 hours. Scan was performed in 9 patients who were suspected to have abscesses clinically or radiologically. Three patients were positive, in one patient who had abscess close to lower lumbar vertebrae was surgically drained and another 2 positive cases did not show abscess clearly on computed tomography, so only antibiotics were administrated and treated successfully. The negative 6 patients were improved without specific treatment. In conclusion, the use of indium-111 oxine labeled leukocytes for localization of abscesses were very specific and helpful in the decision of treatment considering its relatively simple labeling method, and could be easily performed providing timely supply of the radiopharmaceutical.

• The Korean Journal of Nuclear Medicine
• /
• v.14 no.2
• /
• pp.1-9
• /
• 1980

혈액질환(血液疾患), 특히 용혈성빈혈(溶血性貧血)을 수반(隨伴)한 경우(境遇)에 적혈구(赤血球)의 생성(生成), 파괴과정(破壞過程)을 정확히 파악하는 것은 빈혈(貧血)의 발생기전(發生機轉) 및 병인(病因), 치료(治療), 예후(豫後) 결정(決定)에 매우 중요(重要)하다. 적혈수명측정법(赤血壽命測定法)은 최근(最近) 방사성(放射性)동위원소(同位元素)를 이용(利用)한 방법(方法)이 소개된 이래 널리 시행(施行)되어 왔다. 그러나 그 방법(方法) 및 결과(結果) 해석(解釋)에 표준화(標準化)가 되어 있지 않았던 중 1971년 ICSH (International Committee for Standardization in Hematology)에서 expert panel을 갖고 ICSH 추천 방법(方法)을 발표(發表)하였고, 본지(本誌)에서도 그 내용(內容)을 게재(揭載)한 바 있다. 1980년 ICSH는 전문기관 및 전문가의 협조(協調)를 얻어 다시 expert panal을 갖은 후 1971년에 추진한 적혈구수명측정법(赤血球壽命測定法)의 일부(一部)를 수정(修正)하여 ICSH의 표준방법(標準方法)으로 발표(發表)하였다. 개정(改正)된 표준방법(標準方法)과 1971년 ICSH추친 방법(方法)과의 차이(差異)는 다음과 같다. $^{51}Cr$표지방법중(標識方法中) 참고방법(參考方法)(Reference method)인 ACD법(法)에 수정(修正)을 가하여, ACD solution 구성성분(構成成分)이 차이(差異)가 있으며, 표지(標識)$^{51}Cr$의 양(量)을 체중당(體重當) $1.5{\mu}Ci$에서 $0.5{\mu}Ci$로 제한(制限)시켰다. 투여방법(投與方法)에 대한 언급 특히 투여하는 표지적혈구(標識赤血球)의 용적을 정확하게 측정 하기 위한 방법 4가지를 추가하였고, 검체준비(檢體準備) 과정중(過程中)의 pipet error를 방지(防止)하기위해 일정(一定)한 형태의 pipet을 사용(使用)하며, 1ml의 tuterculin syringe는 사용(使用)하지 않기로 하였다. 또한 결과분석시(結果分析時) 혈구용적(血球容積)의 항정성(恒定性)을 위해 Sodium pertectnetate($^{99m}Tc$)를 이용(利用)해 혈구용적(血球容積)을 반복(反復)해 측정(測定)하도록 하였으며 이때 사용(使用)하는 방사성동위원소(放射性同位元素)는 $^{32}P$ 대신 $^{99m}Tc$로 하였다. 결과해석시(結果解釋時) IgG 항체(抗體) 또는 IgM 항체(抗體)에 따른 차이점(差異點)에 대한 고려가 추가(追加)되었다. ICSH와 국제혈액학회(國際血液學會)에서 수정(修正)된 ICSH 표준방법(標準方法)에 의한 적혈수명측정법(赤血壽命測定法)을 널리 소개(紹介)하여 결과(結果)의 표준화(標準化)를 기하고자 연관잡지(聯관(關)雜誌)에 게재(揭載)할 것을 요청(要請)하였기에 전문(全文)을 본지(本誌)에 소개(紹介)하고자 한다.

• Fisheries and Aquatic Sciences
• /
• v.26 no.9
• /
• pp.558-568
• /
• 2023

Vibrio vulnificus is an aquatic bacterium causing septicemia and wound infection in humans. To understand this pathogen at the genomic level, it was performed whole genome sequencing of a cefoxitin-resistant strain, V. vulnificus 1908-10 possessing virulence-related genes (vvhA, viuB, and vcgC) isolated from Gadeok island coastal seawater in South Korea. The genome of V. vulnificus 1908-10 consisted of two circular contigs and no plasmid. The total genome size was estimated to be 5,018,425 bp with a guanine-cytosine (GC) content of 46.9%. We found 119 tRNA and 34 rRNA genes respectively in the genome, along with 4,352 predicted protein sequences. Virulence factor (VF) analysis further revealed that V. vulnificus 1908-10 possess various virulence genes in classes of adherence, antiphagocytosis, chemotaxis and motility, iron uptake, quorum sensing, secretion system, and toxin. In the comparison of the presence/absence of virulence genes, V. vulnificus 1908-10 had fur, hlyU, luxS, ompU, pilA, pilF, rtxA, rtxC, and vvhA. Of the 30 V. vulnificus comparative strains, 80% of the C-genotype strains have all of these genes, whereas 40% of the E-genotype strains have all of them. In particular, pilA were identified in 80% of the C-type strains and 40% of the E-type strains, showing more difference than other genes. Therefore, V. vulnificus 1908-10 had similar VF characteristics to those of type C strains. Multifunctional-autoprocessing repeats-in-toxin (MARTX) toxin of V. vulnificus 1908-10 contained 8 A-type repeats (GXXGXXXXXG), 25 B.1-type repeats (TXVGXGXX), 18 B2-type repeats (GGXGXDXXX), and 7 C-type repeats (GGXGXDXXX). The National Center for Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLAST) showed that the RtxA protein of V. vulnificus 1908-10 had the effector domain in the order of cross-liking domain (ACD)-C58_PaToxP-like domain- α/β hydrolase-C58_PaToxP-like domain.