• Title/Summary/Keyword: accumulated maximum level

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O1igosaccharide Formation and Production of Transfructosylase and Transglucosylase by Aureobasidium pullulans (Aureobasidium pullulans가 생산하는 과당 및 포도당 전이효소에 의한 올리고당류의 생산)

  • 윤종원;윤태경한성범송승구
    • KSBB Journal
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    • v.9 no.2
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    • pp.133-139
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    • 1994
  • Oligosaccharide formation and the production of transfructosylase and transglucosylase by Aureobasidium pullulans were studied in sucrose or maltose media, respectively. The initial uptake rates of substrate in sucrose-rich media were faster than that in maltose-rich media, also most parts of oligosaccharides formed and other monosaccharides released were utilized progressively as substrate during the cultivation periods. However, when the initial amount of sucrose was raised to $100g/\ell$, high concentration of monosaccharides were liberated, consequently high-level fructose was accumulated unused during fermentation. The biggest molecule of oligosaccharide synthesized was hexasaccharide in all cultivation media examined, of which the organism could not utilize isomalto-oligosaccharide of DP6 synthesized in a maltose-rich medium. The maximum amount of oligosaccharides produced was $58g/\ell$ when $100g/\ell$ of sucrose and $5g/\ell$ of maltose were used as initial substrate. From the early stage of growth both fructooligosaccharides and isomalto-oligosaccharides were synthesized and progressively utilized as substrates during the fermentation. Based on the experimental results, it was suggested that maltose could induce both transfructosylase and transg1ucosylase, whereas sucrose was unable to slimulate transglucosylase formation.

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Ovarian and Fat Body Yolk Protein Synthesis in Culex piplens pallens (홍모기(Culex pipiens pallens) 지방체와 난소에서의 난황단백질합성에 관한 연구)

  • 이승훈;박영민;성기창
    • The Korean Journal of Zoology
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    • v.36 no.3
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    • pp.416-424
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    • 1993
  • Ovarian Yolk protein (YP2) synthesis has been investigated in mosquito, Culex pipiens pallens. Yolk protein amount which was syntheized in fat body, accumulated into ovary were analyzed by Rocket immunoelectrophoresis and in vitro organ culture. The result was that yolk protein synthesis began to occur at 6hrs after blood meal, reached at maximum level by 24hrs, and was completed within 48hrs. Yolk protein accmulation into the ovary began to start at 6hrs and coutinued for up to 60hrs after blood meal. Extract from 0, 24, 48, 72hrs ovaries after blood meal were analyzed by electrophoresis and Western blotting. The result was that 24hrs ovary contain one yolk protein(YP1), and 48, 72hrs ovaries contain two kinds of yolk proteins(YPl and YP2). When 48hr ovaries and fat bodies were incubated in $^3$H-leucine contained medium, protein synthesis was not occurred in fat body, but ovary synthesized much protein contained yolk protein (YP2). The result of crossed immunoelectrophoresis represented the same immunity between YPl and YP2. The present data suggest that ovary synthesize yolk protein(YP2) in mosquito, Culex pipiens pallens.

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Physicochemical Characteristics and Formation Environments of the Ujeon Coastal Dune Depositsin Jeungdo (증도 우전 해안사구 퇴적층의 물리화학적 특성과 형성환경)

  • Oh, Jeong-Sik
    • Journal of The Geomorphological Association of Korea
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    • v.25 no.2
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    • pp.43-61
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    • 2018
  • Heterogeneous sedimentary deposits with different soil colors and various degree of hardness are exposed in its foredune and tidal zone due to the effects of recently accelerated coastal erosion along the Ujeon Coast in Jeung-do, Shinan-gun. This study was conducted on the assumption that these sedimentary deposits were developed in different timing and environments. Thus, we can infer the geomorphic development processes of the area based on evidences like the physicochemical characteristics of each sedimentary layer. Several analysis of these sedimentary depositssuch as grain size analysis, X-ray Fluorescence Measurement (XRF), and Loss on ignition (LOI) were performed on central (Ujeon A) and southern (Ujeon B) parts of the Ujeon Coast. I found that the foredune sedimentary deposits have four stages of geomorphic development processes. In the initial stage of development, during the peak of the Last Interglacial Period (MIS 5e), basal deposits were accumulated in the low-energy environment of subtidal zones. In the second stage, during the Last Glacial Period (MIS 4~MIS 2), eolian sedimentary layers were developed by terrestrial aeolian processes by which fine materials were transported from the Yellow Sea which became a dry land exposed by lowered sea level. In the third stage, various mechanism existed for the formation of each sedimentary layer. In the region of Ujeon A, sedimentary layers were developed in the littoral zone environment dominated by marine processes during the maximum phase of transgression in the Holocene. Meanwhile, the region of Ujeon B began to form eolian sedimentary layers during MIS 2. In the last stage, thick coastal dune deposits, covered all over the Ujeon Coast. During the late Holocene (0.7~0.6 ka), terrestrial processes kept dominating the region, developing typical eolian sedimentary layers.

Skin Region Detection Using Histogram Approximation Based Mean Shift Algorithm (Mean Shift 알고리즘 기반의 히스토그램 근사화를 이용한 피부 영역 검출)

  • Byun, Ki-Won;Joo, Jae-Heum;Nam, Ki-Gon
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.48 no.4
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    • pp.21-29
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    • 2011
  • At existing skin detection methods using skin color information defined based on the prior knowldege, threshold value to be used at the stage of dividing the backround and the skin region was decided on a subjective point of view through experiments. Also, threshold value was selected in a passive manner according to their background and illumination environments in these existing methods. These existing methods displayed a drawback in that their performance was fully influenced by the threshold value estimated through repetitive experiments. To overcome the drawback of existing methods, this paper propose a skin region detection method using a histogram approximation based on the mean shift algorithm. The proposed method is to divide the background region and the skin region by using the mean shift method at the histogram of the skin-map of the input image generated by the comparison of the similarity with the standard skin color at the CbCr color space and actively finding the maximum value converged by brightness level. Since the histogram has a form of discontinuous function accumulated according to the brightness value of the pixel, it gets approximated as a Gaussian Mixture Model (GMM) using the Bezier Curve method. Thus, the proposed method detects the skin region by using the mean shift method and actively finding the maximum value which eventually becomes the dividing point, not by using the manually selected threshold value unlike other existing methods. This method detects the skin region high performance effectively through experiments.

Atomic Absorption Spectrophotometric Analysis of Heavy Metals in Unpolished Rice Grains (원자흡광법(原子吸光法)에 의(依)한 현미중(玄米中)의 중금속(重金屬) 함량분석(含量分析)에 관(關)한 연구(硏究))

  • Park, Seung Heui
    • Korean Journal of Agricultural Science
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    • v.8 no.1
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    • pp.126-132
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    • 1981
  • Rice grains harvested from paddy fields around industrial areas of Kyongin, Iri, and Jeonju were taken and analysed the contents of heavy metals which were considered to be accumulated in those rice grains. Atomic absorption spectrophotometer was applied for analysis of heavy metals such as copper(Cu), zinc(Zn), lead(Pb) and cadmium (Cd) in unpolished rice grains. The content of Cu in rice grains harvested from Iri was the lowest, 2.94 ppm, while those from Seo-Myon, Shiheung-Gun was the highest, 3.65 ppm. The contents of Zn and Pb in rice grains were ranged 20.56~27.21 ppm and 0.38~0.53 ppm, respectively. Cd content were exceptionally lower than the regulated level, 1.0 ppm. The content of this metal detected in rice grains from Seo-Myon was the highest, 0.509 ppm, but those of other areas were much lower, ranged 0.040~0.136 ppm. The present concentrations of Cu, Zn, and Pb in rice grains were also lower than the expected level and below the maximum permissible concentrations.

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Improvement and Validation of Convective Rainfall Rate Retrieved from Visible and Infrared Image Bands of the COMS Satellite (COMS 위성의 가시 및 적외 영상 채널로부터 복원된 대류운의 강우강도 향상과 검증)

  • Moon, Yun Seob;Lee, Kangyeol
    • Journal of the Korean earth science society
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    • v.37 no.7
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    • pp.420-433
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    • 2016
  • The purpose of this study is to improve the calibration matrixes of 2-D and 3-D convective rainfall rates (CRR) using the brightness temperature of the infrared $10.8{\mu}m$ channel (IR), the difference of brightness temperatures between infrared $10.8{\mu}m$ and vapor $6.7{\mu}m$ channels (IR-WV), and the normalized reflectance of the visible channel (VIS) from the COMS satellite and rainfall rate from the weather radar for the period of 75 rainy days from April 22, 2011 to October 22, 2011 in Korea. Especially, the rainfall rate data of the weather radar are used to validate the new 2-D and 3-DCRR calibration matrixes suitable for the Korean peninsula for the period of 24 rainy days in 2011. The 2D and 3D calibration matrixes provide the basic and maximum CRR values ($mm\;h^{-1}$) by multiplying the rain probability matrix, which is calculated by using the number of rainy and no-rainy pixels with associated 2-D (IR, IR-WV) and 3-D (IR, IR-WV, VIS) matrixes, by the mean and maximum rainfall rate matrixes, respectively, which is calculated by dividing the accumulated rainfall rate by the number of rainy pixels and by the product of the maximum rain rate for the calibration period by the number of rain occurrences. Finally, new 2-D and 3-D CRR calibration matrixes are obtained experimentally from the regression analysis of both basic and maximum rainfall rate matrixes. As a result, an area of rainfall rate more than 10 mm/h is magnified in the new ones as well as CRR is shown in lower class ranges in matrixes between IR brightness temperature and IR-WV brightness temperature difference than the existing ones. Accuracy and categorical statistics are computed for the data of CRR events occurred during the given period. The mean error (ME), mean absolute error (MAE), and root mean squire error (RMSE) in new 2-D and 3-D CRR calibrations led to smaller than in the existing ones, where false alarm ratio had decreased, probability of detection had increased a bit, and critical success index scores had improved. To take into account the strong rainfall rate in the weather events such as thunderstorms and typhoon, a moisture correction factor is corrected. This factor is defined as the product of the total precipitable waterby the relative humidity (PW RH), a mean value between surface and 500 hPa level, obtained from a numerical model or the COMS retrieval data. In this study, when the IR cloud top brightness temperature is lower than 210 K and the relative humidity is greater than 40%, the moisture correction factor is empirically scaled from 1.0 to 2.0 basing on PW RH values. Consequently, in applying to this factor in new 2D and 2D CRR calibrations, the ME, MAE, and RMSE are smaller than the new ones.

A STUDY ON POTASSIUM DEFICIENCY SYMPTOMS OF FLUE-CURED TOBACCO. (INTERRELATIONSHIP OF NITROGEN AND POTASSIUM CONTENT IN DIFFERENT POSITION OF VEGETATIVE ORGAN APPLIED WITH DIFFERENT LEVELS OF NITROGEN AND POTASSIUM FERTILIZER) (황색종 잎담배의 칼륨결핍증에 관한 연구 (질소 및 칼륨시비수준에 따른 연초기관부위별 질소와 칼륨함량의 상호관계))

  • Hong, Soon-Dal;Lee, Yun-Hwan;Kim, Jai-Joung
    • Journal of the Korean Society of Tobacco Science
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    • v.6 no.2
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    • pp.199-296
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    • 1984
  • Symptoms of potassium deficiency were studied with flue-cured tobacco (N.C 2326) which had been applied with several levels of nitrogen and potassium fertilizer at 7, 10, and 13 Kg N/10a, and 15, 20, and $25KgK_2O/10a$, respectively. The distributions of T-N, $NH_4-N, \;NO_3-N$, and K contents in 18 leaves from the bottom to the top stalk position were investigated at 63 days after transplanting. The T-N content in the different stalk positions was increased from the bottom to the top ; however, vice versa in K content. As increasing the application levels, the increasing rate of the T-N content in the different leaf fractions were similar to those of rib and laminae. On the contrary, the rates of K content in the rib were higher then that of laminae and in the bottom than the top stalk position. Those indicated that the unbalance of nitrogen and potassium contents in the leaves came to maximum in the laminae of the top stalk position and was possibly affected by the application level of nitrogen rather than potassium. The T-N content in the laminae was higher than that of the rib. However the K and $NO_3$-N contents in the laminae were significantly lower than those of the rib. Therefore the $K^+$ uptake by tobacco was thought to be accompanied with $NO_3$- and transported smoothly up to the rib, but not from the rib to the laminae where assimilation materials were accumulated. Distribution of inorganic components in the different positions of vegetative organ were also investigated at 60 days after transplanting. The T-N and $P_2O_5$ contents were in order as; top > middle> bottom of the stalk and the stalk position, and laminae > vein > midrib of leaf fraction, however vice versa in K content of the stalk position and leaf fraction. In addition distributions of CaO and MgO contents in the each leaf fraction were resemble to that of the T-N content. On the contrary, the distributions of the CaO and MgO contents in the different stalk positions were similar to that of the K content.

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Temperature and Flow Velocity Analysis for Fire in Synthetic Heat Transfer Fluid Boiler (열매유 보일러 내부화재에 따른 온도 및 속도분포 해석)

  • Kim, Yeob-Rae;Son, Bong-Sei
    • Fire Science and Engineering
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    • v.27 no.5
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    • pp.19-25
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    • 2013
  • The fire took place in the synthetic heat transfer fluid boiler. This study uses simulation to investigate the first, second and third passes and the temperature in the fire burner. The boiler's internal fluid is more or less unsteady due to the out of order inverter. As the operation continues, the flame's flow and speed are unsteady. The synthetic heat transfer fluid leak spouted about 120 kg/min in the form of vapor in the early period of the fire. The flame extended to the second and third passes. The highest temperature of the second and third pass is $1059^{\circ}C$ and $1007^{\circ}C$, respectively. The simulation shows that the temperature is $767^{\circ}C$ in the low part of the third pass. The synthetic heat transfer fluid spouted through the cracked part of the fire box in the first pass and accumulated on the turn table. The temperature rises to $183^{\circ}C$ in the low part of the burner. Therefore, it is expected that the temperature of the interior of the fire box is above $1200^{\circ}C$. The temperature of the burner rises to a maximum level several times in a short period. On account of that, several explosions occur in the fire burner.

Factors Regulating the Nitrogen Fixation Activity and Growth of Anabaena variabilis ATCC 29413 (Anabaena variabilis ATCC 29413 의 생장과 질소고정활성의 조절요인)

  • 송승달;한동훈
    • Korean Journal of Microbiology
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    • v.30 no.5
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    • pp.391-396
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    • 1992
  • Anabaena variabilis A TCC 29413. a photoautotrophic and nitrogen fixing cyanobacteria. was investigated on the environmental factors regulating the growth and nitrogen lixation activity. A good growth of cyanobacteria] cells was observed due to nitrogen t1xation by the heterocyst differentiation in nitrogen free Allen and Arnon (]/8) medium. The nitrogenase activity was appeared to be in proportion to the cell growth lor 6 days then drastically decreased in the later growth period when the nitraTe was accumulated to high level in the culture to cause the inhibition. The optima] conditions lilr the cell growth and nitrogenase activity of A. varillbili.l were anaerobic. IO.OO0 lux. $30^{\circ}C$ and pH 8 with the nitrogen Cree minimal medium. The activity was significantly inhihited by the low concentrations of ammonium and nitrate. but was stimulated b) the ]ow Ieve] of phosphate and carbonate sources. The treatments of several toxic heavy metals showed strong inhibition of the cell growth and nitrogenase activity by O.3~10 ppm in the order of $Hg^{2+}$ > $Cd^{2+}$ > $Co^{2+}$ > $Zn^{2+}$ > $Ph^{2+}$, and the concentrations for 50% inhibition of the maximum activity were 0.41. 0.47. 0.5 L 0.66 and 8.1 ppm. respectively. The addition of carbohydrates (0.5~ 1.0%) in the dark condition stimulated the growth and activity in the order of sucrose > fructose > glucose.

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Uranium Levels in Soil and Plant, and Estimation of Its Intake by the Residents at the Uranium Deposited Area (우라늄광 부근에서 우라늄의 토양 및 식물체중 함량과 주민들에 의한 체내 집적량추정)

  • U, Zang-Kual;Song, Ki-Joon;Kim, Tai-Soon
    • Applied Biological Chemistry
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    • v.22 no.4
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    • pp.221-226
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    • 1979
  • The soils and plants were sampled from 26 sites of Deogpyeongri, Goisangun, which had been found to be one of the uranium deposit areas. Uranium levels of the samples were determined and the amount of uranium intake by the residents through the food-chains was estimated. The average uranium concentration of Deogpyeongri soils was 15.5ppm with a range of from 4.9 to 43.6ppm showing rather higher values than those of control area, Yangjugun and Icheongun, Gyeonggi-do. The average uranium content of the plant samples from Deogpyeongri was 0.69ppm, about twice the uranium concentration of the control samples. The daily intake of uranium by an adult lived on the agricultural food stuffs produced in Deogpyeongri, was estimated to be about $247{\mu}g$, eqivalent to $0.83{\times}10^{-4}{\mu}Ci$, which is much higher activity compared to the daily intake of uranium by New York citizen, $1.3{\mu}g$. However the calculated uranium level accumulated in the human body of Deogpyeong area was $2.03{\times}10^{-4}{\mu}Ci$ which is still lower than $0.2{\mu}Ci$, the maximum permissible burden in total body recommended by the ICRP.

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