• 한국수산과학회지
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• 제7권4호
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• pp.209-214
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• 1974

Casein을 단백원으로 하고 조단백질과 지질의 함량을 조사한 사료로서 치무지개송어를 19일간 사육하여 다음과 같은 결과를 얻었다. 1) 무지개송어의 체중은 사료중의 단백질 함량이 많을수록 증가하며 단백질의 함량 $40\%$ 부근에서 최대치에 달하고 그 이상 단백질이 증가하여도 체중은 증가하지 않았다. 체단백질의 증가도 사료중의 단백질 함량이 $40\%$ 부근에서 최대치에 가까워지며 그 이상 단백질이 증가하여 도 변화는 적었다. 2) 단백질의 이용치는 사료중의 단백질 함양이 많을 수록 증가하였으나 체단백질의 축적률은 단백질의 함량 $40\%$ 부근에서 최대치에 달했다. 3) 이상의 결과에서 Casein을 단백원으로 하는 경우 치무지개송어의 단백질 요구량은 사료중 $40\%$라고 생각했다. 4) 사료의 단백효율(y)은 단백질의 함양(x)이 많을 수록 감소하고 그 관계는 y=4.91-0.034x의 방정식으로 표시 할 수 있었다. 5) 사료의 단백질 함량과 사료효율 및 사료계수와의 관계를 검토하였다. 끝으로 시종 실험을 지도하여 주신 동경수산대학에 야진길 교수에게 사의를 표한다.

• Journal of Plant Biotechnology
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• 제1권2호
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• pp.117-121
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• 1999

Effects of jasmonic acid treatment, UV-B and white light treatment on the anthocyanin biosynthesis and cell growth were investigated using the cell suspension culture system of grape (Vitis vinifera L.). Cell growth was not affected by white light irradiation, while it was remarkably suppressed by UV-B irradiation from 8 to 32 h. Anthocyanin accumulation dramatically increased after 16 h from irradiation of UV-B. Simultaneous treatment of jasmonic acid and UV-B increased anthocyanin accumulation by 10-fold. The cell division was restored when anthocyanin was abundantly accumulated after 32 h from UV-B irradiation. Optimum concentration of jasmonic acid was found to be 5 uM for maximum accumulation of anthocyanin. Application of jasmonic acid to grape suspension cells rapidly induced the expression of CHS gene after 2 h from treatment and showed maximum level at 32 h. Simultaneous treatment of jasmonic acid and light also induced CHS gene expression after 2 h, but the maximum level of CHS transcript was observed at 16 h with white light and 8 h with UV-B exposure. The synergistical effects could be explained by the defense mechanism that UV irradiation is mediated in part by alterations in JA and its signaling pathway.

• 유기물자원화
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• 제5권2호
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• pp.39-45
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• 1997

카드뮴(Cd)에 대한 지렁이의 독성한계를 결정하고, 생물적 농축능력을 평가함으로써, 잠재적 독성 원소가 높은 유기성 폐기물의 안전한 처리를 위한 vermicomposting의 이용가능성을 검토하였다. 우분에 첨가한 카드뮴 함량은 0 (대조구), 50, 100, 200, 400, 800, 1600 mg/kg의 7수준 이였다. 우분내 카드뮴 함량의 차이에 따른 지렁이의 생존율, 사육종료시의 개체당 평균생체중, 증체율, 난포수, 산자수 및 잔식량에서는 유의한 차이가 인정되지 않아서, 우분내 카드뮴 함량이 1600mg/kg까지는 지렁이의 생육에 별다른 영향을 미치지 않는 것으로 판단되었다. 생육형질간의 상호관계를 살펴본 결과, 생존율과 난포수간에는 0.1% 수준의 유의성이 인정되었고, 실험종료시 평균생체중과 잔식량간에는 5% 수준의 유의한 부(-)의 상관이 인정되었다. 지렁이 체내의 카드뮴 축적량은 카드뮴 함량 956.8~1116.6 mg/kg범위에서 최대 축적량인 283.8~396.2 mg/kg을 나타낸 후 점차 감소하였다.

• Journal of Microbiology and Biotechnology
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• 제13권1호
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• pp.85-89
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• 2003

The difference in the thermotolerance between Saccharomyces cerevisiae KNU5377 and ATCC24858 was compared by assaying the amounts of trehalose accumulated under growth and heat shock conditions. Both strains exhibited similar trehalose accumulation during the growth period, but an intrinsic thermotolerance was much higher in KNU5377 than in the control strain. This result implied that some strain-specific characteristics of KNU5377, other than trehalose accumulation, primarily were responsible fur its higher intrinsic thermotolerance. Heat shock at $43^{\circ}C$ for 90 min to the exponentially growing cells resulted in the maximum level of trehalose In both strains. Trehalose accumulated at least twice more in KNU5377 by the heat shock than in the control, due to the maintenance of its neutral trehalase activity even after the heat shock. Consequently, the Increase of acquired thermotolerance in both strains correlated with an increase in the trehalose content in each strain. In conclusion, KNU5377 exhibited a well-modulated trehalose-related mechanism to accumulate more trehalose by means of maintaining neutral trehalase activity after heat shock than the control strain, thereby contributing to its acquired thermotolerance.

• 지질공학
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• 제19권1호
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• pp.25-31
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• 2009

본 연구는 수치해석을 이용하여 강우강도와 사면 안정성의 상관성을 파악하기 위하여 수행되었다. 지난 36년간의 기상청 자료를 조사하여 10분, 1시간, 일 최다강수량이 각각 28 mm, 70 mm, 271 mm로 집계되었다. 그때 침투류 해석은 프로그램 SLEEP/W을 이용하여 사면의 지하수위를 획득하기 위해 수행되었고, 안정성 분석은 프로그램 SLOPE/W을 이용하여 시간 단계별로 수행되었다. 10분 강수량 28 mm일 경우 2시간, 1시간 강수량 70 mm일 경우 7시간, 1일 강수량 271 mm일 경우 72시간에 사면내의 지하수위가 포화상태에 도달하였다. 10분 강우강도가 28 mm일 경우, 누적강우량 196 mm 일 때, 안전율이 1.0 이하가 되었으며, 10분 강우강도가 13 mm, 1.9 mm 일 경우에는 누적강우량이 각각 468 mm, 820 mm 일 때, 안전율이 1.0 이하가 되었다. 이 결과는 누적강우량보다는 강우강도가 사면안정성에 더 큰 영향을 미치고 있다는 것을 시사한다.

• Journal of Microbiology and Biotechnology
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• 제19권10호
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• pp.1142-1149
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• 2009

Several bioactive metabolites, including pyrrolnitrin, N-acylhomoserine lactones, and polyhydroxyalkanoates were isolated from Burkholderia sp. O33. Effects of various nutrients, including sugars, gluconolactone, glycerol, tryptophan, chloride, and zinc were investigated in relation to the production of these metabolites. Logarithmic increase of pyrrolnitrin was observed between 2-5 days and reached a maximum at 7-10 days. Tryptophan concentration reached the maximum at 3 days, whereas 7-chlorotryptophan was gradually increased throughout the studies. Among various carbon sources, gluconolactone, trehalose, and glycerol enhanced pyrrolnitrin production, whereas strong inhibitory effects were found with glucose. Relative concentrations of pyrrolnitrin and its precursors were in the order of pyrrolnitrin$\gg$dechloroaminopyrrolnitrin or aminopyrrolnitrin throughout the experiments. Among three N-acylhomoserine lactones, the N-octanoyl analog was the most abundant quorum sensing signal, of which the concentrations reached the maximum in 2-3 days, followed by a rapid dissipation to trace level. No significant changes in pyrrolnitrin biosynthesis were observed by external addition of N-acylhomoserine lactones. Polyhydroxyalkanoates accumulated up to 3-4 days and decreased slowly thereafter. According to the kinetic analyses, no strong correlations were found between the levels of pyrrolnitrin, N-acylhomoserine lactones, and polyhydroxyalkanoates.

• Journal of Plant Biotechnology
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• 제32권1호
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• pp.57-61
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• 2005

생물반응기를 이용한 섬오갈피 부정근 배양시 첨가되는 생장조절제의 종류와 농도에 따른 부정근의 생장과 이차대사산물 생산과의 관계를 조사하였다. IBA 농도별 부정근 생장은 IBA $2.0\;\cal{mg/L}$에서 6.7 g DW/L로 가장 높았으나, eleutheroside E 함량은 IBA 농도에 따라 차이가 없었다 TDZ 농도에 따른 부정근 생장은 농도가 증가함에 따라 생장도 증가하여 eleutheroside E 함량은 IBA $3.0\;\cal{mg/L}$에 TDZ $0.1\;\cal{mg/L}$ 처리구에서 $3,327\;{\mu}g/g$ DW로 가장 생산량이 많았다. 이와 달리 eleutheroside B ($153\;{\mu}g/g$ DW)과 chlorogenic acid ($7.7\;\cal{mg/g}$ DW)는 IBA $5.0\;\cal{mg/L}$에 TDZ $0.1\;\cal{mg/L}$ 처리구에서 함량이 가장 높았다. Zeatin은 부정근 생육과 eleutheroside E 함량에는 크게 영향을 미치지 않으나 eleuthe-roside B와 chlorogenic acid 생산을 크게 억제하였다.

• Transactions on Electrical and Electronic Materials
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• 제13권1호
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• pp.10-15
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• 2012

In conventional, skin detection methods using for skin color definitions is based on prior knowledge. By experimentation, the threshold value for dividing the background from the skin region is determined subjectively. A drawback of such techniques is that their performance is dependent on a threshold value which is estimated from repeated experiments. To overcome this, the present paper introduces a skin region detection method. This method uses a histogram approximation based on the mean shift algorithm. This proposed method applies the mean shift procedure to a histogram of a skin map of the input image. It is generated by comparing with the standard skin colors in the $C_bC_r$ color space. It divides the background from the skin region by selecting the maximum value according to the brightness level. As the histogram has the form of a discontinuous function. It is accumulated according to the brightness values of the pixels. It is then, approximated by a Gaussian mixture model (GMM) using the Bezier curve technique. Thus, the proposed method detects the skin region using the mean shift procedure to determine a maximum value. Rather than using a manually selected threshold value, as in existing techniques this becomes the dividing point. Experiments confirm that the new procedure effectively detects the skin region.

• 미생물학회지
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• 제26권2호
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• pp.137-142
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• 1988

pH decreased as the substrate mycelium developed, $\Delta$pH was 1.05-1.15, but increased after the aerial mycelium formation. The lactic acid content in culture solution showed no difference between 0.2% and 5% glucose, at which the aerial mycelium formation was repressed. The growth and development of mycelium was delayed by the lactate treatment. The activity of catalase was maximum in 24 hours after inoculation, and the wuperoxide dismutase activity showed a constant level during the developmental phases. The ascorbic acid accumulated after the aerial mycelium formation. The ascorbate oxidase isozyme of Rf 0.44 appeared, while the isozyme of Rf 0.36 desappeared during the development.

• Journal of Biosystems Engineering
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• 제32권3호
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• pp.145-152
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• 2007

Uniformity of spray deposit is one of the important factors in spray performance affecting efficacy of pest management. Distributions of spray deposit on artificial targets were measured and analyzed to enhance the efficiency of spray application. The research was studied to understand the deposition characteristics of spray droplets and to determine the optimum conditions of chemical application. The deposit and its pattern by the lower fan speed was more uniform and higher than that by the higher fan speed. The upward blasting distance was limited within 3 m, but the limit to the ground level was expanded the distance more than 3.5 m because of the accumulated droplets. When the fan speed was higher at the distance of 2.5 m, deposit reached to maximum. When the distance increased, deposit was getting lower. At the both fan speeds, the deposit was concentrated below $30^{\circ}$ because of the gravitation and the resistance of wind. This research can be useful in designing an orchard sprayer and its operation for various tree canopies. To achieve a uniform distribution of deposit using the air-blast type orchard sprayer, the application rate from the middle boom should be increased as the air velocity to the upward increased. The spray rate to the side boom should be limited in a minimal level.