• Korean Journal of Fisheries and Aquatic Sciences
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• v.7 no.4
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• pp.209-214
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• 1974

The utilization dietary protein in young rainbow trout was investigated when feeded with the diets of controlled levels of casein and lipids for 12 days. The composition of the diet is shown in Table 1. Body weight gain was in proportion to the protein content in diet up to $40\%$, ana the maximum was obtained with $40.4\%$ of crude protein (Fig. 1, Table 2). On the other hand, the accumulated protein in body also showed almost maximum value around 40n of protein level in the diet and: the change of accumulated protein showed a little with more-protein level(Fig. 3, Table 9). The protein accumulation rate (protein accumulated/protein consumed) showed its highest value at about $40\%$ of protein level, while the protein utilization value (protein accumulation rate x protein content of diet) attained its highest value at $54.8\%$ of protein level (Table 3). With the above results it is observed that the requirement of dietary protein for young rainbow trout is about $40\%$ of protein in the diet when casein is used as the sole protein source. In protein efficiency ratio (PER) the lower protein level in the diet, the higher PER yields and the more the quantity of protein increases, the more PER decreases and its relation could be figured out as an equation of y=4.91-0.034x (Fig.4), Nose measured PER utilizing the diet which result of this, it is reported that PER rate of casein dropped within the extent of $25\%$ protein in diet. The reason why such a different PER rate appeared at the low protein level is revealed as the carbohydrate is low but the lipid high in capacity of utililzing nutrients for rainbow trout. The relation between the protein content of diet and the conversion factor, feed efficiency were determined and the results are shown in Fig. 2.

• Journal of Plant Biotechnology
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• v.1 no.2
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• pp.117-121
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• 1999

Effects of jasmonic acid treatment, UV-B and white light treatment on the anthocyanin biosynthesis and cell growth were investigated using the cell suspension culture system of grape (Vitis vinifera L.). Cell growth was not affected by white light irradiation, while it was remarkably suppressed by UV-B irradiation from 8 to 32 h. Anthocyanin accumulation dramatically increased after 16 h from irradiation of UV-B. Simultaneous treatment of jasmonic acid and UV-B increased anthocyanin accumulation by 10-fold. The cell division was restored when anthocyanin was abundantly accumulated after 32 h from UV-B irradiation. Optimum concentration of jasmonic acid was found to be 5 uM for maximum accumulation of anthocyanin. Application of jasmonic acid to grape suspension cells rapidly induced the expression of CHS gene after 2 h from treatment and showed maximum level at 32 h. Simultaneous treatment of jasmonic acid and light also induced CHS gene expression after 2 h, but the maximum level of CHS transcript was observed at 16 h with white light and 8 h with UV-B exposure. The synergistical effects could be explained by the defense mechanism that UV irradiation is mediated in part by alterations in JA and its signaling pathway.

• Journal of the Korea Organic Resources Recycling Association
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• v.5 no.2
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• pp.39-45
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• 1997

This study was performed to investigate the growth response of earthworm (Eisenia foetida) in cow manure added Cadmium (Cd) and therefore, to estimate the toxic threshold level of Cadmium and the levels of Cadmium in cow manure for obtain-ing the maximum Cadmium-concentration enable to accumulate in earthworm tis-sue. Seven different concentrations of Cadmium added in cow manure were 0 (control), 50, 100, 200, 400, 800, 1600 mg/kg, respectively. Survival rate (SR), mean fresh weight of adult worm at final time ($FW_2$), increasing rate (IR), number of cocoon (NC), number of young worm (NY) and residual matter (RW) were not significantly different among Cadmium concentrations in cow manure. Of the measured growth-characteristics, survival rate (SR) had a significantly positive correlation (p<0.001) with number of cocoon (NC). But mean fresh weight of adult worm at final time ($FW_2$) had a significantly negative correlation (p<0.05) with residual matter of cow manure. The maximum Cadmium concentration accumulated in tissue was ranged from 283.8 mg/kg to 396.2 mg/kg. It was obtained at the level of 956.5 mg/kg and 1116.6 mg/kg in cow manure added Cadmium, respectively.

• Journal of Microbiology and Biotechnology
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• v.13 no.1
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• pp.85-89
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• 2003

The difference in the thermotolerance between Saccharomyces cerevisiae KNU5377 and ATCC24858 was compared by assaying the amounts of trehalose accumulated under growth and heat shock conditions. Both strains exhibited similar trehalose accumulation during the growth period, but an intrinsic thermotolerance was much higher in KNU5377 than in the control strain. This result implied that some strain-specific characteristics of KNU5377, other than trehalose accumulation, primarily were responsible fur its higher intrinsic thermotolerance. Heat shock at $43^{\circ}C$ for 90 min to the exponentially growing cells resulted in the maximum level of trehalose In both strains. Trehalose accumulated at least twice more in KNU5377 by the heat shock than in the control, due to the maintenance of its neutral trehalase activity even after the heat shock. Consequently, the Increase of acquired thermotolerance in both strains correlated with an increase in the trehalose content in each strain. In conclusion, KNU5377 exhibited a well-modulated trehalose-related mechanism to accumulate more trehalose by means of maintaining neutral trehalase activity after heat shock than the control strain, thereby contributing to its acquired thermotolerance.

• The Journal of Engineering Geology
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• v.19 no.1
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• pp.25-31
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• 2009

This study was conducted to characterize the relationship of rainfall intensity and slope stability by using numerical analysis. The maximum precipitation rate for 10 minutes, 1 hour and 1 day was determined as 28 mm, 70 mm and 271 mm, respectively, by investigating 36 years of KMA data. Then slope infiltration analysis was performed to obtain the ground water level in the slope by using computer programming SEEP/W, and slope stability analysis was done for each time step by using program SLOPE/W. The factor of safety was minimized when the slope was saturated under each rainfall intensity; the time required for saturation was 2 hours with 10 minutes rainfall intensity of 28 mm, 7 hours with 1 hour rainfall intensity of 70 mm and 3 days with 1 day rainfall intensity of 271 mm. When accumulated rainfall was 196 mm for the 10minutes rainfall intensity of 28 mm with duration of 2 hours, the factor of safety was decreased to 1.0, while accumulated rainfall of 468 mm and 820 mm for the 1 hour and 1 day rainfall intensity, respectively, was required to reach the factor of safety, 1.0. Since the normalized rainfall intensity was 13 mm and 1.9 mm for 1 hour and 1 day maximum rainfall, respectively, those results showed that the rainfall intensity could have a more effect on the slope stability than the accumulated rainfall.

• Journal of Microbiology and Biotechnology
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• v.19 no.10
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• pp.1142-1149
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• 2009

Several bioactive metabolites, including pyrrolnitrin, N-acylhomoserine lactones, and polyhydroxyalkanoates were isolated from Burkholderia sp. O33. Effects of various nutrients, including sugars, gluconolactone, glycerol, tryptophan, chloride, and zinc were investigated in relation to the production of these metabolites. Logarithmic increase of pyrrolnitrin was observed between 2-5 days and reached a maximum at 7-10 days. Tryptophan concentration reached the maximum at 3 days, whereas 7-chlorotryptophan was gradually increased throughout the studies. Among various carbon sources, gluconolactone, trehalose, and glycerol enhanced pyrrolnitrin production, whereas strong inhibitory effects were found with glucose. Relative concentrations of pyrrolnitrin and its precursors were in the order of pyrrolnitrin$\gg$dechloroaminopyrrolnitrin or aminopyrrolnitrin throughout the experiments. Among three N-acylhomoserine lactones, the N-octanoyl analog was the most abundant quorum sensing signal, of which the concentrations reached the maximum in 2-3 days, followed by a rapid dissipation to trace level. No significant changes in pyrrolnitrin biosynthesis were observed by external addition of N-acylhomoserine lactones. Polyhydroxyalkanoates accumulated up to 3-4 days and decreased slowly thereafter. According to the kinetic analyses, no strong correlations were found between the levels of pyrrolnitrin, N-acylhomoserine lactones, and polyhydroxyalkanoates.

• Journal of Plant Biotechnology
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• v.32 no.1
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• pp.57-61
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• 2005

The influence of different growth regulators on biomass of adventitious roots and secondary metabolites accumulation in bioreactor cultures of Eleutherococcus koreanum was studied. The maximum growth of adventitious roots was observed in the presence of $2.0\;\cal{mg/L}$ IBA (6.7 g DW/L). However the productivity of eleutheroside E was not significantly different among IBA levels. High level of thidiazuron (TDZ) efficiently increased both root growth and secondary metabolites production. Especially when $0.1\;\cal{mg/L}$ TDZ was combined with $3.0\;\cal{mg/L}$ IBA, the highest eleutheroside E accumulation ($3,327{\mu}g/g $DW) was observed. When the same TDZ level was treated in combination with $5.0\;\cal{mg/L}$ IBA, both chlorogrnic acid and eleutheroside B were accumulated to maximum level. In contrast, zeatin remarkably suppressed the accumulation of both eleutheroside B and chlorogenic acid.

• Transactions on Electrical and Electronic Materials
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• v.13 no.1
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• pp.10-15
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• 2012

In conventional, skin detection methods using for skin color definitions is based on prior knowledge. By experimentation, the threshold value for dividing the background from the skin region is determined subjectively. A drawback of such techniques is that their performance is dependent on a threshold value which is estimated from repeated experiments. To overcome this, the present paper introduces a skin region detection method. This method uses a histogram approximation based on the mean shift algorithm. This proposed method applies the mean shift procedure to a histogram of a skin map of the input image. It is generated by comparing with the standard skin colors in the $C_bC_r$ color space. It divides the background from the skin region by selecting the maximum value according to the brightness level. As the histogram has the form of a discontinuous function. It is accumulated according to the brightness values of the pixels. It is then, approximated by a Gaussian mixture model (GMM) using the Bezier curve technique. Thus, the proposed method detects the skin region using the mean shift procedure to determine a maximum value. Rather than using a manually selected threshold value, as in existing techniques this becomes the dividing point. Experiments confirm that the new procedure effectively detects the skin region.

• Korean Journal of Microbiology
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• v.26 no.2
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• pp.137-142
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• 1988

pH decreased as the substrate mycelium developed, $\Delta$pH was 1.05-1.15, but increased after the aerial mycelium formation. The lactic acid content in culture solution showed no difference between 0.2% and 5% glucose, at which the aerial mycelium formation was repressed. The growth and development of mycelium was delayed by the lactate treatment. The activity of catalase was maximum in 24 hours after inoculation, and the wuperoxide dismutase activity showed a constant level during the developmental phases. The ascorbic acid accumulated after the aerial mycelium formation. The ascorbate oxidase isozyme of Rf 0.44 appeared, while the isozyme of Rf 0.36 desappeared during the development.

• Journal of Biosystems Engineering
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• v.32 no.3
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• pp.145-152
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• 2007

Uniformity of spray deposit is one of the important factors in spray performance affecting efficacy of pest management. Distributions of spray deposit on artificial targets were measured and analyzed to enhance the efficiency of spray application. The research was studied to understand the deposition characteristics of spray droplets and to determine the optimum conditions of chemical application. The deposit and its pattern by the lower fan speed was more uniform and higher than that by the higher fan speed. The upward blasting distance was limited within 3 m, but the limit to the ground level was expanded the distance more than 3.5 m because of the accumulated droplets. When the fan speed was higher at the distance of 2.5 m, deposit reached to maximum. When the distance increased, deposit was getting lower. At the both fan speeds, the deposit was concentrated below $30^{\circ}$ because of the gravitation and the resistance of wind. This research can be useful in designing an orchard sprayer and its operation for various tree canopies. To achieve a uniform distribution of deposit using the air-blast type orchard sprayer, the application rate from the middle boom should be increased as the air velocity to the upward increased. The spray rate to the side boom should be limited in a minimal level.