• 제목/요약/키워드: accelerated experiment

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Effect of Waste Sludge of Fermentation By-Product on the Growth of Young Radish and Chemical Properties of Soil (발효부산물 오니의 시용이 열무 생장과 토양화학성에 미치는 영향)

  • Hong, Soon-Dal;Seok, Yeong-Seon;Sa, Tong-Min
    • Korean Journal of Environmental Agriculture
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    • v.20 no.1
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    • pp.8-14
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    • 2001
  • To investigate the effects of waste sludge from antibiotic fermentation on the growth of young radish and chemical properties of soil, five levels of fertilizer, control (recommended fertilizer, $N-P_2O_5-K_2O=160-59-104$ kg/ha), AS(control + sludge 1,000 kg/ha), AC(control + conventional compost 1,000 kg/ha), SNS(control - subtracting 30% N of sludge + sludge 1,000 kg/ha) and SNC(control - subtracting 30% N of conventional compost + conventional compost 1,000 kg/ha) were applied and radish was grown twice with same treatments on May and August in 1998. Germination rate and early growth of young radish grown with AS and SNS were lower than those grown in control and with AC, SNC. This negative effects by adding the sludge in the early growth seemed to be caused by damage of ammonia gas released during degradation of the sludge in soil. However, yield of young radish showed no significant difference among all the treatments including the AS and SNS at the 1st and 2nd experiment, and these suggested that the latter half of growth of young radish was accelerated by adding the sludge. Contents of T-N in young radish and inorganic N in soil showed a tendency to increase by adding the sludge while antibiotic substance, cephalosporin-C, was not detected in plant material and soils after harvest of young radish in both experiments. Consequently, waste sludge from antibiotic fermentation, which contains high levels of organic matter and nitrogen could be used as an useful resource in agriculture.

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Relationship between Seed Size and Seed Vigor in Soybean. (콩의 종실크기와 종자활성 간의 관계)

  • 박금룡;최원열;정동희;김석동
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.2
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    • pp.139-150
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    • 1993
  • This experiment was carried out to investigate the difference of seed vigor according to seed size, and the physiological aspects and physico-chemical phenomena related with seed vigor in soybean cultivars. Portion of seed coat and radicle to entire seed was much higher in cultivars with small seed than with large. Seed coat rate ranged 7.9% to 9.9%, and radicle 2.5% to 3.3% in small seed group, Whereas in large seed, seed coat rate did 5.5-6.4% and radicle, 1.5 to 2.1%. After accelerated aging treatment, there are significant difference in germination ability between seed size. The germination rate after aging ranged 47 to 80% in cultivars with small seed, but in large seed, only 14 to 24%. After seed was carried out dehydration in incubator at 25 after soaking for 6 hours, the moisture content of seed in drying for 12 hour was 25.5% in small seed, while it was 51% in large seed. Electrical conductivity, leaching soluble nitrogen and sugar content were higher in large seed cultivars. Besides, cotyledon damage after soaking was occurred frequently in large seed cultivars, and seed vigor within same cultivars was higher in small seed than large.

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An Experimental Study on Alkali-Silica Reaction of Alkali-Activated Ground Granulated Blast Furnace Slag Mortars (알칼리 활성 고로슬래그 미분말 모르터의 알칼리-실리카 반응에 관한 실험적 연구)

  • Kim, Young-Soo;Moon, Dong-Il;Lee, Dong-Woon
    • Journal of the Korea Institute of Building Construction
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    • v.11 no.4
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    • pp.345-352
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    • 2011
  • The purpose of this study was to investigate the expansion of alkali-activated mortar based on ground granulated blast furnace slag containing reactive aggregate due to alkali-silica reaction. In addition, this study was particularly concerned with the behavior of these alkaline materials in the presence of reactive aggregates. The experimental program included expansion measurement of the mortar bar specimens, as well as the determination of the morphology and composition of the alkali-silica reaction products by using scanning electron microscopy(SEM), and energy dispersive x-ray(EDX). The experiment showed that while alkali-activated ground granulated blast furnace slag mortars showed expansion due to the alkali-silica reaction, the expansion was 0.1% at Curing Day 14, showing that it is safe. After the accelerated test, SEM and BEM analysis showed the presence of alkali-silica gel and rim around the aggregate and cement paste. According to the EDX, the reaction products decreased markedly as alkali-activated ground granulated blast furnace slag was used. In addition, for the substitutive materials of mineral admixture, a further study on improving the quality of alkali-activated ground granulated blast furnace slag is needed to assure of the durability properties of concrete.

Long-Term Compressive Strength and Durability Properties of "CSG" Materials by Freezing-Thawing Test (동결융해시험에 의한 "CSG" 재료의 장기강도 및 내구 특성)

  • Jin, Guangri;Kim, Kiyoung;Moon, Hongduk;Quan, Hechun
    • Journal of the Korean GEO-environmental Society
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    • v.17 no.12
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    • pp.35-43
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    • 2016
  • With the development of construction technology, constructions of dam and levee (dike) as well as the environmental problems are becoming issues. Recently, many countries have tried to develop and used CSG (Cemented Sand and Gravel), which needs fewer requirements than others in aggregates, constructability and ground condition during the dam construction. Mixing up with small amount of cement, CSG is able to increase the strength and proceed accelerated construction without artificial gradation adjustment of riverbed aggregate and crushed rock on construction site. Thus, CSG can minimize environmental damage resulted from quarries mining and reduce cost of construction. Unlike heat of hydration condition that regular concrete usually met, CSG exposes to repeated dry-wet and freezing and thawing environment. Thus, consider the importance of structure of dam or levee, intensive study on the durability of CSG is needed. In this study, freezing and thawing experiment was carried out to evaluate the durability of CSG. In results, the durability factor of CSG is 30~40 or >40 when the amount of cement is $0.4{\sim}0.6kN/m^3$ or $0.8{\sim}1.0kN/m^3$, respectively. The unconfined compressive strength is reduced to 30~50% or 40~70% when the amount of cement is $0.4{\sim}0.6kN/m^3$ or $0.8{\sim}1.0kN/m^3$, respectively. Taken together, the strength and durability of CSG is reliable when the amount of cement is over $0.8kN/m^3$.

EFFECTS OF AMYLASE ON THE DEMINERALIZATION IN HYDROXYAPATITE (Amylase가 Hydroxyapatite 탈회에 미치는 영향)

  • Lee, In-Hwan;Seo, Jeong-Taeg;Choi, Byung-Jai;Lee, Jong-Gap
    • Journal of the korean academy of Pediatric Dentistry
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    • v.25 no.2
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    • pp.335-351
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    • 1998
  • Salivary proteins which are produced in the saliary acinar cells have been known to be involved in the Calcium and phosphate metabolism. The acquired pellicle resulting from such metabolism is considered as a secondary defence membrane against tooth caries. In this respect, some proteins included in saliva probably play an important role in the prevention of demineralization in enamel. On the other hand, fluoride has long been known to prevent the demineralization of enamel by the inhibition of the growth of Streptococcus mutans(S. mutans) and by the chemical reaction with calcium and phosphate, Therefore, I have examined the roles of amylase and albumin in the demineralization of enamel and compared these preteins with fluoride in terms of anticariogenic effect. 1. The demineralization caused by S. mutans occurred slowly and progressively for the first 60 min, then the rate of demineralization was accelerated afterwards. 2. pH decreased continuously during the entire period of each experiment. 3. The demineralization was significantly inhibited by the preteatment of amylase and fluoride but albumin had little effect on it. 4. An addition of 0.1 mM lactic acid (final concentration 0.1 ${\mu}M$) caused a rapid increase in calcium concentration reaching a maximum within 10 min. 5. pH decreased rapidly by the addition of 0.1 mM lactic acid and reached a minimum within a few seconds followed by an increase in pH. pH reaced a plateu with 10 min. 6. Fluoride, amylase and albumin played little role in the 0.1 mM lactic acid-induced demineralization. 7. A slow infusion of 0.1 M lactic acid at a rate of 5 ${\mu}l/min$ caused a slower increase in calcium concentration compared with the bolus addition of lactic acid. 8. Fluoride had an inhibitory effect on the calcium release caused by slow infusion of lactic acid while amylase and albumin had no effect on it. These results suggest that fluoride inhibits demineralization by protecting the HA from the acid attack whereas amylase has a direct effect on S. mutans to prevent demineralization.

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Effects of the Intervention of Thinking Science Program on Cognitive Development of the 7th Grade Student (Thinking Science 프로그램의 적용이 중학교 1학년 학생들의 인지발달에 미치는 영향)

  • Choi, Byung-Soon;Choi, Mee-Hwa;Nam, Jeong-Hee;Lee, Sang-Kwon
    • Journal of The Korean Association For Science Education
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    • v.22 no.3
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    • pp.422-431
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    • 2002
  • Because of the gap between the cognitive levels of the students and the curricular materials for the students to understand, the secondary school students feel science difficult and they get to avoid science as they go up to the higher grade. So it is absolutely needed to take the active measures to improve the cognitive development of the students through some special programs stimulating their cognitive process. This study investigated the effects of thinking science activity program devised for cognitive acceleration of the students. After implementing thinking science program to 181 7th grade students, the effectiveness of this program was examined through the analysis of covariance of both experimental and control groups. The result of the study showed that the cognitive level of the students in experiment group dealt with thinking science program was more accelerated than that of the students in control group who were just taught regular science curriculum. Especially, the effect was clear to the students in stages 1 and 2B. It was also found that the percentage of the students who promoted from concrete operational stage to formal operational stage was higher in experimental group than in control group. The results of the study implied that cognitive acceleration of the students might be possible through the specially designed materials such as thinking science program.

Search of Experimental Studies(35) on Anti aging and Anti oxidant in Korea (국내문헌(國內文獻)(35)에 나타난 항노화(抗老化) 및 항산화(抗酸化)의 실험적(實驗的) 연구(硏究)에 대(對)한 검색(檢索))

  • Ahn, Sang-Won;Lee, Cheol-Wan
    • The Journal of Korean Medicine
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    • v.19 no.2
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    • pp.373-390
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    • 1998
  • 35 theses conducted in Korea on the topic of senescence or anti-oxidant were classified on the basis of research methods, animals used for the experiments, and research items. Evaluating these research works with respect to the Free Radical Theory, the following conclusions were reached. 1. Of the 17 theses written in the Oriental medicine aspect, three theses used a single herb, nine theses used a complex prescription, four theses concentrated on the usage of the medicinal acupuncture, and one research paper focused on using scientific components. Common objection of these papers were on the verification of the efficacy of herbs. 2. Of the 18 these written in the Western medicine aspect, five theses used a single drug, seven theses conducted a research on the changes due to senescence, and 6 papers were on variety of topics. The main focus of these works were on the mechanism and pathology related to the senescence rather than on the suppression of senescence. 3. Among the theses written in the Oriental medicine perspective, a total of 48 herbs were utilized. 26 of these herbs has a tonification function on the Kidney. Six out of nine complex prescription mentioned above has a function of tonifying the Kidney. 4. With respect to the research subjects used on the experiments, 8 theses have used Senescence-Accelerated mice, 13 theses have used Sprague-Dawley mice, and remaining 7 papers have used human or other animals. 5. These are the categorization of the research items used: the weight (11) and weight changes of the visceral organs (9), the measurement of the content of peroxide-disease (9), the measurement of enzyme vitality (21), the blood and urine test (10), the experiment concerning immune system (3), the influence on the hepatic capability of metabolizing foreign substance (3), the effect on hepatic cell protection (3), the measurement of both the suppression of Free Radical and ability to create Free Radical (2), the measurement of effect of suppresing MDA(malondialdehyde) (4), the effect of eliminating DPPH Radical (2), and experiements about the functions (2). 6. The rate of vitalization of well known anti-oxidants such as Superoxide dismutase (SOD), Protein-bound SH, Nonprotein-bound SH, Glutathione(GSH), Catalase, and etc. were tested in 17 theses. Considering the conclusions mentioned above, the theses related to the senescence published in Korea elected different animals used for experiments, research items and the methods of research, the end result seems to be a lack of objectivity. Thus, I would argue that research methods to overcome such a deficiency need to be developed systematically.

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Effect of Hwanggumgung, a Natural Product, on Hair Growth Promotion in C57BL6 Mice (C57BL6 마우스에서 복합생약제인 황금궁의 육모 효과)

  • Hue Jin-Joo;Li Lan;Lyu Sul-Hye;Baek In-Jeoung;Yon Jung-Min;Nam Sang-Yoon;Yun Young Won;Hwang Seock-Yeon;Hong Jin Tae;Lee Beom Jun
    • YAKHAK HOEJI
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    • v.49 no.6
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    • pp.518-526
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    • 2005
  • Hwanggumgung (HGG) is a hair-care product which is composed of several plant extracts used in oriental medicine. This study was carried out to investigate effect of HGG on hair regrowth in a shaving model of C57BL6 mice. Five-week-old mice were acclimated for 1 week under 23$\pm$3$^{\circ}C$, 50$\pm10\%$ relative humidity and 12 h of a light/dark cycle before beginning experiment. There were four experimental groups including distilled water (D.W., control), 10$\%$ ethanol (EtOH, vehicle control), a positive control of 3$\%$ minoxidil (MXD), and HGG for female and male mice, respectively; Six-weeks old mice were trimmed by electric clippers so as not to damage the skin. The next day; mice without visible scraches were selected, randomized and separated in groups of 11 mice. The test compounds were topically treated with 0.15ml per mouse per day for 21 days. The hair regrowth was photographically and histologically determined during the experimental period of 21 days. Enzyme activities of $\gamma$-glutamyl transpeptidase and alkaline phosphatase were also determined using a rate assay method. There were no clinical signs in all experimental groups. The topical application of 3$\%$ MXD and HGG in female mice promoted hair regrowth earlier and faster than the control groups. In male mice, the topical application of 3$\%$ MXD and HGG also accelerated hair growth compared with the controls. Ten percent ethanol also promoted hair growth faster than D.W group. The histology of hair growth in experimental groups was strongly associated with the hair regrowth. 3$\%$ MXD and HGG promoted elongation of hair follicles compared with the controls in both female and male mice. Activities of alkaline phosphatase and $\gamma$-glutamyl transpeptidase, enzymes related to hair growth, significantly increased after treatments of 3$\%$ MXD and HGG for 2 weeks in both female and male mice (p < 0.05). These results suggest that HGG has hair growth promoting activities and it can be for treatment for alopecia.

Remediation of Water Quality Using Up-Flow Circulation Apparatus(UFCA) in a Reservoir (용승순환장치를 이용한 호소의 수질개선에 관한 연구)

  • Kang, Chang-Min;Chung, Seon-Yong
    • Journal of Environmental Health Sciences
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    • v.34 no.3
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    • pp.255-260
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    • 2008
  • This study was conducted to analyse the effects of the UFCA for treating polluted water in a reservoir. The UFCA mixes water by circulation of surface and bottom water layers. The circulation supplies oxygen to bottom of the reservoir, resulting in water quality improvement. With a UFCA in use, we surveyed the changes of temperature, pH, transparency, depth, conductivity, DO, COD, BOD, T-N, T-P and Chlorophyll-a for 7 months from Feb. to Aug. in 2004 in our experimental reservoir. There was little difference in the surface and bottom temperatures of the reservoir because of water mixing by the UFCA. However, pH was changed from 7 to 9. The transparency of water was about 80 cm through the all periods. Conductivity was $150\;{\mu}S/cm$ in early Feb., but increased to $270\;{\mu}S/cm$ in early March. Little change was seen in DO with depth, but it was maintained above 6 mg/l in June and July. BOD increased from 2.1 to 12.2 mg/l. The study reservoir did not undergo any eutrophication during the period of our experiment, but the comparison reservoir had an algae-bloom. The COD in the experimental reservoir increased from 5.4 to 14.5 mg/l. The COD concentration of the experimental reservoir was higher than comparison reservoir at the beginning of the study but in August this situation was reversed. SS concentration increased from 13.5 to 23.5 mg/l in Feb., but it fell from between 8.5 to 11.2 mg/l in July. T-N was increased from 1.3 to 4.9 mg/l. It increased up to 3 times in the rainy season as compared to other components. However the comparison reservoir increased up to 40 times higher than the experimental reservoir in the same period. T-P increased from 0.04 to 0.17 mg/l. The ratio of T-N to T-P increased from 20:1 to 40:1 which means that T-P was a growth limiting factor for algae and aquatic plants. Chlorophyll-a increased from 20 to 120 mg/l, and its concentration was correlated with T-P, such that Chlorophyl-a concentration increased with increased of T-P concentration. The concentrations of COD, T-N, T-P and other parameters were higher in the experimental reservoir than in the comparison reservoir but this situation was reversed in July, when the most severe eutophication occurred. The results show that overall the experimental reservoir was greatly remedied by UFCA. The UFCA accelerated the degradation of aquatic organic materials through effective supply of air with up-flow and circulation of water. We conclude that the UFCA can be very effective in aspect of the remediation of water quality incontaminated reservoirs and lakes.

Powdered Wound Dressing Materials Made from wild Silkworm Antheraea pernyi Silk Fibroin on Full-skin Thickness Burn Wounds on Rats

  • Kim, Min-Keun;Yoo, Ki-Yeon;Kwon, Kwang-Jun;Kim, Seong-Gon;Park, Young-Wook;Lee, Kwang-Gill;Jo, You-Young;Kweon, Hae-Yong
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.36 no.3
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    • pp.111-115
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    • 2014
  • Purpose: This study evaluated powdered burn wound dressing materials from wild silkworm fibroin in an animal model. Methods: Fifteen rats were used in this experiment. Full-thickness $2{\times}2cm$ burn wounds were created on the back of rats under anesthesia. In the two experimental groups, the wounds were treated with two different dressing materials made from silkworm fibroin. In the Control Group, natural healing without any dressing material was set as control. The wound surface area was measured at five days, seven days and 14 days. Wound healing was evaluated by histologic analysis. Results: By gross observation, there were no infections or severe inflammations through 14 days post-injury. The differences among groups were statistically significant at seven days and 14 days, postoperatively (P<0.037 and 0.001, respectively). By post hoc test, the defect size was significantly smaller in experimental Group 1 compared with the Control Group and experimental Group 2 at seven days postoperatively (P=0.022 and 0.029, respectively). The difference between Group 1 and Group 2 was statistically significant at 14 days postoperatively (P<0.001). Group 1 and control also differed significantly (P=0.002). Group 1 showed a smaller residual scar than the Control Group and Group 2 at 14 days post-injury. Histologic analysis showed more re-epithelization in Groups 1 and 2 than in the Control Groups. Conclusion: Burn wound healing was accelerated with silk fibroin spun by wild silkworm Antheraea pernyi. There was no atypical inflammation with silk dressing materials. In conclusion, silk dressing materials can be used for treatment of burn wound.