• Title/Summary/Keyword: accelerated experiment

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Effects of Calorie Restriction on Microsomal Mixed Function Oxidase System and Free Radical in Kidney of SAMP8 Mice

  • kim, Hyun-Jeong;Choi, Jin-Ho;Rhee, Soon-Jae
    • Nutritional Sciences
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    • v.7 no.4
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    • pp.189-195
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    • 2004
  • 1bis study investigated the antioxidative effect in kidney of senescence-accelerated prone SAMP8 mice with calorie restriction. 4-weeks-old SAMP8 female mice were divided into 4 groups according to the experimental feeding period: for 4, 8, 12 month, and at natural death. Each group was subdivided into 2 groups, with thirteen mice each one, as ad libitum group and as dietary restriction group (60% of ad libitum feeding amount). After feeding for a given period, the mice were sacrificed to get the following results: among the experimental groups, there wereno significant differences in xanthine oxidase (XOD) activity in their kidney tissues. The contents of cytochrome $P_{450}$ decreased in ad libitum group and dietary restriction group by age. The activity of NADPH-cytochrome $P_{450}$ reductase showed a trend similar to cytochrome $P_{450}$. Superoxide radical content increased with age. At the 4th, 8th and 12 months of the experimental period, the activity in the dietary restriction group was less than that of ad libitum group by as much as 17% 14% and 14% respectively. For hydrogen peroxide, the contents were increased in the ad libitum group with age, while no correlation between content and age was observed in the dietary restriction group. In the 8th and 12th months of the experimental period, the were in the dietary restriction group less than that of ad libitum group counterpart as much as 17% and 20o/c, respectively. For the cellular membrane stability of the kidney, no significant correlation with age was observed in either the dietary restriction group or the ad libitum group. However at the 12th month of the experiment, however, the stability in the dietary restriction group was 11 % higher than that in the ad libitum group. In conclusion, with these results obtained from the SAMP8 mouse model, we demonstrate that dietary restriction has the effects of anti-oxidation and anti-senescence in the kidney.

Dynamic Characteristics of Multiple Bars in the Channels with Erodible Banks (하안침식을 고려한 복렬사주의 동적 거동 특성 분석)

  • Jang, Chang-Lae
    • Journal of Korea Water Resources Association
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    • v.46 no.1
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    • pp.25-34
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    • 2013
  • In this study, the development processes of multiple bars in the channels with erodible banks were investigated by double Fourier analysis. The initially straight channels in the experiment flume were widened with eroding the side banks, and the multiple bars were generated and grew due to stalling of the sediment on the bed. The bars migrated downstream and the size of the bars increased with time. The flow was separated around the bars, and the bed and banks near the bars were scoured due to the impinged secondary flow. The morphologic changes were accelerated by the bank erosion, which affected the fluctuations of sediment discharge downstream. The double Fourier analysis of the bed waves showed that 1-1 mode (alternate bar) was dominant at the initial stage of the channel development. As time increased, 2-3 mode (central or multiple bars) was dominant due to the increased width to depth ratio. Moreover, the number of bars in a cross section of the channel were increased due to the non linearity of alternate bars. The width to depth ratio was increased by the bank erosion, which affected the bar migration and the bar wavelength. However, the dimensionless tractive force was decreased by it.

THE EFFECT OF THE TRICALCIUM PHOSPHATE AND VITAPEX ON THE DOGS' PERIAPICAL TISSUES (Tricalcium phosphate와 Vitapex가 치근단 조직에 미치는 영향에 관한 실험적 연구)

  • Choi, Gi-Won
    • Restorative Dentistry and Endodontics
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    • v.14 no.1
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    • pp.71-84
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    • 1989
  • The purpose of this study was to evaluate the effect of tricalcium phosphate and Vitapex on the dogs' periapical tissues. Twenty mandibular premolars from 5 healthy dogs were used for this study. After the animals were anesthetized intramuscularly, pulp chambers were open and pulp tissue was extirpated with a barbed broach and H-file. Then the working length of the root canal was measured with H-file and pulp tissue was completely removed. Before the actual canal filling, the root canals of twenty teeth have been experimentally infected with opening the pulp chamber for 5 weeks. Periapical radiographs of the experimental teeth were taken to monitor the periapical pathological condition. Each root apex of 20 premolars was perforated with engine reamer and the root canals were enlarged with No. 30-60 H-files. They were divided into treated as follows. Control group: The root canal was filled with gutta-percha. Experimental group 1: The canal was dried with sterile paper points and mixture of tricalcium phosphate and physiological saline was overfilled beyond the root apex with a lentulo spiral. Then the root canal was filled gutta-percha and lateral condensation and the pulp chamber was filled with Caviton. Experimental group 2: The root canals were overfilled with Vitapex and were treated in the same manner as those in experimental group 1 At 1,2,3, and 8 weeks after experiment, the periapical tissues including the alveolar bone were fixed with 10% formalin solution for I week and decalcified with Plank-Rycho solution for 5 weeks. The specimens were embedded in paraffin and serial sections were cut into a thickness of 6 ${\mu}m$ at the plane of the root apex. Hematoxyline-eosin and Masson's trichrome stain were made for the histo-pathological examinations. The results were as follows: 1. Ingrowth of collagen fiber was observed from 1 week in control group and experimental groups. 2. The rate of bone formation of experimental group 1 was accelerated more than that of experimental group 2. 3. Resorption of cementum was seen in control group, but apposition of cementum was seen in experimental groups.

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Influence of Aging of Lead Rubber Bearing on Seismic Performance of Bridges (납고무받침의 노화가 교량의 내진성능에 미치는 영향)

  • Park, Seong-Kyu;Oh, Ju
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.32 no.2A
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    • pp.109-116
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    • 2012
  • The dynamic properties of lead rubber bearings, which are used as isolator, are dependent on the main rubber's dynamic behaviors and nonlinear qualities. Rubber materials tend to undergo an aging process under the influence of mechanical or environmental factors, so they can end up inevitably facing damage. A main cause of such aging is known to be oxidization, which occurs through the heat of reaction at high temperatures. Accordingly, in this study an accelerated thermal aging test was carried out in order to compare the characteristic values of the bearings with each other before and after thermal aging occurs. As a result of this experiment, it was found that a thermal aging phenomenon could have an effect on shear stiffness, energy absorption, and equivalent damping coefficients. Furthermore, a decline in the dynamic properties of the lead rubber bearings by means of the thermal aging process was applied to an actual bridge and the effects of such thermal aging on the seismic performance of the bridge were also compared and analyzed based on numerical analysis. As a result of this analysis, it was found that the changes in the basic properties of the lead rubber bearings have a minor effect on the seismic performance of bridges.

Application of ASTM C 1260 for Cement Matrix Mixed with FlyAsh and Lithium Nitrate (플라이애시와 질산리튬을 사용한 시멘트 경화체의 ASTM C 1260 적용성 검토)

  • Kim, Seong-Kwon;Yun, Kyong-Ku;Hong, Seung-Ho;Kang, Moon-Sik
    • International Journal of Highway Engineering
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    • v.14 no.3
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    • pp.1-7
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    • 2012
  • The purpose of this study was to review application of ASTM C 1260 for cement matrix with flyash and lithium nitrate using reactive aggregate. The experimental program included the accelerated mortar bar test (AMBT: ASTM C 1260) for the slate which was evaluated as reactive aggregate by ASTM C 1260 at the previous study. The cement, which was substituted by 10, 20, 30% flyash containing less than 10% CaO, could control ASR expansion. From the experiment applying lithium nitrate to control ASR, the mortar bar containing lithium nitrate showed more than 0.1% expansion at 14 days. This is probably due to dissolution of lithium nitrate in NaOH solution during test periods. Thus, it is necessary to adopt another test method to verify the control effect of lithium nitrate against alkali-silica reaction.

Influence of Current Density Application Time on the Corrosion Damage of Offshore Wind Steel Substructure in Galvanostatic Corrosion Experiment (해상풍력 하부 구조물용 강재의 정전류 부식 시험 시 전류밀도 인가 시간이 부식손상에 미치는 영향)

  • Lee, Jung-Hyung;Park, Jae-Cheul;Han, Min-Su;Jang, Seok-Ki;Kim, Seong-Jong
    • Journal of the Korean institute of surface engineering
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    • v.49 no.5
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    • pp.431-438
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    • 2016
  • This research investigated the relationship between the corrosion damage characteristics of offshore wind steel substructure and the time of current density application by electrochemical accelerated short-term corrosion test. The galvanostatic corrosion was conducted on the steel specimens in natural seawater with a constant current density ranging from $1mA/cm^2$ to $200mA/cm^2$ for 1 ~ 180 min. Macro and micro observation was carried out on the surface of the corrosion damaged area using SEM and 3-dimensional analysis microscope. The weight loss of the specimens before and after was calculated as the difference between the initial weight prior to corrosion and weight after removal of the corrosion product. It was shown that during galvanostaic corrosion process, the corrosion behavior could be characterized by the onset of pitting corrosion in the early stage and the uniform corrosion in the late stage, showing damage development in the depth direction with the time of current application. The result of the 3D analysis revealed that both damage depth and surface roughness increased with increasing time of current application. The weight loss curves with time showed that a coefficient of determination ($R^2$) was relatively high for the relationship between the time of current application and weight loss. As a result, the degree of corrosion can be controlled by simply varying the time of current application.

Effects of Root Head Diameter and Fertilization on Shoot Growth and Root Yield in Angelica gigas NAKAI (근두경의 크기와 시비가 참당귀의 생육 및 수량에 미치는 영향)

  • 조선행;김기준
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.3
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    • pp.254-258
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    • 1991
  • Experiment was conducted to investigate the effects of size of root crown at transplanting and fertilization on the growth and root yield of Angelica gigas Nakai. Plant height and fresh weight of shoots were increased by fertilizer application, but was not different in emergence rate. Size of root crown at transplanting was also affected the growth of shoot. Plant which had 7.2mm of root crown at transplanting showed highest in plant height and fresh weight of shoots. Growth of root in terms of root length, number of root branches, diameter of root crown, and fresh weight of root was increased by fertilizer application and 5.4mm of root crown at transplanting. Application of fertilizer accelerated the development and growth of node on the stem, which in turn resulted bolting and flowering. Plants which had greater than 7.2mm of root crown at transplanting and application of fertilizer were produced woody roots. Woody roots cannot be used for medicinal purpose. Therefore, yield of roots was highest at transplanting of 3.lmm root crown with fertilizer application.

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Effects of Foliar Spray of Calcium Salts and Ethephon on the Fruit Quality of Satsuma Mandarin (Citrus unshiu Marc. Miyagawa wase) in the Plastic Greenhouse (Ethephon과 Ca제제 혼용 엽면살포에 의한 플라스틱하우스에서 재배된 온주밀감의 품질향상 효과)

  • 김용호;한승갑
    • Journal of Bio-Environment Control
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    • v.10 no.2
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    • pp.118-119
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    • 2001
  • This experiment was carried out to examine the effects of foliar applications of 100 mg.L$^{-1}$ ethephon mixed with different calcium formulae (10,000 mg.L$^{-1}$ Clef-non, 2,000 mg.L$^{-1}$ Cell-bine, 2,000 mg.L$^{-1}$ Hicalux, and 2,000 mg.L$^{-1}$ calcium acetate monohydrate) on the rind coloration, defoliation and fruit quality of satsuma mandarin cultivated in the plastic greenhouse. Foliar applications of ethephon alone or in mixture with either Clef-non, Cell-bine, Hicalux or calcium acetate monohydrate demonstrated that increased 'a'value of peel chromaticity as compared to the control. The treatments encouraged fruit colour and allowed the harvest date to be predicted. The defoliation ratio decreased considerably by foliar applications of ethephon mixed with Cell-bine or Hicalux. The sugar content of fruit increased by 1.6, 1.54, and 1.54$^{\circ}$Bx with the foliar applications of ethephon, ethephon+Clef-non, and ethephon+Cell-bine, respectively. With the foliar applications of ethephon in mixture with Cell-bine, fruit coloration was accelerated. defoliation was delayed, and fruit quality was improved.

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Biodegradation of the Commercial Phenoxy Herbicide 2,4-D by Microbial Consortium (미생물 컨소시엄에 의한 시판 페녹시계 제초제 2,4-D의 생물분해)

  • 오계헌;김용석
    • KSBB Journal
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    • v.9 no.5
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    • pp.469-474
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    • 1994
  • The purpose of the work was to evaluate the feasibility of a biological treatment process for the phenoxy alkanoic herbicide 2,4-D(2,4-dichlorophenoxyacetic acid) as a commercial pesticide. The phenoxy herbicide was 2,4-D amine salts which contained 40%(vol/vol) 2,4-D and 60%(vol/vol) solvent. A microbial consortium has been derived by enrichment with 2,4-D. The consortium utilized 2,4-D as the sole source of carbon and energy. Optimal pH on the 2,4-D degradation was 7.0 in this experiment. As concentrations of 2,4-D were increased, the degradation by microbial consontium became inhibited. The amendment with yeast extract and ascorbic acid accelerated the degradation of 2,4-D. High performance liquid chromatography methodology was used to measure 2,4-D and it also resolved 2,4-DCP(2,4-dichlorophenol), the corresponding phenol as intermediate. Gas chromatography-mass spectrometry was used for preliminary identification of the intermediate 2,4-DCP. UV scans of spent cultures showed that the maximum absorption of 2,4-D at the wavelength of 283 nm was decreased toward the end of incubation, but the consortium displayed no detectable spectral changes or peak shifts in the UV absorbance.

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Propeller Wake Measurement of a Model Ship in Self Propulsion Condition using Towed Underwater PIV (입자영상유속계를 이용한 자항상태 모형선의 프로펠러 후류 계측)

  • Seo, Jeonghwa;Yoo, Geuk Sang;Lim, Tae Gu;Seol, Dong Myung;Han, Bum Woo;Rhee, Shin Hyung
    • Journal of the Society of Naval Architects of Korea
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    • v.51 no.2
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    • pp.171-177
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    • 2014
  • A two-dimensional particle image velocimetry (2D PIV) system in a towing tank is employed to measure a wake field of a very large crude oil carrier model with rotating propeller in self propulsion condition, to identify characteristics of wake of a propeller working behind a ship. Phase-averaged and time-averaged flow fields are measured for a horizontal plane. Scale ratio of the model ship is 1/100 and Froude number is 0.142. By phase-averaging technique, trajectories of tip vortex and hub vortex are identified and characteristic secondary vortex distribution is observed in the hub vortex region. Propeller wake on the starboard side is more accelerated than that on the port side, due to the difference of inflow of propeller blades. The hub vortex trajectory tends to face the port side. With the fluctuation part of the phase-averaged velocity field, turbulent kinetic energy (TKE) is also derived. In the center of tip vortex and hub vortex region, high TKE concentration is observed. In addition, a time-averaged vector field is also measured and compared with phase-averaged vector field.