• Journal of Animal Science and Technology
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• v.50 no.6
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• pp.807-818
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• 2008

This study was conducted to prove the optimum feeding method of Lactobacillus in broiler chickens by investigating the intestinal viability of ingested Lactobacillus and the effect of feeding levels and frequency of Lactobacillus on growth performance in broiler chickens. In experiment 1, A total of one hundred, 5 weeks old male broiler chickens(Abor Acre) were fed Lactobacillus reuteri avibro2 expressed green fluorescent protein(GFP) at 104cfu/g diet to investigate the retention time of ingested Lactobacillus in the intestine for 1 day. The percentage of Lactobacillus expressed GFP in intestinal contents was 26% at 1 day after fed Lactobacillus expressed GFP. The percentage of Lactobacillus expressed GFP in intestinal contents was decreased in length of time. In experiment 2, A total of four hundred eighty, 1-d-old male broiler chicks(Abor Acre) were randomly divided into 4 groups with 4 replicates of 30 birds each to prove the optimum feeding level of Lactobacillus. The treatments were control(free antibiotics), Lactobacillus reuteri avibro2 5.0×10cfu/mL, 5.0×103cfu/mL, and 5.0×105cfu/mL. The final body weight and body wight gain of Lactobacillus reuteri avibro2 5.0×103cfu/mL were the highest in all groups(P<0.05). Feed conversion ratio was not significantly difference among the groups. The number of intestinal lactic acid bacteria in Lactobacillus treated groups tended to be improved or significantly increased as compared to that of control(P<0.05). Protein and fat digestibility in Lactobacillus 5.0×103cfu/mL and 5.0×105cfu/mL treated groups were significantly improved(P<0.05). No significant differences were observed on the availability of dry matter and crude ash in Lactobacillus treatments compared to those of control. In experiment 3, A total of six hundred 1-d-old male broiler chicks(Abor Acre) were randomly divided into 4 groups with 4 replicates of 30 birds each and were fed Lactobacillus reuteri avibro2 at intervals of 1, 2, 3, and 5 day for five weeks. Feeding level of Lactobacillus was 5.0×103cfu/mL The final body weight and body wight gain of Lactobacillus reuteri avibro2 5.0×103cfu/mL were the highest in all groups(P<0.05). The final body weight and body weight gain were significantly increased, when Lactobacillus was fed at intervals of 1 days, or 2 days. There were no significant differences in feed intake and feed conversion ratio among the all groups. The number of intestinal lactic acid bacteria in Lactobacillus treated groups tended to be improved or significantly increased as compared to that of control(P<0.05). No significant differences were observed on the number of coliform bacteria and Salmonella of ileum and cecum. Consequently, supplemental Lactobacillus influenced positive effects on the growth performance, nutrient availability and intestinal microflora. The optimum feeding level of Lactobacillus was 5.0×103cfu/mL, and the constant feeding of Lactobacillus was effective.

• Korean Journal of Poultry Science
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• v.28 no.1
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• pp.15-25
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• 2001

This experiment was conducted to investigate the effect of feeding two strains of Lactobacillus and virginiamycin on performance, nutrients digestibility and intestinal microflora of broiler chicks(Abor acres$\times$Abor Acres) were randomly allocated into six treatments with four replications for five weeks. Control(no supplement), 0.05% virginiamycin(VM), Lactobacillus crispatus avibro1(LC), Lactobacillus reuteri avibro2(LR), LC+0.05% VM(LC+VM), LR+0.05% VM(LR+VM) were supplemented into basal diets, which contained ME 3,100kcal/kg and CP 22.0, 20.0% for starting and finishing period, respectively. Weight gain, feed intake and feed conversion(FC) were weekly measured. Nutrients digestibility, intestinal microflora and fecal noxious gas were examined at the end of experiment. Weight gains of chicks fed Lactobacillus or VM was significantly higher than control(P〈0.05). Feed intake increased significantly in those supplemental groups(P〈0.05). FC of chicks fed Lactobacillus or VM significantly lower than control(P〈0.05). Degestibility of crude protein, calcium, and phosphorus improved significantly in alone or combined Lactobacillus treatments(P〈0.05). Whereas DM, crude fat and ash digestibility were not statistically different. Feeding Lactobacilli tended to increase the total Lactobacillus spp. in ileum at one and three weeks of age(WOA) and showed significantly higher in cecum than control at 5 WOA. Total yeast were not shown difference at 1 and 3 WOA, but significantly increased at 5 WOA(P〈0.05). The ileal and cecal anaerobes were started to increase from the first WOA. Fecal NH$_3$gas tended to decrease in Lactobacillus treatments compared to that of other treatments.

• Korean Journal of Poultry Science
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• v.30 no.3
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• pp.151-159
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• 2003

Two experiments were carried out to evaluate the effects of feeding Aspergillus oryzae(AO) ferment on performance, intestinal microflora, serum components, ammonia generation and litter dampness in broiler chicks. In experiment I, three hundred sixty, one day old broiler chicks, Abor Acres, were fed 0 and 0.1% of Aspergiilus oryzae short conidia ferment(AOS) and 0.1% of Aspergillus oryzae long conidia ferment(AOL) for five weeks. In experiment II, three hundred sixty, one day old broiler chicks, Abor Acres were fed 0, 0.1 and 0.2% of Aspergillus oryzae long conidia ferment(AOL) for five weeks. In experiment I, growth rates were not statistically different among dietary treatments. AOS and AOL showed increased tendency in weight gain and feed intake compared to those of control, whereas feed conversion was not different. Litter dampness of AOS and AOL was also tended to decrease compared to that of control, but was not significantly different. Fecal ammonia gas generation was decreased in feeding AOS and AOL, and maintained 1/2 to 3/4 compared to the control. In serum metabolites, AOS and AOL increased glucose and calcium, and decreased total protein, blood urea nitrogen and total cholesterol. In experiment II, body weight of chicks fed 0.1 and 0.2% AOL were heavier than the control(P<0.05). Feed intake of chicks fed 0.1 and 0.2% AOL also were higher than the none, but feed conversion ratio was not different among treatments. Ileal and cecal microflora showed increased tendency in lactic acid bacteria compared to those of the control. Salmonella and E. coli were decreased in ileum of chicks fed 0.1 and 0.2% AOL. In conclusion, feeding AO ferment increased growth performance and improved intestinal microflora of broiler chicks and environments of broiler house.

• Korean Journal of Poultry Science
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• v.25 no.2
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• pp.91-98
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• 1998

In order to compare economics of soybean meals (SBMS) imported from Brazil and the U.S., a feeding trial was conducted with 480 commercial broiler chicks (Abor Acres strain) for 38 days. The Brazilian SBM was brownish in color and the protein solubility in 0.2 % KOH solution was 63. 4 %. The U.S. SBM, on the other hand, was a dehulled SBM, and uniform in color (light yellow) and particle size. The protein solubility value of the latter was 76.6 %. Two dietary protein levels (19 % and 21 %) were used for each source of SBM (2x2 factorial). Chicks fed diets containing the U.S. SBM grew significantly faster (P<0.05) and utilized feed more efficiently than chicks fed diets containing Brazilian SBM. Chicks fed diets containing 21 % CP showed better growth performance and profitability than those fed the 19 % CP diets. The price of the U.S. SBM was higher but the feed cost per unit gain was lower than the Brazilian SBM. Results suggest that there are over-cooked SBMs being traded on the Korean rnarket, and attention should be given to not only the price but also the quality of SBMs.

• Korean Journal of Poultry Science
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• v.23 no.3
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• pp.129-134
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• 1996

his study was conducted to determine the effect of dietary garlic supplementation on the growing performance and activity of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase in broiler chicks from 3 to 5 wk post hatching. Fifty chicks were divided into 5 groups with 10 replicates per treatment and placed in a wire battery cage. Five levels of dietary garlic(0, 0.1, 0.3, 0.6 and 1.0%) were provided in an one way analysis. Feed and water were given ad libitum. Feed intake, weight gain and feed conversion rate(FCR) were not affected by the garlic supplementations. The HMG-CoA reductase activity decreased significantly(P<0.05) with the supplementation of garlic powder, compared to the garlic free group. As the dietary garlic level was increased, chicks showed decreased lipid contents in liver and blood serum. The results of this study indicate that blood cholesterol of chicks fed garlic supplemented diet might be reduced by inhibition of RMG-CoA reductase activity.

• Korean Journal of Veterinary Service
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• v.30 no.3
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• pp.329-338
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• 2007

Samples collected from 15 broiler farms(47 flocks, 920 1-day-old chicks) during March to December, 2006, To survey serum antibody titers of NDV, IBDV and MG/MS, the antibodies of ND viruses were detected by HI test and ELISA, against antibodies of IBD viruses and MG/MS by ELISA. The antibody titers of NDV showed 6.4, HI and 6,968, ELISA, respectively. The rate to below protective antibody levels(${\ge}5$, HI and ${\ge}1,000$, ELISA) were 8%, HI, 5%, ELISA, specially, Baeksemi were 22%, HI, 14%, ELISA. The rate of positive by ELISA showed 99%(914/920). The ELISA titer of IBDV showed mean titer 3,890. The rate of positive were 93% (857/920), specially, Baeksemi were 84%. The ELISA titers of MG/MS showed mean titer 5,666. The rate of positive were 78% (715/920) and 100%, Abor-Acre, 97%, Baeksemi, respectively. The antibodies not detected from 18%, ELISA titers was varied from 500 to 20,000. At antimicrobial susceptibility of E coli, Staphylococcus spp and Salmonella spp isolated from 1-day-old chicks, E coli were susceptible to AmC, AM, NOR, SXT, ENR, CIP, Staphylococcus spp were susceptible to AmC, SXT, AM, ENR and Salmonella spp were susceptible to AM, AmC, SXT and P.

• Korean Journal of Poultry Science
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• v.14 no.2
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• pp.103-108
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• 1987

This study was conducted to bioassay of amino acid availability of feather meal processed by a different methods, commercial feather meal and raw feather meal, The feather meals were processed by labolatory pressure cooker(autoclave) at 2kg/$\textrm{cm}^2$ for 30 minutes ; 3kg/$\textrm{cm}^2$ for 90 minutes : 4kg/$\textrm{cm}^2$ for 120 minutes. Chick employed in the present experiment were Abor Acre strain, male or meat type (body weight, 100-140g), fed with semipurified diet and protein free diet was given during the determination of the metabolic and endogeneous amino acid. The contents of amino acid of samples were investigated by ion-exchange chromatography. The results were as follows; 1. The amino acid availability of raw, 2kg/$\textrm{cm}^2$ for 30 minutes, commercial, 4kg/$\textrm{cm}^2$ for 120 minutes and 3kg/$\textrm{cm}^2$ for 90 minutes of feather meal were -3.09, 63.28, 67.47, 71.22 and 73.75% respectively. 2. The essential amino acid availability of raw, 2kg/$\textrm{cm}^2$ for 30 minutes, commercial, 4kg/$\textrm{cm}^2$ for 120 minutes and 3kg/$\textrm{cm}^2$ for 90 minutes of feather meal were 2.55, 66.78, 66.89, 72,56 and 73.62%, respectively. 3. In individual amino acid of the different processing loather meal and commercial feather meal, biovailabilities were increased methionine, phenylalanine, leucine, arginine, threonine, isoleucine, however, histidine, lysine and aspartic acid were remarkely decreased. In conclusion, the bioavailability or amino acid for the feather meal processed at 3kg/$\textrm{cm}^2$ for 90 minutes was superior to those of other treatment or raw feather meal.

• Korean Journal of Poultry Science
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• v.16 no.3
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• pp.149-156
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• 1989

Two broiler experiments were carried out to determine whether supplementation of zeolite in the diet would affect broiler performance and economical efficiency. Day old male broiler chickens of Abor Acres strain were used. In the first experiment,60 birds were distributed into f groups of 10 birds each. Three groups of chickens were randomly placed on one of the following single-stage (0-6wks) experimental diets； control diet (non-supplemented) or 2% zeolite supplemented diet (supplemented to the control). In the second experiment,216 birds were distributed into 24 groups of 9 birds each. Eight groups of chickens were randomly placed on one of the 3 two-stage diet (starter；0-4wks, finisher；5-7wks)；control diet ($T_1$ , non-supplemented), 2% zeolite diet ($T_2$：isocaloric and isonitrogenous as $T_1$ ) and 2% zeolite supplemented diet ($T_3$, supplemented to the control). Birds were housed in the batteries, and feeds and water were fed ad libitum. The results of Experiment 1 showed that birds fed control diet were heavier than those fed 2% zeolite supplemented diet up to 4th wks of age. However, birds fed the latter grew more at 5th and 6th wk. Overall weight gain, feed intake and feed efficiency were not significantly different. The results of Experiment 2 showed that there were no significant differences in weight gains, feed intakes and feed efficiencies among treatments during starter, finisher to overall period. The moisture levels in excreta of the birds were significantly (P＜0.01) less when zeolite was included in the diets and those in excreta significantly (P ＜ 0.01) increased as the ambient temperature increased. Economical efficiency expressed in fed cost (won) required per kg weight gin were 346.55 (T$_3$),348.34 ($T_1$ ) and 359.85 ($T_2$).

• Journal of the Korean Society of Food Science and Nutrition
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• v.12 no.4
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• pp.395-400
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• 1983

The experiment was conducted to study the effect of protein level on weight gain, feed consumption and the efficiencies of feed, energy and protein when the broiler chick was fed the diet of different protein level with the iso-calorie content. The energy and protein level of the basal ration was 3200 kcal and 22.85% for starter period and 3265 Kcal and 18.58% for finisher period, respectively. In order to increase the protein level of the experimental diet, the basal diet was substituted by fish meal by the ratio of 3, 6, 9, and 12%. The male Abor Acre broiler chicks were employed for 8 weeks and the result obtained were as follows. 1. The weekly body weight gain was significantly greater for Diet C than for Diet A and E in the starter period and greater for Diet A than for Diet E in the finisher period (P<0.05). 2. In stater period the feed intake was not affected by the protein level but in finisher period the feed intake for Diet C, D, and E was significantly reduced compared with Diet A. 3. As for feed conversion ratio, Diet C of 1.43 was improved significantly compared with Diet A of 1.58(P<0.05). 4. As the protein level of diet increased, the energy efficiency ratio increased and however, the protein efficiency ratio decreased in starter and finisher period. In conclusion the best performance of broiler is thought to be realized when the protein and energy level was 23% and 3200 Kcal for starter period and 20% and 3250 Kcal for finisher period, respectively.

• Asian Journal of Turfgrass Science
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• v.24 no.2
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• pp.145-148
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• 2010

Nitrogen (N) is one of the most important nutrients among 17 essential nutrients for maintaining turfgrass color and quality. The slow release fertilizers were initially developed to provide a more consistent release of nitrogen over a longer period and are often used to decrease leaching potential from sandy soils. The goal of this study is to determine if various slow release N sources affect the rate at which turfgrass establishes. Six nitrogen sources were evaluated; Nitroform (38-0-0), Nutralene (40-0-0), Organiform (30-0-0), Sulfur coated urea (SCU, 37-0-0), urea (46-0- 0), and Milorganite (6-0-0). The root zone media was seeded and sodded with 'Limousine' Kentucky bluegrass (Poa pratensis L.). Sodded pots produced 182 to 518 g more clipping dry weight than seeded pots. Among seeded pots, Milorganite produced greater amount of root dry weight than any other N sources. Because the period of turfgrass growth is different between sodded and seeded plots, there were differences on clipping yield and root growth. Overall, high N rate had turf color greater than acceptable color of 6 among seeded pots throughout the study. However, low N rate didn't produce acceptable turf color throughout the study. Based on the result of this tudy, ilorganite would be ecommended for new establishment of Kentucky bluegrass an urea with less clipping yield which can lead to reduce abor.