• Molecules and Cells
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• v.37 no.4
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• pp.330-336
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• 2014

Thymosin beta4 (TB4) has multiple functions in cellular response in processes as diverse as embryonic organ development and the pathogeneses of disease, especially those associated with cardiac coronary vessels. However, the specific roles played by TB4 during heart valve development in vertebrates are largely unknown. Here, we identified a novel function of TB4 in endothelial-mesenchymal transformation (EMT) in cardiac valve endocardial cushions in zebrafish. The expressions of thymosin family members in developing zebrafish embryos were determined by whole mount in situ hybridization. Of the thymosin family members only zTB4 was expressed in the developing heart region. Cardiac valve development at 48 h post fertilization was defected in zebrafish TB4 (zTB4) morpholino-injected embryos (morphants). In zTB4 morphants, abnormal linear heart tube development was observed. The expressions of bone morphogenetic protein (BMP) 4, notch1b, and hyaluronic acid synthase (HAS) 2 genes were also markedly reduced in atrio-ventricular canal (AVC). Endocardial cells in the AVC region were stained with anti-Zn5 antibody reactive against Dm-grasp (an EMT marker) to observe EMT in developing cardiac valves in zTB4 morphants. EMT marker expression in valve endothelial cells was confirmed after transfection with TB4 siRNA in the presence of transforming growth factor ${\beta}$ ($TGF{\beta}$) by RT-PCR and immunofluorescent assay. Zn5-positive endocardial AVC cells were not observed in zTB4 morphants, and knockdown of TB4 suppressed TGF-${\beta}$-induced EMT in ovine valve endothelial cells. Taken together, our results demonstrate that TB4 plays a pivotal role in cardiac valve formation by increasing EMT.

• Journal of the Korea Convergence Society
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• v.8 no.9
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• pp.339-345
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• 2017

Recently, agriculture and the environment has changed dramatically due to global warming and abnormal weather. In particular, it is necessary to develop new agricultural techniques according to transforming the growing environment of agricultural crops. Therefore, "Smart Farm" building technology for controlling agricultural environment and improving efficiency for ICT technology development has recently been introduced. However, in reality, systematic and objective evaluation items are absent at various levels and management levels that affect the management environment of the smart farm. In this research, it derived the importance index among the factors associated with Smart Farm technology by AHP method. As a result, in order to evaluate comprehensive operation and management of the smart farm, the two evaluation fields(sensor device and control/information management system) were selected as the top evaluation items. These results mean that system that can detect the growth environment information of agricultural crops and control the growing environment is more important than anything, when smart farm is applied. It is judged that the results of this research can be used as basic data for making evaluation indicators associated with the introduction of smart palm technology in the future.

• KIPS Transactions on Computer and Communication Systems
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• v.2 no.8
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• pp.335-342
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• 2013

Nowadays, the traffic type and behavior are extremely diverse due to the growth of network speed and the appearance of various services on Internet. For efficient network operation and management, the importance of application-level traffic identification is more and more increasing in the area of traffic analysis. In recent years traffic identification methodology using statistical features of traffic flow has been broadly studied. However, there are several problems to be considered in the identification methodology base on statistical features of flow to improve the analysis accuracy. In this paper, we recognize these problems by analyzing the ground-truth traffic and propose the solution of these problems. The four problems considered in this paper are the distance measurement of features, the selection of the representative value of features, the abnormal behavior of TCP sessions, and the weight assignment to the feature. The proposed solutions were verified by showing the performance improvement through experiments in campus network.

• Neonatal Medicine
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• v.15 no.1
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• pp.89-93
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• 2008

Duplication of chromosome 12p has been rarely reported and are thought to be associated with congenital malformations and impaired development. We report a baby boy born with multiple dysmorphic features and congenital malformations. His karyotype was 46,XY, add(12)(p13.3). He has suffered from intrauterine growth restriction at birth. He showed abnormal cranio-facial findings such as microcephaly, hypognathia, clepft palate and low set ear. He presented with absence of uvula, micropenis and rocker bottom features of both feet, congenital heart disease, poor corticomedullary differentiation of kidney, and sensorineuronal hearing loss. We have been follow up him for seizure disorder and delayed development at out patient department.

• Korean Journal of Plant Tissue Culture
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• v.27 no.3
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• pp.175-180
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• 2000

Cotyledonary somatic embryos after being cultured in a liquid MS medium for 1 week were subcultured on a solid MS medium and then the embryos germinated at a rate of 92%, but the rate was lowered by extending the culture period of the embryos on a liquid medium: 26% germination on a liquid medium culture for 4 weeks. Somatic embryos subcultured on the liquid medium showed the normal elongation of hypocotyl and radicle but in part showed secondary embryogenesis on hypocotyl and callus formation on and around the root-hypocotyl juncture. Through observation of scanning electron microscope, apical meristem in plumule showed the loose arrangement of cells, and abnormal leaf primordium formation and growth arrest of the primordium or no leaf primordium formation. Therefore, it is suggested that the germination arrest of carrot somatic embryos on liquid medium culture is due to the structural abnormality of the apical meristem in plumule.

• Molecular & Cellular Toxicology
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• v.2 no.1
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• pp.21-28
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• 2006

Gadolinium chloride ($GdCl_{3}$) was known to block Kupffer cells and generally its toxicity study based on blocking these cells. Therefore, $GdCl_{3}$ frequently used to study toxic mechanisms of hepatotoxicants inducing injury through Kupffer cells. We also tried to investigate the effect of $GdCl_{3}\;on\;CCl_{4}$ toxicity, typical hepatotoxicants. Administration of $GdCl_{3}$ to mice significantly suppressed AST (asparatate amino transferase), ALT (alanine amino transferase) levels which were increased by $CCl_{4}$ treatment. However, $GdCl_{3}$ didn't inhibit the phagocytotic activity of Kupffer cells. Malondialdehyde (MDA) is a good indicator of the degree of lipid peroxidation. In this study, MDA increased by $GdCl_{3}$ administration not by $CCl_{4}$. To understand the toxicity of $GdCl_{3}$, we analyzed global gene expression profile of mice liver after acute $GdCl_{3}$ injection. Four hundred fifty two genes were differentially expressed with more than 2-fold in at least one time point among 3 hr, 6 hr, and 24 hr. Several genes involved in fibrogenesis regulation. Several types of pro-collagens (Col1a2, Col5a2, Col6a3, and Col13a1) and tissue inhibitor of metal-loproteinase1 (TIMP1) were up regulated during all the time points. Genes related to growth factors, chemokines, and oxidative stress, which were known to control fibrogenesis, were significantly changed. In addition, $GdCl_{3}$ induced abnormal regulation between lipid synthesis and degradation related genes. These data will provide the information about influence of $GdCl_{3}$ to hepatotoxicity.

• Journal of Climate Change Research
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• v.7 no.3
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• pp.315-324
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• 2016

The relevant ministries, including the Ministry of Environment in Korea, provided Post-2020 Long-term Mitigation Target and Implementation Plan. The plan consisted of four Business As Usual (BAU) reduction levels by 14.7%, 19.2%, 25.7%, and 31.3% until 2030. The Korean government finalized the mitigation target of 37%. But all the initial alternatives were below the goal, 30% from BAU, that has been promised to the international community as well as set out in the Framework Act on Low Carbon Green Growth. In order to achieve a specific goal, performance management should pursue "Justify doing the right things." Otherwise, performance management would not work properly. According to Kingdon's Policy Stream Framework, abnormal alternatives are difficult to be presented as scenarios because alternative building should focus on the role of the need to adhere to the basic principles and professionals. Such a result is possible only when the policy actors does not balance themselves. Performance management statistics has been analyzed by 6 years CCPI data since 2011, taking into account the impact after enactment. This study also has been complemented by a variety of sources, including the media, documents, and artifacts during the period. As a result, raising awareness about climate change was analyzed as one of the solutions because the climate change issue affects the normal performance management throughout the life of the people to stay linked to the environment.

• Journal of Animal Science and Technology
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• v.63 no.4
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• pp.759-765
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• 2021

Recently, summer temperatures have frequently been abnormal in Korea owing to global warming. In summer, a decrease in feed intake rate and biological activity were observed in Hanwoo (Korean Native Cattle), leading to lower production rates in the industry. However, the precise scale of damage was not reported as with other animals of economic value. This study was conducted to investigate the effects of birth season on birth weight in Hanwoo. Data were collected from 100 local breeding farms from 2016 to 2019. A total of 41,081 Hanwoo calves were classified and analyzed by sex, year, month, and season (March-May, spring; June-August, summer; September-November, fall; and December-February, winter) of birth. The birth weight of Hanwoo calves differed according to birth month. The average birth weight of male calves was 30.47 kg and that of female calves was 28.16 kg. Hanwoo birth weight was the highest in March-born calves and the lowest in July-born calves. The birth weights of calves born in February, March, April, November, and December were significantly larger than those of calves born in July. In addition, the birth weight of Hanwoo calves from the summer was significantly lower than that of calves born in other seasons. Furthermore, Hanwoo steer slaughter age showed a negative correlation, whereas carcass weight had a positive correlation with birth weight. In the beef cattle industry, birth weight is a very important economic characteristic that is related to growth rate. These data will contribute toward planning the reproduction of Hanwoo and analysis of changes in characteristics of economic value owing to high temperatures.

• Korean Journal of Agricultural Science
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• v.46 no.1
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• pp.113-124
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• 2019

Insect-resistant transgenic (Bt-9) rice was generated by inserting mCry1Ac1, a modified gene from the soil bacterium Bacillus thuringiensis, into the genome of a conventional variety of rice (Ilmi). With regard to potential problems such as safety, an evaluation of non-target organisms is necessary as an essential element of an environmental risk assessment of genetically modified (GM) crops. We studied the effects of the Bt-9 rice on the survival of cantor Daphnia magna, a commonly used model organism in ecotoxicological studies. D. magna fed on the Bt-transgenic rice (Bt-9) and its near non-GM counterparts (Ilmi) grown in the same environment (a 100% ground rice suspension). The Bt-9 rice was confirmed to have the inserted T-DNA and protein expression evident by the PCR and ELISA analyses. The feeding study showed a similar cumulative immobility and abnormal response of the Daphnia magna between the Bt-9 rice and Ilmi. Additionally, the 48 h-EC50 values of the Bt-9 and Ilmi rice were 4,400 mg/L (95% confidence limits: 3861.01 - 5015.01 mg/L) and 5,564 mg/L (95% confidence limits: 4780.03 - 6476.93 mg/L), respectively. The rice NOEC (No observed effect concentration) value for D. magna was suggested to be 1,620 mg/L. We conclude that the tested Bt-9 and Ilmi have a similar cumulative immobility for D. magna, a widely used model organism, and the growth of Bt-9 did not affect non-target insects.

• Advances in nano research
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• v.10 no.4
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• pp.359-371
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• 2021

A tumor immune interaction is a main topic of interest in the last couple of decades because majority of human population suffered by tumor, formed by the abnormal growth of cells and is continuously interacted with the immune system. Because of its wide range of applications, many researchers have modeled this tumor immune interaction in the form of ordinary, delay and fractional order differential equations as the majority of biological models have a long range temporal memory. So in the present work, tumor immune interaction in fractional form provides an excellent tool for the description of memory and hereditary properties of inter and intra cells. So the interaction between effector-cells, tumor cells and interleukin-2 (IL-2) are modeled by using the definition of Caputo fractional order derivative that provides the system with long-time memory and gives extra degree of freedom. Moreover, in order to achieve more efficient computational results of fractional-order system, a discretization process is performed to obtain its discrete counterpart. Furthermore, existence and local stability of fixed points are investigated for discrete model. Moreover, it is proved that two types of bifurcations such as Neimark-Sacker and flip bifurcations are studied. Finally, numerical examples are presented to support our analytical results.