• Clinical and Experimental Pediatrics
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• v.46 no.10
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• pp.1032-1035
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• 2003

In pseudohypoparathyroidism as reported by Albright in 1942, the parathyroid gland can normally synthesize and secrete parathyroid hormone(PTH). Pseudohypoparathyroidism has a similar biochemical finding with hypoparathyroidisms like hypocalcemia and hyperphosphatemia due to target tissue resistance to PTH. Administered PTH does not raise the serum levels of calcium and urinary phosphate. PTH activates G-protein in peripheral tissue and adenylate cyclase through a second messenger, cAMP. Pseudohypoparathyroidism produces hyperphosphatemia and hypocalcemia because of the resistance to PTH in peripheral tissue due to a defect of G-protein, although it releases PTH normally. According to the mechanism of resistance, pseudohypoparathyroidism is classified into types : Ia, Ib, Ic and psedopseudohypoparathyroism. Type Ia is accompanied by congenital growth retardation and abnormal bony development that shows mental retardation, obesity, low height, round face, short metacarpal bone and metatarsal bone, ectopic calcification, etc. We report a case of pseudohypoparathyroidism in a premature who shows hypocalcemia, hyperphosphatemia, elevation of serum PTH and 24 hr urinary basal c-AMP in biochemical tests without Albright's hereditary osteodystrophy at physical examination, accompanied by a spontaneous fracture in the femur.

• Journal of Plant Biotechnology
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• v.5 no.1
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• pp.19-23
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• 2003

The Ac (activator) which is one of the well-characterized transposable elements from maize was examined for its transposition possibility to the heterologous plant (P.nigra x maximowiczii) genome via Agrobacterium tumefacience (LBA4404) mediated transformation system. A number of transgenic plants were successfully recovered after 30 weeks by amount reduction from 50 to 15 g/$m\ell$ kanamycin for in vitro selection to minimize phytotoxic effects and to increase callus growth and regeneration efficiency. Among transgenic plants, 62 out of 106 transgenic poplars (58.5%) showed abnormal phenotypes such as severe serrated leaves and light leaf coloration. Indigo staining with X-gluc proved indirectly the restoration of Gus enzyme function and the presence of Ac in poplar genome by PCR. Southern analysis indicated the transposition and existence of Ac element in poplar genomes. In this research, an Agrobacterium-mediated transformation system in poplar species was developed and identified that Ac derived from maize can be excised and trans posed into other poplar genomes.

• Korean Journal of Environmental Agriculture
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• v.39 no.3
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• pp.273-279
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• 2020

BACKGROUND: After transplanting, the recent abnormal low temperature caused physiological disorders of pepper seedlings. This study was conducted to evaluate the effects of UV-A LED, a physical elicitor, on the chilling tolerance of pepper seedlings. METHODS AND RESULTS: Seedlings were continuously irradiated with 370 and 385 nm UV-A LEDs with 30 W·m^-2 for 6 d. After that, seedlings were exposed to 4℃ for 6 h and then recovered under the normal growing condition for 2 d. There were no significant differences in growth characteristics of UV-A treatments compared to the control. Fv/Fm values of two UV-A treatments were below 0.8. Electrolyte leakage in the control was increased by chilling stress, while 385 nm UV-A had the significantly lowest value. Total phenolic content and antioxidant capacity of two UV-A treatments significantly increased due to UV-A radiation. However, total phenolic content and antioxidant capacity of the control increased due to chilling stress and tended to decrease again during the recovery time. CONCLUSION: We confirmed that UV-A light was effective to induce the chilling tolerance of pepper seedling, and the supplemental radiation of 385 nm UV-A LED before transplanting could be used as a cultivation technique to produce high quality pepper seedlings.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.04a
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• pp.121-121
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• 1995

This study was conducted to examine novel Pt(II) complexes, (KHPC-002: [Pt(trans-1-dach) (DPPE)]. 2N0$_3$, KHPC-005: [Pt(cis-dach) (DPPE)] 2NO$_3$ and KHPC-006: [Pt(cis-dach) (DPPP)]. 2NO$_3$) for their acute toxicities. In male and female mice given a single intraperitoneal administration of KHPC-002, KHPC-005 and KHPC-006, we determined that LD$\_$50/ values of Pt(II) complexes were 295.5mg/kg(M), 350.4mg/kg(F) : KHPC-002, 596.5mg/kg(M), 674,8mg/kg(F): KHPC-005, 158.7mg/kg(M), 157.7mg/kg(F); KHPC-006, respectively. The signs of toxicity in mice observed fellowing the administration of these compounds included the followings: decreased mortor activity: abnormal gait: salviation, trasient decreased body weight. There were no treatment related specific changes in growth examination.

• Molecules and Cells
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• v.26 no.3
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• pp.270-277
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• 2008

This study addresses the physiological functions of the Ran-binding protein homolog NbRanBP1 in Nicotiana benthamiana. Virus-induced gene silencing (VIGS) of NbRanBP1 caused stunted growth, leaf yellowing, and abnormal leaf morphology. The NbRanBP1 gene was constitutively expressed in diverse tissues and an NbRanBP1:GFP fusion protein was primarily localized to the nuclear rim and the cytosol. BiFC analysis revealed in vivo interaction between NbRanBP1 and NbRan1 in the nuclear envelope and the cytosol. Depletion of NbRanBP1 or NbRan1 reduced nuclear accumulation of a NbBTF3:GFP marker protein. In the later stages of development, NbRanBP1 VIGS plants showed stress responses such as reduced mitochondrial membrane potential, excessive production of reactive oxygen species, and induction of defense-related genes. The molecular role of RanBP1 in plants is discussed in comparison with RanBP1 function in yeast and mammals.

• Journal of Microbiology
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• v.45 no.1
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• pp.34-40
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• 2007

In budding yeast, G2/M transition is tightly correlated with bud morphogenesis regulated by Swel and septin that plays as a scaffold to recruits protein components. BNI5 isolated as a suppressor for septin defect is implicated in septin organization and cytokinesis. The mechanism by which Bni5 regulates normal septin function is not completely understood. Here, we show that Bni5 phosphorylation is required for mitotic entry regulated by Swel pathway. Bni5 modification was evident from late mitosis to G1 phase, and CIP treatment in vitro of affinity-purified Bni5 removed the modification, indicative of phosphorylation on Bni5. The phosphorylation-deficient mutant of BNI5 (bni5-4A) was defective in both growth at semi-restrictive temperature and suppression of septin defect. Loss of Bni5 phosphorylation resulted in abnormal bud morphology and cell cycle delay at G2 phase, as evidenced by the formation of elongated cells with multinuclei. However, deletion of Swel completely eliminated the elongated-bud phenotypes of both bni5 deletion and bni5-4A mutants. These results suggest that the bud morphogenesis and mitotic entry are positively regulated by phosphorylation-dependent function of Bni5 which is under the control of Swel morphogenesis pathway.

• Korean Journal of Head & Neck Oncology
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• v.35 no.2
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• pp.61-65
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• 2019

Hamartomas are non-neoplastic malformations or congenital errors of tissue development. Hamartoma is composed by an excessive growth of mature tissue present in wrong proportions and abnormal arrangements. The lesion usually presents as a submucosal mass with ill-defined margins. Hamartoma occurs in all areas of the body, especially in the liver, spleen, kidney and lung. However, hamartoma is very rare in the head and neck. Presenting symptoms of hamartoma are typically vague and nonspecific. Treatment of hamartomas consists of adequate surgical excision. We present a 59 year-old male patient who presented with submental swelling. Malignancy could not be ruled out with preoperative radiologic examination, so surgical excision was planned. The mass was excised with transcervical approach. Histopathologic examination has confirmed the mass as a mucinous gland adenomatoid hamartoma.

• Journal of the Korean Society for Heat Treatment
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• v.21 no.6
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• pp.287-292
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• 2008

Dissolution behavior of complex carbonitrides in a Nb-Ti-V microalloyed steel was quantitatively examined by electrical resistivity, transmission electron microscopy (TEM), and optical microscopy. The electrical resistivity increased with solution treatment temperature up to $1250^{\circ}C$ for a holding time of 15 min. But, an increasing rate of electrical resistivity with temperature was obviously decreased above $1150^{\circ}C$. As the solution treatment temperature increases, irregular shaped Nb-rich carbonitrides disappear and cuboidal Ti-rich carbonitrides are observed. Abnormal grain growth occurs above $1250^{\circ}C$ for a holding time of 15 min. The optimal solution treatment temperature of a Nb-Ti-V microalloyed steel was determined as $1200^{\circ}C$ for a holding time of 15 min.

• Advances in pediatric surgery
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• v.20 no.2
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• pp.65-68
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• 2014

Congenital duodenal obstruction is a one of the emergent surgical conditions in neonates. Almost of them were diagnosed with double-bubble sign in prenatal ultrasonography. However, partial obstruction caused from duodenal web could be overlooked. We reported a duodenal web in early childhood. A three-year-old girl visited at our pediatric clinic for constipation. She had been showed non-bilious vomiting after weaning meal since 6 months old of her age, but her weight was relevant for 50-75 percentile of growth curve. Barium enema was initially checked, but any abnormal finding was not found. We noticed the severely distended stomach and 1st portion of duodenum. Upper gastrointestinal series revealed partial obstruction in 2nd portion of duodenum. After laparotomy, we found the transitional zone of duodenum and identified a duodenal web via duodenotomy. We performed duodeno-duodenostomy without any injury of ampulla of Vater. She was recovered uneventfully. During 6 months after operation, she does well without any gastrointestinal symptoms or signs, such as vomiting or constipation.

• Journal of the Korean Ceramic Society
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• v.46 no.2
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• pp.131-136
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• 2009

Radio frequency (RF) plasma treatment is studied for the size reduction and the spheroidization of coarse particles to change them into nano-sized powders of spherical shape in MLCC fields. The uni-nanoadditives manufactured by RF plasma processing for high dispersion have been investigated for the effect on core-shell structure in dielectrics of MLCC. Microstructures have been characterized using scanning electron microscope (SEM), transmission electron microscope (TEM) and Electron Probe Micro Analyzer (EPMA). We compared the distribution of core-shell grains between specimens manufactured using uni-nanoadditive and using mixed additive. In addition, the uniformity of rare earth elements in the core-shell structured grains was analyzed. It was shown, from TEM observations, that the sintered specimen manufactured using uni-nanoadditives had more dense small grains with well-developed core-shell structure than the specimen using mixed additives, which had a homogeneous microstructure without abnormal grain growth and shows broad temperature coefficient of capacitance (TCC) curves in all temperature ranges because of well dispersed additives.